Prototheca wickerhamii Causing Toe Infection Presenting Initially as Gouty Arthritis: A Rare Case Report.

Prototheca, a genus of opportunistic pathogenic microalgae, can cause protothecosis in humans and animals, manifesting as cutaneous lesions or disseminated/systemic infections. This report describes a rare case of Prototheca wickerhamii toe infection in a 78-year-old Chinese male, presenting initially as gouty arthritis. The patient, who worked in fish farming with frequent water exposure, had a history of herpes zoster and hypertension.

Unraveling the GHG emission patterns of inland waters in China: impact of water body types, aquatic plant life forms, and water temperature.

Inland water ecosystems play key roles in the production, transportation, transformation, storage, and consumption of global greenhouse gases (GHG). Different water body types exhibit spatial and temporal differences after considering factors such as season and aquatic plant life forms. The results revealed that the annual global warming potential (GWP) (Tg CO-eq yr) from swamps, rivers, lakes, and reservoirs in China were 1382.

Brain functional alterations in type 2 diabetes with obstructive sleep apnea patients: A multicenter resting-state fMRI study.

Objective: This multicenter study aimed to investigate resting-state brain functional alterations in patients with type 2 diabetes mellitus (T2DM) comorbid with obstructive sleep apnea (OSA), and to elucidate the underlying neural mechanisms.

Methods: A total of 139 participants were enrolled from two centers, including 48 healthy controls (HCs), 46 T2DM patients, and 45 T2DM with OSA patients. Resting-state functional magnetic resonance imaging (rs-fMRI) was used to assess brain function using degree centrality (DC), amplitude of low-frequency fluctuation (ALFF), and seed-based functional connectivity (FC).

Connectivity-based localization and mapping of central epilepsy semiology.

Objective: Semiology-based preoperative anatomical hypotheses are necessary, yet comprehensive reports on the semiology and its correlation with central subregions in central epilepsy has still lacked. We wished to identify semiologic subgroups and their correlations with central subregions.

Methods: We retrospectively included 21 patients with central epilepsy identified by stereoelectroencephalography (sEEG).

Regional Transcriptomic Architecture of Glioblastoma Reveals NUCB2 as a Key Orchestrator of Tumour Aggression and Immune Dysfunction.

Glioblastoma (GBM) exhibits remarkable intra-tumoral heterogeneity, which contributes to therapeutic resistance and poor clinical outcomes. In this study, we employed integrative single-cell RNA sequencing analysis across two complementary public datasets encompassing diverse cellular populations from GBM centre and periphery regions to elucidate potential spatial molecular programmes driving tumour progression. Our analyses revealed substantial transcriptomic divergence between anatomically distinct tumour regions, with NUCB2 emerging as significantly upregulated in centre-residing neural progenitor cell-like (NPC-like) tumour cells.

Development and validation of a nomogram for predicting 30-day outcomes in patients with unruptured vertebrobasilar dissecting aneurysms undergoing endovascular treatment: a multicenter cohort study.

Background: Endovascular treatment (EVT) of vertebrobasilar dissecting aneurysms (VBDAs) is associated with high morbidity and mortality, significantly influencing patient prognosis. This study aimed to develop and validate a nomogram for predicting 30-day outcomes in patients with unruptured VBDAs undergoing EVT.

Methods: This retrospective study included 606 patients with unruptured VBDAs who underwent EVT at 10 institutions between January 2015 and April 2025, with 491 in the training cohort and 115 in the validation cohort.

Chitin nanocrystal/carboxymethyl cellulose bilayer emulsion with rigid inner layer and flexible outer shell for ultra-stable encapsulation of fish oil.

The polyunsaturated fatty acids in fish oil are prone to oxidation and have poor dispersibility, which limits their use in the food sector. In this work, oil-in-water emulsions stabilized by chitin nanocrystals (ChNC) were prepared via high-speed homogenization. Anionic carboxymethyl cellulose (CMC) was assembled onto cationic ChNC-stabilized emulsion droplet surfaces via layer-by-layer self-assembly technology to construct ChNC/CMC (Ch-C) bilayer emulsions with rigid inner layer and flexible outer shell structures.

Marine organism-inspired tough and adhesive patch based on thermosensitive quaternized chitin for tissue sealing/repair and hemostasis.

Tissue adhesives have emerged as a promising alternative to conventional sutures and staplers in the management of hemostasis, tissue defect sealing, and wound repair. However, the efficacy of current bio-adhesives in clinical practice is compromised by the limitations, including poor wet adhesion, inadequate mechanical strength, vulnerability to gastrointestinal fluids, and insufficient hemostatic performance. Herein, a marine organism-inspired tough and adhesive patch (MOTAP) was developed to address these challenges.

Edge-centric Brain Connectome Representations Reveal Increased Brain Functional Diversity of Reward Circuit in Patients with Major Depressive Disorder.

Background: Major depressive disorder (MDD) has been increasingly understood as a disorder of network-level functional dysconnectivity. However, previous brain connectome studies have primarily relied on node-centric approaches, neglecting critical edge-edge interactions that may capture essential features of network dysfunction.

Methods: This study included resting-state functional MRI data from 838 MDD patients and 881 healthy controls (HC) across 23 sites.

A facile and stable single-system fluorescent sensor array for the quantification and discrimination of nitrofuran antibiotics.

The misuse of nitrofuran antibiotics (NFAs) poses serious risks to food safety and human health. Thus, it is urgent to establish a rapid and sensitive method for detecting NFAs. In this work, a dual-emission fluorescent material (TbUDPA) was developed by embedding Tb in a Zr-MOF for rapid and sensitive detection of four NFAs, and it successfully detected nitrofurazone (NFZ) residues in real food samples with high recovery rates.

Iminoboronate chemistry enables multifunctional and reprocessable polyphenol-derived vitrimers.

Polyphenol-derived vitrimers offer compelling prospects for sustainable materials owing to their intrinsic recyclability, reprocessability and biodegradability. However, practical development remains constrained by structure degradation under harsh reprocessing conditions and the need for sophisticated modifications of the bio-sourced precursors. Herein, we reported a strategy that integrates commercially available polyphenols and low-molecular-weight PDMS through adaptable iminoboronate chemistry, obviating the need for structural modifications.

Unraveling the pathogenicity role of the novel compound heterozygous mutations of MED25 gene in a Chinese patient with BVSYS.

Introduction: Mediator of RNA polymerase II transcription subunit 25 (), a crucial component of the transcriptional coactivator complex, plays a significant role in the transcription of most RNA polymerase II-dependent genes. Mutations in have been linked to various genetic syndromes, including Basel-Vanagaite-Smirin-Yosef Syndrome (BVSYS) and Intellectual Disability (ID). This study elucidated the molecular mechanism through which compound heterozygous mutations in the gene impaired pre-mRNA splicing, ultimately causing BVSYS.

Investigating the impact of type 2 diabetes mellitus on brain function in obstructive sleep apnea patients using regional homogeneity and seed-based functional connectivity methods.

Objective: This study aims to investigate the effects of Type 2 Diabetes Mellitus (T2DM) on brain function in patients with Obstructive Sleep Apnea (OSA) using Regional Homogeneity (ReHo) combined with seed-based Functional Connectivity (FC) methods.

Materials And Methods: 46 OSA patients, 38 OSA with T2DM patients, and 34 healthy controls (HC) were prospectively recruited. Clinical data were collected from all participants, and neuropsychological testing was performed using the Montreal Cognitive Assessment (MoCA), Mini-Mental State Examination (MMSE), and Epworth Sleepiness Scale (ESS).

Predicting 28-day mortality for patients with acute anterior circulation large vessel occlusion stroke following endovascular treatment in neurology intensive care units.

Background: The clinical utility of the National Institutes of Health Stroke Scale, Glasgow Coma Scale, and modified Rankin Scale scores in predicting prognosis is well established. However, whether the Acute Physiology and Chronic Health Evaluation System II (APACHE II) score can predict mortality in patients with large vessel occlusion stroke (LVOS) admitted to the neurology intensive care unit (NICU) following endovascular treatment (EVT) remains unclear. This study aims to evaluate the ability of the APACHE II score to predict mortality in post-EVT LVOS patients admitted to the NICU.

Copper-Catalyzed Denitrogenative Radical Cyclization of 3-Aminoindazoles with -Cyanoarylacrylamides: Synthesis of Cyanoarylated Quinoline-2,4(1,3)-diones.

A copper-catalyzed denitrogenative radical cyclization of 3-aminoindazoles with -cyanoarylacrylamides has been developed, enabling the efficient synthesis of a variety of cyanoarylated quinoline-2,4(1,3)-diones in moderate to good yields at room temperature. Notably, this strategy overcomes the traditional challenge of the low reactivity of nitrile groups toward radical addition by utilizing cyanoaryl radicals generated from 3-aminoindazoles as reactive intermediates. Mechanism studies indicate that 2-cyanophenyl radicals, generated through the cleavage of two C-N bonds of 3-aminoindazoles, served as the key intermediates initiating the cyclization process.

Microorganisms in mosquitoes for controlling mosquito-borne viral diseases: from lab to field.

Mosquito-borne viral diseases pose a growing global health threat, with over half of the world's population living in high-risk areas. In the absence of effective vaccines or antiviral therapies for most mosquito-borne viruses, vector control remains the primary strategy. However, traditional insecticides face rising resistance and ecological concerns.

Intelligent bacterial platform induces programmed death-ligand 1 lysosomal degradation to enhance antitumor immunity.

Engineered bacteria have emerged as a promising therapeutic strategy for tumor therapy by exploiting the unique features of the tumor microenvironment (TME). In this study, we developed a low-endotoxin Escherichia coli expression system to produce a fusion protein comprising the CXCL12 with the nanobody KN035 Hypoxia and immunosuppression drive the selective colonization of engineered bacteria in tumor tissues, minimizing host toxicity. Unlike conventional PD-L1 antibodies that merely block immune checkpoints, our CXCL12-KN035 fusion protein simultaneously targets the CXCR7 receptor and PD-L1 on tumor cells, inducing receptor internalization and subsequent lysosomal degradation of PD-L1.

Reprogramming of microspore fate via BBM-BAR1 for highly efficient in vivo haploid induction.

Haploid induction (HI) through stress-treated microspore culture has gained significant attention for over half a century, yet the molecular mechanism underlying microspore fate transition for androgenesis remains poorly understood. Here, we demonstrate that microspore-specific expression of BABY BOOM (BBM) is sufficient to induce microspore cell fate transition and in vivo androgenesis in both tobacco and rice, effectively bypassing the requirement for stress treatment. We further identify BBM-activated Androgenesis Regulator 1 (BAR1) as a novel downstream effector of BBM that promotes microspore reprogramming.

Characteristics of the 18F-fluorodeoxyglucose (F-FDG) and [F] 9-fluoropropyl-(+)-dihydrotetrabenazine (F-FP-DTBZ) positron emission tomography (PET) in patients with cognitive impairment in Parkinson's disease.

Aim: Mild cognitive impairment (MCI) is common in Parkinson's disease (PD). This study compared cerebral metabolism and dopaminergic function in PD patients with MCI (PD-MCI) versus those with normal cognition (PD-NC) and healthy controls (HC).

Material And Methods: A total of 78 PD patients (40 PD-MCI, 38 PD-NC) and 28 HC underwent 18F-fluorodeoxyglucose positron emission tomography (F-FDG PET) (metabolism) and [F] 9-fluoropropyl-(+)-dihydrotetrabenazine (F-FP-DTBZ) PET (dopaminergic vesicular transporter).

Targeting PANoptosis: a promising therapeutic strategy for ALI/ARDS.

Acute lung injury (ALI) is a complex, high-mortality pulmonary disease triggered by multiple etiological factors, potentially progressing to acute respiratory distress syndrome (ARDS). During the development of ALI/ARDS, a key pathological feature involves the disruption of the intact alveolar-capillary barrier, which is formed by alveolar epithelium, pulmonary interstitium, and microvascular endothelium. Under physiological conditions, cell death removes excess or dysfunctional cells, defends against pathogenic microorganisms, and thus plays a protective role while maintaining homeostasis.

Multiple Lignocellulosic Inhibitor-Tolerant Strains Developed by Evolutionary Engineering and CRISPR/Cas9 Gene Editing Technology.

Through evolutionary engineering strategies, scientists have successfully cultivated multiple strains of with enhanced tolerance, demonstrating significant potential in improving resistance. In this study, was continuously cultured for 80 days in a medium containing lignocellulosic inhibitors (furfural, acetic acid, and vanillin). The evolved strain, , exhibited 12 h reduction in lag phase under multiple stress conditions and 17% increase in the ethanol conversion rate.

Regulating the Donor and Acceptor Interaction Mode in Metal-Organic Frameworks for HO Photosynthesis.

Development of novel photocatalysts for hydrogen peroxide (HO) production from water and oxygen is gathering great attention, but still suffers from inefficient photogenerated charge mobility. Reticular chemistry in metal-organic frameworks (MOFs) provides a promising platform to enhance the charge-transfer kinetics by assembling a donor-acceptor (D-A) system. Here, we designed and synthesized a series of D-A type MOFs (UiO-67-NB, UiO-67-PE/BB, and UiO-67-PE/NB) via regulating the interaction mode of donor and acceptor.

Dietary microbes and functional dyspepsia: modulating the gut microecology for therapeutic benefit.

The pathogenesis of functional dyspepsia (FD) is closely associated with intestinal microecological alterations. Dietary microorganisms, capable of modulating gut microecology and thereby influencing gastrointestinal function, are being explored as a promising therapeutic strategy against FD. However, the precise mechanisms underlying how dietary microbes exert beneficial effects through microecological modulation, along with therapeutic protocols, remain incompletely defined.

[Blood glucose-lowering mechanism of Poria aqueous extract by UPLC-Q-TOF-MS/MS combined with network pharmacology and experimental verification].

Ultra performance liquid chromatography-quadrupole-time-of-flight-mass spectrometry/mass spectrometry(UPLC-Q-TOF-MS/MS), network pharmacology, and animal experiments were integrated o explore the blood glucose-lowering effects and mechanisms of Poria aqueous extract. Firstly, the active components of Poria aqueous extract were identified by UPLC-Q-TOF-MS/MS. Subsequently, network pharmacology was employed to predict the blood glucose-lowering components and mechanisms of Poria aqueous extract.