Fungal symbiont Mycena complements impaired nitrogen utilization in Gastrodia elata and supplies indole-3-acetic acid to facilitate its seed germination.

Nitrogen and auxin uptake plays pivotal roles in seed germination and development. Gastrodia elata, a fully mycoheterotrophic plant, depends entirely on its symbiotic association with Mycena for early growth and seed germination. The process by which Mycena enables the supply of nitrogen nutrients and auxin, which are deficient in G.

Glehnia littoralis polysaccharides alleviate lung and intestinal mucosal barrier damage in rats with ulcerative colitis via aryl hydrocarbon receptor activation.

This study aimed to investigate the regulatory effects of Glehnia littoralis polysaccharides (GLP) on lung and intestinal mucosal barriers in rats with ulcerative colitis (UC). The results demonstrated that GLP inhibited the levels of TNF-α, IL-6, and MPO in colon tissues, alleviated colonic pathological damage, and reduced the expression of TNF-α, IL-6, IL-1β, and MPO in lung tissues, thereby ameliorating lung injury secondary to intestinal inflammation. Further analysis revealed that GLP restored the integrity of lung and intestinal mucosal barriers by upregulating the expression of mucin, ZO-1, and Occludin.

Population pharmacokinetics and pharmacodynamics of meropenem in critically ill patients with renal impairment or on continuous renal replacement therapy.

Purpose: This study aimed to characterize the pharmacokinetics (PK) of meropenem in patients with renal impairment or on continuous renal replacement therapy (CRRT), investigate the factors contributing to PK variability, and evaluate the performance of different dosing regimens to optimize the pharmacokinetics/pharmacodynamics PK/PD) targets.

Methods: This study enrolled patients with severe infections and renal impairment who received meropenem treatment at the Second Affiliated Hospital of Soochow University. Participants were grouped according to whether CRRT was used.

Comparison of Combined Letrozole and Clomiphene Citrate With Gonadotropin Microstimulation Protocols on Fertility Outcomes.

Purpose: Large-scale data comparing the effects of combined letrozole (LE) and clomiphene citrate (CC) with gonadotropin (Gn) microstimulation protocols on pregnancy outcomes are lacking. This study aimed to compare the effects of CC + Gn and LE + Gn microstimulation protocols on fertility outcomes.

Methods: A retrospective cohort study was conducted to include infertile patients between January 1, 2018, and December 31, 2022.

Application of Modified Early Warning Score System (MEWS) in the Assessment of Disease Condition and Prognosis of Patients After Neurosurgical Procedure or Intervention.

Objective: To evaluate the safety and clinical utility of the corrected Modified Early Warning Score (MEWS) system in predicting postoperative clinical deterioration and long-term prognosis in neurosurgical patients.

Methods: A prospective cohort study was conducted on 344 neurosurgical patients admitted between December 2021 and April 2022. Physiological parameters including axillary temperature, respiratory rate, heart rate, systolic blood pressure, arterial oxygen saturation, and consciousness level were systematically recorded.

Prenatal linuron exposure disrupts adrenal steroidogenesis and induces adrenal insufficiency in male rat offspring.

Linuron, a commonly used agricultural herbicide in certain regions, has raised concerns due to its endocrine toxicity in humans and wildlife. While its gonadal toxicity is established, its direct impact on prenatal adrenal development remains unexplored. Pregnant Sprague-Dawley rats received linuron doses (0, 25, 50, 100 mg/kg/day) by oral gavage from gestational days 12-21.

Identification and functional analysis of novel precursor genes in cyclic peptide biosynthesis in Pseudostellaria heterophylla.

Background: , a member of the Caryophyllaceae family, is widely used in traditional Chinese medicine due to its bioactive cyclic peptides (CPs) with immunomodulatory functions. Caryophyllaceae- like CPs, one of the largest types plant-derived CPs, typically consist of 5–12 amino acids and are derived from ribosomally synthesized peptide precursors. The diversity of CPs arises from variations in their core peptide sequences.

Reduced serum iron levels predict poor prognosis in pulmonary mucormycosis patients: a prospective, case‒control study.

Pulmonary mucormycosis (PM) and invasive pulmonary aspergillosis (IPA) are both invasive pulmonary fungal infections with similar clinical manifestations. Few studies have focused on the expression of serum iron in patients with PM and IPA. A prospective, case‒control study was conducted between January 2023 and December 2024.

Clinical characteristics and caloric testing in patients with light or heavy cupula of the horizontal semicircular canal.

Objectives: Persistent direction-changing positional nystagmus (DCPN) and null point (NP) are characteristic of cupulopathy of the horizontal semicircular canal (HSC). The cupulopathy can manifest as HSC-light cupula (HSC-Lcu) (geotropic DCPN) and HSC-heavy cupula (HSC-Hcu) (apogeotropic DCPN) in the supine roll test (SRT). Whether the affected side of cupulopathy could be based on the nystagmus intensity in the SRT is controversial.

Triple-Enhanced Absorption Anisotropy in Arrayed Core-Shell Nanowire Design.

One-dimensional semiconductor nanowires (NWs) are natural polarization photodetectors, thanks to their optical absorption anisotropy and efficient electrical transport channels. However, ultrathin NWs beyond practical fabrication capabilities are often required to achieve the desired polarization sensitivity. Here, we demonstrate a configuration of planar arrayed core-shell NW architectures with substantially enhanced absorption dichroism for linearly polarized light.

Reversible modulation of reconfigurable topological optical bistability with dual-band in mid-infrared.

We present a flexible scheme enabling reversible switching of topological states and dynamic modulation of optical bistability in a fixed micro-nano nonlinear optical system. The system realizes the optical modulation of reconfigurable topological edge states through phase transition-induced optical property changes in GeSbTe. The third-order nonlinear optical effects of the system are effectively enhanced by local field enhancement in the dual-band via reconfigurable topological edge states, which have a positive impact on the realization of dual-channel modulated optical bistability.

Herbal Research, Phytochemistry, Pharmacology, Comprehensive Utilization, and Quality Control of Hemp Seed: A Comprehensive Review.

Background: Cannabis sativa L. is an annual herbaceous plant in the genus Cannabis of the family Cannabaceae. Hempseed, a traditional Chinese medicine, is the dried and ripe decorticated fruit of C.

A phosphoprotein gene RT-qPCR assay for detecting all lineages of peste des petits ruminants virus.

Peste des petits ruminants (PPR) is a highly contagious disease caused by peste des petits ruminants virus (PPRV). PPRV is classified into four lineages based on the nucleocapsid (N) or fusion (F) genes. We established a TaqMan quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) assay using a pair of primers and a probe based on the phosphoprotein (P) gene.

Fabrication of Robust Superhydrophobic Surfaces on 3D-Printed Polymer Scaffolds via Confined Phase Separation.

3D printing enables the fabrication of complex, customizable biomedical polymer structures. The integration of superhydrophobicity─known for its unique self-cleaning, antibacterial, and anticoagulant properties─can significantly enhance the functionality of 3D-printed polymer in biomedical applications. However, conventional coating-based approaches for imparting superhydrophobicity suffer from poor mechanical stability due to weak adhesion between the coating layer and the substrate.

Detection of Lineage IV Peste Des Petits Ruminants Virus by RT-qPCR Assay via Targeting the Hemagglutinin Gene.

Peste des petits ruminants virus (PPRV) has been classified into four lineages based on the nucleocapsid and fusion genes, with lineage IV strains being the most widely distributed. In Africa, recent epidemiological data revealed that PPRV lineage IV is increasingly displacing other lineages in prevalence, suggesting a competitive advantage in viral transmission and adaptability. Moreover, a lineage IV strain was the only confirmed strain in Europe and Asia.

Design of Mn(1,4-DO2A) derivatives as stable and inert contrast agents for magnetic resonance imaging.

Manganese(II) is considered a valuable alternative to gadolinium(III) in developing contrast agents (CAs) for magnetic resonance imaging (MRI). However, due to the labile nature of common Mn(II) complexes, designing ligands that enable stable and inert complexation is essential before clinical application. Mn(1,4-Et4DO2A) bearing four ethyl groups, is found to have a log K as high as 17.

Hsp60 lactylation promotes mitochondrial dysfunction and trophoblast apoptosis in preeclampsia.

Preeclampsia is characterized by placental hypoxia and metabolic reprogramming toward glycolysis, leading to increased lactate production and protein lactylation. This study investigated the role of heat shock protein 60 (Hsp60) lactylation in preeclampsia pathophysiology. Using placental tissue microarrays and HTR-8/SVneo trophoblast cells, we found that Hsp60 undergoes aberrant lactylation in preeclamptic placentas, particularly under hypoxic conditions.

Optimal intrapleural therapy with alpha-hemolytic streptococci bio-product (Mannatide) in controlling malignant pleural effusion: a clustered systematic review and meta-analysis.

Background: Mannatide is an alpha-hemolytic streptococci bio-product. We conducted a comprehensive clustered systematic review and meta-analysis to reveal the clinical values of Mannatide for controlling malignant pleural effusion (MPE), identify the optimal therapeutic regimens, indications and usage, and assess their clinical effectiveness and safety.

Methods: We collected all eligible studies from both Chinese and English databases and clustered them into homogeneous comparisons or therapeutic regimens.

Association between serum direct bilirubin and 90-day mortality in patients with ARDS: A retrospective analysis.

The liver plays a key role in the pathogenesis and resolution of acute respiratory distress syndrome (ARDS). Clinically, elevated serum bilirubin - especially direct bilirubin (DBIL) - is frequently observed in ARDS. This study aimed to evaluate the association between DBIL levels and 90-day mortality in ARDS patients.

Modular Design of a Copper Ion Biosensor Based on the MAPK Signaling Pathway in .

Copper ion poses serious threats to both the environment and human health. To develop a yeast biosensor with reduced background noise and enhanced detection sensitivity, we constructed a quorum-sensing module with amplified positive feedback. This biosensor employs a copper ion-pheromone communication system, which allows haploid -type yeast () to express the α-pheromone gene () under the control of the copper ion-inducible promoter .

Mapping patient safety competency in undergraduate nursing interns: Insights from a latent profile analysis.

Background: High patient safety competency promotes safe clinical practice during undergraduate nursing interns' transition to professional roles and lays a strong foundation for their future careers. Identifying potential subgroups of undergraduate nursing interns based on their patient safety competency, along with the associated factors, may offer targeted insights for developing effective educational interventions.

Aim: To investigate the level of patient safety competency among undergraduate nursing interns using latent profile analysis, identify potential subgroups and their key characteristics, and explore the factors influencing subgroup membership.

PI3K-dependent GAB1/Erk phosphorylation renders head and neck squamous cell carcinoma sensitive to PI3Kα inhibitors.

The hyperactivation of the PI3K pathway in head and neck squamous cell carcinoma (HNSCC) suggests that targeting PI3K is a potential therapeutic strategy. CYH33 is a novel PI3Kα-selective inhibitor discovered by our group, which is currently undergoing a phase I clinical trial (NCT03544905) for the treatment of advanced solid tumors including HNSCC. However, there is an urgent need to elucidate its mechanism of action and improve its efficacy against HNSCC.

Design, Synthesis, and Anticancer Evaluation of Novel 6-Alkynylated Harmine Derivatives.

In order to explore the effect of alkynyl introduction at the 6-position of harmine on the antitumor activity, 23 novel 6-alkynylated harmine derivatives (3a-3w) were synthesized and investigated for their anti-proliferative activity on a panel of cancer cell lines. Compound 3u demonstrated the most potent anti-proliferative activity, with IC = 0.77 µM against MDA-MB-231 cell lines.

Complete chloroplast genome sequence data of Cannabis sativa.

Cannabis sativa, which is an annual erect herb belonging to the Cannabis family, possesses significant medicinal value. The entire genome of the cannabis chloroplast was sequenced using the Illumina NovaSeq 6000 on the Illumina high-throughput sequencing platform. The circular genome has a size of 153,871 base pairs (bp), consisting of two inverted repeats (IRs) that are 26,010 bp each, a large single copy region (LSC) measuring 84,038 bp, and a small single copy region (SSC) of 17,813 bp.