NIR-driven dual enzyme-like activity of CuS@CeO modified thermally enhanced hydrogel for antibacterial therapy and wound healing.

In bacterial wound healing, continuous controlled oxygen delivery to injured tissue, coupled with bacterial elimination and inflammation reduction, is crucial for promoting tissue regeneration and repair. To address this, we designed a multimodal synergistic treatment strategy based on nanozymes to enable oxygen-controlled release while achieving antibacterial and anti-inflammatory effects. We developed a polydopamine-hyaluronic acid (PDA-HA) hydrogel composite system incorporating CuS NPs and CeO₂ nanozymes.

Lignin-derived self-assembled 2D N-doped carbon nanosheets as effective oxygen-redox electrocatalysts for rechargeable zinc-air batteries.

Two-dimensional (2D) carbon nanomaterials are widely used in electrocatalytic reactions due to their enhanced electron mobility and excellent structural stability. Nevertheless, the complexity of its synthesis process limits its large-scale application. In this study, layered porous 2D lignin-derived carbon nanosheets were successfully synthesized using various salts as templates combined with an antisolvent technique.

Optimization of Production Conditions for Extracellular Vesicles from Lacticaseibacillus Paracasei LH23 Using Response Surface Methodology: Enhancement of Yield and Exploration of Stability, Safety, and Anti-inflammatory Capacity.

Extracellular vesicles (EVs) from probiotics containing multiple bioactive ingredients play important roles in intercellular communication, immunomodulation, and tumor suppression, therefore possessing enormous potential for improving health, targeted drug delivery, and vaccine development. However, the difficulty in extraction and low yield limits the widespread application of EVs. Here, response surface methodology was employed to optimize the extraction conditions of Lacticaseibacillus paracasei LH23 EVs (LP-Evs).

Interaction Mechanism of Casein with Ferritin and Iron Ion: The Structure, Iron Oxidation/Release, and Functionality Exploration.

Ferritin is a major iron storage protein, which can regulate the dynamic balance of iron in organisms. Casein, the major protein in milk, has a unique phosphorylated structure that makes it a natural and versatile metal chelator. This study investigated the interaction mechanism of casein with shrimp ferritin and iron ions and evaluated the structure, iron oxidation/release, and functionality of ferritin.

High-Vigor Rootstock Exacerbates Herbaceous Notes in L. cv. Cabernet Sauvignon Berries and Wines Under Humid Climates.

Rootstocks are widely used in viticulture as an agronomic measure to cope with biotic and abiotic stresses. In winegrapes, the aroma is one of the major factors defining the quality of grape berries and wines. In the present work, the grape aroma and wine aroma of Cabernet Sauvignon (CS) grafted on three rootstocks were investigated to inform the selection of rootstocks to utilize.

Probiotics, Prebiotics, Synbiotics, Postbiotics, and Paraprobiotics-New Perspectives on Functional Foods and Nutraceuticals.

Probiotics and their derivatives (including prebiotics, synbiotics, postbiotics, and paraprobiotics) have been extensively proven to regulate the gut microbiota balance and promote host health [...

Study on the Electro-Optical Properties of Polymer-Dispersed Liquid Crystals Doped with Cellulose Nanocrystals.

The present study focuses on the effect of doping KH560-modified cellulose nanocrystals (CNCs) on the electro-optical characteristics of polymer-dispersed liquid crystals (PDLCs). PDLC films were fabricated through the polymerization-initiated phase separation (PIPS) process and doped with CNC nanoparticles at various concentrations. At low concentrations, the CNCs at the interface, by virtue of their unique chiral characteristics, induce an orderly arrangement of liquid crystal molecules.

Enzymatic deamidation soy protein isolate stabilized high internal phase emulsion as a new fat substitute in meat patties.

Plant-based proteins are being explored as structuring agents to mimic the texture of animal fat in meat alternatives, contributing to the development of lipid-like structural matrices in plant-based meat products. Plant-based proteins are emerging as alternatives to animal fats, aligning with the food industry's low-fat goals. However, issues such as protein aggregation and sedimentation limit their application as fat substitutes.

Machine Learning-Enhanced Nanozyme Sensor Array for Accurate Multiple Quinolone Antibiotics Recognition.

The overuse of quinolone antibiotics (QNs) seriously endangers human health and the ecological environment. In this work, a copper dihydroxosulfate (Cu(OH)SO) nanosheet exhibiting notable peroxidase-like (POD) and laccase-like (LAC) activities has been developed in basic deep eutectic solvents (DES). The unique physicochemical properties of QNs allow them to enhance the POD activity of Cu(OH)SO, and with the extension of reaction time, this enhancement gradually intensifies.

Preparation of water-soluble flame retardant by cottonseed meal degradation for durable homologous flame retardant of cotton fiber.

The concept of homologous flame retardant of cottonseed meal to cotton fiber is reported. According to the chemical bond characteristics of the amide bond, ester bond, and glycosidic bond in crude protein, crude fiber, and crude fat of cottonseed meal, we developed a new system of HO-HPO degradation of dissolved cottonseed meal to prepare flame retardant. The study focuses on preparing water-soluble flame retardant (HO-HPO degradation Cottonseed meal, HPCm) by one-pot degradation of cottonseed meal and achieving durable flame retardancy for cotton fiber.

An inhalable composite particulate system for targeted delivery of therapeutics deep into small airways: in vitro and in vivo evaluation.

Chronic obstructive pulmonary disease (COPD) is characterized by the airflow limitation due to chronic inflammation and excessive airway mucus secretion. Targeted delivery of therapeutics deep into small airways is the key step in treatment of COPD. In this study, we designed an inhalable composite particulate system with nano in micro structure for targeted delivery of therapeutics deep into small airways.

Utilizing the SacB-mediated gene editing system in Komagataeibacter xylinus to explore the function of bacterial cellulose synthase.

Bacterial cellulose (BC) is a microbial polysaccharide, which is widely used in biotechnology, food, medicine, and other industries. Although existing genetic toolkits have laid a solid foundation for the genetic manipulation of BC-producing strains, there is still room for improvement in enhancing editing efficiency, simplifying operational procedures, and achieving scarless modifications. In the present study, we developed a SacB-based system, pK18mobsacB, to achieve marker-free gene editing with an efficiency of up to 83.

Molecular Networking-Driven Discovery of an Unprecedented Cyclotetrapeptide and Potent Anti-MDR -Glycoprotein Modulating Piperazines from .

Multidrug resistance (MDR) remains a significant challenge in cancer chemotherapy. Seeking novel MDR modulators, we employed a molecular networking (MN)-guided strategy to explore the endophytic fungus . This led to the targeted isolation of an unprecedented cyclotetrapeptide, templicolamide A (, featuring a rare β-enamino acid), and four piperazine derivatives (-), including the new helvamide E () with a unique bicyclic scaffold.

Unspecific peroxygenases and P450-derived peroxygenases as green biocatalysts driven by HO for sustainable applications: A review.

Unspecific peroxygenases (UPOs) are promising biocatalysts capable of catalysing selective oxygenation of organic substrates using hydrogen peroxide (HO) as the sole oxidant under mild conditions. Sharing a broad substrate range with cytochrome P450 monooxygenases, UPOs function without costly cofactors or auxiliary proteins, making them attractive for industrial biocatalysis. However, limited heterologous expression and poor HO tolerance restrict their broader application.

Temporal and spatial variation of pCO in the coastal waters of the Pearl river estuary in winter and summer and its influencing factors: A model study.

Clarifying the complex physical-chemical-biological interactions is essential for evaluating the partial pressure of carbon dioxide (pCO) in coastal waters. In this paper, a high-resolution three-dimensional coupled physical-biogeochemical model was established for the northern South China Sea (NSCS). The spatiotemporal distribution of the pCO in the coastal waters of the Pearl River estuary (CWPRE) was simulated, and its controlling factors were analyzed.

Improving Phase Stability of α-CsPbI through Combined Strain-Doping Engineering: Insights from First-Principles Calculations and Machine Learning.

The all-inorganic perovskite of cubic CsPbI (α-CsPbI) exhibited high thermal stability, owing to the absence of volatile organic cations. However, the poor phase stability at room temperature limits its practical applications. In this work, the phase transition mechanism of CsPbI from the cubic phase (α) to the tetragonal phase (β) was studied by first-principles calculations.

Assessment of Soil Organic and Inorganic Carbon Stocks in Coastal Salt Marshes of China: Key Mechanisms Elucidated.

Coastal salt marsh ecosystems are among the most important blue carbon (C) sinks. However, due to disparate data sources, limited sample sizes, and inconsistent methodologies, large variations exist in blue C stock estimation. Furthermore, studies focus on soil organic carbon (SOC), with minimal attention given to soil inorganic carbon (SIC).

Co-fermentation of and enhances the flavor characteristic of mature coconut water: Insights from volatile and non-volatile profiles.

The mature coconut water (MCW) is rich in nutrients, but its sour and astringent taste makes it unsuitable for direct consumption. In this study, a co-fermentation system employing and was developed to enhance the flavor of MCW, wherein an indigenous 1-7-1 was screened for optimal sensory characteristics when co-fermented with A33. Co-fermentation changed odor and taste characteristics of MCW with a broad range of organic compounds and the high richness taste attribute.

Phosphatidylserine Ameliorates High-Fat Diet-Induced Obesity Through Modulating the Bile Acid Metabolism and Gut Microbiota.

Obesity has emerged as a prevalent clinical and public health concern in contemporary society, imposing significant economic and social burdens worldwide. Phosphatidylserine (PS), a functionally diverse phospholipid, demonstrates multiple biological activities. This study delves into the efficacy and underlying mechanisms of PS in mitigating high-fat diet (HFD)-induced obesity in mice.

Coordinated synthesis of aromatic amino acid derived pigments in yeast via global transcriptional engineering.

Background: Rational metabolic pathway engineering is capable of boosting upstream flux towards downstream synthesis of target products, such as aromatic amino acid derivatives. However, coordinated synthesis of multiple downstream derivatives faces difficulty of combinatorial optimization of cellular metabolism.

Results: We developed a strategy combining metabolic engineering optimization with the global transcriptional regulation of transcription factors (TFs) Spt15p and Gcn4p to optimize the synthesis of aromatic amino acid derivatives in yeast.

Investigating the role of pectin's amphiphilic group in ice recrystallisation inhibition.

This study investigated the ice recrystallisation inhibition (IRI) activity of pectin with different hydrophilic and hydrophobic groups through both experimental methods and molecular dynamic (MD) simulations. The experimental results revealed that pectin with a moderate degree of esterification (DE) of 51 % exhibited enhanced IRI activity, characterized by the smallest particle size (535.83 nm), polydispersity index of 0.

Diastereoselective synthesis of spiro[cyclopropane-1,3'-indolin]-2'-imines a sequential [1 + 2] annulation reaction of indolin-2-imines with α-aryl vinylsulfonium salts.

A sequential [1 + 2] annulation reaction of indolin-2-imines with α-aryl vinylsulfonium salts has been achieved for the first time, leading to a series of functionalized spiro[cyclopropane-1,3'-indolin]-2'-imines in 51-95% yields with high chemoselectivity and diastereoselectivity. The method is easy to manipulate, operates under mild conditions, and shows wide substrate scope with good functional group tolerance. Moreover, some of the selected spiro-annulated products exhibit promising anticancer activity against the A549 cell line, highlighting their potential for application in drug screening.

TUBB3/DTX LNPs Dry Powder Inhaler and an Efficient Delivery Device for Targeted Therapy of Lung Cancer.

Lung cancer is the most prevalent and deadly tumor globally, marked by drug resistance and a poor prognosis. Specialized formulations with strong lung targeting are currently lacking. Docetaxel (DTX) and TUBB3-siRNA represent chemotherapy and RNA interference (RNAi), two strategies for lung cancer treatment.

Salinity Gradients Override Hydraulic Connectivity in Shaping Bacterial Community Assembly and Network Stability at a Coastal Aquifer-Reservoir Interface.

The coastal zone presents complex hydrodynamic interactions among inland groundwater, reservoir water, and intruding seawater, with important implications for ecosystem functioning and water quality. However, the relative roles of hydraulic connectivity and seawater-driven salinity gradients in shaping microbial communities at the aquifer-reservoir interface remain unclear. Here, we integrated hydrochemical analyses with high-throughput 16S rRNA gene sequencing to investigate bacterial community composition, assembly processes, and co-occurrence network patterns across groundwater_in (entering the reservoir), groundwater_out (exiting the reservoir), and reservoir water in a coastal system.