98%
921
2 minutes
20
The Microbiome Protocols eBook (MPB) serves as a crucial bridge, filling gaps in microbiome protocols for both wet experiments and data analysis. The first edition, launched in 2020, featured 152 meticulously curated protocols, garnering widespread acclaim. We now extend a sincere invitation to researchers to participate in the upcoming 2nd version of MPB, contributing their valuable protocols to advance microbiome research.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11170964 | PMC |
http://dx.doi.org/10.1002/imt2.182 | DOI Listing |
Nutr J
September 2025
Department of Geriatric, The First Hospital of China Medical University, No. 155 Nanjing North Street, Heping Ward, Shenyang, 110001, China.
Objective: This study analyzed data from the US population to examine how oral microbiome diversity and diet quality individually and synergistically affect frailty.
Methods: This study included 6,283 participants aged 20 years or older from the 2009-2010 and 2011-2012 NHANES cycles. A frailty index (FI) consisting of 36 items was developed, with items related to nutritional status excluded.
J Microbiol Methods
September 2025
Dynamics of Respiratory Infections Group, Helmholtz Centre for Infection Research-HZI Braunschweig, Braunschweig, Germany; Department of Respiratory Medicine and Infectious Diseases, Hannover Medical School, German Center for Lung Research (DZL), BREATH, Hannover, Germany.
Purpose: The accuracy of oral microbiome research depends significantly on specimen sampling protocols, as well as their storage and preservation. Traditional methods, such as freezing, may not only involve logistical hurdles but can also impact the quality of microbial data, leading to difficulties in the comparability between different studies. This study evaluates the effectiveness of the room temperature nucleic acid preservation protocol using DNA/RNA Shield buffer as compared to standard freezing in preserving oral microbial communities over the course of 7 days.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
Academy of Advanced Carbon Conversion Technology, Huaqiao University, Xiamen, 361021, China; Fujian Provincial Key Laboratory of Biomass Low-Carbon Conversion, Huaqiao University, Xiamen, 361021, China; College of Chemical Engineering, Huaqiao University, Xiamen, 361021, China. Electronic address: l
Over recent decades, the indiscriminate use of antibiotics in animal production to enhance product quality and maximize economic returns has raised critical concerns. However, antibiotic misuse has led to the development of antimicrobial resistance in livestock and poses substantial health risks to humans through drug residue accumulation. In response, nations globally have progressively implemented bans on antibiotic inclusion in animal nutrition, redirecting scientific attention toward antibiotic-free feed additives that maintain or enhance animal health performance.
View Article and Find Full Text PDFCurr Probl Cardiol
September 2025
Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston.
Nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM) represent interconnected metabolic disorders with multifaceted etiology, demonstrating bidirectional relationships and pronounced associations with cardiovascular diseases (CVDs). Despite extensive research, significant knowledge gaps persist regarding the temporal progression of these comorbidities, optimal screening strategies for high-risk populations, and personalized therapeutic approaches targeting the hepatic-cardiac-metabolic axis simultaneously. Current literature lacks a comprehensive analysis of phenotypic heterogeneity within NAFLD-T2DM-CVD clusters and fails to address sex-specific and ethnic variations in disease progression patterns adequately.
View Article and Find Full Text PDFPLoS Negl Trop Dis
September 2025
Microbiota of Insect Vectors Group, Institut Pasteur de la Guyane, Cayenne, French Guiana.
Wolbachia, an endosymbiotic bacterium infecting a wide array of invertebrates, has gained attention for its potential in vector control. Its capacity to colonise host populations primarily relies on vertical transmission and reproductive manipulation in arthropods. This endosymbiont is additionally mutualistic in some hosts, across several Wolbachia supergroups; notably, in nematodes and, as recently demonstrated, in planthoppers and bedbugs, it functions as an essential nutritional symbiont by providing vitamins to its host.
View Article and Find Full Text PDF