Light-Dependent Regulation of Cyanophage MaMV-DH01 Infection in Microcystis aeruginosa FACHB-524.

Cyanophages are widely distributed viruses that specifically infect blue-green algae and play a critical role as biological control agents in aquatic ecosystems. Despite their ecological importance, the effects of light on cyanophage-host interactions are not fully understood. This study aimed to investigate the role of host photosynthesis in different stages of MaMV-DH01 infection, a novel muscle-tailed cyanophage isolated from Donghu Lake that targets Microcystis aeruginosa FACHB524.

Lipid Metabolism and Immune Crosstalk in Fish Gut-Liver Axis: Insights from SOCS8 Knockout and Dietary Stress Models.

Metaflammation, a chronic immune response triggered by metabolic dysregulation, poses significant threats to gut-liver homeostasis in aquaculture species. To understand the progression of metaflammation, it is crucial to examine the role of SOCS8 deficiency in socs8 zebrafish, as this species may serve as a disease model for metabolic disorders due to the gradual dysregulation of immunity, metabolism, and the gut microbiota observed in them. This study examines the immune-metabolic crosstalk in grass carp, subjected to soybean meal-induced enteritis, and in socs8 zebrafish under genetic and dietary stress.

Dissolved organic nitrogen and phosphorus derived from different cyanobacteria regulate distinctly different nitrate reduction pathways in sediments.

The differentiation of the nitrate reduction pathway is of great significance in the ecosystem, as it determines the occurrence form of ecosystem N. In order to explore the impact and mechanism of different algal dissolved organic nitrogen (DON) and dissolved organic phosphorus (DOP) on differentiation of nitrate (NO-N) reduction pathway, small-scale enclosure experiment was conducted to analyze the DON and DOP composition, nutrient level, microbial community composition and NO-N reduction pathway in ponds with Microcystis and Dolichospermum blooms. The main DON produced by Microcystis included lipids and proteins as well as carbohydrate which were readily degradable, whereas the DOP produced by Dolichospermum predominantly consists of readily degradable forms such as carbohydrate and protein.

Unraveling the GHG emission patterns of inland waters in China: impact of water body types, aquatic plant life forms, and water temperature.

Inland water ecosystems play key roles in the production, transportation, transformation, storage, and consumption of global greenhouse gases (GHG). Different water body types exhibit spatial and temporal differences after considering factors such as season and aquatic plant life forms. The results revealed that the annual global warming potential (GWP) (Tg CO-eq yr) from swamps, rivers, lakes, and reservoirs in China were 1382.

Filamentous Microalgae Screening for Carotenoid and Lipid Production: A Promising Candidate with High Ammonium Tolerance for Wastewater Treatment.

High-ammonium wastewater can be simultaneously remediated and valorized through phototrophic cultivation of stress-resilient microalgae. This study evaluated the growth performance of 16 microalgae strains (specific growth rate μ = 0.108-0.

Sialic acid serves as a functional receptor for grass carp reovirus.

Grass carp reovirus (GCRV) causes hemorrhagic disease and substantial economic losses in the aquaculture of grass carp (Ctenopharyngodon idella), a commercially important fish species in China. Although viral entry depends on interactions between viral proteins and host receptors, the specific host molecules mediating this process have not been fully elucidated. Here, we identify cell surface sialic acid (SA) as a critical functional receptor for GCRV.

Newly discovered and conserved role of IgM against viral infection in an early vertebrate.

IgM emerged in jawed vertebrates 500 Mya and remains the most evolutionarily conserved antibody class. However, despite extensive studies on IgM as an ancient antiviral weapon in warm-blooded vertebrates, its role and mechanisms in combating viral infections in early vertebrates remain poorly understood. Here, significant virus-specific sIgM titers are generated in the serum and gut mucus of a teleost fish (largemouth bass) that survive infection, and fish lacking sIgM were more susceptible to viral infection.

Starvation Influences the Microbiota in the Stomach of the Corallivorous Crown-of-Thorns Starfish.

The crown-of-thorns starfish (CoTS, spp.), is responsible for a considerable amount of coral loss in the tropical Indo-Pacific region. After decimating coral populations through predation, it is expected that CoTS will face food scarcity before coral recovery.

pVHL regulates protein stability of the TCF/LEF transcription factor family via ubiquitin-independent proteasomal degradation.

The Wnt/β-catenin signaling pathway plays key roles in development and adult tissue homeostasis by controlling cell proliferation and cell fate decisions. TCF/LEF transcription factors play a pivotal role in this pathway, acting as repressors by recruiting co-repressors in the absence of Wnt signals, and as activators via β-catenin binding in the presence of Wnt signaling. While progress has been made in our understanding of Wnt signaling regulation, the underlying mechanism that regulates the protein stability of the TCF/LEF family is far less clear.

Ultrastructure and Transcriptomic Analysis Reveal Alternative Pathways of Zona Radiata Formation in with Different Spawning Habits.

Spawning diversity plays an essential role in fish survival and reproduction, which contributes to the exceptional diversity of teleosts among vertebrates. Different zona radiata structures reflect the adaptability of fish to the environment of spawning and early embryonic development. The morphological and transcriptional characteristics of fish follicle development between different spawning habits, particularly the zona radiata variations, have been poorly documented.

Genetic Adaptation to Brackish Water and Spawning Season in European Cisco.

How species adapt to diverse environmental conditions is essential for understanding evolution and the maintenance of biodiversity. The European cisco (Coregonus albula) is a salmonid that occurs in both fresh and brackish water, and this together with the presence of sympatric spring- and autumn-spawning lacustrine populations provides an opportunity for studying the genetics of adaptation in relation to salinity and timing of reproduction. Here, we present a high-quality reference genome of the European cisco based on PacBio HiFi long read sequencing and HiC-directed scaffolding.

Rapid geomorphic adjustment to anthropogenic vegetation removal and meander cutoffs: Evidence from a lowland meandering river.

Human interventions, such as vegetation removal and engineering structures, can significantly alter river dynamics, often increasing erosion and flood risk. While many studies have examined the role of vegetation, flood regimes, and channel geometry in river morphodynamics, long-term, reach-scale analyses of channel response to abrupt riparian vegetation removal remain scarce. This study examines channel changes in the meandering Orljava River, Croatia, over the past 55 years, focusing on its response to floods before and after anthropogenic removal of riparian vegetation in 2011.

Functional characterization of the plastidial enzyme GPAT in microalgae and its potential role in lipid biosynthesis.

Microalgae are a rich source of high-value natural products. The green microalga Chlamydomonas reinhardtii has long been used as a model organism for studying lipid metabolism in photosynthetic organisms. Here, we comprehensively characterized the enzymatic activity and substrate preferences of the plastidial glycerol-3-phosphate:acyl-CoA acyltransferase (GPAT1) from C.

Double-Edged Sword: Urbanization and Response of Amniote Gut Microbiome in the Anthropocene.

Projections indicate that the global urban population is anticipated to reach 67.2% by 2050, accompanied by a threefold increase in urban built-up areas worldwide. Urbanization has profoundly transformed Earth's natural environment, notably characterized by the drastic reduction and fragmentation of wildlife habitats.

Effects of landscape fragmentation on floodplain fishes as revealed by species-habitat networks.

How species interact with habitat patches is influenced primarily by habitat configuration (e.g., connectivity) and species' functional traits.

Multiple stressors enhance Microcystis dominance and modulate phycospheric antibiotic resistome in aquatic mesocosm.

Assessing the response of phycospheric antibiotic resistance genes (ARGs) to pollutants such as antibiotics and microplastics (MPs) under the background of climate warming is crucial for understanding ARG fate in aquatic ecosystems. In this work, we conducted mesocosm experiments to evaluate how these stressors influence phytoplankton dominance, phycospheric bacteria, and ARG evolution. Results showed that in Microcystis-dominant ecosystem, a single stressor strengthened Microcystis dominance (up to 82.

Bringing the uncultivated microbial majority of freshwater ecosystems into culture.

Axenic cultures are essential for studying microbial ecology, evolution, and genomics. Despite the importance of pure cultures, public culture collections are biased towards fast-growing copiotrophs, while many abundant aquatic prokaryotes remain uncultured due to uncharacterized growth requirements and oligotrophic lifestyles. Here, we applied high-throughput dilution-to-extinction cultivation using defined media that mimic natural conditions to samples from 14 Central European lakes, yielding 627 axenic strains.

Metagenomic analysis reveals how multiple stressors disrupt virus-host interactions in multi-trophic freshwater mesocosms.

Virus-host interactions are vital to microbiome ecology and evolution, yet their responses to environmental stressors under global change remain poorly understood. We perform a 10-month outdoor mesocosm experiment simulating multi-trophic freshwater shallow lake ecosystems. Using a fully factorial design comprising eight treatments with six replicates each, we assess the individual and combined effects of climate warming, nutrient loading, and pesticide loading on DNA viral communities and their interactions with microbial hosts.

Palmelloid-like structure formation mechanism in Chlorella sorokiniana under ethanol stress.

Although the formation of "palmelloid-like" cells as a response to environmental stress has been sporadically reported in Chlorella sp., the association between morphological and molecular indices has been poorly understood. Hence, this study investigated the morphological and molecular effects of ethanol stress on C.

Loss of distinct functionality during fish community disassembly.

Human-induced environmental changes increase species turnover, typically characterized by native species extirpation and non-native species (NNS) invasions, leading to multiple functional consequences owing to varying species roles and whether losses from extirpation are compensated by newly established NNS. We analysed community functional roles among fish species that persisted, were extirpated or were newly established over 75 years (1940-2015) in 15 lakes in southwest China, using functional diversity indices and trait-based network analyses. While species extirpation rate increased strongly through time, earlier extirpations caused the greatest losses to novel functional diversity.

Differential photosynthetic and metabolic susceptibility of Microcystis aeruginosa and Raphidocelis subcapitata to environmentally relevant levels of Diuron.

The sensitivity of phytoplankton growth to chemical stressors influences the composition and function of phytoplankton communities. In this study, we compared the growth, photosynthetic, and metabolic responses of the cyanobacterium Microcystis aeruginosa FACHB-469 and the green alga Raphidocelis subcapitata FACHB-271 to herbicide diuron. The 5-day growth inhibition test showed the M.

Phylogenetic analysis, tissue expression, and response to temperature variation of TRP genes in Monopterus albus.

The transient receptor potential (TRP) channels are essential in vertebrate sensory nervous systems, responding to various stimuli, including changes in environmental temperature. A recent study suggested that Trpv4 may be involved in temperature-induced sex reversal of Monopterus albus, a hermaphroditic and economically farmed fish. In this study, we obtained a total of 36 TRP genes of M.