Lipid Metabolism and Immune Crosstalk in Fish Gut-Liver Axis: Insights from SOCS8 Knockout and Dietary Stress Models.

Metaflammation, a chronic immune response triggered by metabolic dysregulation, poses significant threats to gut-liver homeostasis in aquaculture species. To understand the progression of metaflammation, it is crucial to examine the role of SOCS8 deficiency in socs8 zebrafish, as this species may serve as a disease model for metabolic disorders due to the gradual dysregulation of immunity, metabolism, and the gut microbiota observed in them. This study examines the immune-metabolic crosstalk in grass carp, subjected to soybean meal-induced enteritis, and in socs8 zebrafish under genetic and dietary stress.

Controlled synthesis of Zn-doped MnO nanoplates for MRI-guided metal ion interference synergistic therapy for breast cancer.

Manganese dioxide (MnO) nanomaterials have emerged as a promising class of nanoplatform for the therapeutic management of tumors due to their regulable physicochemical properties and good biocompatibility. However, the rational design of MnO nanomaterials often decreased the therapeutic efficacy of tumors due to the inherent protective mechanisms of eliminating the imbalance of Mn in cells. Herein, we firstly prepared a novel Zn ion doped MnO nanoplate (Zn-MnO) employing different zeolitic imidazolate framework 8 (ZIF8) precursors as Zn ion source, which possessed enhanced T1-weighted magnetic resonance imaging (MRI) signal, reactive oxygen species (ROS) generation capacity and efficient photothermal conversion.

Curcumin derivatives as ferroptosis inducers: design, synthesis and anti breast cancer activity evaluation.

Curcumin (CUR) is a natural product isolated from Curcuma longa L., which has become a research hotspot due to its significant anti-tumor activity by inducing tumor cell apoptosis and inhibiting proliferation. This study designed and synthesized 20 CUR derivatives containing 1,2,3-triazoles and heterocycles by modifying the methylene group between two carboxyl groups of CUR and hybridizing with aldehyde containing fragments.

Stroke risk in women with or without hysterectomy and/or bilateral oophorectomy: evidence from the NHANES 1999-2018 and meta-analysis.

Objective: We aimed to assess the relationship between hysterectomy and/or bilateral oophorectomy and the risk of stroke-a topic of ongoing debate in current research.

Methods: We utilized data from the National Health and Nutrition Examination Survey (NHANES) 1999-2018 to estimate both crude and multivariable-adjusted hazard ratios (HRs) and 95% CIs, applying survey-weighted Cox proportional hazards regression model. The modeling incorporated sampling weights and design variables to address NHANES's multistage probability sampling framework.

Cold Inducible RNA-Binding Protein: A Novel Therapeutic Target for Ventricular Arrhythmia Control.

Ventricular arrhythmias following myocardial infarction (MI) remain a leading cause of sudden cardiac death, yet therapeutic options are limited by incomplete understanding of the molecular mechanisms governing post-infarction arrhythmogenesis. While RNA-binding proteins have emerged as critical regulators of cardiovascular pathophysiology, their role in cardiac electrophysiology remains largely unexplored. Here, we demonstrate that cold- inducible RNA-binding protein (CIRP) functions as a critical regulator of ventricular arrhythmia susceptibility through post-transcriptional control of cardiac ion channels.

scUCAF: An uncertainty-aware cross-omics alignment and fusion network for single-cell multi-omics data clustering.

The development of single-cell multi-omics sequencing technologies provides new insights into cell heterogeneity. Cell clustering is a crucial step in the analysis of multi-omics data. However, existing methods often overlook variations in data quality across omics, leading to unreliable feature representations.

(Sangzhi) Alkaloids Improve Pancreatic β-Cell Function Through Gut Microbial and Intra-Islet 2-Methoxyestradiol Biosynthesis.

: (Sangzhi) Alkaloids (SZ-A) are natural hypoglycemic compounds known to enhance insulin secretion. Given the emerging role of the gut microbiota in regulating β-cell function, in this study, we aimed to investigate whether SZ-A exert their beneficial effects through modulating the gut microbiota and its metabolites. : A diabetic mouse model was established using a high-fat diet and streptozotocin, followed by 20 weeks of SZ-A treatment.

Endovascular thrombectomy combined with decompressive craniectomy for malignant anterior circulation large vessel occlusion stroke:A real-world study.

Objective: To evaluate the efficacy of endovascular thrombectomy (EVT) combined with decompressive craniectomy (DC) in patients with malignant anterior circulation large vessel occlusion (MACLVO) stroke.

Methods: We retrospectively analyzed clinical data from 189 MACLVO stroke patients who received EVT with or without DC. Outcomes included onset to treatment (OTT) time, NIHSS score, ASPECTS scores, NICU stay, and 90-day mRS score.

Flexible wearable sensors based on multifunctional carboxymethylcellulose/polyacrylamide/gelatin hydrogels: Collaborative design of high-sensitivity monitoring and visual feedback.

Creating flexible hydrogels that combine high mechanical strength, conductivity, and functionality remains a challenge. Sodium carboxymethyl cellulose (CMC) was successfully added to a double network composite hydrogel based on polyacrylamide (PAM)/gelatin, resulting in a synergistic improvement in visualization, excellent mechanical properties (stress = 0.458 MPa, strain = 946 %, toughness = 1.

Dysfunctional circular RNA network in major depressive disorder: dissecting the cell identity and potential clinical applications.

Circular RNAs (circRNAs), characterized by their unique covalently closed circular structure, are highly stable, conserved, and expressed in a tissue-, cell-, and developmental stage-specific manner. A lot of evidence has indicated that circRNAs play a crucial role in diversified biological and pathological processes by sponging microRNAs (miRNAs), interacting with proteins, or being translated into particular proteins, etc. Recent advancements in bioinformatics have revealed a close association between circRNAs and the development of neuropsychiatric diseases, including major depressive disorder (MDD).

To Evaluate the Feasibility of Exergames for Fine Motor Skill Rehabilitation Among Acute Stroke Patients in a Tertiary Hospital: A Quasi-experimental Study.

Upper limb (UL) impairments can affect fine motor control, including the ability to grasp and manipulate objects. Exergames, an innovative and technology-driven intervention, may enhance patient engagement and adherence to rehabilitation exercises. However, there is limited evidence assessing their feasibility in the acute care setting, immediate post-stroke.

Ramulus Mori (Sangzhi) alkaloids enhance pancreatic β-Cell maturation and function by targeting the CD81/endoplasmic reticulum stress pathway.

Background: β-cell dedifferentiation and impaired maturation are crucial contributors to β-cell dysfunction in type 2 diabetes mellitus (T2DM), yet targeted therapies remain limited.

Objective: To investigate the protective effects of Ramulus Mori (Sangzhi) Alkaloids (SZ-A) on β-cell function and maturation in T2DM.

Methods: A T2DM mouse model was induced by high-fat diet and streptozotocin, followed by SZ-A treatment.

Prognostic significance of micropapillary pattern and risk factors in patients with resected stage I lung adenocarcinoma and possible benefit of adjuvant therapy: a real-world multicenter study.

Background: Micropapillary (MP) pattern has been identified as a negative prognostic factor in patients with lung adenocarcinoma, but it has not been recognized as a high-risk factor for patients with stage IB lung adenocarcinoma treated with adjuvant chemotherapy. This multicenter cohort study aimed to evaluate the prognostic value of histological subtypes for stage I lung adenocarcinoma and to determine the relative survival benefit of adjuvant chemotherapy for subgroups based on MP pattern.

Methods: This retrospective study included 412 patients with stage I lung adenocarcinoma [according the eighth edition of the tumor-node-metastasis (TNM) classification] with MP pattern who underwent complete surgical resection between January 2010 and December 2019.

Gut microbiota alterations modulate high-fat diet-induced precocious puberty.

The link between gut microbiota and precocious puberty has garnered increasing attention; however, the underlying mechanisms remain poorly understood, and most existing studies are cross-sectional, making it difficult to capture the longitudinal changes in gut microbiota throughout precocious puberty. Here, female rats were fed high-fat diets supplemented with antibiotics to establish a model of precocious puberty with gut microbiota perturbation. Gut microbiota dynamics were characterized based on stool samples collected at postnatal day (PND) 21, 25, 29, and 33.

Diagnosis and genetic analysis of Gaucher disease in a pediatric case: a case report.

A 2-year-old patient was admitted to our hospital with hepatosplenomegaly as the prominent clinical feature. Peripheral blood analysis during hospitalization revealed trilineage cytopenia. Bone marrow cytology examination demonstrated abundant suspected Gaucher cells.

Extrafoveal processing of happy face relies on visual awareness in hearing-impaired adults.

Hearing loss is the most common sensory deficit. Deaf adults outperform hearing adults in terms of the visual processing of extrafoveal moving stimuli and fearful facial expressions, probably due to compensatory mechanisms. However, it remains unresolved whether these enhanced visual abilities also apply to happy facial expressions, which are the most universally recognized and prioritized prosocial signals among all the facial expressions.

Regulation of cortical neurogenesis by MED13L via transcriptional priming and its implications for MED13L syndrome.

Pathogenic variants in MED13L cause MED13L syndrome, a well-defined neurodevelopmental disorder characterized by motor deficits, intellectual disability, and language delay, yet its underlying developmental and molecular mechanisms remain largely elusive. To address this gap, we generated a gene knockout mouse model of MED13L syndrome. While homozygous Med13l knockout exhibit neonatal lethality accompanied by reduced brain volume and cortical thickness, heterozygous mice are viable and display hallmarks of MED13L syndrome, including impaired learning and memory, reduced motor coordination, and heightened anxiety.

Effect of Acupuncture on Functional Connectivity of the Limbic Network in Patients with Knee Osteoarthritis: A Resting State Functional Magnetic Resonance Imaging Study.

Background: Knee osteoarthritis (KOA) is a prevalent degenerative joint disorder. Acupuncture therapy demonstrates significant efficacy in alleviating KOA symptoms. However, the central neuroimaging mechanisms underlying acupuncture's therapeutic effects remain incompletely elucidated.

Genome-wide study and expression analysis of soybean ERF transcription factors and overexpression of GmERF205 enhances drought resistance in soybean.

Background: Soybean (Glycine max) is an important food crop in China. In recent years, the continuous deterioration of environmental conditions has significantly affected the growth and development of soybean, particularly under drought stress. Ethylene-responsive element binding factor (ERF) transcription factors, which are unique to plants, play crucial roles in various physiological and biochemical processes during plant growth and development.

The Smallest Worthwhile Effect as a Promising Alternative to the MCID in Estimating PROMs for Adult Idiopathic Scoliosis.

Background: The smallest worthwhile effect (SWE) enables patients to evaluate the expected value of a treatment by weighing its benefits, risks, and costs. It has emerged as an alternative to the minimal clinically important difference (MCID) for interpreting patient-reported outcome measures (PROMs). The purposes of this study were to determine the SWE estimates and MCID thresholds in patients undergoing surgery for adult idiopathic scoliosis (AdIS) and to verify whether meeting or exceeding the SWE estimates correlates with satisfaction at a minimum of 2 years postoperatively.

Endometriosis and Mortality Risk in US Women: Findings From NHANES 1999-2006.

Objective: Less is known about the link between mortality and endometriosis, an often chronic, inflammatory gynecologic condition. We assessed the association between endometriosis and all-cause and cause-specific mortality using a retrospective cohort study design based on data from the National Health and Nutrition Examination Survey (NHANES) 1999-2006.

Materials And Methods: Both crude and multivariable-adjusted hazard ratios (HRs) and 95% confidence intervals (CIs) for endometriosis and age at endometriosis with all-cause mortality were estimated using weighted Cox proportional hazards regression.

Triglyceride-glucose index: a novel assessment tool for all-cause mortality in critical stroke patients-a retrospective analysis of the eICU-CRD database.

Background: Stroke, a leading cause of death and disability, includes ischemic (IS), hemorrhagic (HS), and subarachnoid subtypes. While the triglyceride-glucose index (TyG) has been linked to cerebrovascular diseases, studies focus on single subtypes and lack ICU data. The prognostic roles of the atherogenic index of plasma (AIP) and total cholesterol-to-HDL ratio (TC_HDL) in critically ill stroke patients' all-cause mortality (ACM) remain unexplored.

Nitric oxide‑mediated S‑Nitrosylation contributes to signaling transduction in human physiological and pathological status (Review).

In the complex development of various diseases, nitric oxide‑mediated S‑nitrosylation is increasingly recognized for its distinct regulatory function. Recent research has advanced our knowledge of how this nitric oxide‑dependent modification is dynamically controlled under both physiological and pathological conditions. S‑nitrosylation plays a key role in regulating mitochondrial function, gene transcription, cellular homeostasis and metabolism and it is also involved in the pathogenesis of cardiovascular disorders, neurological conditions and cancer.

Icotinib plus chemotherapy as neoadjuvant treatment for resectable stage II-IIIB EGFR-mutant lung adenocarcinoma: a phase II study (NEOIPOWER).

Background: EGFR tyrosine kinase inhibitors (EGFR-TKIs) have shown activity in the neoadjuvant setting of EGFR-mutant (EGFRm) non-small cell lung cancer; however, data on the combination of neoadjuvant EGFR-TKIs plus chemotherapy are limited. This study aimed to evaluate the safety and efficacy of neoadjuvant icotinib plus chemotherapy for stage II-IIIB EGFRm lung adenocarcinoma.

Methods: NEOIPOWER is a single-arm, phase II study (NCT05104788).

Differentiation Trajectory of Virus-Induced Tumour Cells in Rice Revealed by Single-Cell RNA Sequencing.

Many plant viruses trigger abnormal differentiation and development of host cells, causing distinct symptoms. Here, in a single-cell RNA sequence analysis, we find transcriptional heterogeneity between cells from rice leaf sheaths that are infected with rice black streaked dwarf virus (RBSDV) and from those that are virus-free. Using 106 973 cells, we construct a single-cell transcriptome atlas with 12 cell types and find that parenchyma and vascular parenchyma cells are the two major cell types affected by RBSDV.