Unexpected renal influence on isavuconazole trough concentrations: two case reports and literature review.

Isavuconazole (ISA) is a first-line treatment for invasive aspergillosis, with routine therapeutic drug monitoring (TDM) typically deemed unnecessary. Although the drug label and prior studies suggest renal function does not affect ISA pharmacokinetics, our findings in two high-risk patients challenge this perspective, showing an inverse correlation between ISA trough concentrations and estimated glomerular filtration rate (eGFR). Trough concentrations exceeding the reported toxicity threshold were associated with adverse drug reactions (ADRs).

New cardiac glycosides from Adonis amurensis with potent antitumor activity.

Twenty-four cardiac glycosides - including six previously undescribed compounds (1-6) and eighteen known analogues (7-24) - were isolated from the whole herb of Adonis amurensis. Their structures were elucidated through comprehensive spectroscopic analysis (1D/2D NMR, HRMS) and acid hydrolysis. All compounds exhibited potent in vitro cytotoxicity against diverse cancer cell lines, with IC₅₀ values ranging from 0.

Diversity, evolution and transcription of endogenous retroviruses in Chiroptera genomes.

Bats (Chiroptera) are a taxonomic group of immense biological and ecological importance. They are primary reservoirs and carriers of various zoonotic viruses. Endogenous retroviruses (ERVs) originate from ancient retroviruses invading the host, and ERV-derived sequences can function as regulatory elements which influence gene expression and contribute to both physiological and pathological processes.

Antibody-free quantification of serum infliximab using LC-MS/MS.

Infliximab (IFX) is the first biologic to be approved to treat inflammatory bowel disease (IBD). Serum IFX concentrations can be correlated with the clinical prognosis of IBD. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is an alternative to clinical immunoassays owing to its higher selectivity and sensitivity.

Interface-controlled uniaxial in-plane ferroelectricity in HfZrO(100) epitaxial thin films.

Hafnium oxide-based ferroelectric thin films are widely recognized as a CMOS-compatible and highly scalable material platform for next-generation non-volatile memory and logic devices. While out-of-plane ferroelectricity in hafnium oxide films has been intensively investigated and utilized in devices, purely in-plane ferroelectricity of hafnium oxides remains unexplored. In this work, we demonstrate a reversible structural modulation of the orthorhombic phase HfZrO films between (111)-oriented [HZO(111)] multi-domain and (100)-oriented [HZO(100)] single-domain configurations by altering perovskite oxide buffer layers.

Enhancing clinical reasoning skills through tailored CPC in pathology laboratory instruction.

Background: In medical education, fostering students' clinical reasoning skills is crucial for assessing their clinical proficiency. However, developing a Clinical Pathological Conference (CPC) model tailored to the unique needs of early-stage learners remains an intriguing avenue for exploration.

Methods: This study aimed to enhance the clinical reasoning and critical thinking abilities of early-stage medical students in pathology laboratory instruction through the introduction of a customized CPC teaching method.

Sex Differences in Functional Gradients and Dynamic Functional Connectivity in Preschool-Aged Children With ASD.

Background: The prevalence of autism spectrum disorder (ASD) is significantly higher in males than in females; although the underlying etiology remains unclear. This study aimed to investigate the multi-scale reorganization of brain networks in preschool-aged boys with ASD and their impact on clinical symptoms.

Methods: A total of 54 children with ASD (40 boys and 14 girls) and 44 typically developing (TD) children (28 boys and 16 girls), aged between 2 and 6 years, were recruited for this study.

A single-phase epitaxially grown ferroelectric perovskite nitride.

The integration of ferroelectrics with semiconductors is crucial for developing functional devices, such as field-effect transistors, tunnel junctions, and nonvolatile memories. However, the synthesis of high-quality single-crystalline ferroelectric nitride perovskites has been limited, hindering a comprehensive understanding of their switching dynamics. Here we report the synthesis and characterizations of epitaxial single-phase ferroelectric cerium tantalum nitride (CeTaN) on both oxides and semiconductors.

Research on the Hormonomics of Three Species and Their Flavonoid Diversification and Specificity.

Hormonomics represents an innovative approach to plant physiology and biochemistry. We utilized hormonomics to analyze the hormone profiles of three lily bulbs. The hormones specifically enriched in BiFeng7 lily show a strong response to secondary metabolism pathways, while the Diwanghuang lily profile was predominantly focused on growth.

Lymph node metastasis in patients with hepatocellular carcinoma using machine learning: a population-based study.

Aim: This study aims to develo\p a population-adapted machine learning-based prediction model for hepatocellular carcinoma (HCC) lymph node metastasis (LNM) to identify high-risk patients requiring intensive surveillance.

Methods: Data from 23511 HCC patients in the SEER database and 57 patients from our hospital were analyzed. Seven LNM risk indicators were selected.

Is Everything Everywhere? Dispersal Limitation Impacts Methanotroph Community Functioning.

The significance of dispersal limitation in microbial ecology and biogeography remains debated. We aimed to clarify the role of dispersal limitation in the adaptation of methanotroph communities to salt-stress, essentially testing the 'everything is everywhere' hypothesis in functional terms. Riparian sediments along the Yangtze River and lakeshore sediments at varying geographical distances inland from the river were collected.

Hippo/YAP signaling's multifaceted crosstalk in cancer.

The Hippo/yes-associated protein (YAP) signaling is an evolutionarily conserved regulator in organ size control, which plays pivotal roles in cell proliferation, differentiation, apoptosis, and tissue regeneration. In cancer, dysregulation of Hippo/YAP signaling is typically recognized as one of the crucial drivers in tumorigenesis. However, beyond its canonical transcriptional targets, Hippo/YAP signaling engages in extensive crosstalk with multiple pathways to form an intricate regulatory network, thereby giving rise to its content-dependent influence on tumor initiation, progression and metastasis.

Structural insights into transcriptional regulation by the helicase RECQL5.

Transcription poses a major challenge for genome stability. The RECQL5 helicase helps safeguard genome integrity and is the only member of the human RecQ helicase family that directly binds to RNA polymerase II (Pol II) and affects its progression. While RECQL5 mitigates transcription stress in cells, the molecular mechanism underlying this phenomenon is unclear.

Efficacy of the 'three ponds and two dams' ecological treatment system in purifying shrimp pond wastewater and mitigating antibiotic resistance.

Shrimp pond aquaculture, like fish pond culture, faces significant challenges such as water pollution and antibiotic resistance, driven by chemical overuse and untreated effluent discharge. This study evaluates the efficacy of an ecological treatment system in treating shrimp pond wastewater, focusing on water quality improvement, microbial community dynamics, phytoplankton composition, and antibiotic resistance gene (ARG) mitigation. Using adjacent river indicators as a reference, comparative analyses were conducted on phytoplankton density and biomass, key water quality parameters, and microbial parameters in shrimp culture and ecological ponds across key growth stages and the pond cleaning period.

Superhydrophobic Conductive Materials: System Design, Processing Adjustment, and Promising Applications.

The working efficiency, reliability, and stability of electronic materials and devices in real environments often face challenges from humid conditions, aging, mildew, chemical damage (especially corrosion), and physical damage (like freezing and abrasion). To address these issues, endowing conductive materials with bionic superhydrophobicity offers promising solutions by providing them with wet-resistant, antifreezing, anticorrosion, antifouling, and other functions. Through different methods including immersion, coating, spraying, solvothermal reaction, and layer-by-layer (LBL) self-assembly, etching and screen printing, etc.

Prediagnostic plasma metabolite concentrations and liver cancer risk: A nested case-control study with long follow-up in the Shanghai Women's Health Study.

Present knowledge on metabolic perturbation prior to liver cancer is limited, with most studies unable to quantify the absolute concentrations of metabolites and lacking a focus on female-specific findings. We conducted a 1:1 matched nested case-control study (187 pairs of cases and controls) within the Shanghai Women's Health Study. A targeted metabolomics method was used to quantify 186 metabolites in plasma samples collected at recruitment.

Family socio-economic status and parental education anxiety: the mediating role of perception of the "double reduction" policy and the moderating role of parental education expectations.

Background: The "Double Reduction" policy promulgated by the Chinese government in 2021 aims to alleviate parental education anxiety as one of the policy goals, blocking the path of parents' high participation in their children's education through intensive out-of-school training and a large number of homework exercises in the past, to comprehensively promote educational equity. However, with the continuous promotion of the policy since its initial introduction, it now faces new controversies. Parents from varying family socio-economic backgrounds exhibit divergent responses to the "Double Reduction" policy, necessitating an examination of parental education anxiety through the lens of family socio-economic status at this juncture.

β-propeller protein-associated neurodegeneration protein WDR45 regulates stress granule disassembly via phase separation with Caprin-1.

β-propeller protein-associated neurodegeneration (BPAN) is a rare X-linked neurodegenerative disorder caused by mutations in the WDR45 gene, yet its molecular mechanisms remain poorly understood. Here, we identify a role for WDR45 in stress granule (SG) disassembly, mediated through its phase separation with Caprin-1. We demonstrate that WDR45 forms gel-like condensates via its WD5 domain, which competitively displaces G3BP1 from Caprin-1 to promote SG disassembly.

Association of Genetic Variants With Rivaroxaban Pharmacokinetics and Pharmacodynamics and Hemorrhage Risk Factors in Patients With Venous Thromboembolism.

Background: Rivaroxaban is a first-line agent for venous thromboembolism prophylaxis and treatment. However, its pharmacokinetics, pharmacodynamics, and bleeding risk exhibit significant interindividual variability. Known nongenetic factors alone cannot fully explain this variability, and the impact of genetic polymorphisms remains debated.

A single-center case-control study of the association between dorsal striatal damage and nicotine addiction.

Background: Smoking is closely linked to pulmonary diseases, cardiovascular diseases, cancer, and other health issues, posing a significant threat to human health. The essence of smoking addiction lies in nicotine dependence, though the underlying mechanisms remain unclear. Previous studies have suggested that nicotine addiction is associated with regions such as the insula, globus pallidus, amygdala, and ventral striatum.

The Complete Mitochondrial Genome of Red Costate Tiger Moth ( [Cramer, 1777]), and Phylogenetic Analyses of the Subfamily Arctiinae.

Background/objectives: , class Insecta, order Lepidoptera, superfamily Noctuoidea, family Erebidae, and subfamily Arctiinae, is a polytrophic agricultural pest. However, there are still many sequences missing for Arctiinae from mitochondrial whole-genome sequences.

Methods: In this study, we determined and analyzed the complete mitochondrial genome sequence of .

Complete Mitochondrial Genome of (Odonata: Anisoptera: Chlorogomphidae) and Phylogenetic Analyses.

This study aimed to elucidate the mitochondrial genome organization of and the phylogenetic relationships of Chlorogomphidae. We used the Illumina MiSeq sequencing platform to sequence the mitochondrial genome of , which was subsequently assembled, annotated, and analyzed. Bayesian inference, maximum likelihood, and maximum parsimony methods were employed to construct the mitochondrial phylogenetic tree of 25 species of Chlorogomphidae based on and genes.

The emerging roles of N6-methyladenosine (m6A) deregulation in polycystic ovary syndrome.

Polycystic ovary syndrome (PCOS) is an endocrine metabolic syndrome characterized by ovulation disorders, hyperandrogenemia, and polycystic ovaries, which seriously affect the psychological and physical health of childbearing women. N6-methyladenosine (m6A), as the most common mRNA epigenetic modification in eukaryotes, is vital for developing the female reproductive system and reproductive diseases. In recent years, an increasing number of studies have revealed the mechanisms by which m6A modifications and their related proteins are promoting the development of PCOS, including writers, erasers and readers.

Qualitative and quantitative determination of SIPI-2011 and its two major metabolites in human plasma.

SIPI-2011, a structural modification of isoquinoline alkaloid, is under investigation for treating arrhythmias. To characterize the safety and tolerability, the pharmacokinetics and metabolism of SIPI-2011 were investigated in humans. After an oral administration of 600 mg SIPI-2011, a total of 32 metabolites were detected in human plasma by UPLC-UV/Q-TOF mass spectrometry utilizing mass defect filter method.

Association of Vancomycin Plus Piperacillin-Tazobactam With Acute Kidney Injury: Differentiating Pseudo-Injury From True Nephrotoxicity.

The combination of vancomycin and piperacillin-tazobactam (VPT) is widely used for severe infections, but its potential synergistic nephrotoxicity remains debated. This study used the FDA Adverse Event Reporting System (FAERS) database via AERSMine to assess the risk of AKI with VPT and vancomycin plus carbapenems (VC), compared to other vancomycin-based regimens to evaluate the hypothesis of pseudo-nephrotoxicity versus true nephrotoxicity. Disproportionality analysis was performed using the reporting odds ratio (ROR) and Bayesian confidence propagation neural network (BCPNN).