[Analysis of gene expression in synovial fluid and blood of patients with knee osteoarthritis of Yang deficiency and blood stasis type].

Objective: To reveal the molecular basis of knee osteoarthritis (KOA) with Yang deficiency and blood stasis syndrome by analyzing the gene expression profiles in synovial fluid and blood of KOA patients with this syndrome.

Methods: A total of 80 KOA patients were recruited from October 2022 to June 2024, including 40 cases in the non- deficiency and blood stasis group (27 males and 13 females), with an average age of (61.75±3.

Photo-triggered engineered microbes achieving dual epigenetic immunomodulation for gastric cancer.

DNA methylation serves as an essential epigenetic mechanism for the regulation of gene expression. One of the earliest and most common changes seen in cancer is abnormal DNA methylation at CpG sites, which may encourage tumor development by silencing tumor suppressor gene transcription. The DNMT1 inhibitor AZA can delay cancer progression by inhibiting DNA methylation and enhancing tumor immunogenicity, yet its clinical efficacy in gastric cancer is limited by poor specificity, instability, and low retention.

Crosstalk between macrophages and fibroblasts contributes to inflammation and damage in giant cell arteritis.

Objectives: Giant cell arteritis (GCA) is a large vessel vasculitis characterized by arterial wall inflammation and remodeling. Macrophages and fibroblasts are abundantly present in arteries affected by GCA, but their crosstalk in GCA pathogenesis is largely unknown. Here we investigated the interaction between macrophages and fibroblasts in GCA-affected arteries and in vitro.

Association between serum gamma-glutamyl transferase levels and polyvascular atherosclerotic plaques and stenosis: A cross-sectional study.

Introduction Serum gamma-glutamyl transferase (GGT) was associated with cardiovascular disease. However, limited research has explored the relationship between GGT and polyvascular atherosclerosis. Methods This study is based on the baselines cross-sectional survey of the PolyvasculaR Evaluation for Cognitive Impairment and vaScular Events study, a population-based cohort study.

Ni-Bis(dithiolene) Coordination Enhanced Dual-Functional Covalent Organic Frameworks for both Cathodic Zn Storage and Anodic Zinc Deposition Control in Aqueous Zn-Ion Batteries.

In response to the increasing demand for sustainable energy storage solutions, aqueous zinc-ion batteries (AZIBs) have garnered significant attention for their high safety, low cost, and environmental friendliness. However, two major challenges to battery stability persist: developing efficient cathode materials and addressing zinc dendrite formation. Here, we report a dual-functional covalent organic framework (COF), named Ni-DAPTO, designed to improve both Zn storage on the cathode and zinc deposition kinetics on the anode.

Mechanisms Underlying Radioresistance and Reversal Strategies in Non-Small Cell Lung Cancer.

Radiotherapy (RT) continues to be a fundamental component in the management of non-small cell lung cancer (NSCLC). Nevertheless, some NSCLC patients do not attain optimal therapeutic outcomes due to the emergence of radioresistance. Improving the effectiveness of RT in NSCLC necessitates a thorough comprehension of the mechanisms that lead to radioresistance.

Lauric Acid Promotes Esophageal Squamous Cell Carcinoma via GPR84: Lipid Metabolic Dysregulation as a Therapeutic Target.

Various studies have demonstrated metabolic disorders in patients with esophageal squamous cell carcinoma (ESCC), although their potential role in ESCC development remains unclear. Here, we investigated alterations in the serum and fecal metabolomes of 23 ESCC patients and 23 healthy controls using untargeted metabolomics. Further analysis identified lauric acid as a biomarker for ESCC through targeted metabolomics, with validation in 88 ESCC patients and 44 healthy controls.

Comparative efficacy of exercise modalities on sleep architecture in adults with sleep disorders: a systematic review and network meta-analysis of randomized controlled trials.

Objective: This study aims to evaluate the efficacy of exercise interventions on sleep architecture in individuals with sleep disorders and to determine the optimal exercise modalities.

Methods: A comprehensive search of PubMed, Embase, EBSCO, Scopus, PsycINFO, and Web of Science was performed for randomized controlled trials (RCTs) published up to March 2025. Meta-analyses and Bayesian network meta-analyses (NMA) were performed using a random-effects model, with mean differences (MDs) as the effect size measure.

Community-Based Intelligent Blood Glucose Management for Older Adults With Type 2 Diabetes Based on the Health Belief Model: Randomized Controlled Trial.

Background: The number of older patients with type 2 diabetes (T2D) is increasing, and effective self-management is crucial for controlling disease progression and its complications.

Objective: We designed a home telemedicine intervention that combines telemedicine with health education based on the Health Belief Model (HBM). This study evaluated its effectiveness on self-management in older patients with T2D.

Cancer cell-derived migrasomes harboring ATF6 promote breast cancer brain metastasis endoplasmic reticulum stress-mediated disruption of the blood-brain barrier.

Objective: Migrasomes, an emerging class of migration-facilitating membranous extracellular vesicles, remain largely uncharted in the intricate landscape of tumor metastasis. This study aimed to illuminate the roles and mechanisms underlying cancer cell-derived migrasomes in breast cancer brain metastasis (BCBM).

Methods: Migrasomes were isolated and purified from BCBM cells (231-BR) and non-specific organotropic parental counterparts (MDA-MB-231), specifically designated as Mig-BCBM and Mig-BC, respectively.

Spatial discovery of pyrotinib overcoming HER2-positive breast cancer resistance by breaking fibroblast-induced immune barriers.

Background: The TCbHP regimen, consisting of combining docetaxel (T), carboplatin (Cb), trastuzumab (H), and pertuzumab (P), is the preferred neoadjuvant treatment for locally advanced human epidermal growth factor 2 (HER2)-positive breast cancer. However, about 40 % of patients develop resistance to this treatment. Adding TKIs like pyrotinib to anti-HER2 antibodies may enhance efficacy and reduce resistance, but the mechanisms are not fully understood.

Monocyte membrane-coated drug nanocrystals for enhanced targeted therapy of rheumatoid arthritis.

Rheumatoid arthritis (RA) is a chronic autoimmune disorder with increasing global prevalence. Despite substantial research efforts, the exact etiology of RA remains elusive, and its pathological environment involved appears complicated. To date first-line RA therapies primarily rely on pharmacological interventions, involving chemical and biologic agents that exert systemic immunosuppressive effects or inhibit single signaling molecule blockage.

Prediction of the fertile window and menstruation with a wearable device via machine-learning algorithms.

Research Question: Can algorithms for prediction of the fertile window and menstruation be developed through machine learning based on women's physiological parameters collected using a wearable device (Huawei Band 6 Pro) from both regular and irregular menstruators?

Design: This prospective observational cohort study was conducted at the Obstetrics and Gynaecology Hospital of Fudan University (China). Participants were recruited from November 2021 to September 2022. Each participant used a wearable device to record their wrist skin temperature (WST), heart rate, heart rate variability and respiratory rate for two menstrual cycles.

regulation of non-small cell lung cancer radiotherapy resistance through the pathway of protective mitophagy.

Background: Radiotherapy resistance has become one of the major causes of radiotherapy failure among patients with non-small cell lung cancer (NSCLC), but its underlying mechanism remains unclear. In recent years, the influence of mitochondrial autophagy on the radiotherapy resistance in treated tumor cells and its regulatory mechanism has become a hotspot in research, which is also the subject of our group research effort. The primary objective of our study is to investigate the mitophagy-associated pathway and the regulatory mechanisms underlying radiotherapy resistance in NSCLC.

DLL4/VEGF bispecific molecularly imprinted nanomissile for robust tumor therapy.

Tumor-induced angiogenesis plays a pivotal role in the progression and expansion of solid tumors, making anti-angiogenic therapies a promising strategy in cancer treatment. However, compensatory angiogenesis, which can drive drug resistance and tumor recurrence, poses significant challenges in anti-angiogenic therapy. Therefore, improved anti-tumor angiogenesis therapy has become a critical necessity.

Regional Hypoperfusion Predicts White Matter Tract Degeneration in Recent Single Subcortical Infarcts.

Background: The association between baseline cerebral perfusion status and perilesional white matter degeneration in recent single subcortical infarcts (RSSIs) is still not well understood. We aimed to investigate the clinical relevance and possible predictors of long-term morphological changes of RSSIs.

Methods: Patients with RSSI who had baseline computed tomography perfusion and at least 1 follow-up magnetic resonance imaging were retrospectively enrolled from the stroke registry databases.

Phosphoantigen-induced inside-out stabilization of butyrophilin receptor complexes drives dimerization-dependent γδ TCR activation.

Phosphoantigens (pAgs), produced by infected or cancer cells, trigger the assembly of a membrane receptor complex comprising butyrophilin (BTN) members BTN3A1 and BTN2A1, leading to the activation of γδ T cells. BTN3A2 or BTN3A3 forms heteromers with BTN3A1, exhibiting higher γδ T cell receptor (TCR)-stimulating activity than BTN3A1 homomers. Cryoelectron microscopy (cryo-EM) structure reveals a pAg-induced BTN2A1-BTN3A1 heterotetramer with a 2:2 stoichiometry, stabilized by interactions between the intracellular B30.

TEX10: A Novel Drug Target and Potential Therapeutic Direction for Sleep Apnea Syndrome.

Background: Sleep apnea syndrome (SAS) is a prevalent sleep disorder strongly associated with obesity, metabolic dysregulation, and cardiovascular diseases. While its underlying pathophysiological mechanisms remain incompletely understood, genetic factors likely play a pivotal role in SAS pathogenesis. This study investigates the causal relationships between potential drug target genes and SAS using multiple statistical approaches, aiming to provide novel insights for targeted therapeutic development.

Functional characterization of TaWRKY254 in salt tolerance based on genome-wide analysis of the WRKY gene family in wheat core parent Zhou8425B.

The WRKY gene family plays a pivotal role in regulating plant growth, development, and stress responses. Zhou8425B, a core wheat parent in Chinese breeding programs known for its superior agronomic traits, remains underexplored in terms of its WRKY functional landscape. In this study, we identified 294 WRKY transcription factors in the Zhou8425B genome and conducted comprehensive bioinformatics analyses covering gene structure, protein properties, phylogenetic relationships, conserved motifs, and cis-regulatory elements.

Acid-sensing ion channel 1a promotes LPS-induced acute lung injury through the circRNA 18-658/miR-127-5p/TRIM72 axis.

Acute lung injury (ALI) is a serious disease with sudden onset, rapid progression, poor treatment response and high mortality. Acid-sensitive ion channels (ASICs) are a type of cation channel activated by extracellular acidification and are involved in the pathogenesis of inflammatory and immune diseases. Our previous research revealed that ASIC1a promotes ALI, but the specific mechanism is unclear.

Urolithin A alleviates radiation pneumonitis by activating PINK1/PRKN-mediated Mitophagy.

Background: Radiation pneumonitis (RP) is a common and severe complication of radiotherapy, whose pathogenesis involves complex inflammatory responses and cellular damage. Despite its clinical significance, effective treatments remain limited. This study investigates the role of radiation-induced PINK1/PRKN-mediated mitophagy and type I interferon responses in RP and evaluates the therapeutic potential of Urolithin A (UA) in regulating inflammation through mitophagy activation.

A diagnostic model for non-invasive urothelial cancer early detection based on methylation of urinary tumor DNA.

Background: Diagnostic methods for urothelial cancer (UC) are often invasive, while urinary cytology, a non-invasive alternative, suffers from limited sensitivity. This study aimed to identify differentially methylated markers in urinary tumor DNA and develop a diagnostic method to enhance the sensitivity of non-invasive UC detection.

Methods: Whole-genome bisulfite sequencing and deep methylation sequencing were employed to identify significantly hypermethylated UC-associated genes in clinical samples and public UC datasets.

Optimal visual search with highly heuristic decision rules.

Visual search is a fundamental natural task for humans and other animals. We investigated the decision processes that humans use in covert (single fixation) search with briefly presented displays having well-separated potential target locations. Performance was compared with the Bayesian-optimal decision process under the assumption that the information from the different potential target locations is statistically independent.

Effects of SGLT2 inhibitors and GLP-1 receptor agonists on glycemic variability, islet cell function, and insulin resistance in patients with type 2 diabetes mellitus and renal cell carcinoma.

This study evaluated the effects of sodium-glucose co-transporter 2 inhibitors (SGLT2i) and glucagon-like peptide-1 receptor agonists (GLP-1RA) on glycemic control, islet cell function, and insulin resistance in type 2 diabetes mellitus (T2DM) patients with T1-stage renal cell carcinoma (RCC). A retrospective cohort of 175 patients was divided into a control group receiving SGLT2i monotherapy (n = 84) and an observation group receiving combination therapy with SGLT2i and GLP-1RA (n = 91). Propensity score matching (PSM) was employed to balance baseline characteristics, resulting in 35 patients per group.