Adaptive Evolution of Enhances Saline-Alkali Resistance for High-Performance Concrete Crack Repair via MICP.

Microbially induced calcium carbonate precipitation (MICP) has emerged as a research focus in concrete crack remediation due to its environmental compatibility and efficient mineralization capacity. The hypersaline conditions of seawater (average 35 g/L NaCl) and alkaline environments (pH 12) within concrete cracks pose significant challenges to the survival of mineralization-capable microorganisms. To enhance microbial tolerance under these extreme conditions, this study employed a laboratory adaptive evolution strategy to successfully develop a strain demonstrating tolerance to 35 g/L NaCl and pH 12.

A Strictly Inducible and Orthogonal Dre-rox System for Precise and Markerless Genome Editing in .

Site-specific recombination enables precise and modular genome engineering in microbial systems. In , Cre is the most commonly used site-specific recombinase (SSR) and has been widely applied in genome engineering. Developing SSRs with comparable performance to Cre that can also function orthogonally would significantly expand the genome engineering toolkit.

Characterization and Application of a Thermostable HAD Phosphatase From Thermophilibacter mediterraneus for Glucosamine Production.

Glucosamine (GlcN), a high-value nutraceutical, is currently produced via environmentally detrimental chitin hydrolysis or inefficient microbial fermentation. While acidic hydrolysis of crustacean chitin raises environmental and allergen concerns, microbial fermentation faces challenges in strain engineering and byproduct formation. One-pot production of GlcN from maltodextrin by an in vitro synthetic enzymatic biosystem (ivSEB) containing glucosamine 6-phosphate phosphatase, which dephosphorylates glucosamine 6-phosphate (GlcN6P) to GlcN, was developed recently.

A Novel Polysaccharide from Blackened Jujube: Structural Characterization and Immunoactivity.

Previously, research adopted an ultrasound-assisted extraction method to isolate crude polysaccharide from blackened jujube, followed by preliminary structural identification of the purified polysaccharide (BJP). This manuscript analyzed the accurate structure and immunomodulatory activity of BJP. Further structural identification indicated that BJP was mainly composed of →3)-α-L-Ara-(1→, →3,5)-α-L-Ara-(1→, →3)-β-D-GalA-(1→, →2,4)-β-D-Gal-(1→, →4)-β-D-GalA-(1→, →3)-α-L-Rha-(1→ and →3,4)-α-L-Rha-(1→.

Decoding the Molecular Mechanisms of Menthol Isomer Perception Based on Computational Simulations.

The flavor characteristics, perception, and molecular mechanisms of eight menthol isomers were investigated by sensory analysis combined with computational simulations. The sensory analysis results show significant differences in the odor profiles of the different menthol isomers. Among them, L-menthol shows a pleasant, sweet, and mint-like odor with a distinct freshness and no off-flavors, whereas the remaining seven isomers were interspersed with negative odors (musty, herbal, or earthy aromas).

Mung Bean Starch-Derived Fermented Liquid Alleviates Constipation via 5-HT Modulation and Gut Microbiota Regulation: An In Vivo Study.

Background: Constipation is a common gastrointestinal disorder with a significant impact on quality of life.

Methods: Constipation was induced in male ICR mice via 25% cotrimoxazole gavage (20 mL/kg/day for 7 days). Mice were divided into prevention (pre-MBSFL), treatment (MBSFL), and control groups.

Fabrication of surface molecularly imprinted electrochemical sensor based on VCT MXene heterogeneous functionalized Au-PtRu nanoalloy for the sensitive detection of chlortetracycline in food.

A novel molecularly imprinted electrochemical sensor (MIECS) is successfully prepared for the chlortetracycline (CTC) determination in food, based on a gold‑platinum ruthenium/vanadium carbide (Au-PtRu/VCT) nanocomposite integrated with a molecularly imprinted polymer (MIP) film. The incorporated nanocomposite, featuring excellent electron transport properties, enhances the current response owing to the synergistic effect between the Au-PtRu nanoalloy (NA) and VCT MXene. Following a one-step electropolymerization process and the efficient removal of template molecules, imprinted cavities are formed on the imprinted electrode (MIP/Au-PtRu/VCT/GCE) surface, enhancing the targeted recognition of CTC molecules.

Use of thermal coagulation of egg white protein for lignin removal from high-temperature hydrolysate to promote proliferation of Bifidobacterium adolescentis.

In this study, the effectiveness of thermal coagulation of egg white proteins (EWPs-TC) procedure in removing lignin from high-temperature corn stalk hydrolysate (CSH) and its effect on Bifidobacterium adolescentis proliferation in treated hydrolysate were investigated. The results show that the utilization of EWPs-TC technique led to the removal of 91.87 % of colloidal lignin and 21.

Effect of dielectric barrier discharge cold plasma on the thin sheet of bean curd during storage: Improve the physical properties, flavor composition, and microbial safety.

Recent advances in soy product processing have driven the exploration of innovative methods to enhance the preservation quality of soy products. This study introduces dielectric barrier discharge cold plasma (CP) as a non-thermal processing technique to suppress the proliferation of microorganisms during the storage of thin sheet bean curd (TSBC) and to mitigate the beany odor associated with it. Flavor analysis revealed that the beany odor in TSBC treated with cold plasma decreased by 55.

Conductive Fiber-Based Flexible Sensing Devices: Material, Structure, and Application.

In recent years, flexible sensor devices have demonstrated significant potential for applications in basic science, healthcare, and health monitoring. Fiber-based sensing devices have garnered considerable attention due to their high performance, wearing comfort, potential for mass production, and broad range of applications. Material and structure are the two primary factors that influence the performance of fiber sensor devices.

Engineering Xylose Metabolism and Coutilization with Glucose in for Efficient Microbial Cell Factories.

Xylose, an important sugar component of lignocellulosic biomass, is often inefficiently utilized due to limitations in its transport and metabolic pathways. This study aimed to enhance the xylose metabolism in through metabolic engineering and transcriptional regulation strategies. First, the xylose assimilation pathway was constructed by the heterologous expression of xylose isomerase (XylA) and xylulose kinase (XylB), which improved the strain's xylose metabolic capability.

Ginkgo biloba leaf polysaccharide dual-network hydrogel films: Decoding structure-function relationship for sustainable strawberry shelf-life extension.

Strawberries, prone to pathogen invasion and mechanical damage, require advanced preservation solutions. We developed a carboxymethyl chitosan/sodium alginate/ginkgo biloba polysaccharide (CMCS/SA/GBLP) composite hydrogel film (CSP) via covalent cross-linking, pioneering GBLP as a dual-functional structural modulator in food packaging. Structural analyses revealed GBLP enhances cross-linking through carboxyl with -NH coordination, densifying the 3D network, while conferring excellent antimicrobial and antioxidant properties.

Chemo- and Regioselective Sequential Addition/[2,3]-Sigmatropic Rearrangement Reaction of Vinylsulfonium Salts with -Heterocycles: Access to Functionalized Benzylic Thioethers.

An efficient base-mediated sequential Michael addition/[2,3]-sigmatropic rearrangement between vinylsulfonium salts and -nucleophiles has been developed, leading to a series of functionalized benzylic thioethers in 52-95% yields with high chemoselectivity, regioselectivity, and atom economy. The protocol enables mild conditions, is easy to manipulate, and exhibits a wide substrate range with good functional group tolerance. The viability of the method is also illustrated by gram-scale synthesis and further modification of target products and drug molecules, making it a valuable tool for drug discovery.

Environmental factors influence on phytoplankton community distribution in the eastern Indian Ocean.

To study the spatial distribution and composition of phytoplankton taxa in the eastern Indian Ocean, a total of 52 sampling stations were set up for sampling during 10 April to 13 May 2016. The HPLC-CHEMTAX technique was employed to analyze photosynthetic pigments and phytoplankton community structure. Based on vertical distribution patterns observed, phytoplankton was divided into three categories.

Synergistic Anti-Inflammatory Effects of Pomegranate Peel-Hawthorn Combinations in Ulcerative Colitis: Network Pharmacology Prediction and Experimental Validation.

Ulcerative colitis (UC) is a chronic inflammatory bowel disease characterized by complex pathogenesis involving dysregulated immunity and gut microbiota imbalance, demanding innovative therapeutic strategies. This study investigates the synergistic therapeutic potential of pomegranate peel-hawthorn combinations and their active constituents (ellagic acid and maslinic acid) through an integrative approach combining network pharmacology, in vitro/in vivo experiments, and gut microbiota analysis. Network pharmacology identified 61 shared therapeutic targets ( < 0.

Recent Advances in Photoelectrochemical Biosensors for Detection of Mycotoxins and Marine Biotoxins in Food: Identification and Signal Amplification Strategy.

Mycotoxins and marine biotoxins are highly toxic metabolites produced by fungi and phytoplankton, respectively. They can enter the body of humans and animals through feed or food, causing acute or chronic toxicity on the endocrine and nervous systems, which eventually pose a serious threat to humans and animals' life. Development of inexpensive, fast, specific, and sensitive analytical methods for mycotoxin and marine biotoxin detection in foodstuffs and the environment is of great importance.

Biomechanical study of myogenic factors in the contracture period of traumatic elbow joint.

Background: Elbow contracture is a common complication following elbow trauma. Soft tissue fibrosis is an important cause of exogenous joint contracture. The most effective non-surgical method for preventing and treating elbow contracture is stretching exercise.

High-Quantum-Yield Carbon Nitride Quantum Dots via Water Vapor Intercalation-Exfoliation for Efficient Tetracycline Hydrochloride Degradation.

Graphitic carbon nitride (g-CN) has demonstrated potential applications in addressing energy shortages and preserving water ecosystem stability due to its high efficiency in degrading antibiotics. However, the mediocre catalytic performance of bulk g-CN is attributed to inefficient carrier transport and a high complexation rate of photogenerated electron-hole pairs. Herein, a method for fabricating carbon nitride quantum dots (CNQDs) via water vapor intercalation-exfoliation during high-temperature thermo-polymerization of g-CN is proposed.

Structure characterization and construction analysis of a calcium-vitamin D co-delivery aqueous solution system based on α-cyclodextrin/casein phosphopeptide.

A aqueous co-delivery system for calcium ions (Ca) and vitamin D (VD) was developed to achieve their co-loading and synergistic release. The quaternary complex α-cyclodextrin-VD@casein phosphopeptide‑calcium chelate (α-CD-VD@CPP-Ca), prepared from α-CD-VD and CPP-Ca, exhibited a particle size of 419.46 ± 1.

CRISPR-based one-pot detection: A game-changer in nucleic acid analysis.

The CRISPR/Cas system, originally developed as a gene-editing tool, has rapidly emerged as a powerful platform for nucleic acid detection due to its remarkable specificity, programmability, and robust trans-cleavage activity. While conventional CRISPR-based assays typically require separate amplification and detection steps, introducing complexity, manual intervention, and contamination risks. Recent innovations have led to the development of one-pot detection strategies that integrate target amplification with Cas-mediated signal generation within a single reaction vessel.

Engineering T7 RNA polymerase-cascaded systems controlled by nisin and theophylline for protein overexpression and targeted gene mutagenesis in .

serves as an important platform for heterologous protein production, with the nisin-controlled gene expression (NICE) system being widely employed for regulated protein overexpression. However, the NICE system relies on the native RNA polymerase, which limits transcriptional efficiency, and there remains a lack of tools enabling continuous target gene mutagenesis in . In this study, we enhanced the NICE system by integrating the highly processive T7 RNA polymerase (T7RNAP) to boost protein expression.

Effect of egg white peptides‑selenium chelate against APAP-induced damage in HepG2 cells and C. elegans via Nrf2 signaling.

Overdose of acetaminophen (APAP) can cause severe liver injury, and selenium chelating peptide is a promising treatment strategy. This study optimized the chelation process of sodium selenite with egg white protein-hydrolyzed peptides and obtained two highly active peptides (WHCSeMAQ and FMSeCSeFFV) after ultrafiltration, LC-MS and batch docking screening, which could bind to Keap-1 via hydrogen and hydrophobic bonds, promoting Nrf2 release. The two peptides attenuated APAP-induced liver injury in HepG2 cell by modulating the Nrf2 pathway, enhancing the activities of antioxidant enzyme activities GSH-Px, CAT, and SOD, and decreasing ROS and MDA levels.

Ultrasensitive, portable and multiplexed molecular detection of pathogenic bacteria in a cross-validating manner via Argonaute-triggered and reverse-phase enhanced fluorescent lateral flow bioassay.

Foodborne illnesses and contamination are becoming increasingly severe global public health issues. In recent years, the resistance of pathogenic bacteria has increased significantly, which makes it particularly urgent to develop advanced detection technologies beyond traditional methods. PfAgo (Pyrocus furiosus Argonaute), a novel programmable nuclease with specific sequence targeting and cleavage capabilities, offers significant advantages over traditional CRISPR diagnostics (CRISPR-Dx) in molecular detection, particularly in flexible design and multiplexed detection.

Fabrication of sodium alginate gel beads encapsulating microcapsules via TGase-catalyzed crosslinking of gelatin in the outer aqueous phase of probiotics-loaded W/O/W emulsions.

Background: Probiotics are susceptible to both internal and external environments.

Methods: Therefore, in this study, we developed two probiotic delivery systems comprising transglutaminase (TGase)-crosslinked water-in-oil-in-water (W/O/W) emulsion microcapsules and sodium alginate gel beads to enhance gastrointestinal resistance and storage stability.

Results: A W/O/W emulsion was fabricated via a two-step emulsification process.

Base-Mediated Substrate-Dependent [2 + 3] and [3 + 3] Annulation of Bisnucleophiles: Enabling the Modular Synthesis of Functionalized Spirocyclopentane Oxindoles and Tetrahydro-α-carbolines.

Herein, we represented two facile and efficient approaches for the synthesis of functionalized spirocyclopentane oxindoles and tetrahydro-α-carbolines from different bisnucleophiles with 3-iodo-2-iodomethyl-1-propene via [2 + 3] and [3 + 3] annulation. These methods exhibit good functional group tolerances, simple conditions, easily available start materials, and moderate to good yields.