Enzymatic deamidation soy protein isolate stabilized high internal phase emulsion as a new fat substitute in meat patties.

Plant-based proteins are being explored as structuring agents to mimic the texture of animal fat in meat alternatives, contributing to the development of lipid-like structural matrices in plant-based meat products. Plant-based proteins are emerging as alternatives to animal fats, aligning with the food industry's low-fat goals. However, issues such as protein aggregation and sedimentation limit their application as fat substitutes.

Unspecific peroxygenases and P450-derived peroxygenases as green biocatalysts driven by HO for sustainable applications: A review.

Unspecific peroxygenases (UPOs) are promising biocatalysts capable of catalysing selective oxygenation of organic substrates using hydrogen peroxide (HO) as the sole oxidant under mild conditions. Sharing a broad substrate range with cytochrome P450 monooxygenases, UPOs function without costly cofactors or auxiliary proteins, making them attractive for industrial biocatalysis. However, limited heterologous expression and poor HO tolerance restrict their broader application.

Phosphatidylserine Ameliorates High-Fat Diet-Induced Obesity Through Modulating the Bile Acid Metabolism and Gut Microbiota.

Obesity has emerged as a prevalent clinical and public health concern in contemporary society, imposing significant economic and social burdens worldwide. Phosphatidylserine (PS), a functionally diverse phospholipid, demonstrates multiple biological activities. This study delves into the efficacy and underlying mechanisms of PS in mitigating high-fat diet (HFD)-induced obesity in mice.

Engineering T7 RNA polymerase-cascaded systems controlled by nisin and theophylline for protein overexpression and targeted gene mutagenesis in .

serves as an important platform for heterologous protein production, with the nisin-controlled gene expression (NICE) system being widely employed for regulated protein overexpression. However, the NICE system relies on the native RNA polymerase, which limits transcriptional efficiency, and there remains a lack of tools enabling continuous target gene mutagenesis in . In this study, we enhanced the NICE system by integrating the highly processive T7 RNA polymerase (T7RNAP) to boost protein expression.

Synergistic Probiotic-Postbiotic Therapy Ameliorates Hyperuricemia via Multi-Target Regulation of Purine Metabolism and Gut Microbiota.

Hyperuricemia (HUA), a metabolic disorder characterized by elevated serum uric acid (UA) levels, often leads to renal and hepatic complications. This study evaluated the synergistic effects of GQ01, a probiotic strain isolated from naturally fermented wolfberry, in combination with a complex (T) composed of buckwheat-fermented postbiotics, collagen peptide and multiple medicinal food blends in a murine HUA model. The combination therapy (T + GQ01) not only significantly reduced serum UA levels more effectively than T or GQ01 alone but also demonstrated superior inhibition of XOD activity and enhanced ADA activity, both of which are key regulators of purine metabolism.

Engineering Laccase from for Improved Thermostability and Its Combination with Lytic Polysaccharide Monooxygenase for Lignin Depolymerization.

Laccase from is widely used to improve lignin depolymerization. However, wild-type laccase usually lacks heat resistance, and its catalytic process is usually accompanied by the repolymerization of lignin, limiting its application. Herein, we performed site-saturation mutation on laccase, generating mutants A347H and N368L with activities increased by 2.

A Programmable Bidirectional Dynamic Switch Overcomes Reversible Isomerization Reaction for Efficient d-Allulose Biosynthesis.

d-Allulose, biosynthesized through D-fructose isomerization, was limited by thermodynamic equilibrium, resulting in low yields. Herein, we redesigned a redox-driven cascade pathway for d-allulose biosynthesis in single-cell systems, utilizing post-translational protein-level reprogramming tools (protease-based programmable OFF/ON-switch toolbox) to resolve enzyme incompatibility. This OFF/ON-switch was systematically optimized by adjusting the expression levels of -Lon, degron, and repressors, enabling the simultaneous switching of protein abundance to desired states within 2 h based on a thermosensitive regulator.

Combination of Metabolic Engineering and High-throughput Screening to Realize High-Producing Lacto--triose II in .

Lacto--triose II (LNT II) exhibits diverse biological activities. It serves as the core structural unit of complex human milk oligosaccharides (HMOs) and is an essential precursor for synthesizing other HMO derivatives. In this study, an () strain with a high capacity for LNT II production was engineered.

Construction and Optimization of a BgaR-Based Lactose Biosensor for High-Throughput Selection of Human Milk Oligosaccharide Producers.

Biosensors are crucial for high-throughput screening (HTS) of metabolite overproducers, but human milk oligosaccharide (HMO) biosensors are rarely reported. The concentrations of HMOs were evaluated by detecting the residual lactose, since lactose served as a universal substrate. In this study, a lactose biosensor was developed using the lactose-sensing transcription factor BgaR, correlating lactose concentration with strain growth.

Transfer Learning for Designing Efficient Signal Peptides to Improve the Secretion Level of Recombinant Protein in .

Signal peptides (SPs) play an essential role in determining the secretion efficiency of proteins of interest (POIs). However, the manual identification of SPs with a high secretion potential is both time-consuming and labor-intensive. Recently, many advanced machine learning (ML) techniques have emerged in biology and food research.

Triple-Probiotic-Fermented Goji ( L.) Ameliorates Metabolic Disorders Associated with Hyperuricemia in Mice.

Hyperuricemia (HUA) is a metabolic disorder characterized by excessive uric acid (UA) production and impaired excretion. Goji, as a representative medicinal food, holds significant research and development value, while probiotic fermentation technology is finding increasingly widespread applications in the functional food sector. This study developed a novel goji fermented with three probiotic strains ( CGMCC8198, LTJ28, and YR2-2) and investigated its anti-HUA effects.

Highly Efficient One-Pot Multienzyme Cascades for the Synthesis of S-Allyl-l-Cysteine from l-Serine and Allyl Mercaptan.

S-Allyl-l-cysteine (SAC) is an important bioactive substance in garlic, which has potential preventive and therapeutic effects, including antioxidant, anticancer, hepatoprotective, and neuroprotective properties. Here, a promising multienzyme cascade catalysis system comprising serine acetyltransferase (SAT) and -acetylserine sulfhydrylase B (OASS B) was established using l-serine and allyl mercaptan as substrates for the efficient synthesis of SAC. Three SAT mutants (SAT, SAT, and SAT) were designed to weaken the feedback inhibitory effect of SAC on SAT activity, with one SAT mutant achieving a SAC yield of 75.

Enhancing alkaline protease production in Bacillus amyloliquefaciens via surfactin-mediated mechanisms and metabolic engineering.

Bacillus amyloliquefaciens exhibits significant efficacy in the production of alkaline protease. Identifying the factors that trigger alkaline protease overproduction during the fermentation process provides valuable insights for enhancing the performance of alkaline protease-producing strains. Transcriptomic analysis revealed that quorum sensing (QS) plays a crucial role in regulating alkaline protease production.

Microplastic-induced alterations in growth and microecology of mulberry seedlings: Implications for sustainable forest-soil systems.

This study investigated the ecotoxicological effects of polyethylene (PE) and polylactic acid (PLA) microplastics (MPs) on mulberry growth and soil-microbe interactions through controlled pot experiments (0.1 % and 1 % concentrations). PE exposure significantly increased mulberry height by 16 % (0.

Site-specific Glycosylation via Metal-Targeted Conjugation Enhances Biochemical Functions of Bovine Lactoferrin.

Bovine lactoferrin (BLF) has attracted increasing attention due to its multiple nutritional benefits. However, the precise modification of BLF remains a significant challenge. Here, a site-specific glycosylation strategy, based on a copper assisted sequence-specific binding tag and prediction of glycosylation sites, was designed to enable the precise and controlled glycosylation of BLF in vitro.

Pickering emulsion stabilized by protein-glutaminase modified soybean isolated proteins as a new delivery system.

Plant protein-based Pickering emulsions have garnered significant attention for their natural, stable, and efficient properties. However, the aggregation and sedimentation of plant proteins have restricted their applications in the food industry. To address this challenge, the current study explored the effect of protein-glutaminase on the structural characteristics of soy protein isolate (SPI) and assessed the potential of emulsions prepared from deamidated SPI as carriers for bioactive compounds.

Explore the Contamination of Antibiotic Resistance Genes (ARGs) and Antibiotic-Resistant Bacteria (ARB) of the Processing Lines at Typical Broiler Slaughterhouse in China.

Farms are a major source of antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARB), and previous research mainly focuses on polluted soils and breeding environments. However, slaughtering is an important link in the transmission of ARGs and ARB from farmland to dining table. In this study, we aim to reveal the pollution of ARGs and ARB in the slaughter process of broilers.

Redesigning CYP109E1 for Improving Catalytic Performance in 25-Hydroxyvitamin D Synthesis Through Synergistic Enhancement of Electron Transfer and NADPH Regeneration.

P450 enzymes are promising biocatalysts and play an important role in the field of drug synthesis due to their high catalytic activity and stereoselectivity. CYP109E1 from was used to convert VD for the production of 25(OH)VD. However, the industrial production was still limited due to the low catalytic performance of CYP109E1.

Effect of exogenous protein crosslinking on the physicochemical properties and in vitro digestibility of corn starch.

Starch is a primary energy source of human diet. Its physicochemical properties and digestibility can be improved by incorporating exogenous protein. In this study, mung bean protein isolate was covalently crosslinked using transglutaminase and proanthocyanidin to create crosslinked mung bean protein isolate.

Application of multiple genomic-editing technologies in for improved enduracidin yield.

an industrial strain for enduracidin production, shows significant potential as a cellular chassis for the synthesis of novel small peptides. Targeted deletion of secondary metabolite gene clusters offers a promising strategy to enhance strain performance, but is often hampered by the lack of efficient gene editing tools. In this study, we optimized the traditional homologous recombination method by integrating selection and counter-selection markers to streamline the gene editing process, and successfully deleted gene clusters of up to 54.

Transcriptomic analysis of Bacillus licheniformis 2709 reveals the molecular mechanism of alkaline protease biosynthesis regulated by the DegS/DegU two-component system.

The DegS/DegU two-component signal transduction system (TCS), plays significant roles in a broad range of bacterial responses to the complicated environment in Bacillus subtilis. However, few efforts have been made to explore the physiological functions of DegS/DegU in alkaline protease (AprE) biosynthesis in the industrial strain Bacillus licheniformis 2709. In this study, it was found that the biosynthesis of AprE is severely hampered in degS and degU deficient mutants compared with the original strain.

LICEDB: light industrial core enzyme database for industrial applications and AI enzyme design.

Enzymes, serving as eco-friendly catalysts, are progressively supplanting traditional chemical catalysts in light industry sectors such as feed, papermaking, textiles, detergents, leather, and sugar production. Despite this advancement, the variability in the performance of natural enzymes and the fragmentation and diversity of existing data formats pose significant challenges to researchers. Furthermore, AI-driven enzyme design is limited by the quality and quantity of available data.

Development of a Cell-Free, Toehold Switch-Based Biosensor for Rapid and Sensitive Zika Virus Detection.

The need for rapid and sensitive diagnostic tools is emphasized by the significant impact of infectious diseases on global health. This study presents a cell-free biosensor utilizing toehold switch technology, combined with nucleic acid sequence-based amplification (NASBA), for high specificity and sensitivity in Zika virus detection. The toehold switch, a denovo-designed regulator of gene expression, forms the crux of our detection system, offering a versatile and programmable approach to nucleic acid-based diagnostics.

Cell-free synthesis system: An accessible platform from biosensing to biomanufacturing.

The fundamental aspect of cell-free synthesis systems is the in vitro transcription-translation process. By artificially providing the components required for protein expression, in vitro protein production alleviates various limitations tied to in vivo production, such as oxygen supply and nutrient constraints, thus showcasing substantial potential in engineering applications. This article presents a comprehensive review of cell-free synthesis systems, with a primary focus on biosensing and biomanufacturing.

[A flavin-containing monooxygenase from : characterization and application in the synthesis of -methyl-L-cysteine sulfoxide].

-methyl-L-cysteine sulfoxide (SMCO) is a non-protein sulfur-containing amino acid with a variety of functions. There are few reports on the enzymes catalyzing the biosynthesis of SMCO from -methyl-L-cysteine (SMC). In this study, the flavin-containing monooxygenase gene derived from () was heterologously expressed in BL21(DE3) and the enzymatic properties of the expressed protein were analyzed.