Identification of serum N,N-dimethylglycine as a potential biomarker for prenatal diagnosis of congenital heart disease using HNMR and UPLC-MS/MS metabonomics.

Congenital heart disease (CHD) poses significant clinical challenges due to limitations in early prenatal diagnosis. This study aimed to identify serum metabolic biomarkers for CHD using a combined metabolomics approach. Serum samples from 55 pregnant women carrying fetuses with confirmed CHD (CHDP group) and 49 healthy controls (ZCP group) were analyzed via non-targeted HNMR metabolomics, revealing distinct metabolic profiles.

Exploring potential mid-trimester maternal serum biomarkers for fetal congenital heart disease: a retrospective case-control study.

Objective: To assess potential associations between maternal serum alpha-fetoprotein (AFP) and free human chorionic gonadotropin beta subunit (free β-hCG) levels during the mid-trimester and the occurrence of fetal congenital heart disease (CHD).

Methods: A retrospective case-control study was carried out, analyzing data from 303 pregnant women. Of these, 101 cases were diagnosed with fetal CHD (CHD group), and 202 cases were diagnosed with healthy fetuses (control group).

Personalized 3D Vaccine Integrates Immunotherapy and Antiangiogenic Therapy to Prevent Oral Squamous Cell Carcinoma Recurrence.

The immune-suppressive microenvironment and extensive tumor angiogenesis are key contributors to the high recurrence and mortality rates of oral squamous cell carcinoma (OSCC). To address these challenges, a personalized 3D-printed anti-tumor vaccine (3D-Vax-L) is developed that integrates whole tumor antigens from postoperative tissue lysates with the angiogenesis inhibitor, lenvatinib. This vaccine not only possesses inherent adjuvant properties but also mimics the functions of lymph nodes through its porous structure, facilitating the recruitment and maturation of immune cells.

Advances in nanocarrier-mediated cancer therapy: Progress in immunotherapy, chemotherapy, and radiotherapy.

Cancer represents a major worldwide disease burden marked by escalating incidence and mortality. While therapeutic advances persist, developing safer and precisely targeted modalities remains imperative. Nanomedicines emerges as a transformative paradigm leveraging distinctive physicochemical properties to achieve tumor-specific drug delivery, controlled release, and tumor microenvironment modulation.

Study on the correlation between hsCRP/HDL-C ratio and the severity and prognosis of ischemic stroke.

Objective: Inflammatory and lipid biomarkers are increasingly recognized for their role in acute ischemic stroke (AIS). This study evaluated the predictive value of composite indicators, focusing on the hsCRP/HDL-C ratio.

Methods: A prospective study included 183 AIS patients and 194 controls from Sichuan Provincial People's Hospital (July 2023-July 2024).

DNA methylation patterns and predictive models for metabolic disease risk in offspring of gestational diabetes mellitus.

Background: Gestational diabetes mellitus (GDM) is a common pregnancy complication with far-reaching implications for maternal and offspring health, strongly tied to epigenetic modifications, particularly DNA methylation. However, the precise molecular mechanisms by which GDM increases long-term metabolic disease risk in offspring remain insufficiently understood.

Methods: We integrated multiple publicly available whole-genome methylation datasets focusing on neonates born to mothers with GDM.

Laparoscopic radical gastrectomy for gastric cancer: an anatomical approach to right mesogastrium excision and its clinical significance.

Objective: Radical gastrectomy for gastric cancer involves the en-bloc resection of the primary tumor and complete excision of the mesogastrium. However, the surgical boundaries and techniques for removing lymph nodes above the pylorus during gastric cancer surgery remain unclear. We aimed to investigate a novel, standardized approach for excising the right mesogastrium in gastric cancer patients undergoing suprapyloric lymphadenectomy, focusing on surgical techniques and outcomes.

Early-onset neonatal sepsis in a Chinese maternal and child healthcare centre, 2017-2023.

Objective: Early-onset sepsis (EOS) remains an important issue in neonatal units. Characteristics of EOS from China have not been fully revealed yet. Our aim is to investigate epidemiology, microbiology and clinical feature of EOS in a Chinese maternal and child healthcare centre.

Preclinical and clinical evaluation of [Cu]Cu-PSMA-Q PET/CT for prostate cancer detection and its comparison with [F]FDG imaging.

This study aimed to develop and evaluate [Cu]Cu-PSMA-Q as a novel positron emission tomography (PET) imaging agent for prostate cancer detection, assessing its diagnostic accuracy and clinical applicability in comparison to [F]FDG PET imaging. [Cu]Cu-PSMA-Q was synthesized, purified, and subjected to comprehensive quality control. Its binding affinity, cellular uptake, and internalization were assessed in vitro using prostate-specific membrane antigen (PSMA)-positive LNCaP C4-2B cells.

A pilot study on the application of ISOBAR combined with case teaching method in pediatric resident nurses' clinical handover.

Objective: To design and evaluate the model combining ISOBAR communication tool with case teaching method, and to explore its influence on the nursing handover level, clinical work ability, nursing teaching quality and nurses' satisfaction of pediatric resident nurses.

Methods: A total of 84 pediatric resident nurses were selected by random sampling and divided into the experimental group (n = 40) and the control group (n = 44). The experimental group implemented the ISOBAR combined with case teaching method, while the control group adopted the traditional lecture method combined with case teaching method.

Construction of Bispecific T-Cell Engager Radiotracer and Its Micro-PET Evaluation in Pancreatic Cancer.

Mucin 17 (MUC17), a transmembrane mucin, is overexpressed in pancreatic cancer and is associated with tumor proliferation and metastasis. CD3 is an indispensable molecule on the surface of T lymphocytes, which is associated with T cell activation and participates in immune responses. Here, we developed a bispecific T-cell engager radiotracer, Zr-M17C3, targeting MUC17 and CD3, to enable noninvasive PET imaging of both tumor cells and T-cell infiltration in pancreatic cancer.

Selective apoptosis of tumor-associated platelets boosts the anti-metastatic potency of PD-1 blockade therapy.

Despite the transformative impact of programmed cell death protein-1 (PD-1) blockade therapy on metastatic/advanced solid tumor treatment, its efficacy is hindered by a limited response rate. Platelets play a pivotal role in tumor metastasis by shielding circulating tumor cells and secreting immunosuppressive factors. We here demonstrate that selectively inducing apoptosis in tumor-associated platelets (TAPs) using ABT-737-loaded nanoparticles (cyclic arginine-glycine-aspartate containing peptide-modified ABT-737-loaded nanoparticles [cRGD-NP@A]) enhances the anti-metastatic efficacy of the anti-PD-1 antibody (aPD-1).

Cu Radiolabeled PDGFRβ-Targeting Affibody for PET Imaging in Pancreatic Cancer.

Pancreatic cancer is a malignant solid tumor that contains a significant number of cancer-associated fibroblasts (CAFs). Clinical trials have confirmed that CAF-targeted radionuclide therapy can suppress tumor growth and extend the survival of patients; therefore, quantifying CAFs by molecular imaging of CAF biomarkers is helpful for assessing disease progression and therapeutic responses of pancreatic cancer. In our previous study, we found that platelet-derived growth factor receptor beta (PDGFRβ) was highly expressed on various fibroblast cells, and a novel affibody (Z) with highly specific binding to PDGFRβ had been developed.

Detectability of Al 18 F-NOTA-HER2-BCH PET for Nodal Metastases in Patients With HER2-Positive Breast Cancer.

Purpose: The aim of this study was to compare Al 18 F-NOTA-HER2-BCH and 18 F-FDG for detecting nodal metastases in patients with HER2-positive breast cancer on PET/CT.

Patients And Methods: In this retrospective study, 62 participants with HER2-positive breast cancer underwent both Al 18 F-NOTA-HER2-BCH and 18 F-FDG PET/CT. Participants were pathologically confirmed as HER2-positive (IHC 3+ or IHC 2+ with gene amplification on FISH).

Zinc nanoparticles from oral supplements accumulate in renal tumours and stimulate antitumour immune responses.

A successful therapeutic outcome in the treatment of solid tumours requires efficient intratumoural drug accumulation and retention. Here we demonstrate that zinc gluconate in oral supplements assembles with plasma proteins to form ZnO nanoparticles that selectively accumulate into papillary Caki-2 renal tumours and promote the recruitment of dendritic cells and cytotoxic CD8 T cells to tumour tissues. Renal tumour targeting is mediated by the preferential binding of zinc ions to metallothionein-1X proteins, which are constitutively overexpressed in Caki-2 renal tumour cells.

Acoustic Exaggeration Enhances Speech Discrimination in Young Autistic Children.

Child-directed speech (CDS), which amplifies acoustic and social features of speech during interactions with young children, promotes typical phonetic and language development. In autism, both behavioral and brain data indicate reduced sensitivity to human speech, which predicts absent, decreased, or atypical benefits of exaggerated speech signals such as CDS. This study investigates the impact of exaggerated fundamental frequency (F0) and voice-onset time on the neural processing of speech sounds in 22 Chinese-speaking autistic children aged 2-7 years old with a history of speech delays, compared with 25 typically developing (TD) peers.

Transforming Healthcare with Nanomedicine: A SWOT Analysis of Drug Delivery Innovation.

Objective: Nanomedicine represents a transformative approach in biomedical applications. This study aims to delineate the application of nanomedicine in the biomedical field through the strengths, weaknesses, opportunities, and threats (SWOT) analysis to evaluate its efficacy and potential in clinical applications.

Methods: The SWOT analysis framework was employed to systematically review and assess the internal strengths and weaknesses, along with external opportunities and threats of nanomedicine.

Circ-METTL15 stimulates the aggressive behaviors of papillary thyroid cancer cells by coordinating the miR-200c-3p/XIAP axis.

Background/aim: Papillary thyroid carcinoma (PTC) is the most common form of thyroid cancer. The critical importance of circular RNA (circRNA) in a range of cancer types has been lately recognized. However, research on the functions of circRNAs in PTC has been limited thus far.

Therapeutic effect of E-Lip-siRNA-sFlt1 on pre-eclampsia: targeted gene silencing and improved pregnancy outcomes.

To evaluate a liposome complex conjugated with anti-epidermal growth factor receptor (EGFR) antibodies for the treatment of pre-eclampsia (PE). In experiments, the transfection rate, silencing effect and cytotoxicity were determined. In the PE model, the siRNA distribution, mean arterial pressure, 24-h urine protein concentration, serum sFlt1 concentration, number of viable fetuses and placental weight were measured.

From bench to bedside: Platelet biomimetic nanoparticles as a promising carriers for personalized drug delivery.

In recent decades, there has been a burgeoning interest in cell membrane coating strategies as innovative approach for targeted delivery systems in biomedical applications. Platelet membrane-coated nanoparticles (PNPs), in particular, are gaining interest as a new route for targeted therapy due to their advantages over conventional drug therapies. Their stepwise approach blends the capabilities of the natural platelet membrane (PM) with the adaptable nature of manufactured nanomaterials, resulting in a synergistic combination that enhances drug delivery and enables the development of innovative therapeutics.

Dual antiplatelet therapy for ischemic stroke with intracranial arterial stenosis: a systematic review and meta-analysis.

Background: The safety and efficacy of dual antiplatelet therapy (DAPT) in ischemic stroke patients with intracranial artery stenosis (ICAS) remain contentious.

Aims: This study evaluates DAPT's effectiveness and safety for these patients.

Methods: This review was reported following PRISMA 2020 guidelines.

Asperuloside inhibits the activation of pancreatic cancer-associated fibroblasts via activating transcription factor 6.

Background: Pancreatic cancer-associated fibroblasts (CAFs) play a crucial role in tumor progression and immune evasion. Asperuloside (ASP) is an iridoid glycoside with potential anti-tumor properties. This study aimed to explore the molecular mechanisms of ASP on CAFs, particularly focusing on its effects on activating transcription factor 6 (ATF6), a key regulator of endoplasmic reticulum stress.