Association between accelerometer-measured physical activity volume and sleep duration in older adults: a cross-sectional interpretable machine learning analysis.

Objective: This study aimed to examine the relationship between physical activity volume and sleep duration in older adults, using objective monitoring data to investigate their non-linear association and threshold effects, thereby providing references for developing exercise programs to improve sleep duration.

Methods: The study used two consecutive waves of NHANES cross-sectional data (2011-2014) as the derivation cohort and NHANES 2005-2006 data as the validation cohort. Analysis of the derivation cohort included weighted univariate analysis, weighted multivariate logistic regression, and interpretable machine learning analysis.

Substrate recycling amplification enabled by hierarchically mesoporous MOFs for the specific screening of pheochromocytoma and paraganglioma.

The sensitive and specific diagnosis of pheochromocytoma and paraganglioma (PPGL) remains challenging due to the low abundance of their detectable biomarker of catecholamines (CAs) in serum. Herein, we established an enzyme-assisted substrate recycling amplification model based on a hierarchically mesoporous UiO-66-NH (HMAUiO) to address these difficulties. Such porous platform was constructed by the Hofmeister ion-assisted nanoemulsion method with mesoporous size beyond 20 nm, in which Pt (II) meso-tetra(4-carboxyphenyl)porphyrin (TCPP-Pt) was incorporated within the microporous framework to form an HMAUiO-Pt sensing probe.

Functional characteristics of fresh antitumor immune interferer GDF-15 in multiple cancers.

We designed our study with the aim of conducting a comprehensive analysis of the role that GDF-15 plays in pan-cancer. Multiple databases were used to obtain GDF-15 expression. XIANTAO Academic and Sangerbox were utilized to conduct the diagnostic significance of GDF-15 expression across pan-cancer.

Barriers and facilitators to addressing sedentary behaviour and physical inactivity among nursing home residents: a qualitative study.

Background: Sedentary behaviour and physical inactivity contribute to muscle-related decline (e.g., sarcopenia), chronic disease and disability.

Abrupt weakening of deep Atlantic circulation at the last glacial inception.

Deglaciations and glacial inceptions are the two equally important transitional periods that bridge the glacial and interglacial climate states, yet our understanding of deglaciations far exceeds that of glacial inceptions. Substantial variations in deep ocean circulation accompanied the last deglaciation, and model simulations recently suggested that a weakening of the Atlantic Meridional Overturning Circulation (AMOC) also occurred at the last glacial inception (LGI; 113-119 thousand years ago), yet evidence of such a change remains inconclusive. Here, we report three Pa/Th records from the western and central North Atlantic that display an abrupt weakening of the AMOC at the LGI.

Factors influencing engagement with patient-directed and facilitated advance care planning interventions for patients with advanced cancer.

Background: Advance care planning (ACP) for patients with cancer is guideline recommended. However, there are significant challenges to engagement. Patient-facing ACP programs have been developed to improve access and engagement.

Ecological factors impacting genetic characteristics and metabolite accumulations of .

Objective: The investigation of the correlation between ecological factors and the genetic characteristics or metabolites of plants offers valuable insights into the regional causes of genetic and metabolic diversity. Here, , a medicinal plant, is employed as a model to explore the environmental factors that influence its genetic characteristics and metabolic accumulations.

Methods: A total of 23 populations from six cultispecies and 11 cultivated regions were selected based on the predictions of the global geographic information system.

miRNA rco-MIR395a-p5_1ss8TC suppresses CsOMT957 expression leading to reduction in cannflavin A accumulation in Cannabis sativa L.

Cannflavin A is a unique flavonoid in Cannabis sativa. To date, the mechanism of miRNA in regulating cannflavin A biosynthesis in C. sativa is not clear.

Preparation, characterization and antioxidative activity analysis of recombinant humanized collagen type III-loaded liposomes.

To improve the bioavailability and biological activity of recombinant type III humanized collagen (rhCol III), we optimized the preparation process of recombinant type III humanized collagen-loaded liposomes (rhCol III-LIPS) using the film dispersion and periplasmic extrusion methods, with the encapsulation rate as the response value. Then we analyzed the antioxidative activity of rhCol III-LIPS by determining the scavenging rate of free radicals. We investigated the protective effect of rhCol III-LIPS against HO-induced injuries in HaCaT cells by detecting cell vitality, contents of reactive oxygen species (ROS), malondialdehyde (MDA), and activities of antioxidases superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase dismutase (GSH-Px).

Corilagin regulates antigen processing and presentation by directly binding to inhibit ERAP1.

ERAP1 is a critical aminopeptidase that acts as the final "editor" of endogenous antigen peptides in the endoplasmic reticulum, playing a key role in the MHC-I-mediated antigen processing and presentation pathway. It has become a novel target for a broad class of severe autoimmune diseases, referred to as "MHC-I-opathy," as well as for tumor immune evasion. Here, we report the discovery of a food-derived natural product inhibitor, Corilagin, with a novel scaffold that directly interacts with ERAP1, identified through high-throughput screening.

-Alkylation of Amines with 2,5-Furandimethanol to ,'-Disubstituted 2,5-Bis(aminomethyl)furans Catalyzed by a Metal-Ligand Bifunctional Iridium Catalyst.

A general method for the -alkylation of amines with 2,5-furandimethanol via hydrogen autotransfer process has been accompanied. In the presence of [Cp*Ir(2,2'-bpyO)(HO)] (0.5-1 mol %) and CsCO (0.

High frequency electrical stimulation entrains fast spiking interneurons and bidirectionally modulates information processing.

Background: Clinical intracranial electrical stimulation often deploys trains of high frequency pulses. While brief bursts of stimulation are known to heterogeneously modulate neuronal spiking, it is unclear how trains of high frequency pulses influence neural dynamics.

Objective: As fast spiking interneurons (FSIs) can support rapid firing, we seek to determine how high frequency stimulation modulates FSIs.

Clinical presentation of PD-1/PD-L1 immune checkpoint inhibitors induced cystitis.

This study aims to systematically analyze the clinical features of cystitis induced by anti-PD-1/PD-L1 drugs, with a view to providing a basis for early warning, diagnosis, and standardized management of this adverse reaction in clinical practice. In this study, we comprehensively searched multiple databases to systematically collect all case reports involving cystitis induced by anti-PD-1/PD-L1 drugs from the initiation of relevant research up to May 20, 2025, and conducted an in-depth retrospective analysis of these cases. The clinical characteristics of 43 patients with cystitis induced by anti-PD-1/PD-L1 agents in this study showed a median age of 57 years (27-78 years), with 26 cases (60.

Effectiveness of the Family-Centred Empowerment Model on Informal Caregivers of People With Chronic Diseases: A Systematic Review and Meta-Analysis.

Aims And Objectives: To investigate the effects of the Family-Centred Empowerment Model on informal caregivers on individuals with chronic diseases.

Background: The Family-Centred Empowerment Model may help address informal caregivers' challenges in chronic disease management, including caregiver burden. However, its effectiveness for informal caregivers of chronic disease patients remains unclear.

PT-SRiApt Targeting SCAF4-POLR2A Interaction Suppresses Tumor Growth and Promotes Antitumor Immunity in Triple-Negative Breast Cancer.

The interaction between SCAF4 and RNA polymerase II (POLR2A) is crucial for proper mRNA termination, with its dysregulation leading to truncated mRNAs and nonfunctional proteins, impairing cellular growth. Despite its potential relevance, the role of this interaction in triple-negative breast cancer (TNBC) remains unexplored due to the lack of effective molecular tools. To address this, we employed SRiApt, an aptamer generated through the recently established Blocker-SELEX pipeline.

Ultralow-Temperature Carboxylate Electrolyte for High-Voltage Lithium Metal Batteries.

Carbonate-based electrolytes possess high oxidative stability and solvation ability to Li in Li metal batteries (LMBs). However, they face significant challenges under cryogenic temperatures, including the sluggish reaction kinetics, uneven Li deposition, and severe interfacial side reactions, especially under the elevated cutoff voltages. Carboxylates usually have lower viscosity and freezing points.

Zika virus non-structural protein NS2A mediated endoplasmic reticulum stress through interacting with Sarco/endoplasmic reticulum Ca-ATPase 2.

Unlabelled: Zika virus (ZIKV) infection of neuronal cells leads to endoplasmic reticulum (ER) stress, which is one of the key causes of neuronal damage. However, how ZIKV mediates ER stress has not been fully understood. Here, we observed that ZIKV infection of astrocytes elevated Sarco/endoplasmic reticulum Ca-ATPase (SERCA) expression, increased intracellular Ca concentration, and upregulated ER stress-related genes.

Prolonged Single Neuron Voltage Imaging in Behaving Mammals.

Long-term recording of single-neuron membrane voltage dynamics during behavior is highly desirable. Using ElectraOFF, a fully genetically encoded, photostable, high-performance fluorescence voltage indicator, we achieve routine cellular-resolution imaging over tens of minutes, occasionally up to eighty minutes, in behaving mice with minimal signal loss across neuron types and brain regions. This extended recording capability reveals plasticity changes throughout intracranial electrical neuromodulation, highlighting the novel insights enabled by prolonged voltage imaging.

Self-assembled copper chlorogenic acid nanoparticles: Inducing pyroptosis and cuproptosis to activate antitumor immunity.

Currently, the tumor treatment faces many challenges. Although chemotherapy is the most effective and economical choice, most chemotherapy drugs lack tumor specificity, have low bioavailability, and face many obstacles in clinical application. Significantly, the tumor immunosuppressive microenvironment (TIME) and low immunogenicity in solid tumors also seriously affect the clinical effect of tumor therapy.

Unraveling the Efficacy of AR Antagonists Bearing -(4-(Benzyloxy)phenyl)piperidine-1-sulfonamide Scaffold in Prostate Cancer Therapy by Targeting LBP Mutations.

Point mutations in the androgen receptor (AR) are significant drivers of resistance in prostate cancer (PCa), posing a great challenge to the development of effective treatment strategies. Building on our previous discovery of the suboptimal AR antagonist , we developed , which contains an -(4-(benzyloxy)phenyl)piperidine-1-sulfonamide scaffold through structural optimization and comprehensive screening against T878A-mutated AR. outperformed existing antiandrogens by fully antagonizing clinical AR mutations and effectively suppressing castration- and enzalutamide-resistant LNCaP cells proliferation .

Positive Psychology Insights on the Effects of Spirituality on Shared Decision-Making in Patients with Chronic Heart Failure: The Chain-Mediated Effects of Benefit-Finding and Decision Self-Efficacy.

: As the terminal stage of cardiovascular disease, heart failure (HF) has garnered significant attention due to its recurrent nature, high mortality rates, and substantial medical burden. Shared decision-making (SDM) is an innovative strategy to improve medication adherence. From positive psychology insights, the effects on spirituality, benefit-finding (BF), decision self-efficacy, and patient engagement in SDM remain unexplored.

Evaluation of bacterial endophytes from turfgrass for effective biocontrol of dollar spot disease.

Background: Clarireedia spp. are a group of highly destructive phytopathogenic fungi that affect both cool- and warm-season turfgrasses, leading to the development of dollar spot (DS) disease. Biological control presents a promising alternative to chemical methods, offering environmental benefits and eliminating the risk of fungicide resistance development.

Enhancing readiness for advance care planning among community-dwelling older adults with frailty: A mixed-method systematic review.

Background: Given uncertain illness trajectories faced by older adults with frailty and risk of decline and poor outcomes, intervention development for readiness to engage with advance care planning may confer benefits. However, evidence in this area remains limited.

Aim: DESIGN: A mixed-methods systematic narrative review, underpinned by the COM-B system behaviour change model and employing thematic data synthesis.

The mechanisms of self-inhibited reactions during hydroxyl radical-induced degradation of aniline disinfection by products.

Municipal wastewater post-treatment processes can effectively reduce the concentration of highly toxic disinfection by-products (DBPs) to minimize their ecological hazards. In this study, the reaction mechanisms and kinetics of 38 aniline disinfection by-products (AN-DBPs) degraded by ·OH in the post-treatment of municipal wastewater were investigated. Results showed that the apparent second-order reaction rate constants for the degradation of AN-DBPs by ·OH ranged from 1.

Anionic Fries Rearrangement of -Iodophenyl Carboxylate Initiated by Potassium Hydride.

In order to generate an aryl anion, metal-halogen exchanges initiated by alkyllithium, lithium amide, or Grignard reagent are frequently employed. However, carbonyl groups are not compatible with these reagents. Herein, we demonstrate that aryl iodide can undergo direct K-I exchange with KH to produce an aryl anion species.