Integrative blood transcriptomic and metabolomic profiling reveals biomarkers of natural heat tolerance in Holstein cows.

Heat stress poses a major threat to dairy cattle productivity, particularly in high-producing Holstein cows. To identify robust biomarkers of thermotolerance, we employed an integrative strategy combining physiological phenotyping, blood metabolite profiling, and transcriptomic analysis. A total of 120 lactating Holstein cows were evaluated under natural summer heat conditions using rectal temperature, respiratory rate, salivation index, serum HSP70, cortisol, potassium levels, and milk production.

A meta analysis of the acupoint catgut embedding in the treatment of functional constipation.

Objective: To evaluate the efficacy and safety of acupoint embedding for FC by meta-analysis, in order to provide evidence-based medical evidence for clinical practice.

Methods: A comprehensive literature search was conducted in China National Knowledge Infrastructure, WanFang, VIP, PubMed, Web of Science and The Cochrane Library for randomised controlled trials (RCTs) on ACE for FC published from inception to November 2024. The included studies were assessed for quality using the modified Jadad scale, and statistical analysis was performed using RevMan 5.

Asymmetric active-site on heterogeneous single-atom alloy metallene boost fenton-like reaction for sustainable water purification.

A precise modulation of heterogeneous catalysts in structural and surface properties promises the development of more sustainable advanced oxidation water purification technologies. However, the poor catalyst stability due to covering of surface-active sites by oxidation intermediates remains a key bottleneck to their practical applications. Herein we propose a simple defect-induced in-situ single-atom anchoring strategy to overcome this challenge by creating unique asymmetric active-sites on the catalyst surface.

Optimizing invasive strategies for necrotizing pancreatitis: A Bayesian network analysis of randomized controlled trials.

Background: necrotizing pancreatitis is a severe complication of acute pancreatitis, often requiring invasive interventions to manage its high mortality and morbidity. The optimal timing and type of invasive procedures remain uncertain, necessitating a systematic evaluation to guide clinical decision-making.

Methods: A systematic review and Bayesian network meta-analysis were conducted following the PRISMA guidelines.

Cost and cost-effectiveness analysis of point-of-care ultrasound (POCUS) in obstetrics: a systematic review protocol.

Introduction: Facing the profound health impacts and economic challenges posed by the high maternal mortality ratio worldwide, point-of-care ultrasound (POCUS) has shown its potential to enhance diagnostic efficiency, reduce healthcare costs and improve health outcomes. However, concerns regarding the explicit and implicit costs as well as actual clinical outcomes of POCUS also exist. This systematic review protocol aims to synthesise existing evidence regarding the costs and cost-effectiveness of POCUS in obstetric care to provide a clearer understanding of its economic and clinical impact, ultimately guaranteeing global maternal and neonatal health and supporting the vision of the 2030 sustainable development goals.

Stress-strain and fracture acoustic emission dataset of high-strength concrete for metro tunnel lining under different stress states.

The maintenance of metro tunnel support structures is crucial for ensuring the safe and efficient operation of urban rail transit. Under complex stress conditions (including tension, compression, shear, torsion), metro tunnel linings are susceptible to various forms of damage, such as cracking, spalling, segment misalignment, and water leakage. These issues pose substantial challenges to tunnel safety and service life.

Electrochemical Sensors Based on Dirac Semimental NiTe in the Detection of SARS-CoV-2.

In the present study, a topological semimetal NiTe-based electrochemical biosensor was designed and fabricated, leveraging the material's inherent topological surface state and conductive bulk properties. The NiTe electrode was fabricated via mechanical exfoliation from a high-quality NiTe single crystal. Owing to its robust layered structure and unique Dirac surface states, the topological semimetal NiTe facilitates rapid electron transfer at the electrode surface, thereby enhancing the sensor's performance.

Profibrotic impact of PAD4-driven macrophage extracellular traps in ulcerative colitis.

The inflammatory polarization of macrophages has been implicated in the pathogenesis of fibrosis in ulcerative colitis (UC). Our previous study found that macrophages in an inflammatory polarization state highly express peptidyl arginine deiminase 4 (PAD4) and are more likely to form macrophage extracellular traps (METs). In this study, we aimed to investigate the role of PAD4-driven METs in promoting intestinal fibrosis in UC.

Preparation and safety assessment of BADGE- histidine adduct from epoxy-coated cans.

Bisphenol A diglycidyl ether (BADGE) from the epoxy-coated canned food can react with proteins/amino acids during high temperature sterilization process. The product formed with histidine (His), BADGE·HO·His, was considered to have potential toxicity. In this study, we successfully prepared and identified this adduct.

Dual-Emission Au-Ag Nanoclusters with Enhanced Photoluminescence and Thermal Sensitivity for Intracellular Ratiometric Nanothermometry.

We report the development of highly luminescent, bovine serum albumin (BSA)-stabilized gold-silver bimetallic nanoclusters (Au-AgNCs@BSA) as a novel platform for high-sensitivity, ratiometric intracellular temperature sensing. Precise and non-invasive temperature sensing at the nanoscale is crucial for applications ranging from intracellular thermogenesis monitoring to localized hyperthermia therapies. Traditional luminescent thermometric platforms often suffer from limitations such as high cytotoxicity and low photostability.

Comparative analysis of key flavonoid synthesis genes in the transcriptome of Cayratia Japonica and therapeutic target prediction for ulcerative colitis : Functional & integrative genomics.

Cayratia japonica is a traditional Chinese herbal plant rich in flavonoid compounds, with anti-inflammatory and anti-cancer properties. Although Cayratia japonica has been used for medicinal purposes for centuries, the mechanisms underlying flavonoid biosynthesis in this plant remain largely unexplored. In this study, combining transcriptomics and bioinformatics, we constructed a transcriptome database for three tissues of Cayratia japonica.

Selective Transport Energy Barriers in Chitin-Assisted Polyamide Membranes for Efficient Cl/SO Sieving.

Nanofiltration (NF) technology is crucial in alleviating global water scarcity through desalination. Overcoming the permselectivity trade-off to improve both the permeance and rejection of polyamide (PA) nanofiltration (NF) membranes beyond the log-log upper bound remains a significant scientific challenge. Herein, we explored advanced ion-transport barriers by constructing a chitin-assisted PA NF membrane, incorporating a biomass-derived carboxylated chitin nanofiber (CO-ChNF) interlayer that simultaneously modulates the pore architecture and surface charge of the PA layer.

Network pharmacology and experimental validation reveal that betulin alleviates 5-fluorouracil-induced intestinal injury by inhibiting intestinal senescence and enhances antitumor efficacy.

5-Fluorouracil (5-FU) remains the first-line chemotherapeutic agent for colorectal cancer. Although 5-FU significantly improves patient survival, its severe gastrointestinal toxicity-particularly intestinal injury and diarrhea-impairs treatment adherence and patient quality of life, often leading to therapeutic failure. Thus, effective interventions to prevent or mitigate these adverse effects are urgently needed.

Network pharmacology and experimental validation reveal clotrimazole alleviates dextran sulfate sodium-induced ulcerative colitis by inhibiting intestinal senescence.

Ulcerative colitis (UC) is one of the spontaneous inflammatory bowel disease, which is characterized by chronic and relapsing intestinal mucosal inflammation that principally affects the colon. Chronic inflammation is a hallmark of senescent tissues, and intestinal tissue gradually appears injured with the occurrence of senescence. Therefore, senescence may aggravate the severity of UC.

Superstretchable and Tough Physical Cross-Linking Hydrogels with Dual pH and Temperature Responsiveness for In Vivo Treatment of -Infected Wounds.

Developing a single hydrogel with comprehensive wound healing functions is challenging. We developed a composite hydrogel wound dressing with thermal and pH responsiveness, demonstrating antibacterial and biocompatible properties in rats with -infected wounds. Sodium alginate cross-linked by Ca served as the continuous phase, while thermosensitive poly(isopropylacrylamide), laponite, lecithin, gelatin, and antibacterial ZIF-8 supported by tannic acid-induced Ag nanoparticles formed the dispersed phase.

Alkaline stress suppresses soybean waterlogging tolerance by exacerbating energy expenditure and ROS accumulation.

To clarify the synergistic inhibition mechanisms of saline-alkali and waterlogging combined stress on soybean physiology, this study systematically analyzed phenotypic traits, photosynthetic characteristics, reactive oxygen species (ROS) metabolism, and energy metabolism under saline-alkali (A + NW), waterlogging (NA + W), and combined stress (A + W). Results demonstrated that saline-alkali stress significantly impaired waterlogging-induced morphological adaptations, with A + W reducing dry weight and adventitious root number compared to W alone. Synergistic photosynthetic damage was observed: net photosynthetic rate (Pn) under A + W decreased markedly versus controls, while chlorophyll b content increased, suggesting PSII light-harvesting complex reorganization to mitigate photoinhibition.

Single-atom catalysts toward electrocatalytic urea synthesis C-N coupling reactions.

Urea, with a global annual production that exceeds 200 million tons, occupies an irreplaceable position in agriculture, pharmaceuticals, and materials science. The conventional Haber-Bosch process and its derivatives are constrained by high energy consumption and considerable carbon emissions. Global urea production, for instance, utilizes approximately 1.

Decoding Antenna Behavior in Metal-Organic Frameworks.

Metal-organic frameworks (MOFs) define a solid-state platform for developing artificial photosystems. Efficient anisotropic exciton migrations in these frameworks entail "antenna behavior" that can power up the distal interior reaction centers (RC), driving charge separation between donor-acceptor pairs. Reminiscent of the natural light-harvesting complex, such processes can achieve high quantum yield by exploiting the vast interior surface of the porous crystallites.

Migration Safety of Perfluoroalkyl Substances from Sugarcane Pulp Tableware: Residue Analysis and Takeout Simulation Study.

The rapid growth of plant-based biodegradable tableware, driven by plastic restrictions, necessitates rigorous safety assessments of potential chemical contaminants like per- and polyfluoroalkyl substances (PFASs). This study comprehensively evaluated PFAS contamination risks in commercial sugarcane pulp tableware, focusing on the residues of five target PFASs (PFOA, PFOS, PFNA, PFHxA, PFPeA) and their migration behavior under simulated use and takeout conditions. An analysis of 22 samples revealed elevated levels of total fluorine (TF: 33.

Survival outcomes and risk factors after lung transplantation in patients with pre-transplant malignancy: a national cohort study.

Background: Pre-transplant malignancy (PTM) is a relative contraindication for lung transplantation (LTx). The proportion of patients with PTM in LTx is increasing annually. We sought to identify modifiable risk factors affecting patient survival and evaluate the prognosis of different types of PTM.

Laparoscopic versus open repeat liver resection for recurrent liver cancer: an updated systematic review and meta-analysis.

Background: Liver resection is still the most effective and curative treatment for recurrent liver cancer, laparoscopic repeat liver resection (LRLR) offers an option for recurrent liver cancer due to invasive advantages. However, multicenter, large-sample population-based LRLR has rarely been reported. We aimed to assess the advantages and drawbacks of LRLR compared with laparoscopic and open surgery for recurrent liver cancer by meta-analysis.

Association of dietary creatine intake from meat protein sources with different types of intestinal problems: insights from NHANES 2005-2010.

Background: Given the growing interest in gut health, particularly in the context of irritable bowel syndrome (IBS), this study investigates the potential effects of dietary creatine intake on measures of gut health. Additionally, in response to anecdotal reports on the internet that have not been corroborated by clinical trials, this research examines the relationship between creatine consumption and gastrointestinal outcomes, aiming to address the existing knowledge gap in this area.

Methods: This study utilized data from the 2005-2010 National Health and Nutrition Examination Survey (NHANES).

Unveiling the Mutual Promotion Mechanism of Adjacent Vacancy Defects Enables High-Performance Perovskite Solar Cells.

The perovskite defect evolution directly impacts the efficiency and stability of perovskite solar cells (PSCs). In this work, the mutual promotion mechanism of adjacent cation and anion vacancies in perovskite is unveiled, which means the cation/anion vacancy induces the adjacent anion/cation vacancy through decreasing the formation energy. This mutual promotion mechanism provides an explanation for the dynamic evolution of defects, and emphasizes the necessity of simultaneously passivating of adjacent defects.

Improving 3D Thin Vessel Segmentation in Brain TOF-MRA via a Dual-space Context-Aware Network.

3D cerebrovascular segmentation poses a significant challenge, akin to locating a line within a vast 3D environment. This complexity can be substantially reduced by projecting the vessels onto a 2D plane, enabling easier segmentation. In this paper, we create a vessel-segmentation-friendly space using a clinical visualization technique called maximum intensity projection (MIP).