Rapid and simple detection of using real closed dumbbell-mediated isothermal amplification.

Introduction: () is a well-known widespread food-borne pathogen that poses a threat to public health. Suitable detection methods are needed to effectively control and prevent pathogenic infections.

Methods: This study aimed to develop a novel closed dumbbell-mediated isothermal amplification (CDA)-based assay to achieve rapid and simple detection of The newly developed CDA technology is capable of amplifying DNA targets with high sensitivity and specificity.

Three undescribed Schinortriterpenoids from Schisandra chinensis and their neuroprotective effects.

Three undescribed SNTs, schisaterpene SU (1-3), were isolated from the extract of Schisandra chinensis. Their structures were elucidated on the basis of HR-ESI-MS, NMR data and ECD calculation. All compounds were evaluated for their Neuroprotective activity induced by MPP in SH-SY5Y cells.

Improved modelling of biogenic emissions in human-disturbed forest edges and urban areas.

Biogenic volatile organic compounds (BVOCs) are critical to biosphere-atmosphere interactions, profoundly influencing atmospheric chemistry, air quality and climate, yet accurately estimating their emissions across diverse ecosystems remains challenging. Here we introduce GEE-MEGAN, a cloud-native extension of the widely used MEGAN2.1 model, integrating dynamic satellite-derived land cover and vegetation within Google Earth Engine to produce near-real-time BVOC emissions at 10-30 m resolution, enabling fine-scale tracking of emissions in rapidly changing environments.

Secondary organic aerosol in urban China: A distinct chemical regime for air pollution studies.

In the past decades, China has witnessed high air pollution associated with rapid economic development, although regulatory efforts have alleviated the situation since 2013. Haze events characterized by high particulate matter (PM) levels in China are not only of enormous magnitude but also represent a distinct chemical regime. Once driven by direct emissions, these high-PM episodes are now more affected by secondary aerosol, especially secondary organic aerosol (SOA).

Selection at the locus in horses and its implications for human mobility.

Horsepower revolutionized human history through enhanced mobility, transport, and warfare. However, the suite of biological traits that reshaped horses during domestication remains unclear. We scanned an extensive horse genome time series for selection signatures at 266 markers associated with key traits.

S-9-PAHSA ameliorates cognitive decline in a type 2 diabetes mouse model by inhibiting oxidative stress and apoptosis via CAIII modulation.

Purpose: S-palmitic acid-9-hydroxy stearic acid (SP), a newly characterized endogenous lipid with multifaceted biological activities, is poised to shed light on its potential in diabetes-related cognitive disorder (DRCD). This study aims to uncover the effects of SP on DRCD and the underlying mechanisms.

Methods: C57BL/6 mice were fed with high-fat diet for 5 months to induce type 2 diabetes mellitus (T2DM).

Pain in the body, harm to the heart: advances in research on the impact of chronic pain on cardiovascular diseases.

Chronic pain (CP) is highly prevalent, and a substantial proportion of patients concurrently suffer from cardiovascular diseases (CVD), suggesting a complex interplay between these two conditions. CP increases the risk of CVD through multiple mechanisms, including sympathetic overactivation, neuroimmune inflammatory responses, endocrine and metabolic dysregulation, and bidirectional regulation along the heart-brain axis. Moreover, pharmacological treatments traditionally used for CP, such as non-steroidal anti-inflammatory drugs (NSAIDs), opioids and related agents, are associated with heightened cardiovascular risks.

MY-1 promotes angiogenesis in the ischemic hindlimbs by regulating the stability of CDC42 via PSMD14.

Critical limb ischemia (CLI) is a refractory peripheral artery disease characterized by tissue ischemia, presenting significant therapeutic challenges. Current surgical revascularization treatments are limited by indications, complications, and other constraints, making the identification of novel therapeutic strategies an important objective for CLI management. In this study, we designed and synthesized a novel short peptide, named MY-1, and developed a GelMA/MY-1 hydrogel sustained-release system for local application in a mouse hindlimb ischemia model.

Treatment-free remission in chronic myeloid leukaemia patients with accelerated phase or tyrosine kinase inhibitor therapy failure.

We studied 60 chronic myeloid leukaemia (CML) patients with a prior history of accelerated phase (AP) including de novo AP (n = 19) and transformation to AP (n = 4), or tyrosine kinase inhibitor (TKI) therapy failure in the chronic phase (CP, n = 37), who discontinued TKI therapy. Median interval from diagnosis with AP or TKI therapy failure to achieving a deep molecular response (DMR) was 19 months (interquartile range [IQR], 9-30 months). Median TKI treatment and DMR duration were 108 months (IQR, 72-137 months) and 59 months (IQR, 39-87 months) respectively.

Structural-Functional Characterization and Bioactive Potential of Alternative Grain Proteins: An In-Depth Comparison of Quinoa and Oat Proteins Isolates.

The growing preference for plant-based proteins has led to increased interest in quinoa and oat as vital sources of plant-derived proteins. This study conducted a comprehensive characterization of two quinoa protein isolates (QJP1 and QJP3) and two oat protein isolates (OBP3 and OPP4). Results showed that quinoa protein, particularly QJP1, was found to be rich in lysine and, compared with oat protein isolates, exhibited potent antioxidant properties, making it an ideal choice for vegetarians, vegans, and the elderly who require plant-based sources of essential amino acids and antioxidants.

[Construction of a activation vector based on the CRISPR/dCas9 system and its validation in sheep embryonic stem cells].

The CRISPR/dCas9 system is a gene editing tool that has proven to be highly efficient and precise. By utilizing transcriptional activators, such as VP64, p65, and Rta, the system can effectively and stably activate target genes. Sox17, a transcription factor belonging to the SOX family, plays a crucial role in the differentiation of the germ layers and the determination of cell fates during the early stages of embryonic development.

Zearalenone Regulates Energy Metabolism and Proliferation of Goat Endometrial Epithelial Cells through Estrogen Receptor 1.

Zearalenone (ZEN), an estrogenic mycotoxin inducing reproductive toxicity, disrupts endometrial cell function and contributes to pregnancy failure, although its endometrial toxicity mechanisms remain unclear. This study reveals that ZEN inhibits goat endometrial epithelial cells (EECs) proliferation through estrogen receptor 1 (ESR1) homodimers. ESR1 knockout reversed ZEN-induced mitochondrial structural/functional damage via the ESR1-OMA1 zinc metallopeptidase (OMA1) axis.

Association between serum gamma-glutamyl transferase levels and polyvascular atherosclerotic plaques and stenosis: A cross-sectional study.

Introduction Serum gamma-glutamyl transferase (GGT) was associated with cardiovascular disease. However, limited research has explored the relationship between GGT and polyvascular atherosclerosis. Methods This study is based on the baselines cross-sectional survey of the PolyvasculaR Evaluation for Cognitive Impairment and vaScular Events study, a population-based cohort study.

Impact of Nonpharmaceutical Interventions During the COVID-19 Pandemic on the Epidemiology and Seasonal Patterns of Acute Respiratory Infections in Children.

This study investigates the effects of non-pharmaceutical interventions (NPIs) on the viral spectrum and seasonal dynamics of acute respiratory tract infections (ARTIs) in children, with a particular focus on post-NPI data. The analysis compares viral detection rates and seasonal patterns post-NPI with those observed during the pre-NPI and NPI periods, as previously published. We performed a retrospective analysis of 93,852 pediatric cases of ARTIs from 2018 to 2024 at the Third Affiliated Hospital of Zhengzhou University.

Nanotherapies based on bacterial metabolism: Mechanisms, design and application.

Bacterial metabolism provides the essential materials and energy for growth and reproduction. Through complex transformations and electron transfer, bacteria convert external nutrients into useable forms and achieve pathogenicity in the human body. In recent years, with the global spread of antibiotic resistance, traditional antibiotic treatments have become increasingly ineffective and may even exacerbate the imbalance of the human microbiota.

Characterization of Soil Bacterial Communities in Different Vegetation Types on the Lava Plateau of Jingpo Lake.

To explore the interactions within the vegetation-soil-microorganism continuum on the Jingpo Lake lava platform, five vegetation types-grassland (GL), shrubland (SL), deciduous broad-leaved forest (DB), coniferous and broad-leaved mixed forest (CB), and coniferous forest (CF)-were examined. Significant differences in the soil physical and chemical properties were identified among these types ( < 0.05).

Trees use exogenous sugars for growth, but excess triggers negative feedback reducing photosynthetic carbon gain.

Plants' non structural carbohydrates (NSCs) serve as their capital for growth, reproduction, defense and survival. To increase the NSC availability of carbon-limited trees, a recent study revealed the possibility of adding exogenous soluble sugars to carbon-starved trees. This provides an opportunity to investigate carbon allocation between source and sink, as well as the growth and physiological responses to external sugars.

Metal-Organic Frameworks Nanocomposite Enzymes with Peroxidase-like Activity: Can They Bring Us a New Perspective in the Field of Biological Applications?

This review aims to systematically investigate the structure, function, and practical applications of metal-organic framework (MOF) nanocomposite enzymes with peroxidase-like activity. MOFs are a class of porous crystalline materials formed by metal nodes and organic ligands, exhibiting enzyme-like activity due to their structural similarity to natural enzymes. They have garnered significant attention in analytical chemistry, disease diagnosis, and treatment.

The m6A reader IGF2BP3 facilitates myogenesis by activating the CAMK2B-MEF2C axis.

Skeletal muscle development directly impacts meat quality and productivity in livestock. Although N6-methyladenosine (m6A) RNA methylation has been recognized as a crucial epigenetic mechanism governing myogenesis, the specific functions of Insulin-like Growth Factor 2 mRNA-binding Protein 3 (IGF2BP3), a key m6A reader protein, in goat muscle development remain elusive. This study was designed to dissect the role of IGF2BP3 and its m6A-mediated regulatory mechanisms during goat skeletal muscle development.

Localization and ambiguity resolution algorithm for time-difference fusion of three satellites based on observation filtering.

To address the issues of low accuracy and time-difference ambiguity in the localization and tracking of multiple satellites, we have conducted a thorough study of the localization algorithm and the ambiguity resolution algorithm for the time-difference fusion of multiple satellites. The localization algorithm based on Gaussian-Newton iteration for time-difference fusion of three satellites is proposed. The time-difference equation observed during the satellite overhead is combined with the elevation observation equation to construct the cost function for the overdetermined case, and the nonlinear least squares problem is solved based on Gaussian-Newton iteration.

Dual Antiplatelet Therapy in Patients With Metabolic Syndrome After Mild Ischemic Stroke or Transient Ischemic Attack.

Background: Metabolic syndrome (MetS) attenuates antiplatelet agent effects. This study investigated the efficacy and safety of clopidogrel-aspirin therapy for secondary stroke prevention in patients with MetS.

Methods: Data were obtained from the INSPIRES (Intensive Statin and Antiplatelet Therapy for Acute High-Risk Intracranial or Extracranial Atherosclerosis) trial.

Characteristics of gastric fluid microbiota in patients with refractory infection.

Introduction: Refractory infection (RHPI) poses a clinical challenge due to its treatment resistance. The gastric microbiota characteristics in patients with RHPI remain unclear. This study analyzed gastric fluid samples to explore the structural and functional differences in the gastric microbiota of patients with RHPI and their association with clinicopathological features, providing a theoretical basis for precision treatment of RHPI.

Photoreceptor-dependent signaling in plant responses to thermomorphogenesis and heat stress.

Climate change and increasingly frequent extreme weather events are profoundly disrupting ecosystems and agricultural production. As essential components in plants, photoreceptors play a pivotal role in mediating responses to environmental stimuli, including light and temperature, and thus a deeper understanding of these mechanisms may aid efforts to mitigate the impacts of climate change. Recent studies have revealed intricate signaling networks and molecular mechanisms underlying photoreceptor-mediated temperature perception and response.

Case report: A case of acute pancreatitis with myocarditis and thyrotoxicosis.

Background: A variety of diseases can complicate acute pancreatitis and myocarditis. As acute pancreatitis combined with myocarditis and thyrotoxicosis is rare, we report a case of acute pancreatitis combined with myocarditis and thyrotoxicosis.

Case Presentation: We present a case of a previously healthy 37-year-old male who was admitted with epigastric pain; elevated blood amylase, lipase, and cardiac enzymes; markedly abnormal thyroid function; and an electrocardiogram showing elevated ST segments.