The effect of the "Tears of Life" situational puzzle board game on death anxiety among nursing students: A randomized controlled trial.

Objectives: To examine the effects of death education using a situational puzzle board game versus reflective writing on nursing students' death anxiety, self-esteem and sense of meaning in life.

Background: Pre-clinical nursing students often exhibit high death anxiety due to limited clinical experience, potentially compromising future palliative care quality. Game-based learning shows promise in nursing education, warranting investigation for death education.

Current landscape of the immunoproteasome: implications for disease and therapy.

The immunoproteasome, an inflammation-induced proteasome variant, coordinates proteostasis and adaptive immunity by replacing constitutive subunits (β1, β2, β5) with inducible counterparts (β1i, β2i, β5i). This specialization enhances antigen processing for MHC class I presentation and oxidative protein clearance. Beyond immune regulation, it critically contributes to cardiovascular, respiratory, neurodegenerative, autoimmune, retinal, and oncological pathologies through mechanisms involving NF-κB activation, mitochondrial dysfunction, and inflammatory polarization.

Enhancing symptom control in postoperative middle-aged breast cancer patients through family support: A cross-sectional study utilizing latent profile analysis and latent interaction models.

Purpose: This study aimed to classify symptom types among young and middle-aged breast cancer survivors and compare differences in family resilience, family caring, and family function across these classifications.

Methods: A cross-sectional study was conducted with 432 young and middle-aged postoperative breast cancer survivors from a hospital in Liaoning Province, China. Symptom, family caring, family functioning, and family resilience scores were collected using convenience sampling.

Dietary Urolithin A Suppresses Lung Cancer via Gut Microbiota-Mediated Autophagy Activation.

Urolithin A (UA), a gut microbiota-derived metabolite of ellagic acid, exhibits diverse biological activities. Emerging evidence suggests its anti-tumor potential, possibly mediated through gut microbiota modulation, yet its role in lung cancer remains unclear. In this study, UA dose- and time-dependently suppressed lung cancer cell proliferation.

Rab10 regulates cortical granule translocation and ZP2 dynamics for zona pellucida function during oocyte maturation.

Successful fertilization is important for early embryo development and offspring generation. Cortical granules, which are formed in the cytoplasm, are transported to the cortex to avoid polyspermy, which prevents sperm penetration into oocytes. The underlying mechanism is still largely unknown.

Nickel-Catalyzed Direct Enantioselective Alkynylation of Alkynyl Bromide with Secondary Phosphine Oxides.

Developing a transition-metal-catalyzed method for the synthesis of chiral phosphorus-centered alkynyl compounds remains a considerable challenge. Herein, we report an asymmetric coupling reaction involving nickel-catalyzed alkynyl bromides and secondary phosphine oxides, obtaining a series of chiral alkynyl phosphorus compounds with high yields (up to 74%) and excellent stereoselectivity (up to 97% ee). This method features a broad substrate scope and mild reaction conditions.

Uncovering chromatin accessibility dynamics in early maize endosperm and seed coat differentiation.

The early development of maize kernels plays a crucial role in determining yield and grain quality. Deciphering chromatin accessibility dynamics can further advance our understanding of tissue-specific regulatory networks. In this study, we performed an integrated multiomics analysis to explore chromatin accessibility dynamics during cell fate transitions in early kernel development.

Synthesis and Antifungal Activity of β-Carboline Heterocyclic Compounds.

A total of 22 β-carboline derivatives were designed and synthesized using d-tryptophan as the starting material by structurally modifying the 3 and 9 positions of β-carboline. The antifungal activity of the synthesized compounds and molecular docking studies against 5 plant pathogenic fungi were tested. The results showed that compounds , , and exhibited higher activity against than the positive control Carbendazim, with EC values of 42.

ESBL-producing Klebsiella Pneumoniae isolated from dairy cows with pneumonia: insight into their epidemiology, genetic features and antimicrobial resistance profiles.

Klebsiella pneumoniae is a common cause of respiratory tract infections in humans and animals. This study surveyed the epidemiology and analyzed the genetic and resistance characteristics, and the pathogenicity of 37 K. pneumoniae from 316 dairy cows with pneumonia in Hebei Province (north China).

Spatio-temporal expansion patterns and influencing factors in China's new local retail industry: A case study of Luckin Coffee.

with the increased penetration of the Internet, artificial intelligence, and other technologies, New Retail, an emerging commercial sector in cities, has gained significant momentum. As a new local retail brand in China, Luckin Coffee has actively explored the new retail model and rapidly captured the market. Its expansion strategy and influence mechanisms are worth exploring in depth.

Unraveling APOE4: The dual role in CNS and peripheral inflammation in Alzheimer's disease.

Inflammation in both CNS and peripheral circulation system is known to have a significant impact in AD pathogenesis. In the CNS, AD-related inflammation involves the activation of various types of immune cells, such as microglia, astrocytes, and T cells. Meanwhile, one of the hallmarks of peripheral circulation inflammation in AD is activation of T cells.

The impact of interactive motor-cognitive dual tasking on brain activation, functional connectivity, and behavioral performance in healthy adults: an fNIRS study.

Objective: This study aimed to explore how varying levels of interactive motor-cognitive dual task difficulty affect brain activation, functional connectivity (FC), and behavioral performance in healthy adults using functional near-infrared spectroscopy (fNIRS).

Methods: We recruited 28 healthy participants to perform interactive motor-cognitive dual task at three difficulty levels: easy task (ET), medium task (MT), and difficult task (DT). The tasks involved walking while simultaneously engaging in cognitive challenges.

Optimized prediction of diabetes complications using ensemble learning with Bayesian optimization: a cost-efficient laboratory-based approach.

Background And Objective: The increasing global prevalence of diabetes has led to a surge in complications, significantly burdening healthcare systems and affecting patient quality of life. Early prediction of these complications is critical for timely intervention, yet existing models often rely heavily on clinical indicators while underutilizing fundamental laboratory test parameters. This study aims to bridge this gap by leveraging the 12 most frequently tested laboratory indicators in diabetic patients to develop an optimized predictive model for diabetes complications.

Sodium Reduction Through Sensory Interactions With NaCl: Strategies and Underlying Mechanisms.

Excessive sodium intake from processed foods poses significant health risks, necessitating effective sodium-reduction strategies in the food industry. This review explores sensory interactions between sodium chloride and flavor stimuli, including taste (sweetness, sourness, bitterness, and umami), orthonasal and retronasal olfaction (e.g.

A scale for measuring graduate nursing students' academic resilience: a development and psychometric testing study.

Background: The level of academic resilience among graduate nursing students plays a critical role in determining their ability to successfully complete their studies and develop into competent advanced clinical nurses. To more accurately assess the academic resilience of graduate nursing students, this study aims to develop an academic resilience scale specifically for graduate nursing students and evaluate its psychometric properties.

Method: This study was divided into two stages to develop the academic resilience scale for graduate nursing students (ARSGNS).

Cerebral hemodynamics and functional connectivity changes in stroke patients with dysphagia under acidic taste stimulation: a preliminary study.

Background: Swallowing difficulties after a stroke are a common complication that significantly impact the quality of life of patients. The cortical activation patterns in patients with dysphagia following a stroke may be influenced by different taste stimuli, but the underlying neural mechanisms remain unclear.

Objectives: The aim of this study was to investigate the changes in brain cortical hemodynamic signals and functional connectivity in stroke patients with dysphagia during acidic taste stimulation.

Automatic Summarization of Doctor-Patient Encounter Dialogues Using Large Language Model through Prompt Tuning.

Automatic text summarization (ATS) is an emerging technology to assist clinicians in providing continuous and coordinated care. This study presents an approach to summarize doctor-patient dialogues using generative large language models (LLMs). We developed prompt-tuning algorithms to instruct generative LLMs to summarize clinical text.

ZNFX1 suppresses apoptosis-associated mRNA stability in cardiomyocyte to protect against myocardial infarction.

Cardiovascular diseases remain a growing global health burden, with myocardial infarction (MI) persisting as the leading cause of cardiovascular mortality worldwide. Zinc finger NFX1-type containing 1 (ZNFX1), an RNA helicase family member, remains relatively understudied in molecular biology and its role in cardiovascular diseases remains unclear. This study aims to explore the involvement of ZNFX1 in MI and uncover its mechanisms.

A Density Functional Theory-Based Particle Swarm Optimization Investigation of Metal Sulfide Phases for Ni-Based Catalysts.

Nickel (Ni) catalysts have numerous applications in the chemical industry, but they are susceptible to sulfurization, with their sulfurized structures and underlying formation mechanisms remaining unclear. Herein, density functional theory (DFT) combined with the particle swarm optimization (PSO) algorithm is employed to investigate the low-energy structures and formation mechanisms of sulfide phases on Ni(111) surfaces, especially under high-sulfur-coverage conditions where traditional DFT calculations fail to reach convergence. Using (3×3 ) Ni(111) slab models, we identify a sulfurization limit, finding that each pair of deposited sulfur atoms can sulfurize one layer of three Ni atoms at most (Ni:S = 3:2), with additional sulfur atoms penetrating deeper layers until saturation.

Enhancing Patient-Trial Matching With Large Language Models: A Scoping Review of Emerging Applications and Approaches.

Purpose: Patient recruitment remains a major bottleneck in clinical trial execution, with inefficient patient-trial matching often causing delays and failures. Recent advancements in large language models (LLMs) offer a promising avenue for automating and improving this process. This scoping review aims to provide a comprehensive synthesis of the emerging applications of LLMs in patient-trial matching.

High sensitivity of HIV antibody screening tests may lead to longer time to diagnosis: a Case Report.

Background: The fourth-generation human immunodeficiency virus (HIV) serology assay, which simultaneously detects the HIV-1 p24 antigen and HIV-1 antibodies, is available either in a combined format or as dual tests that differentiate between the p24 antigen and antibodies. Divergent detection methodologies require distinct confirmatory testing algorithms, which significantly impact the time to HIV infection.

Case Presentation: In this report, we present three cases where the HIV-1 p24 antigen tested reactive, while the HIV-1 antibody remained non-reactive in a dual testing scenario-despite both the combined test and the colloidal gold immunochromatographic assay (GICA) for HIV-1 antibodies yielding reactive results.

Angoroside C: A potent AMPK activator in the aqueous extract of Scrophularia ningpoensis, alleviates metabolic syndrome in db/db mice.

Background: Scrophularia ningpoensis Hemsl. (SN) is a traditional herbal medicine used for treating diabetes mellitus (DM). Previous studies indicated that the aqueous extract of SN (AESN) improves type 2 DM (T2DM) by activating AMP-activated protein kinase (AMPK), but the active components were unclear.

Semaglutide Alleviates Ovarian Oxidative Stress and Autophagy via the PI3K/AKT/mTOR Pathway in Mice with Polycystic Ovary Syndrome.

Background: Polycystic ovary syndrome (PCOS) is a typical reproductive endocrine system disease with high incidence rate among childbearing age women. Several clinical data show that glucagon-like peptide-1 receptor agonists (GLP-1RAs) might have therapeutic effects on PCOS, but the mechanisms are still unclear. Here, we aim to assess the effects of semaglutide (a weekly preparation of GLP-1RAs) on PCOS in vivo.

Utilization of simulation in undergraduate clinical nursing skills training: A cross-sectional study in China.

Aim: This study seeks to explore the challenges and experiences of nurse educators in adopting simulation in undergraduate clinical skills training in China.

Background: Despite global recognition in nursing education, simulation's role in Chinese nursing schools' clinical skills training is largely uncharted.

Design: A cross-sectional study.