Traction-regulated persistence governs durotaxis across cell types.

Cell migration toward stiffer or softer environments (durotaxis) underlies processes from development to cancer metastasis, yet the underlying mechanism and its universality remain unclear. To resolve this, we investigated how traction forces and directional persistence dictate cell migration along stiffness gradients. We utilized tunable PEG hydrogels with stiffness gradients of 1-16 kPa and perturbed contractility (blebbistatin, oligomycin), and adhesion (vinculin mutants), in cancer cells exhibiting opposing durotactic biases.

Tuning Interfacial Thermal Transport through Phase Engineering in 2D Ferroelectrics.

As a 2D material with distinctive ferroelectric properties, InSe offers significant potential for the applications in information memory and advanced data storage technologies. It also exhibits a complex phase diagram that is highly sensitive to temperature and pressure variations, resulting in diverse lattice configurations. While extensive studies have focused on the phase transition behavior of InSe, its impact on phonon transport remains largely unexplored.

Discovery of novel 1,2,4-triazole schiff bases hybridized with 1,4-naphthoquinones as potent STAT3 inhibitors.

Signal transducer and activator of transcription 3 (STAT3) is closely related to a variety of cancers. The research of novel STAT3 inhibitors is an important and meaningful project for pharmaceutical chemist. In this study, 10 novel 1,4-naphthoquinones carrying 1,2,4-triazole derivatives were synthesized via a four-step synthesis starting from 2-hydroxynaphthalene-1,4-dione.

Impact of prehospital delay on postoperative complications and 5-year mortality in older adults with hip fractures.

Purpose: Guidelines recommended early surgery for hip fracture to improve outcomes, yet it is often hindered by prehospital delays. However, it remains unclear whether prehospital delay independently leads to poor outcomes of the well-recognized impact of in-hospital delay for hip fracture surgery.

Methods: We included patients aged over 60 years old who underwent surgery for their first acute hip fracture between 2000 and 2022 at a national trauma center in Beijing, China.

Rational design of a bispecific antibody for rapid and prolonged neutrophil mobilization and proliferation.

Human granulocyte colony stimulating factor (G-CSF) and the G-protein C-X-C chemokine receptor 4 (CXCR4)/stromal cell-derived factor-1 (SDF-1) axis play pivotal roles in neutrophil mobilization, differentiation and proliferation. Bispecific antibodies represent a viable therapeutic strategy by simultaneously targeting these two granulocyte-associated receptors while providing an extended half-life. In this study, we engineered a bispecific antibody by grafting human G-CSF and a synthetic anti-CXCR4 peptide into the heavy chain complementarity-determining region 3 (CDR3) of the humanized antibody Herceptin.

The IL-36γ/PEDF/PPARγ signalling pathway plays an anti-inflammatory role in Candida albicans keratitis.

Fungal keratitis caused by Candida albicans (CA) is a common, disabling eye disease with a complex immune response system, affecting diagnosis, treatment, and prognosis. The specific regulatory roles and interactions of IL-36γ and pigment epithelium-derived factor (PEDF) in this disease remain largely unclarified. Additionally, the influence of miR-204-5p on the expression and anti-inflammatory functions of IL-36γ and PEDF in CA keratitis is insufficiently explored.

scIVNL-seq resolves in vivo single-cell RNA dynamics of immune cells during Salmonella infection.

The immune response against pathogens involves multiple cell state transitions and complex gene expression changes. Here, we establish a single-cell in vivo new RNA labeling sequencing method (scIVNL-seq) and apply it to survey time-resolved RNA dynamics during immune response to acute enteric infection with Salmonella. We show that the detection of new RNA synthesis reflects more realistic information on cell activation and gene transcription than total RNA level.

Snord15b Maintains Stemness of Intestinal Stem Cells via Enhancement of Alternative Splicing of Btrc Short Isoform for Suppression of β-Catenin Ubiquitination.

Intestinal epithelium is derived from Lgr5 intestinal stem cells (ISCs) located at the crypt base. However, how small nucleolar RNAs (snoRNAs) regulate ISC stemness remains elusive. Here, a conserved snoRNA, Snord15b, that is highly expressed in ISCs is identified.

Novel Zwitterionic Hydrogels with High and Tunable Toughness for Anti-Fouling Application.

Zwitterionic hydrogels have emerged as eco-friendly anti-fouling materials owing to their superior hydration-mediated resistance to biofouling. Nevertheless, their practical utility remains constrained by intrinsically poor mechanical robustness. Herein, this study proposes a novel strategy to develop novel tough zwitterionic hydrogels by freezing the gels' polymer network.

The Significant Impact of Biomass Burning Emitted Particles on Typical Haze Pollution in Changsha, China.

In this study, typical haze pollution influenced by biomass burning (BB) activities in Changsha in the autumn of 2024 was investigated through the mixing state and evolution process of BB particles via the real-time measurement of single-particle aerosol mass spectrometry (SPAMS). From the clean period to the haze period, the PM concentration increased from 25 μg·m at 12:00 to 273 μg·m at 21:00 on 12 October, and the proportion of total BB single particles in the total detected particles increased from 17.2% to 54%.

Gut microbiota and diabetic neuropathy/peripheral artery disease: A two-sample Mendelian randomization study investigating risk factors for diabetic foot ulcers.

Diabetic foot ulcers (DFUs) represent a significant complication affecting the lower extremities in individuals with diabetes. The development and progression of DFUs are primarily influenced by diabetic neuropathy (DN) and diabetic peripheral artery disease (DPAD). Recent studies indicate a novel link between the gut microbiota and these risk factors.

Breast cancer as the first primary malignancy: clinical pathological characteristics of common cancer combinations.

With the continuous rise in breast cancer incidence and significant improvement in patient survival, the risk of developing Multiple Primary Malignancies (MPMs) has garnered increasing attention. As the most common malignant tumor in women, breast cancer has a complex pathogenesis involving multiple factors such as genetic predisposition, treatment exposure, and interactions between hormonal pathways. These tumors are often difficult to distinguish from metastases or recurrences in clinical practice, frequently leading to delays in diagnosis and treatment, thereby affecting patient prognosis.

Microbiota metabolite taurodeoxycholic acid maintains intestinal tissue residency of innate lymphoid cells via engagement with P2Y10 receptor.

Innate lymphoid cells (ILCs) play critical roles in innate immunity, epithelial barrier protection, and tissue homeostasis. However, the maintenance machinery of intestinal tissue residency of ILCs remains elusive. Here, we show that gut microbiota is necessary for the maintenance of intestinal tissue residency of ILCs.

ACT001 Inhibits Tumor Progression and Modulates Immune Responses in Non-Small Cell Lung Cancer.

Background: Advancements in non-small cell lung cancer (NSCLC) therapies have improved outcomes, but challenges like low immune response, drug resistance, and side effects persist. ACT001, a novel small-molecule inhibitor, shows promise in addressing these issues.

Methods: We evaluated ACT001's anti-tumor and immunomodulatory effects in NSCLC.

Effect of thawing methods on water mobility and microstructure of frozen pork.

The effect of seven different thawing methods (atmospheric temperature thawing (AT), microwave thawing (MT), microwave magnetic nanoparticles thawing (MMT), microwave vacuum thawing (MVT), far-infrared thawing (FT), far-infrared magnetic nanoparticles thawing (FMT) and far-infrared ultrasonic thawing (FUT)) on water holding capacity, water mobility and microstructure of frozen pork was examined. The results showed that the thawing loss (1.5 %), cooking loss (22.

Neuroglia and immune cells play different roles in neuroinflammation and neuroimmune response in post-stroke neural injury and repair.

Neuroinflammation and immune responses mediated by glial cells and immune cells play dual roles in the neural injury and repair of ischemic stroke (IS): glial cells and immune cells primarily have a detrimental role in the acute phase of IS, while they mainly serve a reparative function in the chronic phase. Thus, suppressing neuroinflammation and immune responses driven by glial and immune cells represents a major strategy in the treatment of IS. In this review, we provide an overview of the molecular mechanisms of neuroinflammation and immune responses mediated by glial cells and immune cells at different stages after IS and highlight the roles of different glial cells and immune cells in post-IS neural injury and repair.

Molecular characterization of Echinocandin resistance and the CHS3-mediated adaptive response in Candida glabrata bloodstream infections in Eastern China.

Background: Candidemia caused by Candida glabrata is a serious fungal infection, and rising echinocandin resistance presents a significant clinical challenge. Understanding the drug susceptibility profiles, molecular epidemiology, and mechanisms underlying adaptive echinocandin resistance in C. glabrata is crucial.

Elucidation of the Hemostatic and Anti-Inflammatory Effects of Walnut Shell Ethanol Extract by Network Pharmacology and Experimental Verification.

Effective hemostatic and anti-inflammatory measures are essential for wound healing. In this study, Ultra-performance liquid chromatography (UPLC) combined with network pharmacology was used to analyze the composition of walnut shell ethanol extract (WSEE), and in vivo and in vitro experiments elucidated its potential mechanisms of hemostatic and anti-inflammatory effects. The results showed that 45 components were identified from WSEE, and the NF-κB signaling pathway, IL-17 signaling pathway, etc.

Comparison of efficacy and safety of combined phacoemulsification, goniosynechialysis and goniotomy with trabeculectomy in advanced primary angle-closure glaucoma: a retrospective observational study.

Purpose: The study is a retrospective observational study. This study aimed to compare the intraocular pressure (IOP) lowering effect of phacoemulsification combined with intraocular lens implantation (PEI), Goniosynechialysis (GSL), and goniotomy (GT) vs. trabeculectomy (TRAB) in eyes with medically uncontrolled advanced primary angle-closure glaucoma (PACG) at a 12-month follow-up.

Ion Valency as a Molecular Switch for Salt-Resistant Underwater Adhesion.

Achieving underwater adhesion remains challenging due to the disruption of interfacial interactions by hydration layers and the ionic environment. This study shows how high adhesion in a saline environment can be achieved in adhesive peptide systems relying on π-π and cation-π interactions using multivalent ions. Monovalent ions (K) disrupt native peptide-peptide interactions, drastically reducing adhesion strength.

Network analysis of emotional symptoms and cognitive function changes in primary insomnia.

Background: Primary insomnia is a common and complex disorder characterized by a range of symptoms and consequences of varying severity. Long-term sleep disturbances can lead to anxiety, depression and cognitive dysfunctions. This study aimed to investigate the relationship between emotional changes and cognitive dysfunction in patients with primary insomnia and to explore the factors influencing the disorder.

C─C Bond Formation via Reductive Elimination at Rare Earth Centers.

Reductive elimination is one of the most important elementary reactions employed for constructing various chemical bonds in transition metal complex-mediated transformations. However, due to the lack of two-electron transfer, rare earth (RE) centers have been considered incapable of promoting reductive elimination, thus limiting the reactivity of RE complexes and their applications in synthetic chemistry. In the presented study, we demonstrated the reactions of β-diketiminate-supported dialkyl RE(III) complexes (L-κN,N')RE(III)(CHSiMe)(THF) (1-RE, RE = Y, Er, Yb, and Lu; L = {[(2,4,6-MeCH)NCMe]CH}) with azobenzene at ambient conditions, resulting in the formations of (L-κN,N')RE(III)(η-cis-PhNNPh)(THF) (2-RE) and 2-[(trimethylsilyl)methyl]azobenzene (I) via a reductive elimination-powered pathway.

A Highly Sensitive Low-Temperature N-Butanol Gas Sensor Based on a Co-Doped MOF-ZnO Nanomaterial Under UV Excitation.

Volatile organic compounds (VOCs) are presently posing a rather considerable threat to both human health and environmental sustainability. Among these, n-butanol is commonly identified as bringing potential hazards to environmental integrity and individual health. This study presents the creation of a highly sensitive n-butanol gas sensor utilizing cobalt-doped zinc oxide (ZnO) derived from a metal-organic framework (MOF).

A Comprehensive Review of the Role of the Microbiota-Gut-Brain Axis via Neuroinflammation: Advances and Therapeutic Implications for Ischemic Stroke.

The human gastrointestinal tract harbors a complex and diverse microbial community. Emerging evidence has revealed bidirectional communication between the gut microbiome and the central nervous system, termed the "microbiota-gut-brain axis". This axis serves as a critical regulator of glial cell function, positioning it as an essential target for ameliorating the onset and progression of ischemic stroke.

Copper Pyrithione Induces Hepatopancreatic Apoptosis and Metabolic Disruption in : Integrated Transcriptomic, Metabolomic, and Histopathological Analysis.

Copper pyrithione (CuPT), an emerging biocide used in ship antifouling coatings, may accumulate in marine sediments and pose risks to non-target organisms. However, current research on CuPT toxicity remains limited. , one of the world's most important aquaculture shrimp species, relies heavily on its hepatopancreas for energy metabolism, detoxification, and immune responses.