The diagnostic of PAX1 gene methylation in cervical lesions and its role in the triage of non-16/18 HR-HPV positive.

Objective: This study aims to systematically evaluate the application value of gene methylation detection in cervical lesion screening and its potential advantages in the triage of non-16/18 high-risk human papillomavirus (HR-HPV) positive patients.

Materials And Methods: This study enrolled 1,619 HPV-positive female patients who visited the Affiliated Hospital of Qingdao University from December 2023 to March 2025, with 989 patients ultimately meeting the inclusion criteria. All subjects underwent HPV-DNA testing, cytological examination, colposcopy, and gene methylation detection, with results analyzed in conjunction with histopathological evaluations.

Interaction of polymorphism with methylation in cervical cancer.

We aimed to investigate the roles and interaction effects of high-risk human papillomavirus (HR-HPV) infection, methyltetrahydrofolate reductase polymorphism, and paired box gene 1 () methylation in cervical intraepithelial neoplasia (CIN) and cervical cancer. Polymerase chain reaction was used to detect polymorphism and methylation; Mantel-Haenszel and Spearman's rank correlation tests were used to analyze the trends and correlations. Forty cases each of normal control (NC), CIN I, and CIN II/III and 9 squamous cell carcinoma (SCC) cases were enrolled.