3D map-guided modeling of functional endometrial tissue using multi-compartment assembloids.

The human endometrium is a dynamic tissue that lines the uterus and undergoes constant remodeling, making it especially susceptible to gynecological diseases like endometriosis and endometrial cancer. The molecular mechanisms of these conditions are not well understood, partly due to the lack of in vitro models that mimic endometrial physiology, which limits options for targeted intervention and treatment of these diseases. Mouse models are also inadequate, as common laboratory strains do not naturally undergo a menstrual cycle comparable to that of humans.

Spontaneous reshaping of microalgae carbon metabolism under organophosphate esters stress: Insights into adaptive strategies for wastewater treatment.

Microalgae have shown adaptive responses to emerging pollutants, yet the underlying carbon metabolic mechanisms remain poorly understood. This study integrated physiological, transcriptomic and bioinformatic analysis to investigate carbon flux remodeling in Chlorella sorokiniana exposed to 1-10 mg/L organophosphate esters (OPEs) during the adaptive phase of microalgal growth in synthetic wastewater. OPEs inhibited sodium acetate (NaAc) uptake by 10.

Prognostic Value of Myocardial T1 Mapping and Extracellular Volume Fraction in Heart Failure: A Meta-Analysis.

Background: Heart failure (HF) is a global health burden. Myocardial fibrosis is a crucial promotor in HF progression which can be characterized noninvasively by cardiac magnetic resonance T1 mapping.

Objectives: This study aims to systemically evaluate the prognostic value of myocardial T1 mapping and extracellular volume fraction (ECV) covering the entire spectrum of HF through a meta-analysis.

Population structure and genome-wide association study of body conformation traits of two native goat breeds in China.

Objective: Body conformation traits have a direct impact on carcass performance in the meat goat industry. This study explored the population genetics of two Chinese goat breeds and identified the genomic variates contributing to their body conformation traits.

Methods: The Guizhou black goat (GBG, n=104) and Hezhang black goat (HBG, n=100) were genotyped by whole-genome sequencing and phenotyped by measuring their body height (BH), body length (BL), chest depth (CD), chest width (CW), chest girth (CG), rump width (RW), rump height (RH), and cannon circumference (CC).

Superior High-Temperature Energy Storage Performance in All-Organic Composite Dielectrics Achieved by Synergistic Regulation of Free Volume and Charge Distribution.

Developing polymer dielectrics with stable high-temperature energy storage performance remains a pivotal challenge for next-generation electrical systems. However, the exponentially increasing conduction loss at elevated temperatures results in a decline in both energy storage density and efficiency. Herein, a cross-scale synergistic regulation strategy that integrates mesoscale free volume and molecular-scale charge trap, effectively addressing the issue of free volume collapse and space charge accumulation under thermal-electric coupling stress is proposed.

Risk stratification and outcomes in diabetes mellitus patients with preserved ejection fraction: a cardiac MRI study.

Background: Patients with diabetes mellitus (DM) have a significantly increased risk of developing heart failure (HF), which exacerbates adverse cardiovascular outcomes. Limited data are available on the prognostic value of cardiac MRI in DM. We aimed to evaluate the association between MRI-derived strain analysis and adverse outcomes in DM patients at different heart failure (HF) stages.

The mechanisms of antibiotic resistance and drug resistance transmission of Klebsiella pneumoniae.

Klebsiella pneumoniae is a ubiquitous Gram-negative pathogen in clinical settings, responsible for pulmonary infections, urinary tract infections, septicemia, and other severe diseases.Over recent years, widespread use of aminoglycosides, quinolones, and β-lactams has driven a marked increase in antimicrobial resistance, compromising treatment efficacy. Here, we review the molecular bases of K.

New cardiac glycosides from Adonis amurensis with potent antitumor activity.

Twenty-four cardiac glycosides - including six previously undescribed compounds (1-6) and eighteen known analogues (7-24) - were isolated from the whole herb of Adonis amurensis. Their structures were elucidated through comprehensive spectroscopic analysis (1D/2D NMR, HRMS) and acid hydrolysis. All compounds exhibited potent in vitro cytotoxicity against diverse cancer cell lines, with IC₅₀ values ranging from 0.

T Cell Involvement in Neuroinflammation After Traumatic Brain Injury: Implications for Therapeutic Intervention.

Background: Traumatic brain injury (TBI) is a leading cause of death and disability across all age groups worldwide. After primary mechanical head injury, a cascade of molecular changes and immunological responses occur that are necessary for supporting tissue repair but also exacerbate the secondary loss of tissue caused by excessive neuroinflammation. To date, there are no targeted treatments that ameliorate the pathological neuroinflammation that is responsible for propagating secondary injury after TBI.

Anti-windup compensator based on LADRC and its application in the attitude control of near-space aircraft.

A novel Anti-windup Compensator (AWC) is proposed for the Linear Extended State Observer (LESO) in the Linear Active Disturbance Rejection Controller (LADRC). Unlike classical AWC structures, this compensator augments LADRC by dynamically adjusting state variables and extended state compensation within the LESO, resulting in the method being termed AWC-LESO. The adjustment is based on the degree of saturation, effectively mitigating the impact of input saturation on the system.

The Volatile Oil of Dried Ginger Promotes Intestinal Epithelial Renewal through T-UCR 481.

Dried ginger is widely used in condiments and in food materials. The volatile oil in ginger can regulate intestinal flora and affect the intestinal barrier function. First, we extracted the volatile oil of dried ginger (VOG) and analyzed it by gas chromatography-mass spectrometry (GC-MS).

Phenotypic stratification and prognostic value of cardiac magnetic resonance in non-dilated left ventricular cardiomyopathy.

Background: Non-dilated left ventricular cardiomyopathy (NDLVC), characterised by non-ischaemic scar/fatty replacement or isolated systolic dysfunction without dilatation, lacks validated risk stratification tools. We aimed to define cardiac magnetic resonance (CMR)-based phenotypes and evaluate their association with clinical outcomes.

Methods: In 515 patients with NDLVC (mean age 45 (16) years), three phenotypes were classified by CMR: late gadolinium enhancement (LGE+)/H- (LGE with preserved left ventricular ejection fraction (LVEF), n=130), LGE-/H+ (hypokinesia without LGE, n=226) and LGE+/H+ (LGE with reduced LVEF, n=159).

Structural characterization of two glucans from Saposhnikovia divaricata and evaluation of their anti-neuroinflammatory activity.

Saposhnikovia divaricata (Turcz.) Schischk. has been widely used as an important Chinese herbal medicine in the field of medicine, agriculture and other fields where it has been demonstrated to have significant economic value.

Genome-wide investigation of transcription factor occupancy and dynamics using cFOOT-seq.

Gene regulation relies on the precise binding of transcription factors (TFs) at regulatory elements, but simultaneously detecting hundreds of TFs on chromatin is challenging. We developed cFOOT-seq, a cytosine deaminase-based TF footprinting assay, for high-resolution, quantitative genome-wide assessment of TF binding in both open and closed chromatin regions, even with small cell numbers. By utilizing the dsDNA deaminase SsdAtox, cFOOT-seq converts accessible cytosines to uracil while preserving genomic integrity, making it compatible with techniques like ATAC-seq for sensitive and cost-effective detection of TF occupancy at single-molecule and single-cell level.

Domain adaptive deep possibilistic clustering for EEG-based emotion recognition.

Emotion recognition based on electroencephalogram (EEG) faces substantial challenges. The variability of neural signals among different subjects and the scarcity of labeled data pose obstacles to the generalization ability of traditional domain adaptation (DA) methods. Existing approaches, especially those relying on the maximum mean discrepancy (MMD) technique, are often highly sensitive to domain mean shifts induced by noise.

From antiferromagnetism to field-induced ferromagnetism: A Cu-governed metamagnetic landscape in RFeCuGeO (R = Tm-Lu).

Metamagnetism represents a distinctive subclass within the antiferromagnetic (AFM) regime, exhibiting significant potential for diverse technological applications, particularly in magnetocaloric effects. In this paper, we systematically investigate the magnetic-field-induced phase transition in RFeCuGeO (R = Tm-Lu) through comprehensive temperature-dependent and field-dependent magnetization measurements and neutron powder diffraction (NPD) analysis. Our experimental results demonstrate an AFM transition at ∼18, 13 and 13.

A comprehensive review of the pathogenic mechanisms of : synergistic effects of virulence factors, quorum sensing, and biofilm formation.

() is a ubiquitous opportunistic pathogen and a major cause of nosocomial infections worldwide. It can provoke a spectrum of clinical manifestations-ranging from postoperative wound infections, pressure ulcers, abscesses, and otitis media to life-threatening bacteremia and sepsis, especially in burn patients. Over the past decade, extensive research has elucidated its complex virulence repertoire, including exotoxins, proteases, and siderophores; the hierarchical Quorum Sensing (QS) networks; and its robust capacity for biofilm formation.

Epidemiological and biological characteristics of IncR plasmids as multihost antibiotic resistance carriers.

IncR plasmids are important resistance vectors broadly disseminated among various Gram-negative bacteria. Their capacity for inter-host transmission facilitates the spread of antimicrobial resistance genes across diverse ecological systems, posing a significant threat to global public health. Since their initial identification in 2006 within the multidrug-resistant plasmid pK245 from , research on the physiological features, epidemiological characteristics, and specific role of IncR plasmids in resistance dissemination has remained limited and fragmented.

Single-cell transcriptomics identifies chondrocyte differentiation dynamics in vivo and in vitro.

Developing in vitro chondrocytes that replicate in vivo development would benefit musculoskeletal disease modeling and regenerative medicine. Although current methodologies have made progress, challenges such as off-target differentiation can result in heterogeneous cell states. Furthermore, the lack of comparison with human embryonic tissue precludes detailed evaluation of in vitro cells.

Molecular insights into how organophosphate esters regulate conventional contaminants removal by Chlorella sorokiniana for synthetic wastewater treatment.

Organophosphate esters (OPEs) are typical types of emerging contaminants that are widely presented in wastewater. While they can serve as phosphorus (P) sources for microalgae growth, their toxicity disrupts metabolic processes, posing challenges to microalgae-based bioremediation. This study elucidated the regulatory effects of OPEs on the removal of ammonia (NH-N) and phosphate (PO-P) from synthetic wastewater by Chlorella sorokiniana, as well as the concurrent OPEs degradation, while unveiling the molecular mechanism of these interactions.

Trends in intestinal aging: From underlying mechanisms to therapeutic strategies.

Intestinal aging is central to systemic aging, characterized by a progressive decline in intestinal structure and function. The core mechanisms involve dysregulation of epithelial cell renewal and gut microbiota dysbiosis. In addition to previous results in model organisms like , recent studies have shown that in mammalian models, aging causes increased intestinal permeability and intestinal-derived systemic inflammation, thereby affecting longevity.

Puerarin attenuates -induced mastitis through inhibiting inflammation and ferroptosis by activating the SIRT1/p53/SLC7A11 signaling pathway.

Mastitis, one of the most prevalent diseases in humans and animals, is commonly caused by local infection of the mammary gland. Puerarin inhibits mitochondrial oxidative stress and acute liver injury in mice. However, the therapeutic effect of puerarin on -induced mastitis is still not well understood.

BCMA-targeting BiTE molecule AMG 420 in relapsed or refractory multiple myeloma: a phase 1b open-label expansion study.

AMG 420 is a first-in-class bispecific T-cell engager (BiTE) molecule directing a cytotoxic T-cell response toward multiple myeloma cells. This phase 1b, open-label, dose-expansion study (NCT03836053) evaluated the safety, tolerability, and efficacy of AMG 420 monotherapy in patients with relapsed/refractory multiple myeloma. Twenty-three patients received continuous intravenous infusion of AMG 420 (200-600 µg/day) in a 6-week cycle.

Genomic epidemiology and anti-biofilm mechanisms of Lactobacillus in ST11 carbapenem-resistant in China.

Background: The ST11 clone of carbapenem-resistant (CRKP) has emerged as a major public health threat, driving hospital outbreaks across China and contributing to chronic infections through robust biofilm formation. The scarcity of effective treatment options poses a critical challenge to clinical management.

Methods: To address this issue, we conducted an integrated genomic epidemiological and metabolomic study of ST11 CRKP isolates collected from 13 hospitals in eastern and central China between 2014 and 2020.

Advanced stability and energy storage capacity in hierarchically engineered BiNaTiO-based multilayer capacitors.

Multilayer ceramic capacitors are cornerstone components of modern electronic systems. Yet ensuring reliability under demanding operational conditions, such as elevated temperatures and prolonged cycling, while achieving holistic optimization of recoverable energy density and efficiency remains a significant challenge. Herein, we implement a polar glass state strategy that catalyzes a profound enhancement in energy storage performance by modulating dynamic and thermodynamic processes.