Genetic Adaptation to Brackish Water and Spawning Season in European Cisco.

How species adapt to diverse environmental conditions is essential for understanding evolution and the maintenance of biodiversity. The European cisco (Coregonus albula) is a salmonid that occurs in both fresh and brackish water, and this together with the presence of sympatric spring- and autumn-spawning lacustrine populations provides an opportunity for studying the genetics of adaptation in relation to salinity and timing of reproduction. Here, we present a high-quality reference genome of the European cisco based on PacBio HiFi long read sequencing and HiC-directed scaffolding.

Proteins derived from Bombyx batryticatus utilizing variable ultrasound powers: Evaluation of structural attributes, functional characteristics, and antioxidant capacities.

The current investigation evaluated the impact of diverse ultrasound power levels (0, 120, 200, 320, 400, and 520 W) on the structural, functional, and antioxidant potentials of proteins derived from Bombyx batryticatus (PBB). Findings indicated a marked increase in the yield of PBB via ultrasonic treatment at the appropriate ultrasound power (UP), accompanied by a diminished β-sheet content and an augmented proportion of α-helix and β-turn conformations. Furthermore, ultrasound extraction (UAE) at the appropriate UP broke down PBB into small and evenly dispersed fragments, and it also reshaped disordered PBB aggregates into a more regular, dispersed, and ordered arrangement.

R2R3-MYB transcription factor CaMYB5 regulates anthocyanin biosynthesis in pepper fruits.

Anthocyanins, are key polyphenolic secondary metabolites, such as delphinidin, cyanidin, rutin, and epicatechin that regulate plant coloration. Purple pepper fruits have high commercial value and industrial application potential due to their rich in accumulation of anthocyanins. This study identified and cloned an R2R3-MYB transcription factor, CaMYB5 from pepper fruits.

Utilizing ultrasound for the extraction of polysaccharides from the tuber of Typhonium giganteum Engl.: Extraction conditions, structural characterization and bioactivities.

Polysaccharides from the dried tuber of Typhonium giganteum Engl. (TGEPs) were obtained by utilizing ultrasonic-assisted extraction (UAE) as the extraction method. The determination of optimal process parameters for the UAE of TGEPs (TGEP-U) was accomplished through the application of response surface methodology (RSM).

Characterization of Thirty Germplasms of Millet Pepper ( L.) in Terms of Fruit Morphology, Capsaicinoids, and Nutritional Components.

Background: Millet peppers have rich and diverse germplasm resources. It is of great significance to characterize their phenotypes and physicochemical indicators.

Methods: 30 millet germplasms were selected to measure the fruit length and width, flesh thickness, number of ventricles, fruit stalk length, and single fruit weight, and the texture characteristics of fruit such as hardness, cohesiveness, springiness, gumminess, and chewiness were determined by a texture analyzer.

Exploring Chinese Secondary School Students' Acceptance of Live Video-Streamed Teaching Platforms in EFL Class: An Application of the Technology Acceptance Model.

Live video-streamed teaching platforms are widely used in language teaching. However, how students perceive these platforms has scarcely been investigated. By adopting the Technology Acceptance Model (TAM), this study investigated Chinese secondary school students' perceptions of the platforms (i.

Limited Parallelism in Genetic Adaptation to Brackish Water Bodies in European Sprat and Atlantic Herring.

The European sprat is a small plankton-feeding clupeid present in the northeastern Atlantic Ocean, in the Mediterranean Sea, and in the brackish Baltic Sea and Black Sea. This species is the target of a major fishery and, therefore, an accurate characterization of its genetic population structure is crucial to delineate proper stock assessments that aid ensuring the fishery's sustainability. Here, we present (i) a draft genome assembly, (ii) pooled whole genome sequencing of 19 population samples covering most of the species' distribution range, and (iii) the design and test of a single nucleotide polymorphism (SNP)-chip resource and use this to validate the population structure inferred from pooled sequencing.

[Study on the total flavonoid content and antioxidant activity of Morus nigra Linn. from different origins in Xinjiang].

Objective: Optimization of the extraction process of total flavonoids from Morus nigra Linn. based on response surface design, to compare the differences in total flavonoid content of Morus nigra Linn. from different origins in Xinjiang and its relationship with antioxidant activity.

Molecular phylogenetic analysis of the East Asian hemicultrine fishes (Teleostei: Cyprinidae: Xenocypridinae), with suggestions to their generic classification and redescription of the recently described species Hemiculter yungaoi Vasil'eva et al. 2022.

The hemicultrine fishes are a group of small-sized cyprinids, widely distributed but endemic to East Asian rivers and lakes. Till now, the taxonomic boundaries and relationships within this group remain poorly explored. In the present study, we study the phylogeny of this group, providing suggestions for classification of the hemicultrine group.

Integrative Metabolome and Transcriptome Analyses Provide Insights into Carotenoid Variation in Different-Colored Peppers.

Carotenoids are important pigments in pepper fruits. The colors of each pepper are mainly determined by the composition and content of carotenoid. The 'ZY' variety, which has yellow fruit, is a natural mutant derived from a branch mutant of 'ZR' with different colors.

Objectification of Tongue Diagnosis in Traditional Medicine, Data Analysis, and Study Application.

Tongue diagnosis is an essential technique of traditional Chinese medicine (TCM) diagnosis, and the need for objectifying tongue images through image processing technology is growing. The present study provides an overview of the progress made in tongue objectification over the past decade and compares segmentation models. Various deep learning models are constructed to verify and compare algorithms using real tongue image sets.

Identification of genetic polymorphisms in unexplained recurrent spontaneous abortion based on whole exome sequencing.

Background: The precise etiology of approximately 50% of patients with recurrent spontaneous abortion (RSA) is unclear, known as unexplained recurrent spontaneous abortion (URSA). This study identified the genetic polymorphisms in patients with URSA.

Methods: Genomic DNA was extracted from 30 couples with URSA and 9 couples with normal reproductive history for whole exome sequencing.

The utility of SARS-CoV-2 nucleocapsid protein in laboratory diagnosis.

Background: The Coronavirus Disease 2019 (COVID-19) is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which has now become a global pandemic owing to its high transmissibility. The SARS-CoV-2 nucleocapsid protein tests are playing an important role in screening and diagnosing patients with COVID-19, and studies about the utility of SARS-CoV-2 nucleocapsid protein tests are increasing now.

Methods: In this review, all the relevant original studies were assessed by searching in electronic databases including Scopus, Pubmed, Embase, and Web of Science.

Hole-filling based on content loss indexed 3D partial convolution network for freehand ultrasound reconstruction.

Background And Objective: During the 3D reconstruction of ultrasound volume from 2D B-scan ultrasound images, holes are usually found in the reconstructed 3D volumes due to the fast scans. This condition will affect the positioning and judgment of the doctor to the lesion. Hence, in this study, we propose to fill the holes by using a novel content loss indexed 3D partial convolution network for 3D freehand ultrasound volume reconstruction.

High Performance of SARS-Cov-2N Protein Antigen Chemiluminescence Immunoassay as Frontline Testing for Acute Phase COVID-19 Diagnosis: A Retrospective Cohort Study.

COVID-19 emerged and rapidly spread throughout the world. Testing strategies focussing on patients with COVID-19 require assays that are high-throughput, low-risk of infection, and with small sample volumes. Antigen surveillance can be used to identify exposure to pathogens and measure acute infections.

Local-global active contour model based on tensor-based representation for 3D ultrasound vessel segmentation.

Three-dimensional (3D) vessel segmentation can provide full spatial information about an anatomic structure to help physicians gain increased understanding of vascular structures, which plays an utmost role in many medical image-processing and analysis applications. The purpose of this paper aims to develop a 3D vessel-segmentation method that can improve segmentation accuracy in 3D ultrasound (US) images. We propose a 3D tensor-based active contour model method for accurate 3D vessel segmentation.

Fragment-based lead discovery of a novel class of small molecule antagonists of neuropeptide B/W receptor subtype 1 (GPR7).

Here, we report the discovery of a new class of NPBWR1 antagonists identified from a fragment-based screen. Compound 1 (cAMP IC = 250 µM; LE = 0.29) emerged as an initial hit.

Can routine laboratory tests discriminate SARS-CoV-2-infected pneumonia from other causes of community-acquired pneumonia?

Background: The clinical presentation of SARS-CoV-2-infected pneumonia (COVID-19) resembles that of other etiologies of community-acquired pneumonia (CAP). We aimed to identify clinical laboratory features to distinguish COVID-19 from CAP.

Methods: We compared the hematological and biochemical features of 84 patients with COVID-19 at hospital admission and 221 patients with CAP.

The presence of SARS-CoV-2 RNA in the feces of COVID-19 patients.

In December 2019, coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), emerged in Wuhan, China, and has spread globally. However, the transmission route of SARS-CoV-2 has not been fully understood. In this study, we aimed to investigate SARS-CoV-2 shedding in the excreta of COVID-19 patients.

Antibodies in Infants Born to Mothers With COVID-19 Pneumonia.

This study describes results of IgM and IgG antibody testing from throat swabs of newborns born to mothers with COVID-19 pneumonia.

Clinical characteristics of severe acute respiratory syndrome coronavirus 2 reactivation.

Objectives: Previous studies on the pneumonia outbreak caused by the 2019 novel coronavirus disease (COVID-19) were based on information from the general population. However, limited data was available for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reactivation. This study aimed to evaluate the clinical characteristics of the SARS-CoV-2 reactivation.

Conservative interventions for treating functional daytime urinary incontinence in children.

Background: In children, functional daytime urinary incontinence is the term used to describe any leakage of urine while awake that is not the result of a known underlying neurological or congenital anatomic cause (such as conditions or injuries that affect the nerves that control the bladder or problems with the way the urinary system is formed). It can result in practical difficulties for both the child and their family and can have detrimental effects on a child's well-being, education and social engagement.

Objectives: To assess the effects of conservative interventions for treating functional daytime urinary incontinence in children.

Quantitative assessment of aberrant methylation in ovarian cancer: a meta-analysis based on literature and TCGA datasets.

Epigenetic alteration of is conventionally thought to induce the initiation of carcinoma. However, the role of methylation in ovarian cancer still remains controversial. Therefore, we performed a meta-analysis to further elucidate the relationship between promoter methylation and ovarian cancer.

The roles of ncRNAs in the diagnosis, prognosis and clinicopathological features of breast cancer: a systematic review and meta-analysis.

Background: A number of studies have shown that noncoding RNAs (ncRNAs) are abnormally expressed in breast cancers. However, the roles of ncRNAs remain unclear in breast cancer. Here, we aim to investigate the potential diagnostic and prognostic roles of ncRNAs in breast cancer.