Integrative blood transcriptomic and metabolomic profiling reveals biomarkers of natural heat tolerance in Holstein cows.

Heat stress poses a major threat to dairy cattle productivity, particularly in high-producing Holstein cows. To identify robust biomarkers of thermotolerance, we employed an integrative strategy combining physiological phenotyping, blood metabolite profiling, and transcriptomic analysis. A total of 120 lactating Holstein cows were evaluated under natural summer heat conditions using rectal temperature, respiratory rate, salivation index, serum HSP70, cortisol, potassium levels, and milk production.

Nano-enabled repurposing of Benproperine phosphate enhances pancreatic Cancer chemotherapy through lethal autophagy arrest and immune activation.

Gemcitabine-based chemotherapy remains a cornerstone in pancreatic cancer treatment, yet its efficacy is hindered by poor bioavailability and adaptive resistance mechanisms, such as autophagy. In this study, we developed a hyaluronic acid (HA) modified zeolitic imidazolate framework-8 (ZIF-8) drug-repurposing nanoplatform (HA/ZIF-8@BPP/Gem) against pancreatic cancer through the co-delivery of the antitussive benproperine phosphate (BPP) and gemcitabine (Gem). Using cell lines, patient-derived xenograft models, and orthotopic tumor models, we demonstrated that BPP and Gem, rapidly released from the nanoplatform in the acidic tumor microenvironment, exhibited synergistic cytotoxicity without causing significant biochemical abnormalities or organ toxicity.

Enhancing Catalysis and Sieving Polysulfides by Oxygen Vacancy-Rich Separator Modifier for High-Performance Li-S Batteries.

Lithium-sulfur (Li-S) batteries have been hindered by the low active material utilization, sluggish kinetics and Li anode corrosion caused by the dissolution of Li polysulfides (LiPSs) intermediates. To solve these problems simultaneously, Fe-doped HTiO anchored on the reduced graphene oxide (rGO/HTiO-Fe) is designed via dopant selection and doping content optimization as a novel separator modifier. Tunable Fe-doping induces the controllable generation of oxygen vacancies (OVs), endowing rGO/HTiO-Fe with suitable Lewis acid character, which causes a sieving effect via the differentiated adsorption between OVs and various LiPSs.

Factors Influencing Hormone Remission in Growth Hormone-Secreting Pituitary Neuroendocrine Tumors With Residual Tumor: A Retrospective Cohort Study.

Background: Growth hormone-secreting pituitary neuroendocrine tumors (GH-secreting PitNETs) pose significant health risks due to hormone-related complications. Despite transsphenoidal surgical resection being the primary treatment, complete removal is often infeasible due to invasive growth patterns, leading to postoperative tumor residuals and uncertain hormone remission outcomes.

Methods: This retrospective study included 458 patients with GH-secreting PitNETs who underwent surgery at Beijing Tiantan Hospital.

Quick Determination of Fourteen Rare Earth Elements in Powdered Refractory Materials Using Electrothermal Vaporization with Detection by Inductively Coupled Plasma Optical Emission Spectrometry.

The determination of rare earth elements (REEs) in refractory materials by inductively coupled plasma (ICP) spectrometry with conventional sample introduction using pneumatic nebulization requires the dissolution of these samples, which is difficult. This work presents an optimized method using electrothermal vaporization (ETV) coupled to ICP optical emission spectrometry (OES) for the direct determination of REEs in refractory geological materials. Solid sampling eliminates the dissolution step, thereby increasing sample throughput.

Association between anxiety disorder, anxiolytic drugs, and risk of incident Parkinson's disease.

In this prospective cohort study, we analysed data from 502,364 participants (ages 40-69) in the UK Biobank, with follow-up until 2024. Logistic and Cox regression analysis identified generalized anxiety disorder (GAD) and obsessive-compulsive disorder (OCD) as independent risk factors for Parkinson's disease (PD), with post-traumatic stress disorder (PTSD) patients under 71 also at increased risk. Panic disorder (PAD) showed no association with PD.

NF-κB signalling pathway in neurodegenerative diseases: Acupuncture as a potential therapeutic approach.

The NF-κB signaling pathway plays a crucial role in the pathogenesis of neurodegenerative diseases, including Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis, particularly through its role in the regulation neuroinflammation, oxidative stress, protein misfolding, and apoptosis. Emerging evidence suggests that acupuncture modulates the NF-κB pathway, thus offering therapeutic potential by mitigating neuroinflammation, reducing oxidative stress, and protecting mitochondrial function. Specifically, acupuncture inhibits NF-κB activation, downregulates pro-inflammatory mediators like TNF-α and IL-6, and mitigates neurotoxicity and apoptosis.

The Predictive Value of Real-World Cardiologist Performance (RWCP) Score in Atrial Fibrillation Recurrence Risk After Radiofrequency Ablation.

Objective: To explore the predictive value of Real-world Cardiologist Performance (RWCP) score for atrial fibrillation (AF) recurrence risk after radiofrequency catheter ablation (RFCA).

Methods: Patients with nonvalvular AF who underwent first-time RFCA at Luoyang Central Hospital affiliated to Zhengzhou University between October 2021 and June 2023 were included in this study. Detailed ablation data were exported from CARTO®3 system (Biosense Webster, USA), based on which, four parameters, including discharge time proportion, Catheter Contact Force Stability (C3-FOT proportion), Ablation Index Consistency (SURPOINT/TOP-4 proportion) and fragmented points proportion, were calculated.

Liver metastases in thyroid cancer: epidemiology, risk stratification and survival outcomes in the immunotherapy era.

Purpose: Liver metastases in thyroid cancer are rare but fatal, with poorly defined risk profiles and survival outcomes. This study aimed to characterize epidemiology, risk factors and outcomes of this disease using a population-based approach, further explore the potential impact of the immunotherapy era on the prognosis of these patients.

Methods: Data on 116,801 thyroid cancer cases from SEER program (2010-2021) were analyzed.

MCP-1-CCR2-M2 macrophages axis contributes to diffuse large B-cell lymphoma progression and inhibits antitumor immune response.

Diffuse large B-cell lymphoma (DLBCL) is an aggressive hematological malignancy with restricted effective therapy choices. Neither the recruitment of monocytes nor the functioning of the physiological mechanisms of macrophage polarization can be achieved without the involvement of the MCP-1/CCR2 axis. We investigated the feasibility of treatment targeting MCP-1-CCR2-macrophages axis in DLBCL.

Utilization of Whole Slide Pathological Images and Tumor Clinical Data: A Hybrid Graph Convolutional Network Model for Hepatocellular Carcinoma Prognosis Prediction.

Hepatocellular carcinoma (HCC) is one of the most common cancers worldwide, ranking fourth in cancer-related mortality. Prognostic risk prediction for HCC patients can optimize treatment strategies, assess therapeutic efficacy, and ultimately improve post-operative survival rates. Pathological slides are considered the gold standard for cancer diagnosis and prognosis, playing a crucial role in prognostic risk stratification.

Dual Mechanisms for Heterogeneous Responses of Inspiratory Neurons to Noradrenergic Modulation.

Unlabelled: Respiration is an essential involuntary function necessary for survival. This poses a challenge for the control of breathing. The preBötzinger complex (preBötC) is a heterogeneous neuronal network responsible for driving the inspiratory rhythm.

Emerging Applications of Feature Selection in Osteoporosis Research: From Biomarker Discovery to Clinical Decision Support.

Osteoporosis (OP), a systemic skeletal disease characterized by compromised bone strength and elevated fracture susceptibility, represents a growing global health challenge that necessitates early detection and accurate risk stratification. With the exponential growth of multidimensional biomedical data in OP research, feature selection has become an indispensable machine learning paradigm that improves model generalizability. At the same time, it preserves clinical interpretability and enhances predictive accuracy.

Dual Mechanisms for Heterogeneous Responses of Inspiratory Neurons to Noradrenergic Modulation.

Respiration is an essential involuntary function necessary for survival. This poses a challenge for the control of breathing. The preB\"otzinger complex (preB\"otC) is a heterogeneous neuronal network responsible for driving the inspiratory rhythm.

Impact of the Gut Microbiota-Metabolite Axis on Intestinal Fatty Acid Absorption in Huainan Pigs.

The gut microbiota critically influences lipid metabolism and fat deposition in pigs, processes that underpin pork quality preferences and differentiate the meat traits of Chinese indigenous breeds (fat-type) from those of Western commercial breeds (lean-type). To explore the mechanisms underlying breed-specific fatty acid absorption, we compared the rectal and colonic microbiota and metabolite profiles of Huainan and Large White pigs using 16S rRNA sequencing and untargeted metabolomics. HN pigs exhibited enriched and , along with a significantly higher Firmicutes/Bacteroidetes ratio.

Use of extracorporeal membrane oxygenation in adult trauma patients with refractory acute cardiopulmonary failure: guideline from the Chinese society of extracorporeal life support 2025.

Background: Adult trauma patients with refractory acute cardiopulmonary failure suffer from high morbidity and mortality. In the past decade, a growing body of researches has shown survival benefits of extracorporeal membrane oxygenation (ECMO) in trauma patients who fail to respond to optimal damage control resuscitation (DCR), and there is an opportunity to formulate clinical practice guidelines to guide clinicians in implementing trauma ECMO at the bedside.

Methods: The Chinese Society of Extracorporeal Life Support (CSECLS) convened a domestic panel of interdisciplinary experts to develop this guideline, adhering to the principles of the World Health Organization (WHO) Manual for Guideline Development and the policy of conflict of interest.

Serum ferritin and risk of stroke: a meta-analysis of observation studies.

Background And Purpose: Serum ferritin, a well-established biomarker of iron status, has been inconsistently linked to stroke risk in previous studies. This meta-analysis aims to systematically evaluate the association between serum ferritin levels and the risk of stroke.

Methods: We conducted a systematic literature search across the PubMed and Embase databases to identify relevant studies.

Grass carp reovirus VP6 inhibits IFN production by degrading TBK1 through the autophagy pathway.

The interferon (IFN) response serves as a powerful defense mechanism against viral infections in fish. However, grass carp reovirus (GCRV) is capable of evading the IFN system, although the specific mechanisms remain unclear. In this study, we report that GCRV VP6 employs an immune evasion strategy by degrading TANK-binding kinase 1 (TBK1) through the autophagy pathway, thereby inhibiting IFN activation.

Integrating Body Composition and Nutritional Indices: A Novel Prognostic Tool for Survival in Pancreatic Cancer.

Background: Sarcopenia and malnutrition have been independently associated with a poorer prognosis in pancreatic ductal adenocarcinoma (PDAC), but their combined association with patient outcomes is not fully understood. This study aimed to systematically evaluate the synergistic effects of body composition parameters and nutritional index as prognostic indicators in patients with PDAC.

Methods: A total of 596 patients with PDAC who underwent surgical resection from two centres were initially enrolled in this retrospective study.

High frequency electrical stimulation entrains fast spiking interneurons and bidirectionally modulates information processing.

Background: Clinical intracranial electrical stimulation often deploys trains of high frequency pulses. While brief bursts of stimulation are known to heterogeneously modulate neuronal spiking, it is unclear how trains of high frequency pulses influence neural dynamics.

Objective: As fast spiking interneurons (FSIs) can support rapid firing, we seek to determine how high frequency stimulation modulates FSIs.

Nested Case-Control Study of Intervention in Early Infancy to Prevent Tuberous Sclerosis Complex-Associated Renal Angiomyolipoma.

Introduction: In current studies, treatment of tuberous sclerosis complex (TSC)-related renal angiomyolipoma (RAML) was initiated only after clinical progression or the onset of symptoms, rather than at an earlier stage in the disease process. However, the previous case report indicated that early mammalian target of rapamycin (mTOR) inhibition in patients with TSC might prevent the development of TSC lesions. We performed this nested case-control study to evaluate whether prophylactic sirolimus initiation in early infancy prevented TSC-related RAML (TSC-RAML).

Creating free standing covalent organic framework membranes by nanocrystal suturing in sol gel solutions.

The sol-gel synthesis represents a versatile platform to fabricate ceramic inorganic membranes. However, it is still a grand challenge to push the boundary of sol-gel chemistry towards high-quality organic membrane construction. Herein, a facile and controlled nanocrystal suturing strategy in sol-gel solutions is developed to afford highly crystalline and free-standing covalent organic framework membranes.

Dielectric properties of zwitterionic liquids.

The dielectric properties of a series of zwitterionic liquids are thoroughly examined in both the solution and melt states. In dilute solutions, the molar dielectric increment and subsequently derived dipole moment of zwitterionic liquids correlate strongly with the number of linker atoms between the ion pair, following the same trend as previously extensively studied zwitterionic solids. Further analysis indicates that the linker does not adopt a fully extended conformation but is slightly coiled.

Comprehensive review of Bayesian network applications in gastrointestinal cancers.

Gastrointestinal cancers, including esophageal, gastric, colorectal, liver, gallbladder, cholangiocarcinoma, and pancreatic cancers, pose a significant global health challenge due to their high mortality rates and poor prognosis, particularly when diagnosed at advanced stages. These malignancies, characterized by diverse clinical presentations and etiologies, require innovative approaches for improved management. Bayesian networks (BN) have emerged as a powerful tool in this field, offering the ability to manage uncertainty, integrate heterogeneous data sources, and support clinical decision-making.

Analysis of and methods for void-free liquid filling of blind microchambers in centrifugal microfluidics.

Centrifugal microfluidics are widely used in point-of-care testing applications. Blind microchambers, microchambers that have only one access point by which to interact with an external environment, are commonly used in centrifugal microfluidic chips. However, achieving void-free liquid filling of blind microchambers poses a significant challenge as the injection of liquid and the exhausting of air occurs simultaneously and thus interference leads to incomplete liquid filling with the presence of residual bubbles.