Exploring Europium Complex With Aggregation Induced Emission Properties for Immune Fluorescence Test Strips Applications.

Highly luminescent nanospheres have been demonstrated to enhance the sensitivity of lateral flow immunoassay (LFIA) due to their loading of numerous luminescent dyes. Herein, aggregation-induced emission (AIE) luminogens (AIEgens)-embedded nanospheres (AIENPs) with red-emissive AIE-active europium complexes were developed as signal amplification probes for LFIA for rapid and point-of-care detection of COVID-19 biomarkers, nasopharyngeal carcinoma biomarkers, and methamphetamine. Compared with the colloidal gold-based rapid and point-of-care detection approach, the AIENPs-based approach showed more than 10 times sensitivity to the target proteins and small molecules, highlighting its significant potential for pathogen- or cancer-related diseases early detection and screening, as well as its applicability in public safety monitoring.

Machine Learning-Enhanced Nanozyme Sensor Array for Accurate Multiple Quinolone Antibiotics Recognition.

The overuse of quinolone antibiotics (QNs) seriously endangers human health and the ecological environment. In this work, a copper dihydroxosulfate (Cu(OH)SO) nanosheet exhibiting notable peroxidase-like (POD) and laccase-like (LAC) activities has been developed in basic deep eutectic solvents (DES). The unique physicochemical properties of QNs allow them to enhance the POD activity of Cu(OH)SO, and with the extension of reaction time, this enhancement gradually intensifies.

Engineering Laccase from for Improved Thermostability and Its Combination with Lytic Polysaccharide Monooxygenase for Lignin Depolymerization.

Laccase from is widely used to improve lignin depolymerization. However, wild-type laccase usually lacks heat resistance, and its catalytic process is usually accompanied by the repolymerization of lignin, limiting its application. Herein, we performed site-saturation mutation on laccase, generating mutants A347H and N368L with activities increased by 2.

Machine learning assisted nanozyme sensor array for accurate identification and discrimination of flavonoids in healthy tea.

Identifying flavonoids in herbs is of great significance for elucidating their biological activity and pharmacological effects. However, distinguishing and detecting multiple flavonoids simultaneously remains a challenge. Here, an innovative citric acid-Cu (CA-Cu) nanozyme with peroxidase mimic (POD) and laccase mimic (LAC) activities was successfully synthesized.

MTPSol: Multimodal Twin Protein Solubility Prediction Architecture Based on Pretrained Models.

In the process of mining and de novo designing of new enzymes, the solubility of proteins is one of the key factors determining the efficiency of their functional expression. The development of solubility prediction algorithms is important for reducing experimental costs and enhancing the success of protein engineering. However, only a small number of studies have involved the input of protein structural information, which extremely limited the models' accuracy and generalization.

Histidine-engineered Cu-BTC nanozyme with enhanced laccase-like activity combining the machine learning for precise recognition of Beta-lactam antibiotics.

Although nanozyme sensor arrays can simultaneously recognize multiple target substances, they are currently rarely used for identifying Beta-lactam antibiotics (BLs). This may be due to the lower catalytic performance of some nanozymes in practical applications, which further limits the detection performance of nanozyme sensor arrays. Therefore, developing highly active nanozymes is particularly important.

Application of multiple genomic-editing technologies in for improved enduracidin yield.

an industrial strain for enduracidin production, shows significant potential as a cellular chassis for the synthesis of novel small peptides. Targeted deletion of secondary metabolite gene clusters offers a promising strategy to enhance strain performance, but is often hampered by the lack of efficient gene editing tools. In this study, we optimized the traditional homologous recombination method by integrating selection and counter-selection markers to streamline the gene editing process, and successfully deleted gene clusters of up to 54.

LICEDB: light industrial core enzyme database for industrial applications and AI enzyme design.

Enzymes, serving as eco-friendly catalysts, are progressively supplanting traditional chemical catalysts in light industry sectors such as feed, papermaking, textiles, detergents, leather, and sugar production. Despite this advancement, the variability in the performance of natural enzymes and the fragmentation and diversity of existing data formats pose significant challenges to researchers. Furthermore, AI-driven enzyme design is limited by the quality and quantity of available data.

Schwannoma in the lower inner quadrant of the right breast in a male patient: A rare case report.

Rationale: Lower inner-quadrant breast schwannomas are exceedingly rare, with no reports of their occurrence in male patients in the literature. In this report, we describe a male patient with a schwannoma in the lower inner quadrant of the right breast.

Patient Concerns: A 26-year-old man presented at our hospital with a 6-month history of a lump in the right breast and reported pain in the area 3 days prior to presentation.

Ergothioneine exerts neuroprotective effects in Parkinson's disease: Targeting α-synuclein aggregation and oxidative stress.

Ergothioneine (EGT) is a natural dietary antioxidant derived from certain edible mushrooms, commonly used as a food additive and supplement, but its effects on Parkinson's Disease (PD) are still unclear. The accumulation of α-synuclein (α-syn) plays a pivotal role in the pathogenesis and development of PD. Here, this study demonstrated that EGT effectively inhibits α-syn aggregation, disrupts mature fibers, and reduces associated cytotoxicity and oxidative stress.

Machine learning assisted multi-signal nanozyme sensor array for the antioxidant phenolic compounds intelligent recognition.

Identifying antioxidant phenolic compounds (APs) in food plays a crucial role in understanding their biological functions and associated health benefits. Here, a bifunctional Cu-1,3,5-benzenetricarboxylic acid (Cu-BTC) nanozyme was successfully prepared. Due to the excellent laccase-like behavior of Cu-BTC, it can catalyze the oxidation of various APs to produce colored quinone imines.

Ultrasound-Assisted Enzymatic Extraction of Polysaccharides from : Optimization, Structural Characterization, and Inhibition of α-Synuclein Aggregation.

This study optimized ultrasound-assisted enzymatic (UAE) extraction of TMP ( polysaccharide) through response surface methodology. The optimal conditions included complex enzyme comprising 1.15% cellulase, 0.

A Molecular Logic Gate Enables Unconventional Super-resolution Same-Day Imaging of Lysosome Membrane in Live Cells.

Lysosomes are acidic membrane-bound organelles that aid digestion, excretion, and cell renewal. The lysosomal membranes are essential for maintaining lysosomal functions and cellular homeostasis. In this work, we developed a molecular "NOR" logic gate, , by introducing malachite green into the spirocyclic rhodamine.

Brazilin-Rich Extract from L. Attenuated the Motor Deficits and Neurodegeneration in MPTP/p-Induced Parkinson's Disease Mice by Regulating Gut Microbiota and Inhibiting Inflammatory Responses.

Parkinson's disease (PD) is a complicated neurological disease with an unclear pathogenesis. However, dysregulation of gut microbiota and inflammation response play crucial roles in the progression of PD. L.

Tetrahydrofolic acid accelerates amyloid fibrillization, decreases cytotoxic oligomers and suppresses their toxicity.

Soluble cytotoxic oligomers produced during the fibrillation of both α-synuclein (αS) and amyloid-β protein (Aβ) are key pathogenic factors in Parkinson's disease (PD) and Alzheimer's disease (AD). Reducing toxic oligomers by regulating the aggregation process of αS and Aβ is an important strategy for the treatment of PD and AD. Herein, tetrahydrofolic acid (THF) is found to accelerate amyloid fibrillization, decreases cytotoxic oligomers and suppresses their toxicity.

Dual-mode colorimetric and chemiluminescence aptasensor for organophosphorus pesticides detection using aptamer-regulated peroxidase-like activity of TA-Cu.

The residues of organophosphorus pesticides (OPs) in food pose a huge threat to human health. Therefore, the development of detection methods with simple design and high sensitivity is urgently needed. Here, a colorimetric/chemiluminescence (CL) dual-mode aptasensor strategy with high selectivity and sensitivity for detecting Parathion-methyl (PM) was designed based on aptamer-regulated nanozyme activity.

9-Methylfascaplysin Prevents Neuroinflammation and Synaptic Damage via Cell-Specific Inhibition of Kinases in APP/PS1 Transgenic Mice.

Background: Alzheimer's disease (AD) is a leading neurodegenerative disorder without effective treatments. The nonlinear dynamic nature of AD pathophysiology suggested that multiple pharmacological actions of anti-AD drugs should be elucidated. 9-Methylfascaplysin (9-MF) was previously designed and synthesized as a novel anti-AD candidate.

Enzymes in Food Industry: Fermentation Process, Properties, Rational Design, and Applications.

Enzymes play a vital role in the food industry, where they act as natural catalysts to improve production processes, enhance food quality, and increase efficiency [...

Machine Learning-Based Nanozyme Sensor Array as an Electronic Tongue for the Discrimination of Endogenous Phenolic Compounds in Food.

The detection of endogenous phenolic compounds (EPs) in food is of great significance in elucidating their bioactivity and health effects. Here, a novel bifunctional vanillic acid-Cu (VA-Cu) nanozyme with peroxidase-like and laccase-like activities was successfully prepared. The peroxidase mimic behavior of VA-Cu nanozyme can catalyze 3,3',5,5'-tetramethylbenzidine (TMB) to generate oxidized TMB (oxTMB).

Sustainable Production of Oligosaccharides via Enzymatic Hydrolysis: A Review on Ulvan Lyase.

Ulvan is a water-soluble sulfated polysaccharide extracted from the green algae cell wall. Compared with polysaccharides, oligosaccharides have drawn increasing attention in various industries due to their enhanced biocompatibility and solubility. Ulvan lyase degrades polysaccharides into low molecular weight oligosaccharides through the -elimination mechanism.

CuCl(OH) nanozyme-based colorimetric sensor array for phosphates discrimination and disease identification.

The identification of phosphates holds significant importance in many physiological processes and disease diagnosis, and traditional detection techniques struggle to simultaneously detect and distinguish phosphates. The complexity of synthesizing sensing units restricts the construction of sensor arrays as well. In this study, a bifunctional dicopper chloride trihydroxide (CuCl(OH)) nanozyme with conspicuous laccase- and peroxidase-like activities has been synthesized in basic deep eutectic solvents (DES).

Cu(OH)NO nanozyme sensor array for the discrimination of multiple sulfides in food.

In the food industry, sulfides are commonly used as preservatives and flavor regulators. However, long-term excessive intake of sulfides can lead to serious health problems. Therefore, developing efficient sulfide detection methods is particularly important.