Utilizing network pharmacology and other tools to examine active components and mechanism of action of Magnolia officinalis rheum rhabarbarum decoction in treating Streptococcus pyogenes skin infections.

Infections caused by Streptococcus pyogenes and the growing threat of antibiotic resistance pose significant global health challenges. This study investigates the antibacterial properties of Magnolia officinalis Rheum rhabarbarum Decoction against Streptococcus pyogenes skin infections. By combining UHPLC-MS/MS, network pharmacology, and molecular docking techniques, we identified eight bioactive compounds in the formulation and explored their potential interactions with Streptococcus pyogenes-related targets.

Efficacy analysis of fiberoptic bronchoscopy combined with thoracoscopy in the treatment of mediastinal bronchogenic cysts in children.

This study explores the clinical efficacy of perioperative application of fiberoptic bronchoscopy (FB) combined with thoracoscopic minimally invasive surgery for mediastinal bronchogenic cysts in children. A retrospective analysis was conducted on 48 pediatric patients with mediastinal tumors who were admitted to our hospital from January 2015 to December 2023. Postoperative pathological results confirmed the diagnosis of bronchogenic cysts.

A single-phase epitaxially grown ferroelectric perovskite nitride.

The integration of ferroelectrics with semiconductors is crucial for developing functional devices, such as field-effect transistors, tunnel junctions, and nonvolatile memories. However, the synthesis of high-quality single-crystalline ferroelectric nitride perovskites has been limited, hindering a comprehensive understanding of their switching dynamics. Here we report the synthesis and characterizations of epitaxial single-phase ferroelectric cerium tantalum nitride (CeTaN) on both oxides and semiconductors.

Effect analysis of flexible bronchoscopy in thoracoscopic surgery for congenital pulmonary airway malformation.

The objective of this study is to investigate the clinical efficacy of flexible bronchoscopy (FB) in thoracoscopic surgery for congenital pulmonary airway malformation (CPAM) in pediatric patients. We randomly divided the CPAM patients who underwent thoracoscopic surgery at our hospital from May 2022 to January 2024 into 2 groups based on whether they received FB treatment before and after thoracoscopic surgery. Group A included 24 patients who received FB treatment, while Group B consisted of 21 patients who underwent conventional thoracoscopic surgery.

Transcription factor GbTCP20 confers plant wilt resistance by regulating ACC biosynthesis pathway and lignin deposition.

As a family of plant-specific transcription factors, the TEOSINTE BRANCHED 1, CYCLOIDEA, and PROLIFERATING CELL FACTOR (TCP) family plays a pivotal role in biotic stress responses. Verticillium dahliae, a devastating soil-borne fungal pathogen, causes severe vascular wilt in numerous crops. Several studies have reported that TCPs modulate defense signaling against various pathogens, while their molecular mechanisms in regulating the resistance to V.

Hair follicle epithelial stem cells contribute to interfollicular epidermis during homeostasis.

Mammalian skin is a vital barrier with the epidermis serving as its protective outer layer, continually undergoing renewal. Given that loss of the epidermis or its barrier function is lethal for mammals, multiple stem cell populations likely exist for the interfollicular epidermis (IFE), enhancing evolutionary survival. Here, we demonstrate that transcription factor KROX20 marks a heterogeneous stem cell population in the upper and middle mouse hair follicle (HF), partially overlapping with known HF stem cell markers in those regions.

Evaluation of Large-Scale Plasma Proteomics for Prediction of Heart Failure in Individuals with A Full Range of Glucose Metabolism Profiles.

Aims: Individuals with abnormal glucose metabolism are at a significantly higher risk of developing heart failure (HF). However, strategies for early identification of HF in this high-risk population remain inadequate. This study aimed to identify plasma protein biomarkers associated with HF development and construct predictive models to identify at-risk individuals.

Case Report: Fiberoptic bronchoscopy and thoracoscopic surgery as a treatment for pulmonary mucormycosis in pediatric acute lymphoblastic leukemia.

Background: Mucormycosis, a severe disease caused by fungal infections of the Mucorales order, is a frequent cause of mortality in patients with hematological malignancies. Pulmonary mucormycosis represents a common form of this condition in pediatric patients with hematological malignancies and is associated with a high mortality rate. Liposomal amphotericin B (L-AmB) remains the primary therapeutic agent for pulmonary mucormycosis, however, its efficacy is often limited.

Experimental study on the low-temperature performance and evaluation methods of crack sealants for asphalt pavements in seasonal frozen regions.

Road repair materials employed in the seasonal frozen regions of Northeastern China always often demonstrate poor long-term performance under extreme climate conditions. This is primarily attributed to the detrimental effects of short-term aging and frequent freeze-thaw cycles on the adhesive properties of sealants. Existing standards fail to adequately account for these complex environmental factors, leading to unsatisfactory repair outcomes.

A Self-healing, Antibacterial, Antioxidant, Injectable Hydrogel Containing Tannic Acid for Skin Wound Repair.

Skin defects resulting from various causes are common issues in clinical practice. The predominant approach to skin wound repair involves the application of wound dressings to facilitate healing. However, the current treatment methods face significant limitations, including insufficient functional restoration and inadequate blood supply.

Chemogenetic Regulation in Reprogrammed Stem Cell-derived Precursor Cells in Treating Neurodegenerative Diseases.

The integration of designer receptors exclusively activated by designer drugs (DREADD) with stem cell-based therapies presents an advanced strategy for precise neuronal modulation. Here, we utilized CRISPR-engineered human reprogrammed stem cells expressing excitatory (hM3Dq) or inhibitory (hM4Di) DREADD receptors to evaluate the functional integration and modulation of transplanted dopaminergic precursors in a murine model of Parkinson's disease (PD). Key steps included generating non-fusion DREADD-expressing stem cell lines, differentiating them into midbrain dopaminergic precursors, and transplanting these cells into the striatum of 6-hydroxydopamine (6-OHDA)-lesioned mice.

The comorbidity of anxiety and depression symptoms in obsessive-compulsive disorder: a network analysis.

Objective: People with obsessive-compulsive disorder (OCD) frequently suffer from comorbid anxiety and depression. From the perspective of the network model, this comorbidity is thought to be an interacting system of three symptoms. In the study, we conducted a network analysis of anxiety and depression comorbidity in OCD, aiming to identify the central and bridge symptoms and make informed suggestions for clinical interventions and psychotherapy.

Immune-related biomarkers in the neuromyelitis optica spectrum disorder; pathogenesis and therapeutic approaches.

Neuromyelitis optica spectrum disorder (NMOSD) is an autoimmune inflammatory disease of the central nervous system that mostly affects the optic nerves and spinal cord. About eighty percent of patients have antibodies that are directed against the water channel aquaporin-4 (AQP4)-IgG, which is expressed on astrocytes. This protein was shown to be both a biomarker and a pathogenic cause of NMOSD.

Constitutive Hepatic mTORC1 Activation Aggravates Alcohol-Induced Liver Injury via Endoplasmic Reticulum Stress-Mediated Ferroptosis.

Alcohol-related liver disease (ALD), a consequence of excessive alcohol use, manifests across a broad spectrum of liver damage, ranging from steatosis to cirrhosis. DEPDC5 (DEP domain-containing protein 5) is a component of the GATOR1 (gap activity towards rags 1) complex, which functions as a repressor of the amino acid-sensing branch of the mammalian target of rapamycin complex 1 (mTORC1) pathway. In this study, hepatocyte-specific Depdc5 knockout mice (Depdc5) were generated.

Robot-Assisted Stereotactic Microinjection Method for Precision Cell Transplantation in Rat and Canine Models.

Cell transplantation is a promising approach for addressing neurodegenerative conditions. In this study, we developed a robot-assisted stereotactic microinjection system for transplanting cells. We evaluated the factors that affect cellular graft viability and other properties, including the gauge of the syringe needle and the injection rate.

Enhanced Phonon-Phonon Interactions and Weakened Electron-Phonon Coupling in Charge Density Wave Topological Semimetal EuAl with a Possible Intermediate Electronic State.

The origin of the charge density wave (CDW) is a long-term open issue. Furthermore, the evolution of phonon-phonon interactions (PPIs) across CDW transitions has rarely been investigated. Whether electron-phonon coupling (EPC) would be weakened or enhanced after CDW transitions is still under debate.

Effects of NUSS surgery on degree of flat thoracic cage, cardiopulmonary function, and bone metabolism in pectus excavatum patients of different ages.

This study was to explore the degree of flat thoracic cage (FTC), cardiopulmonary function, and bone metabolism of pectus excavatum (PE) children of different ages after NUSS surgery. A retrospective analysis was conducted on 90 children with PE who underwent minimally invasive pectus excavatum correction surgery (NUSS), divided into three age groups: group I (1-3 years), group II (3-6 years), and group III (6-12 years), with 30 patients in each group. The study compared differences in thoracic flattening index, surgical-related metrics, pulmonary function, peripheral blood levels of alkaline phosphatase (ALP), calcium, magnesium, zinc, and phosphorus, and complication rates among the groups.

A multiomic atlas identifies a treatment-resistant, bone marrow progenitor-like cell population in T cell acute lymphoblastic leukemia.

Refractoriness to initial chemotherapy and relapse after remission are the main obstacles to curing T cell acute lymphoblastic leukemia (T-ALL). While tumor heterogeneity has been implicated in treatment failure, the cellular and genetic factors contributing to resistance and relapse remain unknown. Here we linked tumor subpopulations with clinical outcome, created an atlas of healthy pediatric hematopoiesis and applied single-cell multiomic analysis to a diverse cohort of 40 T-ALL cases.

Albumin binding domain fusion improved the therapeutic efficacy of Inhibitor of Differentiation-2 protein in colitis mice.

Aims: The human Inhibitor of Differentiation-2 (hID2) protein is a promising candidate for the treatment of colitis. However, its relatively low molecular weight limits its clinical application. To extend the therapeutic half-life, an albumin-binding domain (ABD), known for its high affinity for human serum albumin (HSA), was fused to hID2, resulting in a recombinant ABD-hID2.

Solid-State Reversible Phase Transition and Photoluminescence Properties of Organic-Inorganic Hybrid Halide: (BTPA)MnBr.

Multifunctional materials have long been a popular research area, with organic-inorganic hybrids frequently utilized due to their diverse properties and versatile assembly techniques. In this context, a novel Mn-based organic-inorganic hybrid compound (BTPA)MnBr() was prepared, with strong green photoluminescence, phase transition under thermal stimulation, and two reversible dielectric-state switches. This compound exhibits strong green photoluminescence under ultraviolet excitation, boasting a quantum yield of 44.

The isoflavone puerarin promotes generation of human iPSC-derived pre-oligodendrocytes and enhances endogenous remyelination in rodent models.

Puerarin, a natural isoflavone, is commonly used as a Chinese herbal medicine for the treatment of various cardiovascular and neurological disorders. It has been found to be neuroprotective via TrK-PI3K/Akt pathway, which is associated with anti-inflammatory and antioxidant effects. Myelin damage in diseases such as multiple sclerosis (MS) and ischemia induces activation of endogenous oligodendrocyte progenitor cells (OPC) and subsequent remyelination by newly formed oligodendrocytes.

Transcription factor KROX20 marks epithelial stem cell ancestors for hair follicle formation.

Epidermal stem cells control homeostasis and regeneration of skin and hair. In the hair follicle (HF) bulge of mammals, populations of slow-cycling stem cells regenerate the HF during cyclical rounds of anagen (growth), catagen (regression), and telogen (quiescence). Multipotent epidermal cells are also present in the HF above the bulge area, contributing to the formation and maintenance of sebaceous gland and upper and middle portions of the HF.