INTS7 modulates cell proliferation and apoptosis via promoting cell cycle progression in lung adenocarcinoma.

The evolutionarily conserved Integrator complex, which is composed of over 10 subunits, orchestrates diverse RNA-processing events such as 3'-end maturation of small nuclear RNAs (snRNAs), transcription termination of RNA Polymerase II, and DNA damage response signaling pathways; however, the functional roles of individual Integrator complex subunits in lung adenocarcinoma (LUAD) remain poorly characterized, and this study aimed to systematically investigate the potential oncogenic functions and prognostic values of these subunits in LUAD. To achieve this goal, the expression profiles of Integrator complex subunits were profiled using transcriptomic data from the The Cancer Genome Atlas (TCGA) database, survival analyses (including Kaplan-Meier and Cox regression models) were performed to evaluate the correlations between subunit expression levels and patient survival outcomes (overall survival (OS) and disease-free survival (DFS)), co-expression network analysis was conducted to annotate the potential biological functions of key subunits, and functional validation was performed using CCK-8 assays and flow cytometry to assess the impact of INTS7 depletion on cell proliferation and cycle progression in LUAD cell lines. The findings of this study showed that Integrator complex subunits were significantly overexpressed in LUAD tissues compared to normal lung parenchyma; among these subunits, INTS7 expression was most strongly associated with shortened OS and DFS, indicating its pivotal role in LUAD pathogenesis, while bioinformatics analyses revealed that INTS7 is involved in regulating critical biological processes including cell cycle progression, transcriptional regulation, and RNA metabolism, and loss-of-function experiments demonstrated that genetic silencing of INTS7 significantly inhibited cell proliferation and induced cell cycle arrest in LUAD cells.

Recent advances in tumor immunotherapy based on NK cells.

Immunotherapy has emerged as the established fourth pillar of cancer treatment following surgery, radiotherapy, and chemotherapy, representing a cutting-edge research domain in translational medicine and clinical oncology. Natural killer (NK) cells, a type of innate cytotoxic lymphocyte, possess unique antitumor properties that are independent of major histocompatibility complex (MHC) restrictions, making them promising candidates for "off-the-shelf" therapeutic products. NK cells can eliminate tumor cells through various mechanisms.

Analysis of nutritional status and its influencing factors in patients with head and neck cancer: An observational study.

The objective of this study was to explore the short-term changes in nutritional status from preoperative to 2 months postoperatively in head and neck cancer patients, as well as the influencing factors, to provide guidance for clinical interventions. A total of 148 patients with head and neck cancer admitted to the Department of Head and Neck Oncology, Zhejiang Cancer Hospital, were selected. A general questionnaire (demographic data, tumor-related data, body mass index, laboratory indicators) was used to understand the basic information of the patients.

Deep learning-based phase demodulation for distributed acoustic sensor.

With the rapid advancement of deep learning, its applications in fiber optic sensing are expanding significantly, particularly in recognizing diverse scenarios where substantial progress has been made. However, the crucial task of obtaining demodulation results from raw data remains relatively underexplored. Accurate demodulation is essential for a deeper understanding of the physical processes in fiber optic sensing systems, enhancing measurement accuracy, and optimizing system performance.

Low-cost, 4 × 4 pixels, pyroelectric infrared array device based on flexible composites.

The P(VDF-TrFE) and PZT composite materials have the advantages of flexibility, ease of preparation, low cost, and potential for large-scale production. In this paper, a low-cost, 4×4 pixel array-type pyroelectric infrared device based on P(VDF-TrFE)/PZT is prepared. In order to facilitate the interconnection between the sensitive element and readout circuit using the room temperature cured silver paste, the array electrodes are fanned out to the edge of the sensitive element.

Predictive model based on blood cell analysis and coagulation function indicators for neuroblastic tumors staging diagnosis.

Objective: To explore the diagnostic value of integrating blood cell analysis and coagulation function indicators in the staging of neuroblastic tumors, providing a robust basis for clinical decision-making.

Methods: A retrospective analysis was conducted on 137 pediatric neuroblastic tumors cases (2017-2024) at the Children's Hospital Affiliated to Shandong University. Patients were stratified into localized (INSS 1-2, Group 1) and advanced (INSS 3-4, Group 2) stages according to the INSS classification, with mature ganglioneuroma serving as the control group.

The diagnostic of PAX1 gene methylation in cervical lesions and its role in the triage of non-16/18 HR-HPV positive.

Objective: This study aims to systematically evaluate the application value of gene methylation detection in cervical lesion screening and its potential advantages in the triage of non-16/18 high-risk human papillomavirus (HR-HPV) positive patients.

Materials And Methods: This study enrolled 1,619 HPV-positive female patients who visited the Affiliated Hospital of Qingdao University from December 2023 to March 2025, with 989 patients ultimately meeting the inclusion criteria. All subjects underwent HPV-DNA testing, cytological examination, colposcopy, and gene methylation detection, with results analyzed in conjunction with histopathological evaluations.

Luteolin attenuates type 2 inflammation in asthmatic mice induced by OVA by regulating IL-33/ST2- GSK3β-M2 macrophage polarization.

Allergic asthma is a prevalent non-infectious inflammatory disease characterized by type 2 inflammation. Although multiple treatment options are available, their efficacy is often limited due to the heterogeneous nature of asthma. Luteolin (LUT), a naturally occurring flavonoid, has demonstrated therapeutic potential in various inflammatory conditions.

BALs are prognostic biomarkers and correlate with malignant behaviors in breast cancer.

Background: The B-aggressive lymphoma (BAL) proteins, including BAL1, BAL2, and BAL3, constitute a conserved protein family characterized by their N-terminal macro domains and putative C-terminal poly (ADP-ribose) polymerase (PARP) active site. Dysregulation of BALs has been closely associated with the progression of various cancers. However, there is limited understanding of their precise expression profile, prognostic significance, and role in breast cancer (BC).

Resveratrol attenuates hepatic inflammation and oxidative stress in collagen-induced arthritis (CIA) mice via the Nrf2/Keap1 pathway.

Background: Rheumatoid arthritis can affect extra-articular organs such as the liver, and the problem of drug-induced liver injury caused by traditional antirheumatic drugs for improving the condition cannot be ignored. This study aims to investigate the therapeutic effects of resveratrol (Res) on hepatic inflammation and oxidative stress in mice with collagen-induced arthritis (CIA) and to elucidate the relationship between the regulatory mechanisms of the nuclear factor erythroid 2-related factor 2 (Nrf2)/Kelch-like ECH-associated protein 1 (Keap1) signaling pathway.

Methods: In this study, we used chicken type II collagen in combination with complete Freund's adjuvant to induce arthritis in a mouse model, and Res was administered by tube feeding to detect the serum biochemical liver function and inflammation levels, oxidative stress, and apoptosis in the livers of mice.

Prediction of 1-year post-operative mortality in elderly patients with fragility hip fractures in China: evaluation of risk prediction models.

Unlabelled: This study systematically evaluates the predictive capacity of seven risk stratification models for 12-month postoperative mortality in geriatric patients with fragility hip fractures, while concurrently assessing their risk classification accuracy to inform perioperative protocol formulation, rehabilitation strategies, and prognostic management.

Introduction: Current clinical practice lacks standardized criteria for mortality risk prediction in elderly fragility hip fracture patients. This investigation conducts a comparative evaluation of seven prognostic models-the Sernbo Score, Jiang et al.

MoOrp-mediated PtdIns4P transportation is essential for autophagy and pathogenicity in .

Macroautophagy/autophagy is essential to the pathogenicity of . Phosphatidylinositol-4-phosphate (PtdIns4P) is a key lipid involved in the autophagy process. Recent studies have shown that the PtdIns4P pool on autophagic membranes is crucial to autophagosome biogenesis and fusion with the vacuole; however, the mechanism regulating the PtdIns4P levels on autophagic membranes is still unclear.

Analysis of the Causes and Treatment of Surgical Site Infection in Unilateral Biportal Endoscopy for Lumbar Disc Herniation.

Background: Degenerative lumbar spine pathologies are a major cause of chronic low back pain and radiculopathy. In recent years, unilateral biportal endoscopic (UBE) technology has been applied more in the treatment of lumbar disc herniation. However, there are few studies on UBE postoperative infection.

Safety and efficacy of rescue endovascular treatment in ischemic stroke patients with early neurological deterioration caused by large vessel occlusion.

Objectives: The optimal treatment after deterioration following ischemic stroke has not been established in the current guidelines. Therefore, this study evaluated the efficacy of rescue endovascular treatment in patients with early neurological deterioration after acute ischemic stroke.

Methods: This study analyzed data retrospectively retrieved from the electronic medical records at the First Affiliated Hospital of Anhui Medical University.

Estrogen deficiency impairs double-negative T cell function and activates NF-κB in postmenopausal osteoporosis.

Postmenopausal osteoporosis (PMOP), which is driven primarily by estrogen deficiency, is characterized by excessive bone resorption and disrupted immune homeostasis. Although immune system contributions to bone loss are well-documented, the roles of specific regulatory subsets, such as double-negative T (DNT) cells (CD3TCRαβCD4CD8NK1.1), remain unclear.

LncRNA PVT1 promotes cuproptosis through transcriptional activation of FDX1 in colorectal cancer.

Background: Cuproptosis, a copper-dependent form of regulated cell death, has emerged as a redox-sensitive vulnerability in cancer. However, the molecular basis by which this process is initiated and sustained in tumors remains poorly defined.

Methods: We investigated the functional role of FDX1 in cuproptosis in colorectal cancer through a series of in vivo and in vitro assays.

Baseline type 2 biomarker levels and clinical remission predictors in children with asthma.

Background: Few studies have investigated the relationship between baseline type 2 biomarker levels and clinical features in pediatric asthma, particularly in different asthma stages, which may inform prognosis and remission.

Objective: To explore the association between baseline Th2 biomarker levels and clinical manifestations in pediatric asthma, identifying predictors of clinical remission.

Methods: The study included 172 children with a mean age of 6.

Impact of 16/8 time-restricted eating on body composition and lipolytic hormone regulation in female DanceSport dancers.

Objective: The aim of this study was to investigate the effect of a 16/8 time-restricted eating (TRE) program over 6 weeks on body composition and lipolytic hormone levels in female DanceSport dancers. Importantly, participants were not subject to any calorie restrictions during the study period.

Methods: A total of 20 female DanceSport dancers were recruited to participate in the randomized controlled trial.

Perception of native vs. non-native language and non-speech sounds in one-week-old neonates: An fNIRS study.

Background: Early infant phonological and non-phonological perceptual abilities are crucial for future language learning. Previous studies have focused on the changes in the cerebral cortex of infants and toddlers during speech perception, while the changes in the cerebral cortex during non-speech perception remain poorly understood.

Objective: This study aimed to investigate cortical activation patterns and differences in full-term healthy newborns under different speech and non-speech stimuli by functional near-infrared spectroscopy (fNIRS).

Selection and identification of the ssDNA aptamer against polymyxin B sulfate.

Background: Polymyxin B (PMB) is a potent antibiotic, and its sulfate form, Polymyxin B Sulfate (PMBS), is widely used. When PMBS is used as a veterinary drug, excessive use can lead to drug residues in animal bodies, which is a significant food safety issue and raises serious concerns. Therefore, there is an urgent need to strengthen research in this area.

Transgenic human nephrin in Drosophila nephrocytes facilitates variant analysis.

Introduction: Nephrin, the key structural protein of the slit diaphragm, is encoded by NPHS1. Pathogenic variants in this gene are the primary cause of congenital nephrotic syndrome. About 400 variants have been described but functional characterization in vitro is very limited.

Specific Detection of RHDV GI.1 and GI.2 by RT-LAMP-CRISPR/Cas12a Platform.

Rabbit hemorrhagic disease is a highly contagious and acute fatal disease caused by rabbit hemorrhagic disease virus (RHDV). The first outbreak of RHDV2 in 2020 has posed a serious threat to the rabbit breeding industry in China. An effective and specific detection strategy for RHDV GI.

Interaction of polymorphism with methylation in cervical cancer.

We aimed to investigate the roles and interaction effects of high-risk human papillomavirus (HR-HPV) infection, methyltetrahydrofolate reductase polymorphism, and paired box gene 1 () methylation in cervical intraepithelial neoplasia (CIN) and cervical cancer. Polymerase chain reaction was used to detect polymorphism and methylation; Mantel-Haenszel and Spearman's rank correlation tests were used to analyze the trends and correlations. Forty cases each of normal control (NC), CIN I, and CIN II/III and 9 squamous cell carcinoma (SCC) cases were enrolled.

The prognostic value of growth pattern-based grading for mucinous ovarian carcinoma (MOC): a systematic review and meta-analysis.

Objective: To investigate the prognostic significance of expansile and infiltrative growth patterns in mucinous ovarian carcinoma (MOC).

Methods: A systematic search was conducted in the PubMed, Embase, and Web of Science databases for studies published between January 1, 2010, and September 6, 2024, examining the correlation between expansile and infiltrative tumor growth patterns and prognosis in MOC. Subgroup analyses were performed for mortality, recurrence, and FIGO stage I based on tumor subtype.

Long-Lasting Hydrogen Evolution and Efficient Dew Harvest Realized via Electrospinning Polyvinylidene Fluoride Membrane on Hybrid Hydrogels.

Long-lasting hydrogen evolution and efficient dew harvest is realized via electrospinning a polyvinylidene fluoride (PVDF) membrane on hybrid hydrogels embedded with photocatalytic g-CN/Pt nanosheets. Due to the hindrance of water evaporation by the hydrophobic PVDF membrane, the drying process of the hybrid hydrogels significantly slows down. Hence, the g-CN/Pt nanosheets can continue working on photocatalytic splitting of the water molecules in the hydrogels.