Therapeutic effects of the n-butanol extract of Potentilla freyniana Bornm. in hepatocellular carcinoma cells.

Objective: This study aimed to integrate network pharmacology, bioinformatics analysis, molecular docking, and experimental validation to construct a "component-target-pathway" multidimensional network model, systematically elucidate the potential mechanisms underlying the therapeutic effects of the extract of Potentilla freyniana Bornm. (PFB) on hepatocellular carcinoma (HCC), and thereby clarify its pharmacological basis.

Methods: HCC datasets were retrieved from GEO and TCGA databases, and the DEGs were screened.

Succinic Acid-Induced Macrophage Endocytosis Promotes Extracellular Vesicle-Based Integrin Beta1 Transfer Accelerating Fibroblast Activation and Sepsis-Associated Pulmonary Fibrosis.

Sepsis-associated pulmonary fibrosis (SAPF) is a life-threatening condition driven by persistent fibroblast activation and excessive extracellular matrix (ECM) deposition. While metabolic reprogramming, profibrotic extracellular vesicles (EVs), and integrin activation are implicated in pulmonary fibrosis, their interplay remains unclear. This study reveals that succinic acid, a product of glycometabolic reprogramming, promotes macrophage-mediated endocytosis, driving the release of profibrotic EVs.

Fluid shear stress activates a targetable mechano-metastatic cascade to promote medulloblastoma metastasis.

Biofluid flow generates fluid shear stress (FSS), a mechanical force widely present in the tissue microenvironment. How brain tumour growth alters the conduit of biofluid and impacts FSS-regulated cancer progression is unknown. Dissemination of medulloblastoma (MB) cells into the cerebrospinal fluid initiates metastasis within the central nervous system.

Global trends of antimicrobial resistance and virulence of Klebsiella pneumoniae from different host sources.

Background: Klebsiella pneumoniae is ubiquitous in animals, humans, and the environment, facilitating the dissemination of antimicrobial resistance (AMR) and virulence traits. Most studies are primarily focused on human clinical isolates, leaving critical gaps in understanding non-human reservoirs and cross-species transmission risks.

Methods: We combined large-scale genomic analyses with in vitro and in vivo infection models to characterize the evolutionary dynamics of 2809 K.

The efficacy and safety of pharmacotherapy for girls with central precocious puberty or early normal puberty: a retrospective cohort study.

The Chinese Expert Consensus on central precocious puberty (CPP) defines girls' rapid sexual development before age 8 as CPP; while after age 8 as early normal puberty (ENP). And the use of recombinant human growth hormone (rhGH) for CPP and ENP is off-label and lacks reliable evidence for clinical practice. This study only included girls due to the low prevalence among boys.

Efficacy of manipulation and therapeutic exercise applied to the neck region of patients with temporomandibular disorders: A systematic review and meta-analysis.

Objective: This study aimed to evaluate comprehensively and quantitatively the efficacy of manipulation and therapeutic exercise acting on the neck region of patients with myogenous temporomandibular joint disorders (TMD) using meta-analysis.

Methods: Randomized controlled trials (RCTs) in regard to this issue were searched in electronic databases. The timeframe was from the starting year of inclusion in each database to 25 December 2024 for all searches.

Improving peroxidase-like activity of porphyrin-based metal-organic framework by metal ratio tuning for detection and degradation of aflatoxin B in dried fruits.

Herein, the tetra (4-carboxyphenyl) porphyrin (TCPP) based metal-organic frameworks with high peroxidase (POD)-like activity were designed and fabricated for detection and degradation of aflatoxin B (AFB) in dried fruits. The strategy of metal ratio (Fe/Co/Ni) tuning was adopted to obtain the optimal enzymatic activity of TCPP-Fe/Co/Ni. The aptamer-modified TCPP-Fe/Co/Ni/ssDNA composite specifically binds AFB, with the presence of AFB inhibiting the POD-like activity of the material, thus establishing a rapid and sensitive colorimetric method for AFB detection.

Identification of DPP-IV Inhibitory Peptides from Eggshell Membranes: Structure-Activity Relationships, Digestive Stability, and Molecular Mechanisms.

Eggshell membrane (ESM), a protein-rich byproduct of the egg processing industry, contains latent bioactive sequences with physiological relevance, but remains largely underutilized. To evaluate its potential as a novel source of dipeptidyl peptidase-IV (DPP-IV) inhibitory peptides, ESM was enzymatically hydrolyzed using five proteases, among which alkaline proteases exhibited the most effective hydrolysis. The resulting hydrolysates were systematically assessed for DPP-IV inhibitory activity, amino acid composition, and peptide sequence characteristics.

Lung metastasis and recurrence is mitigated by CAR macrophages, in-situ-generated from mRNA delivered by small extracellular vesicles.

Cancer metastasis and recurrence remain the leading causes of cancer-related mortality, and lung is a major metastatic anatomical location. Chimeric antigen receptor macrophages (CAR-M) represent promising candidates for cancer therapy owing to their superior tumour-infiltrating and antigen-specific phagocytotic abilities, and to being professional antigen presenting cells. However, broader applications of CAR-Ms face challenges such as complex manufacturing processes and predominant accumulation in the liver following intravenous administration.

Luteolin attenuates type 2 inflammation in asthmatic mice induced by OVA by regulating IL-33/ST2- GSK3β-M2 macrophage polarization.

Allergic asthma is a prevalent non-infectious inflammatory disease characterized by type 2 inflammation. Although multiple treatment options are available, their efficacy is often limited due to the heterogeneous nature of asthma. Luteolin (LUT), a naturally occurring flavonoid, has demonstrated therapeutic potential in various inflammatory conditions.

NCOA4-Mediated Ferritinophagy Induces Ferroptosis and Enriches Ferritin-Containing EVs via Ferritin Phase Separation to Promote Mechanical Ventilation-Induced Pulmonary Fibrosis.

Introduction: Mechanical ventilation (MV) is essential for treating respiratory failure but can paradoxically lead to pulmonary fibrosis. The mechanisms of MV-induced pulmonary fibrosis (MVPF) remain poorly understood. Ferritinophagy, a novel autophagic process, regulates ferroptosis and the release of ferritin-containing extracellular vesicles (EVs), both of which may contribute to MVPF.

Identification of a novel mild isolate of areca palm necrotic spindle-spot virus (ANSSVm) lacking two cysteine proteases (HC-Pro1 and HC-Pro2).

Areca palm necrotic spindle-spot virus (ANSSV) encodes two tandem cysteine proteases, HC-Pro1 and HC-Pro2, at its N-terminal region, essential for maintaining viral infectivity. Deletion of either protease from an infectious clone abolishes infectivity. We report a mild ANSSV isolate, designated ANSSVm, from areca palm leaves exhibiting mild chlorosis.

ARTREM2 macrophage induced pathogenic immunosuppression promotes prostate cancer progression.

The androgen receptor (AR) usually drives prostate cancer cell growth, yet its role in immune cells such as tumour-associated macrophages (TAMs), remains unclear. We find that macrophages co-expressing AR and triggering receptor expressed on myeloid cells-2 (TREM2) exhibiting potent immunosuppressive and tumour-promoting effects. Genetic ablation of TREM2 combined with pharmacological blockade of AR, significantly reduces tumour progression in prostate cancer mouse models.

TSPEAR S475TfsX79 mutation does not affect auditory function, tooth morphology or hair development in mice.

TSPEAR is a member of the EAR (epilepsy-associated repeat) protein family with poorly characterized function. Several lines of evidence suggest that mutations in the human TSPEAR gene are associated with hearing loss or ectodermal dysplasia. Although tooth abnormalities and a reduced capacity for caudal fin regeneration were observed in the Tspeara;Tspearb knockout zebrafish model, there have been no reports of the Tspear knockout mouse model to date, which hampers further investigation of its physiological role.

ATGL modulates lipid-protein interactions to restore foaming capacity in yolk-residue egg white systems.

Residual egg yolk in egg white, a common issue in industrial processing, impairs foaming properties vital for aerated bakery products. This study reveals that yolk residue ≥0.7 % (w/w) destabilizes foams via lipid bridge formation and interfacial protein network disruption.

Platelet membrane-coated nanoparticles inhibit platelet activation and neutrophil extracellular traps formation in acute lung injury.

Background: Platelets play a critical role in the pathophysiology of acute lung injury (ALI) by activating neutrophils and promoting the formation of neutrophil extracellular traps (NETs). Excessive NETs formation exacerbates lung injury by triggering inflammation, impairing essential alveolar macrophage functions and activating the coagulation cascade. Consequently, inhibiting NETs formation represents a promising strategy for treating ALI.

Mechanisms of nurses' AI use intention formation in Sichuan, Yunnan, and Beijing, China: mediating effects of AI literacy via self-efficacy-to-attitude pathways.

Aim: This study aimed to explore the formation mechanism of artificial intelligence (AI) usage intention among nurses in public hospitals in Beijing, Sichuan, and Yunnan, China, analyzing the influence of AI literacy on usage intention through AI self-efficacy and general attitudes.

Methods: A multi-center cross-sectional design was adopted, surveying 901 registered nurses via the Wenjuanxing platform from December 26, 2024, to February 25, 2025, with 878 valid questionnaires returned (effective rate 97.45%).

Oxidative stress-responsive fluorescent polymer nanoplatform regulate lipid metabolism through lipophagy.

Lipid droplets are dynamic organelles involved in lipid metabolism and various metabolic disorders. Herein, we report the design and synthesis of a novel amphiphilic polymer based on 18β-glycyrrhetinic acid (GA). GA was chemically conjugated to polyethylene glycol (PEG) and further functionalized with a lipid droplet-targeting fluorescent probe D via an oxalate ester bond, then self-assembly yielding PGOD nanoplatform.

The Key Egg White Proteins Involved in Foam Formation and Foam Stability.

This study determined the major egg white proteins for foaming and foam stability. The natural and lysozyme-free egg whites were 2x diluted and blended with a KitchenAid blender for 5 minutes to make foams. The foams were collected, held at room temperature for 2 hr, and drained.

The Role of Estrogen in Mitochondrial Disease.

This review aims to investigate the potential role of estrogen in various mitochondrial diseases, such as Leber's Hereditary Optic Neuropathy and Mitochondrial Encephalomyopathy, Lactic Acidosis, and Stroke-like Episodes, focusing on its effects on aging, oxidative stress, mitochondrial biogenesis, and mitophagy. Mitochondrial diseases have become important in modern medical research due to their complex genetic background and diverse clinical manifestations. Studies in recent years have shown that estrogen plays an essential role in physiological regulation and may also affect the health status of cells by regulating mitochondrial function, which in turn affects the occurrence and development of diseases.

A quadruplex real-time fluorescent quantitative PCR detection method for identification and serotyping of Pasteurella multocida.

Pasteurella multocida is a zoonotic pathogen with the ability to infect a diverse range of hosts, including humans through contact with pets. Due to its diverse capsular serogroups, rapid and accurate identification, as well as serotyping, are essential for the effective prevention and control of infections caused by this bacterium. This study developed a quadruplex quantitative PCR (qPCR) method for the identification and serotyping of P.

Cantharidin-Loaded Nanomedicine Modified with Amphiphilic Peptides Induce Immunogenic Cell Death to Enhance PD-1 Blockade.

Recent advances in immune checkpoint inhibitors (ICIs), particularly those targeting PD-1/PD-L1, have transformed cancer treatment. However, their efficacy is often hampered by the immunosuppressive tumor microenvironment (TME). Here, we developed a cantharidin-loaded nanomedicine (CA@TPGS-AmP), composed of D-α-tocopheryl polyethylene glycol succinate (TPGS) and naturally derived amphiphilic peptides (AmP), designed to enhance PD-1 blockade via induction of immunogenic cell death (ICD).

Global pattern, trend, and cross-country health inequality of adult acne aged 25 + years from 1990 to 2021, a comprehensive analysis for global burden of disease and global dietary database.

Background: The epidemiology and burden of adult acne have not been comprehensively evaluated. This study aims to analyze the global disease burden of adult acne from 1990 to 2021 and explore potential risk factors.

Methods: With Global Burden of Disease (GBD), we got all the incidence, prevalence, disability-adjusted life years (DALYs), and corresponding age-standardised rate (ASR) of acne vulgaris (25 + years).

Therapeutic radiation drives leptomeningeal dissemination of medulloblastoma through an innate immune process.

Leptomeningeal metastases are the most important source of morbidity and mortality for medulloblastoma patients. Radiation of the entire brain is highly effective in the treatment and/or prevention of medulloblastoma leptomeningeal metastases. Infants treated on clinical trials with focal tumor radiation recur metastatically, whereas infants treated with only chemotherapy relapse locally.

Immobilization of trypsin on resin-carriers: A novel strategy to realize controlled hydrolysis and enhance the foaming properties of pasteurized and frozen-thawed egg white.

Through mechanistic analyses of protein structure and interfacial properties, this study revealed that both pasteurization and freeze-thaw treatments decreased the foaming capacity (FC) of egg white to 265 % and 95 %, respectively, by inducing protein aggregation due to thermal or ice crystal damage. To address this, trypsin and papain hydrolysis were employed to depolymerize pasteurized egg white (PEW) and five freeze-thaw cycles treated egg white (FT-5). The maximum FC (403 %) achieved with a 60-min trypsin treatment.