Tissue-specific mRNA delivery and prime editing with peptide-ionizable lipid nanoparticles.

Lipid nanoparticles for mRNA delivery and gene editing have the potential to transform the current therapeutic landscape. Nonetheless, a major bottleneck using this technology is the difficulty in achieving cell- and tissue-specific delivery and avoiding liver accumulation. Here we report the rational design of peptide ionizable lipids to assemble lipid nanoparticles with organ-selective mRNA delivery.

Targeting the NLRP3 Inflammasome: Pharmacological Mechanisms for the Treatment of Dry Eye Disease.

Dry eye disease (DED) is a multifactorial disorder characterized by tear film instability due to reduced tear secretion, increased evaporation, or the production of poor-quality tears. This imbalance leads to ocular surface inflammation, tissue damage, and aberrant nociception, collectively imposing a substantial burden on patients' quality of life. Emerging evidence highlights the pivotal role of ocular surface inflammation in the pathophysiology of chronic DED.

Enhancing effects of glycine on methanesulfonic acid-driven new particle formation: Mechanistic insights and implications for atmospheric pollution.

New particle formation (NPF) influences the concentration of marine aerosols, and amino acids are important components of marine particulate matter. Glycine (Gly), the simplest and one of the most abundant amino acids in the atmosphere, has an unclear mechanism regarding its involvement in driving the nucleation of methanesulfonic acid (MSA). This study employs the density functional theory at DLPNO-CCSD(T)/aug-cc-pVTZ//ωB97X-D/6-31++G(d,p) level and the Atmospheric Cluster Dynamics Code (ACDC) to investigate the nucleation processes of the (Gly)(MSA) (m = 0-4 and n = 0-4) system.

Organic acid accumulation pattern and its key genes in Chinese cherry fruits.

Background: Chinese cherry [Cerasus pseudocerasus (Lindl.) G.Don], an economically important fruit species native to southwestern China, plays a key role in regional agriculture.

Thinned and Welded Silver Nanowires for Intelligent Pressure and Humidity Sensing Enabled by Machine Learning.

The rapid development of flexible and wearable technologies urgently requires high-performance and compatible flexible electrodes. Silver nanowires (AgNWs) are considered a promising conductive material, but their inherent structural drawbacks significantly hinder their widespread application. Here, an innovative and facile strategy is employed to simultaneously enhance the electrical and optical properties of AgNWs by precisely tuning their microstructure.

The chain mediating roles of spiritual well-being and hope between self-control and depressive symptoms among breast cancer patients: a cross-sectional study.

Background: Depressive symptoms were prevalent among breast cancer (BC) patients. This study aimed to examine the associations among self-control, spiritual well-being, hope, and depressive symptoms and to explore the chain mediating effect of spiritual well-being and hope in these associations among BC patients in China.

Methods:  A total of 458 BC patients (effective response rate: 91.

Sulforaphane attenuates aldose reductase-mediated platelet dysfunction in high glucose-stimulated human platelets via downregulation of the Src/ROS/p53 signaling pathway.

Background: Platelet abnormalities are well-recognized complications of type 2 diabetes mellitus (T2DM). High glucose (HG) increases platelet mitochondrial dysfunction, apoptosis and hyperreactivity in T2DM, which underlie the occurrence of thrombotic events. Sulforaphane (SFN) is a dietary isothiocyanate enriched in cruciferous vegetables and possesses multiple biological activities.

Enzymatic deamidation soy protein isolate stabilized high internal phase emulsion as a new fat substitute in meat patties.

Plant-based proteins are being explored as structuring agents to mimic the texture of animal fat in meat alternatives, contributing to the development of lipid-like structural matrices in plant-based meat products. Plant-based proteins are emerging as alternatives to animal fats, aligning with the food industry's low-fat goals. However, issues such as protein aggregation and sedimentation limit their application as fat substitutes.

The Full Genomic Sequence of the HLA-DQB1*06:97 Allele Identified Using PacBio Sequencing.

We report the full genomic sequence of the HLA-DQB1*06:97 allele.

Albumin-recruiting lipid nanoparticle potentiates the safety and efficacy of mRNA vaccines by avoiding liver accumulation.

The advent of mRNA vaccines represents a breakthrough in the realm of cancer therapy and the prevention of infectious disease. Nevertheless, traditional lipid nanoparticle (LNP)-based mRNA vaccines can accumulate in the liver post-intramuscular injection, posing a risk of hepatotoxicity and reducing efficacy. Here we develop an albumin-recruiting LNP system with high lymphatic drainage and no accumulation in hepatic tissue to potentiate the efficacy and safety of mRNA vaccines.

Assessing the risk of undetected cervical cancer in women with a biopsy of high-grade cervical intraepithelial neoplasia: predictive value of cytology and endocervical curettage (ECC).

Objective: To identify risk factors for a final diagnosis of cervical cancer in patients initially diagnosed with high-grade cervical intraepithelial neoplasia and to evaluate a combined Cytology-Endocervical Curettage (ECC) index for predicting cancer risk.

Methods: This retrospective study included 11,651 patients at West China Second University Hospital (WCSUH) with biopsy-confirmed high-grade cervical intraepithelial neoplasia over a five-year period. Patients were divided into two groups based on whether they ultimately received a diagnosis of cervical cancer following surgery.

Personalized 3D Vaccine Integrates Immunotherapy and Antiangiogenic Therapy to Prevent Oral Squamous Cell Carcinoma Recurrence.

The immune-suppressive microenvironment and extensive tumor angiogenesis are key contributors to the high recurrence and mortality rates of oral squamous cell carcinoma (OSCC). To address these challenges, a personalized 3D-printed anti-tumor vaccine (3D-Vax-L) is developed that integrates whole tumor antigens from postoperative tissue lysates with the angiogenesis inhibitor, lenvatinib. This vaccine not only possesses inherent adjuvant properties but also mimics the functions of lymph nodes through its porous structure, facilitating the recruitment and maturation of immune cells.

Rapid lake expansion threatens the regional environment and residents on the Tibetan Plateau.

Tibetan Plateau (TP) lakes, closely linked to climate change, have expanded rapidly in recent years. Prior research has mainly focused on the changes and causes; however, the risks to local infrastructure remain uncertain. This study evaluated the 20-year impacts of lake expansion.

Uncovering chromatin accessibility dynamics in early maize endosperm and seed coat differentiation.

The early development of maize kernels plays a crucial role in determining yield and grain quality. Deciphering chromatin accessibility dynamics can further advance our understanding of tissue-specific regulatory networks. In this study, we performed an integrated multiomics analysis to explore chromatin accessibility dynamics during cell fate transitions in early kernel development.

Development and validation of a prognostic staging system for primary plasma cell leukemia.

Background: The existing risk models for multiple myeloma (MM) are suboptimal for the stratification of patients with primary plasma cell leukemia (pPCL), a rare and peculiar MM. In this study, we aimed to develop a staging system for pPCL defined as the presence of ≥ 5% circulating plasma cells (CPC) according to the new diagnostic criteria, utilizing one of the largest series of patients with pPCL.

Methods: This multicenter retrospective study included 340 patients with pPCL (the training cohort) from 25 centers nationwide in China.

TaHSP18.6 and TaSRT1 interact to confer resistance to crown rot by regulating the auxin content in common wheat.

crown rot (FCR) is one of the most serious soil-borne diseases in common wheat and has caused major wheat yield losses worldwide. Here, we identified an 18.6 kDa heat shock protein gene () through combining a transcriptome analysis and a genome-wide association study.

Exploring the Mechanism of DABCO-Catalyzed Diastereoselective Cyclization for the Synthesis of Azetidine Nitrones.

The mechanistic details of the DABCO-catalyzed diastereoselective cyclization between β-alkyl nitroolefins and alkylidene malononitriles were elucidated by using dispersion-corrected density functional theory (DFT-D3) calculations at the M06-2X/6-311G(d,p) level with SMD solvation (dichloromethane). The reaction proceeds through four consecutive steps: (i) deprotonation and Michael addition; (ii) 1,3-proton transfer; (iii) Pinner-type cyclization; and (iv) ring contraction. Our computational results demonstrate DABCO's dual catalytic role in both proton abstraction and proton shuttle mechanisms, rationalizing the observed diastereoselectivity.

Isolation and Mechanistic Investigation of the Efficient Zearalenone-Removing Strain YJ25.

Zearalenone (ZEN), a non-steroidal estrogenic mycotoxin produced by species, poses a significant threat to both human food safety and animal feed quality. In this study, we isolated a strain, designated as YJ25, from a contaminated moldy corn sample, demonstrating substantial effectiveness in removing ZEN. Our findings revealed that YJ25's ZEN removal occurs primarily through cell wall adsorption, with enzymatic degradation representing a potential mechanism.

Digital health capacity and pediatric care quality in LMICs: A large-scale analysis of 5311 health facilities.

Objective: Digital health technologies are crucial for achieving universal health coverage (UHC), especially in low- and middle-income countries (LMICs) with limited digital infrastructure. This study aimed to assess digital health capacity across multiple LMICs and evaluate its association with evidence-based practice (EBP) and patient-centered care (PCC).

Methods: We analyzed Service Provision Assessment data collected over the past decade, spanning 5311 facilities and 20,880 pediatric visits across eight LMICs.

Maternal exposures to ambient carbon particulate pollution and the risk of gestational hypertension or pre-eclampsia: a retrospective cohort study in Beijing, China, 2014-2018.

Objectives: We aimed to investigate the impact of ambient carbon particles on hypertensive disorders in pregnancy (HDP) and evaluate whether the associations differed by maternal characteristics.

Methods: A retrospective cohort study was constructed based on data from the Haidian Maternal and Child Health Hospital in Beijing, China. A total of 41 374 participants with last menstrual period dates between 2014 and 2018 were included.

A Review of -GaO Films: Fabrications and Photoelectric Properties.

Gallium oxide (GaO), as an ultra-wide bandgap semiconducting material, has attracted extensive research interest in recent years. Owing to its outstanding electrical and optical properties, as well as its high reliability, GaO shows great potential in power electronics, optoelectronics, memory devices, and so on. Among all the different polymorphs, -GaO is the second most thermally stable phase.

Phosphate-solubilizing function of PSM16 and its underlying mechanism.

Phosphorus is a crucial nutrient for plant growth, but only a limited quantity is typically accessible in the soil for plants to absorb directly. Phosphate-solubilizing bacteria (PSB) can convert inorganic phosphorus compounds into forms that are more readily usable for plant nutrition. Our previous research has verified the function of PSM16 in degrading phytic acid.

Overexpression of the eukaryotic translation initiation factor FveIF3h represses CER4/FAR3 translation and attenuates the synthesis of cuticular wax in strawberry leaves.

The cuticle layer serves as the first protective barrier against environmental biotic and abiotic stresses for plants. Although transcriptional and post-transcriptional regulation of cuticle biosynthesis genes has been extensively studied, the translational control remains poorly understood. The eukaryotic translation initiation factor (eIF3h) is a known regulator in protein translation.

Atmospheric Water Sorption-Desorption as a Pathway for Green Energy Generation.

Beyond the indispensable resource for living creatures, water has recently been explored as an eco-friendly source of energy, known as the hydrovoltaic technology. Of significant interest, the cyclic sorption/desorption of water molecules physically and chemically induces the gaseous/liquid-solid interactions with the substances, demonstrating potential for harnessing electricity. Existing efforts have focused on materials engineering and structural design of the energy generators, enhancing the generated electricity for real-life applications.