Predicting 28-day mortality for patients with acute anterior circulation large vessel occlusion stroke following endovascular treatment in neurology intensive care units.

Background: The clinical utility of the National Institutes of Health Stroke Scale, Glasgow Coma Scale, and modified Rankin Scale scores in predicting prognosis is well established. However, whether the Acute Physiology and Chronic Health Evaluation System II (APACHE II) score can predict mortality in patients with large vessel occlusion stroke (LVOS) admitted to the neurology intensive care unit (NICU) following endovascular treatment (EVT) remains unclear. This study aims to evaluate the ability of the APACHE II score to predict mortality in post-EVT LVOS patients admitted to the NICU.

Clinical characteristics and caloric testing in patients with light or heavy cupula of the horizontal semicircular canal.

Objectives: Persistent direction-changing positional nystagmus (DCPN) and null point (NP) are characteristic of cupulopathy of the horizontal semicircular canal (HSC). The cupulopathy can manifest as HSC-light cupula (HSC-Lcu) (geotropic DCPN) and HSC-heavy cupula (HSC-Hcu) (apogeotropic DCPN) in the supine roll test (SRT). Whether the affected side of cupulopathy could be based on the nystagmus intensity in the SRT is controversial.

The Gene Family in Cucumber ( L.): Genome-Wide Identification and Gene Expression Analysis in Phytohormone and Abiotic Stress Response.

The auxin efflux transporter PIN protein plays a crucial role in the asymmetric distribution of auxin on the plasma membrane, influencing the growth and development of plant organs. In this study, we identified nine members of the gene family in the cucumber genome, which could be classified into five phylogenetic groups. These genes have diverse structures but conserved transmembrane domains.

Guillain-Barré Syndrome During the Outbreak of Omicron in Southern China: A Multicenter Case-Control Study.

Purpose: The largest nationwide outbreak of Omicron, a severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variant, occurred between December 2022 and February 2023 in China. This multicenter case-control study investigated the clinical features of GBS during this period.

Methods: The clinical characteristics of patients diagnosed with GBS associated with SARS-CoV-2 were assessed during an Omicron outbreak at 14 referral hospitals in Hubei Province, Southern China.

Transcriptomic and Metabolomic Insights into Plant Hormone Modulation and Secondary Metabolite Accumulation in Basil Under Far-Red and Ultraviolet-A Light.

In this study, red-blue light (7R3B) was used as the control (CK), while far-red (FR) and ultraviolet-A (UVA) light were supplemented to evaluate their effects on basil growth. The results showed that the FR treatment promoted plant height, stem diameter, and biomass, but reduced chlorophyll and carotenoid content, while the UVA treatment increased stem diameter and chlorophyll b content. Meanwhile, transcriptomic and metabolomic analyses were employed to examine changes in gene expression and metabolite accumulation in basil.

Joint effects of physical activity and sleep quality on all-cause and cardiovascular disease mortality in stroke survivors: a population-based cohort study from the UK-Biobank.

Background: Stroke survivors exhibit a higher prevalence of sleep disturbances and physical activity (PA) deficiencies. The joint effects of the two behaviors on mortality risk among stroke survivors remains unclear. This study aimed to explore the joint association of PA and sleep quality with the all-cause and cardiovascular disease ( CVD ) mortality risk in stroke survivors.

Comprehensive characterization and expression profiling of sucrose phosphate synthase (SPS) and sucrose synthase (SUS) family in Cucumis melo under the application of nitrogen and potassium.

Background: Sugars are not only important biomacromolecules that play vital roles in plant growth, development and environmental stress tolerance, but they also provide carbon skeletons for the synthesis of other macromolecules, such as proteins and nucleic acids. Sugar-related proteins play key roles in the movement of sugars from source tissues (such as leaves) to sink tissues (such as fruits), ultimately influencing fruit development. However, the evolutionary dynamics of this important sugar-related gene family in the Cucumis melo (C.

Vestibular and Ocular Motor Properties in Cerebellar Infarction.

Background: The study aimed to determine the patterns of the vestibular and ocular motor findings in cerebellar infarction (CI).

Methods: We retrospectively analyzed vestibular and ocular motor test results in 23 CI patients and 32 acute unilateral vestibulopathy (AUVP) patients.

Results: Among CI cases, the posterior inferior cerebellar artery (PICA) was the most commonly affected territory.

Small Molecule Drugs Triggered the Activation of Macrocycle Masked Proteins.

On-demand activation of prodrugs represents an emerging and fast developing strategy to improve the therapeutic index of certain drugs. However, strategies to generate protein-based prodrugs with controllable activation are still limited. Here, we present a supramolecular masking strategy that enables on-demand activation of macrocycle-masked proteins with Food and Drug Administration (FDA)-approved oral drugs.

Effects of Far-Red Light and Ultraviolet Light-A on Growth, Photosynthesis, Transcriptome, and Metabolome of Mint ( Briq.).

To investigate the effects of different light qualities on the growth, photosynthesis, transcriptome, and metabolome of mint, three treatments were designed: (1) 7R3B (70% red light and 30% blue light, CK); (2) 7R3B+ far-red light (FR); (3) 7R3B+ ultraviolet light A (UVA). The results showed that supplemental FR significantly promoted the growth and photosynthesis of mint, as evidenced by the increase in plant height, plant width, biomass, effective quantum yield of PSII photochemistry (F'/F'), maximal quantum yield of PSII (F/F), and performance index (PI). UVA and CK exhibited minimal differences.

Computation-Guided Discovery of Diazole Monosubstituted Tetrazines as Optimal Bioorthogonal Tools.

Monosubstituted tetrazines are important bioorthogonal reactive tools due to their rapid ligation with -cyclooctene. However, their application is limited by the reactivity-stability paradox in biological environments. In this study, we demonstrated that steric effects are crucial in resolving this paradox through theoretical methods and developed a simple synthetic route to validate our computational findings, leading to the discovery of 1,3-azole-4-yl and 1,2-azole-3-yl monosubstituted tetrazines as superior bioorthogonal tools.

Clinical characteristics and lateralization of the horizontal semicircular canal light cupula.

Introduction: Positional vertigo and nystagmus are the main symptoms and signs of dizziness, respectively. Despite the clinical utility of the supine roll test (SRT) and null point (NP) in diagnosing light cupula, a type of positional vertigo, there exists a notable gap in the literature concerning the comprehensive evaluation of lateralization values based on various nystagmus characteristics and the intensity of direction-changing positional nystagmus (DCPN) in the SRT, particularly in comparison to the NP. Additionally, limited data on abnormal canal paresis (CP) in light cupula patients underscores the need for further research with a larger patient population to elucidate this mechanism.

Tracking endogenous proteins based on RNA editing-mediated genetic code expansion.

Protein labeling approaches are important to study proteins in living cells, and genome editing tools make it possible to tag endogenous proteins to address the concerns associated with overexpression. Here we established RNA editing-mediated noncanonical amino acids (ncAAs) protein tagging (RENAPT) to site-specifically label endogenous proteins with ncAAs in living cells. RENAPT labels protein in a temporary and nonheritable manner and is not restricted by protospacer adjacent motif sequence.

Comprehensive review: Effects of climate change and greenhouse gases emission relevance to environmental stress on horticultural crops and management.

Global climate change exerts a significant impact on sustainable horticultural crop production and quality. Rising Global temperatures have compelled the agricultural community to adjust planting and harvesting schedules, often necessitating earlier crop cultivation. Notably, climate change introduces a suite of ominous factors, such as greenhouse gas emissions (CGHs), including elevated temperature, increased carbon dioxide (CO) concentrations, nitrous oxide (NO) and methane (CH) ozone depletion (O), and deforestation, all of which intensify environmental stresses on crops.

Integrated Analysis of the Transcriptome and Metabolome of Revealed Regulatory Mechanism under Heat Stress.

Affected by global warming; heat stress is the main limiting factor for crop growth and development. prefers cool weather, and heat stress has a significant negative impact on its growth, development, and metabolism. Understanding the regulatory patterns of heat-resistant and heat-sensitive varieties under heat stress can help deepen understanding of plant heat tolerance mechanisms.

The psychological stress response of COVID-19 to medical staff and prevention: A large sample study from China.

Objectives: In the face of the COVID-19 pandemic, medical staff in China were more likely to suffer from psychological problems. By investigating the actual state of psychological stress response of medical staff during the COVID-19 outbreak, the study discussed and analyzed the influencing factors of different psychological states in order to prevent the occurrence of serious adverse emotional events in medical staff.

Methods: In the Xiangyang Central Hospital, 1,466 medical staff members have adopted the Psychological Questionnaire for Emergencies Events of Public Health (PQEEPH), which includes questions about depression, neurasthenia, fear, obsessive anxiety, and hypochondriac disorders.

Study on nitrogen demand model in pakchoi ( ssp. L.) based on nitrogen contents and phenotypic characteristics.

Introduction: In precision agriculture, the diagnosis of the nitrogen (N) nutrition status based on the plant phenotype, combined effects of soil types, various agricultural practices, and environmental factors which are essential for plant N accumulation. It helps to assess the N supply for plants at the right time and optimal amount to ensure high N use efficiency thereby reducing the N fertilizer applications to minimize environmental pollution. For this purpose, three different experiments were performed.

Phytotoxic effects of chemically synthesized copper oxide nanoparticles induce physiological, biochemical, and ultrastructural changes in Cucumis melo.

Nanotechnology has achieved great attention due to its impressive performance especially engineered nanoparticles (ENPs). Copper-based nanoparticles offer favorable development in the fabrication of agrochemicals including fertilizers and pesticides in the field of agriculture. However, their toxic impact on melon plants (Cucumis melo) still needs to be investigated.

A green and environmental sustainable approach to synthesis the Mn oxide nanomaterial from Punica granatum leaf extracts and its in vitro biological applications.

Pathogenic fungal infections in fruit cause economic losses and have deleterious effects on human health globally. Despite the low pH and high water contents of vegetables and fresh, ripened fruits, they are prone to fungal and bacterial diseases. The ever-increasing resistance of phytopathogens toward pesticides, fungicides and bactericides has resulted in substantial threats to plant growth and production in recent years.

A model selection criterion for clustered survival analysis with informative cluster size.

We propose a model selection criterion for correlated survival data when the cluster size is informative to the outcome. This approach, called Resampling Cluster Survival Information Criterion (RCSIC), uses the Cox proportional hazards model that is weighted with the inverse of the cluster size. The RCSIC based on the within-cluster resampling idea takes into account the possible variability of the within-cluster subsampling and the possible informativeness of cluster sizes.

Differential Metabolic Responses of Lettuce Grown in Soil, Substrate and Hydroponic Cultivation Systems under NH/NO Application.

Nitrogen (N) is an essential element for plant growth and development. The application of a balanced and optimal amount of N is required for sustainable plant yield. For this, different N sources and forms are used, that including ammonium (NH) and nitrate (NO).

Efficient generation of locus-specific human CAR-T cells with CRISPR/cCas12a.

We recently developed a system to create human chimeric antigen receptor (CAR)-T cells using conjugated Cas12a (cCas12a) in which Cas12a is covalently linked to its CRISPR RNA (crRNA). This protocol describes site-specific modification of Cas12a and the preparation of Cas12a-crRNA complex using bio-orthogonal chemistry, followed by CAR-T cell generation through electroporation and AAV infection. This system shows robust editing efficiency in human cells and can be used for precisely targeted, highly efficient integration of CAR genes into T cell genome.

Physiological and Transcriptomic Analysis Reveals the Responses and Difference to High Temperature and Humidity Stress in Two Melon Genotypes.

Due to the frequent occurrence of continuous high temperatures and heavy rain in summer, extremely high-temperature and high-humidity environments occur, which seriously harms crop growth. High temperature and humidity (HTH) stress have become the main environmental factors of combined stress in summer. The responses of morphological indexes, physiological and biochemical indexes, gas exchange parameters, and chlorophyll fluorescence parameters were measured and combined with chloroplast ultrastructure and transcriptome sequencing to analyze the reasons for the difference in tolerance to HTH stress in HTH-sensitive 'JIN TAI LANG' and HTH-tolerant 'JIN DI' varieties.

Objective findings in patients with multi-canal benign paroxysmal positional vertigo.

Objective: The study aimed to investigate the clinical characteristics of patients with multi-canal benign paroxysmal positional vertigo (MC-BPPV).

Methods: We performed a retrospective analysis of 927 patients with BPPV who were admitted to our hospital between January 1, 2016 and December 31, 2019. The clinical data of all patients were collected.