Genome-wide investigation of transcription factor occupancy and dynamics using cFOOT-seq.

Gene regulation relies on the precise binding of transcription factors (TFs) at regulatory elements, but simultaneously detecting hundreds of TFs on chromatin is challenging. We developed cFOOT-seq, a cytosine deaminase-based TF footprinting assay, for high-resolution, quantitative genome-wide assessment of TF binding in both open and closed chromatin regions, even with small cell numbers. By utilizing the dsDNA deaminase SsdAtox, cFOOT-seq converts accessible cytosines to uracil while preserving genomic integrity, making it compatible with techniques like ATAC-seq for sensitive and cost-effective detection of TF occupancy at single-molecule and single-cell level.

Biomechanical evaluation of the H-Loop technique and single-row knotted technique for repairing upper third subscapularis tendon injuries.

We evaluated the biomechanical performance of the new knotless H-Loop technique in repairing the upper third subscapularis (SSC) tendon tear, and compared these results with the single-row knotted technique. A total of 69 subscapularis tendons from sheep were collected, with 10 specimens randomly selected for comprehensive biomechanical testing after either complete tendon or upper third tear. An additional five specimens were dedicated to measuring the size and extent of the footprint area of the SSC tendon.

Serpina3c Mitigates Adipose Tissue Inflammation by Inhibiting the HIF1α-Mediated Endoplasmic Reticulum Overoxidation in Adipocytes.

Background: Visceral white adipose tissue (vWAT) inflammation is a critical pathology of obesity-caused heart damage and is closely associated with adipocyte endoplasmic reticulum (ER) dysfunction. Serine (or cysteine) peptidase inhibitor, clade A, member 3C (Serpina3c) has been identified as an adipokine with anti-vWAT inflammatory effects. However, it remains unclear whether Serpina3c deficiency promotion of vWAT inflammation involves adipocyte ER dysfunction and whether it further contributes to heart damage in obesity.

Arthroscopic Upper Third Subscapularis Tendon Repair Using H-Loop Technique: A Case Series.

Objective: Arthroscopic repair of upper one-third subscapularis tendon tears remains challenging due to suture management difficulties and repair quality limitations. We proposed a simpler knotless technique-the H-Loop technique. This study evaluates its early clinical and imaging outcomes.

Effects of comprehensive intervention models on cognitive frailty in community-dwelling older adults: A 12-month follow-up study.

Background: Cognitive frailty (CF) is of concern because of its high prevalence in older population and its close association with adverse health outcomes. However, interventions against CF in China are relatively simple. Therefore, the purpose of this study was to analyse the effects of comprehensive interventions on CF in community-dwelling older adults.

A Reverse Suture Anchor Technique for Arthroscopic Medial Meniscus Root Repair.

Injuries of the posterior root of the medial meniscus can be accompanied by damage to the anterior cruciate ligament or often occur independently in cases of degenerative meniscal injury in older individuals. Anchor suture repair can achieve favorable biomechanical effects and clinical outcomes. However, anchor placement is technically challenging and requires a posterior medial approach, which increases the risk of iatrogenic injury.

MicroRNA‑22 regulates autophagy and apoptosis in cisplatin resistance of osteosarcoma.

Osteosarcoma (OS) is a primary malignant tumor of bone tissue. Effective chemotherapy may improve the survival of patients with OS. MicroRNAs (miRs) serve significant roles in the regulatory function of tumorigenesis and chemosensitivity of different types of cancer.

Cognitive impairment after traumatic brain injury is associated with reduced long-term depression of excitatory postsynaptic potential in the rat hippocampal dentate gyrus.

Traumatic brain injury can cause loss of neuronal tissue, remote symptomatic epilepsy, and cognitive deficits. However, the mechanisms underlying the effects of traumatic brain injury are not yet clear. Hippocampal excitability is strongly correlated with cognitive dysfunction and remote symptomatic epilepsy.

Highly enantioselective Michael addition of cyclohexanone to nitroolefins catalyzed by pyrrolidine-based bifunctional benzoylthiourea in water.

Organocatalysis and aqueous reactions are identified as the focus of the greening of chemistry. Combining these two strategies effectively remains an interesting challenge in organic synthesis. Herein, we used pyrrolidine-based benzoylthiourea 1c to catalyze the asymmetric Michael addition of cyclohexanone to various nitroolefins in water to afford the corresponding compounds in moderate to good yields, and with excellent diastereoselectivities (up to >99:1 dr) and enantioselectivities (up to 99% ee).