A single fluorescent probe for simultaneous detection of polarity, viscosity, and esterase.

Background: The dynamic interplay between esterase activity and physicochemical microenvironments-such as polarity and viscosity-is critical for decoding early cellular dysfunction in processes like apoptosis, ferroptosis, and drug-induced toxicity. However, conventional probes typically report only a single parameter, obscuring interdependent changes in enzyme activity and membrane properties. This technological gap limits our ability to capture real-time, spatially resolved fluctuations within subcellular compartments.

Combined use of 3D-printed customized prostheses and denosumab for the treatment of giant cell tumor of the distal radius: clinical outcomes and efficacy.

Objective: To investigate the short- and mid-term clinical efficacy of denosumab combined with 3D-printed prosthesis in the treatment of patients with giant cell tumor of the distal radius.

Methods: From January 2016 to January 2022, 20 patients with giant cell tumor of the distal radius underwent denosumab treatment combined with 3D-printed prosthetic reconstruction at our hospital. This study evaluates the short- and mid-term efficacy by analyzing clinical cases where denosumab was used preoperatively, followed by 3D-printed biological prosthesis reconstruction of the distal radius tumor segment defect.

Sirtuin 6 mitigates thoracic aortic aneurysm progression via maintenance of mitochondria homeostasis in vascular smooth muscle cells.

Progressive loss of vascular smooth muscle cells (VSMCs) is the pathophysiological basis for aortic aneurysm and dissection (AAD), a life-threatening disease, but the underlying mechanisms are largely unknown. Sirtuin 6 (SIRT6), a class III histone deacetylase, is critical for maintenance of VSMC homeostasis and prevention of vascular remodeling-related diseases. In this study, we investigated the role of VSMC SIRT6 in AAD and the molecular mechanism.

Comparative Evaluation of Stress Distribution and Permeability Characteristics in Bentonite Cutoff Walls Using CPTU and ABAQUS Methods.

Bentonite materials are extensively used in cutoff walls at landfill sites. This study calculates the stress and permeability characteristics of bentonite materials using the piezocone penetration test (CPTU) and ABAQUS simulations. The lateral effective stress of bentonite materials is evaluated using arching models, lateral squeezing models, and a modified lateral squeezing model.

Dynamic construction of a durable epitaxial catalytic layer for industrial alkaline water splitting.

Optimizing the catalyst-electrolyte interface structure is crucial for enhancing the performance of electrochemical alkaline hydrogen evolution reaction. Traditional approaches typically focus on regulating the thermodynamic barriers of adsorption and desorption for reactants, intermediates, and ions at active sites on the solid electrode surface. However, the structure of the electrical double layer influences the concentration of intermediates, adsorption energy, and surface reaction kinetics.

Transferring asymmetrical [2Fe2S] clusters from the mitochondrial protein MiNT to acceptor.

NEET protein is an evolutionarily conserved protein in almost all kingdoms of life. As an important member of the NEET (Asn-Glu-Glu-Thr) superfamily, MiNT (Miner2) involved in regulating iron and reactive oxygen species (ROS) homeostasis. It contains two CDGSH (consensus sequence: C-X-C-X2-(S/T)-X3-P-X-C-D-G-(S/A/T)-H) domains used for binding [2Fe2S] clusters.

Risk factors in systemic lupus erythematosus-associated Sjögren's syndrome: multicenter case-control studies and systematic review.

Objective: The study identifies risk factors for SLE-associated Sjögren's syndrome (SLE-SS), based on multiple cohorts and meta-analysis.

Methods: Three cohorts (Luzhou cohort, Enshi cohort, and Chengdu cohort) were initially conducted, and clinical, laboratory characteristics from patients with SLE-only and SLE-SS patients were collected and we discussed risk factors related to SLE-SS. Subsequently, a meta-analysis discussed risk factors related to SLE-SS risk from published articles and our studies.

Predictive model for coronavirus disease 2019 severity based on blood biomarkers: a retrospective study.

Objective: To develop and validate a clinical prediction model for assessing the severity of coronavirus disease 2019 (COVID-19) using blood biomarkers, aiming to support clinical decision-making and treatment guidance.

Methods: A retrospective cohort study was conducted at Beijing Shijitan Hospital on January 5, 2023, including SARS-CoV-2 positive patients with initial chest CT-detected from outpatient and emergency departments. Data on demographics, symptoms, and blood biomarkers were collected.

A Pilot Study: Does ReMind Behavior Modification Program Alleviate Symptoms of Depression After Percutaneous Coronary Intervention?

This quasi-experimental study examined the effect of ReMind (Resourcefulness + Mindfulness) behavior modification program on symptoms of depression (SoD) and long-term prognosis of patients after percutaneous coronary intervention (PCI). Four groups of quasi-experimental training were used: 12 weeks of intervention and 12 weeks of follow-up. One hundred thirty-two participants were allocated into the resourcefulness coaching group (RG), the mindfulness training group (MG), theReMind behavior modification program group (RMG), and the control group (CG) at a 1:1:1:1 ratio.

Clinical Application of 3D-Printed Custom Hemipelvic Prostheses With Re-Entrant Chiral Structure in Reconstruction After Pelvic Tumor Resection.

Objectives: Pelvic reconstruction with conventional 3D-printed prostheses faces a critical trade-off, where achieving sufficient porosity for optimal bone ingrowth often compromises essential mechanical stability. To address this challenge, this study evaluates the clinical outcomes of 3D-printed hemipelvic prostheses incorporating re-entrant chiral structure (RCS), a novel negative Poisson's ratio design, in patients undergoing pelvic reconstruction following tumor resection.

Methods: A retrospective analysis was conducted on 15 patients (eight females and seven males; mean age: 39.

Compound Kushen Injection suppresses multiple myeloma progression via TOP1-targeted coordination of DNA damage and endoplasmic reticulum stress.

Background: Multiple myeloma (MM) is a plasma cell malignancy that remains incurable due to inevitable relapse and poor treatment tolerance. Compound Kushen Injection (CKI), a traditional Chinese medicinal formulation, has been reported to exert anti-tumor effects in various cancers, but its efficacy and mechanisms in MM remain unclear.

Purpose: This study aimed to elucidate the anti-MM mechanisms of CKI, focusing on its induction of DNA damage response (DDR) and endoplasmic reticulum (ER) stress, and to assess synergy with proteasome inhibitors (PIs).

DHAG-DTA: Dynamic Hierarchical Affinity Graph Model for Drug-Target Binding Affinity Prediction.

Computational methods for predicting drug-target binding affinity (DTA) are critical for large-scale screening of prospective therapeutic compounds during drug discovery. Deep neural networks (DNNs) have recently shown significant promise for DTA prediction. By leveraging available data for training, DNNs can expand the use of DTA prediction to situations where only sequence information is available for potential drug molecules and their targets, and there is no prior knowledge regarding the molecular geometric conformations.

Food Bioactive for Gut-Metabolic Axis Regulation and Microbiota Modulation.

The escalating global burden of metabolic diseases, immune dysfunction, and age-related degeneration underscores the imperative for innovative nutritional interventions [...

Analyses of NOTCH3 variants in Chinese patients with clinically diagnosed Alzheimer's disease and frontotemporal dementia.

Background: A pathogenic variant in the NOTCH3 gene has been identified as the cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). Studies focusing on variants in NOTCH3 in Alzheimer's disease (AD) and frontotemporal dementia (FTD) cohorts have been limited. We aim to screen clinically diagnosed AD and FTD patients with unknown etiology for pathogenic variants in NOTCH3 in the Chinese population.

TQFL13, a novel synthetic derivative of thymoquinone (TQ), is more capable of inhibiting breast cancer progression than TQ.

Thymoquinone (TQ) is a bioactive component derived from Nigella Sativa seeds, and its anticancer properties have been well-documented. Our preliminary studies have also shown that the effective concentration of TQ against breast cancer (BC) is slightly high, indicating a need for chemical modification and optimization of the TQ compound. To address this, we designed and synthesized numerous TQ derivatives and conducted in-depth studies on the 13th derivative, TQFL13 from our laboratory.

CDFA: Calibrated deep feature aggregation for screening synergistic drug combinations.

Introduction: Drug combination therapy represents a promising strategy for addressing complex diseases, offering the potential for improved efficacy while mitigating safety concerns. However, conventional wet-lab experimentation for identifying optimal drug combinations is resource-intensive due to the vast combinatorial search space. To address this challenge, computational methods leveraging machine learning and deep learning have emerged to effectively navigate this space.

[Map-based cloning and abiotic stress response analysis of in rice].

Rice ( L.) is an important food crop. The appearance of lesion mimics in rice leads to phytohormone disorders, which affects rice adaptation to environmental stresses and ultimately reduces the yield and quality.

Statistical Insights into MOF Design: A Pillar-Layered MOF with High-Density N/O Sites for One-Step C2 Separation.

Although the one-step separation of ethylene from C2 hydrocarbons remains a significant challenge, one-step C2 separation provides a revolutionary breakthrough in cost savings and efficient energy utilization. Recent advances in metal-organic frameworks (MOFs) for achieving one-step C2 separation have been widely recognized. In this work, for the first time, the potential relationship between the structure and properties of MOF materials previously used for one-step C2 separation was revealed from a mathematical statistical perspective.

Jab1 promotes immune evasion and progression in acute myeloid leukemia models under oxidative stress.

Acute myeloid leukemia (AML) is the most common hematological malignancy. Leukemia stem cells exhibit high levels of oxidative stress, with reactive oxygen species (ROS) being the primary products of this stress, inducing the expression of Jab1. Previous studies have demonstrated that Jab1, as a transcriptional coactivator of c-JUN, promotes the malignant progression of AML under oxidative stress.

Design of Mott Multiferroic HfO by Inducing Unpaired d States.

Single-phase multiferroics are rare due to the incompatibility of the empty d orbitals for ferroelectricity and partially filled d orbitals for magnetism. This study proposes a route to achieve Mott multiferroicity in HfO via Nb and Ta doping, which induces an extra d electron while preserving the oxygen displacement in the polar phase. Density functional theory (DFT) calculations confirm the stability of doped ferroelectric (FE) HfO, with FE transition temperatures exceeding room temperature and reduced switching barriers.

Effect analysis of flexible bronchoscopy in thoracoscopic surgery for congenital pulmonary airway malformation.

The objective of this study is to investigate the clinical efficacy of flexible bronchoscopy (FB) in thoracoscopic surgery for congenital pulmonary airway malformation (CPAM) in pediatric patients. We randomly divided the CPAM patients who underwent thoracoscopic surgery at our hospital from May 2022 to January 2024 into 2 groups based on whether they received FB treatment before and after thoracoscopic surgery. Group A included 24 patients who received FB treatment, while Group B consisted of 21 patients who underwent conventional thoracoscopic surgery.

Azelaic acid attenuates CCL2/CCR2 axis-mediated skin trafficking of acute myeloid leukemia cells through NF-κB/MAPK signaling modulation in keratinocytes.

Background: The involvement of CCL2/CCR2 in leukemic cell infiltration into the skin, specifically in leukemia cutis (LC), remains largely unexplored. Oxidative stress has been demonstrated to induce the upregulation of CCL2 expression, while the antioxidant properties of azelaic acid (AZA) have been proven to exert anti-acute myeloid leukemia (AML) effects.

Aims: To investigate the role of CCL2/CCR2 in the pathogenesis of leukemia cutis, and to evaluate whether azelaic acid (AZA) can inhibit the infiltration of leukemic cells into the skin and its possible mechanism.

Three-dimensional printing in modern orthopedic trauma surgery: a comprehensive analysis of technical evolution and clinical translation.

Three-dimensional (3D) printing has emerged as a transformative technology in orthopedic trauma surgery, offering unprecedented possibilities for personalized surgical solutions. Despite its increasing adoption, there remains a lack of comprehensive reviews systematically evaluating its technical considerations and evidence-based outcomes across different anatomical regions. Through systematic review of literature from major databases and analysis of clinical evidence, this comprehensive review examines the current state of advanced 3D printing technologies in orthopedic trauma.

Efficacy of paliperidone palmitate once-monthly (PP1M) in improving social functioning and reducing caregiver burden in patients with schizophrenia: a six-month follow-up study.

Background: Schizophrenia is a complex chronic mental disorder that severely impairs patients' social functioning and quality of life, while imposing substantial burdens on families and society. Long-acting injectable antipsychotics (LAIs) have demonstrated potential advantages in improving both symptoms and social functioning. This study investigated the effects of transitioning from oral antipsychotics (OAPS) to Paliperidone Palmitate Once-Monthly (PP1M) on symptom control, social functioning, and caregiver burden in schizophrenia patients.