Updated reference intervals for serum cystatin C and creatinine based on age and sex variations.

Unlabelled: We investigated inconsistencies between results for cystatin C (Cys C) and creatinine (Crea) in patients undergoing health check-ups. Data from 36,836 individuals who underwent health checkups at Huashan Hospital over the previous 3 years were analyzed. We extracted laboratory results of 10 biomarkers and information on sex and age.

The integration of psychological education and moral dilemmas from a value perspective.

The rapid evolution of internet technologies has emphasized the importance of integrating psychological education with moral dilemmas across diverse sectors. This paper investigates the interrelationship between psychological education and moral reasoning, proposing that this integration represents a pivotal approach for fostering effective educational strategies. Leveraging deep learning models, we aim to enhance both the scientific rigor and theoretical understanding of how these two domains intersect.

Exploring the Time to Onset and Early Predictors of Poststroke Spasticity Combined With Surface Electromyography: Protocol for a Nested Case-Control Study.

Background: Poststroke spasticity (PSS) is a frequent sequela in patients who have experienced stroke. This form of paralysis is more prevalent compared to other poststroke sequelae and is among the most challenging and complex symptoms to manage. Surface electromyography (sEMG) can reflect the physiological information of muscles in real time and is highly beneficial in diagnosing neuromuscular diseases in clinical medicine.

KFLC-index distinguishes multiple sclerosis from anti-myelin oligodendrocyte glycoprotein and aquaporin 4 diseases in a Chinese cohort.

Background: Kappa free light chain (KFLC) index has emerged as a diagnostic biomarker for multiple sclerosis (MS). This study aims to evaluate the diagnostic accuracy of the KFLC-index in Chinese patients with MS, and its capacity to discriminate MS from myelin oligodendrocyte glycoprotein antibody-associated disease (MOGAD) and neuromyelitis optica spectrum disorders with aquaporin-4 antibody (AQP4+NMOSD).

Methods: 428 patients tested for KFLC-index were enrolled in the study, including 130 patients with MS, 41 with MOGAD, 25 with AQP4+NMOSD, 123 with other inflammatory or infectious neurological disorders (OIND) and 109 with non-inflammatory neurological disorders (NIND).

A quadruplex real-time fluorescent quantitative PCR detection method for identification and serotyping of Pasteurella multocida.

Pasteurella multocida is a zoonotic pathogen with the ability to infect a diverse range of hosts, including humans through contact with pets. Due to its diverse capsular serogroups, rapid and accurate identification, as well as serotyping, are essential for the effective prevention and control of infections caused by this bacterium. This study developed a quadruplex quantitative PCR (qPCR) method for the identification and serotyping of P.

Isolation, Passage, and Pathogenicity of a Newly Isolated Strain From Hubei, China.

is the causative agent of porcine proliferative enteropathy (PPE)-a disease of great economic impact to the global pig industry, but the isolation and continuous passage culture of this bacterial species is very difficult which limits the development of inactivated or live vaccines. While China is the largest pig rearing country in the world, only one study has reported the isolation of . .

Genomic characteristics and pathogenicity of three newly isolated NADC34-like PRRSV strains in China and evaluation of one strain for inactivated vaccine candidate.

Porcine reproductive and respiratory syndrome (PRRS) has caused substantial economic losses to the pig industry. Recently, the emergence of NADC34-like PRRS virus (PRRSV) has made the worldwide circulation of PRRSV more complex. In 2017, the first detection of NADC34-like PRRSV in China was reported, but the pathogenicity and biological characteristics of this novel PRRSV variant remain to be further elucidated.

Task Similarity-Aware Cooperative Computation Offloading and Resource Allocation for Reusable Tasks in Dense MEC Systems.

As an emerging paradigm for supporting computation-intensive and latency-sensitive services, mobile edge computing (MEC) faces significant challenges in terms of efficient resource utilization and intelligent task coordination among heterogeneous user equipment (UE), especially in dense MEC scenarios with severe interference. Generally, task similarity and cooperation opportunities among UE are usually ignored in existing studies when dealing with reusable tasks. In this paper, we investigate the problem of cooperative computation offloading and resource allocation for reusable tasks, with a focus on minimizing the energy consumption of UE while ensuring delay limits.

Development and application of a triplex real-time PCR method for the detection of , , and .

(LI), (Bhy), and (Cp) are three common swine enteric pathogens widespread worldwide, leading to huge economic losses to the pig industry. LI, Bhy, and Cp are co-infected and should not be distinguished, and the detection of bacterial nucleic acids in feces allows rapid identification of whether a herd is infected with these three pathogens. In this study, we developed and optimized a TaqMan triple quantitative real-time PCR (qPCR) method for LI, Bhy, and Cp, which was validated and applied to the analysis of actual samples from pigs.

Liquid chromatography-tandem mass spectrometry assay for simultaneous quantification of catecholamines and metabolites in human plasma and cerebrospinal fluid.

Catecholamines (CAs) and their metabolites in human cerebrospinal fluid (CSF) and plasma are potential biomarkers of Alzheimer's disease (AD) and facilitate early diagnosis. Liquid chromatography-tandem mass spectrometry is the gold standard method for analyzing CAs. The objective of this study was to develop and validate a liquid chromatography-tandem mass spectrometry assay capable of simultaneously quantifying dopamine (DA), epinephrine (E), norepinephrine (NE), metanephrine (MN), normetanephrine (NMN), and 3-methoxytyramine (3-MT) in both human CSF and plasma.

Integrated Analysis of Transcriptomics and Proteomics Provides Insights into the Accumulation Mechanism of Ascorbic Acid in Tratt.

Tratt (RRT) is widely cultivated in Guizhou Province, China. In recent years, RRT has emerged as one of the most promising new fruit crops in China, primarily because of its remarkably high levels of ascorbic acid (AsA). In this research, we assessed the AsA levels in RRT across various growth phases.

Leguminous proteins as beneficial baking emulsifiers: A comparative study with traditional sucrose ester.

In recent years, pursuing healthier and more sustainable food ingredients has increased interest in plant-based alternatives to traditional synthetic emulsifiers. In this study, the properties of five legume proteins: soybean protein isolate (SPI), pea protein isolate (PPI), black bean protein isolate (BBPI), white Canavalia protein isolate (WCAI), and white kidney bean protein isolate (WKBPI) were compared with that of the conventional emulsifier sucrose ester (SE), and add them as emulsifiers to the cake making process. The centrifugal instability index of SPI stabilized emulsion (0.

Discovery of viruses and bacteria associated with swine respiratory disease on farms at a nationwide scale in China using metatranscriptomic and metagenomic sequencing.

Respiratory disease (RD) is a worldwide leading threat to the pig industry, but there is still limited understanding of the pathogens associated with swine RD. In this study, we conducted a nationwide genomic surveillance on identifying viruses, bacteria, and antimicrobial resistance genes (ARGs) from the lungs of pigs with RD in China. By performing metatranscriptomic sequencing combined with metagenomic sequencing, we identified 21 viral species belonging to 12 viral families.

Roles of gut microbiome-associated metabolites in pulmonary fibrosis by integrated analysis.

Lung diseases often coincide with imbalances in gut microbiota, but the role of gut microbiota in pulmonary fibrosis (PF) remains unclear. This study investigates the impact of gut microbiota and their metabolites on PF. Serum and lung tissues of normal, bleomycin (BLM)- and silica-induced mice showed significant differences in gut microbiota.

Mathematical Expression and Prediction of VOCs Adsorption Capacity and Isotherm.

Adsorption capacity prediction, which needs to be based on the precise structure-capacity relationship, is important for better adsorbent design. However, the precise adsorption contribution coefficients of pores of different sizes for volatile organic compound (VOC) adsorption remain unclear. Herein, a control variable method is employed as a generative model to realize the numerization of the precise structure-capacity relationship.

Ring-Enhancement on CEUS: Is it Useful in the Differential Diagnosis of Solid Thyroid Nodules?

To investigate the efficiency of contrast-enhanced ultrasound (CEUS) features, particularly ring-enhancement patterns, in the differential diagnosis of thyroid nodules. 302 nodules with CEUS ring-enhancement were retrospectively enrolled, including 135 benign and 167 malignant ones. The ring-enhancement patterns were classified into regular and irregular hyper- or hypo-ring enhancement.

Metarhizium spp. encode an ochratoxin cluster and a high efficiency ochratoxin-degrading amidohydrolase revealed by genomic analysis.

Introduction: Ochratoxins (OTs) are worldwide regulated mycotoxins contaminating a variety of food-environment and agro-environment. Several Aspergillus and Pencillium species synthesize OTs from a six-gene biosynthetic gene cluster (BGC) to produce the highly toxic final product OTA. Although many studies on OTA-degrading enzymes were performed, high efficiency enzymes with strong stability are extremely needed, and the OTA degrading mechanism is poorly understood.

What Chinese Women Seek in Mental Health Apps: Insights from Analyzing User Posts during the COVID-19 Pandemic.

Gender disparity poses a prominent obstacle to achieving effective mental health outcomes in digital healthcare. Despite women being more inclined to use mental health apps and seeking designs tailored to their specific needs, there is limited research on the factors influencing female users' engagement with these apps. The COVID-19 pandemic has further exacerbated its disproportionate impact on women's mental health.

Needle-scalpel therapy inhibits the apoptosis of nucleus pulposus cells via the SDF-1/CXCR4 axis in a rat degenerative cervical intervertebral disc model.

As a common disease, cervical spondylosis (CS) results from the degeneration of the cervical intervertebral disc. However, there are still no effective clinical strategies for the treatment of this disease. Needle-scalpel (Ns), a therapy guided by traditional Chinese medicine theory, alleviates intervertebral disc degradation and is widely used in the clinic to treat CS.

Establishment and application of a quadruplex real-time RT-qPCR assay for differentiation of TGEV, PEDV, PDCoV, and PoRVA.

Porcine viral diarrhea is a common ailment in clinical settings, causing significant economic losses to the swine industry. Notable culprits behind porcine viral diarrhea encompass transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and porcine rotavirus-A (PoRVA). Co-infections involving the viruses are a common occurrence in clinical settings, thereby amplifying the complexities associated with differential diagnosis.

Fecal PCR survey and genome analysis of in China.

Proliferative enteropathy caused by is an important economic associated disease to pig industry, but the knowledge about the prevalence of in pig farms in China is limited. In addition, there is no complete genome sequence available for isolates from China. In this study, we developed a TaqMan qPCR for the screening of by targeting the bacterial 16S rDNA gene.

Treatment response assessment to chemotherapy with bevacizumab for colorectal liver metastasis by contrast-enhanced ultrasound.

Background: Though contrast-enhanced ultrasound (CEUS) perfusion parameters have been approved to be potential indicators for response to chemotherapy in solid tumors, their ability in assessment of colorectal liver metastasis (CRLM) to chemotherapy with bevacizumab (Bev) has rarely been investigated.

Methods: From March 2021 to May 2022, 115 consecutive CRLM patients with CEUS pre- and post-2 months' chemotherapy with Bev were prospectively enrolled. One target lesion per patient underwent CEUS quantitative analysis with SonoLiver software.

A Metamodel-Based Multi-Scale Reliability Analysis of FRP Truss Structures under Hybrid Uncertainties.

This study introduces a Radial Basis Function-Genetic Algorithm-Back Propagation-Importance Sampling (RBF-GA-BP-IS) algorithm for the multi-scale reliability analysis of Fiber-Reinforced Polymer (FRP) composite structures. The proposed method integrates the computationally powerful RBF neural network with GA, BP neural network and IS to efficiently calculate inner and outer optimization problems for reliability analysis with hybrid random and interval uncertainties. The investigation profoundly delves into incorporating both random and interval parameters in the reliability appraisal of FRP constructs, ensuring fluctuating parameters within designated boundaries are meticulously accounted for, thus augmenting analytic exactness.