Correction: Li et al. Whole-Genome Sequence Analysis of and Functional Validation of , a Key Gene for γ-Aminobutyric Acid Synthesis. 2024, , 862.

In the original publication [...

Autoimmunity and hyperactivation of immune responses by HAE-FLS2 chimera.

Plants employ cell surface receptors to perceive extracellular signals and initiate appropriate cellular responses, thereby regulating diverse physiological processes. The well-characterized leucine-rich repeat receptor-like kinases (LRR-RLK) type receptor FLAGELLIN-SENSITIVE 2 (FLS2) functions as a pattern recognition receptor (PRR) that specifically detects bacterial flagellin, activating downstream responses including MAPK signaling and ROS burst. Here, we report that engineered expression of a chimeric receptor combining the N-terminal extracellular and transmembrane domains of HAE with the cytosolic kinase domain of FLS2 (HAE-FLS2) leads to excessive activation of plant immune responses, as evidenced by dwarfism, enhanced flg22-induced ROS burst and MAPK activation, and upregulated expression of defense-related genes in transgenic plants.

Hyaluronic acid-based composite hydrogels embedded with core-shell microgels with properties of mucosal adhesion and combined drug administration for chemoradiotherapy induced oral mucositis.

Chemoradiotherapy-induced oral mucositis (CIOM), characterized by high incidence and delayed healing, severely impairs patient recovery and quality of life. Current treatments face challenges due to poor wet adhesion, dynamic oral environments, and lack of multiple active substances for mucosal repair. This situation makes an urgent need for therapeutic platforms that combine robust mucosal adhesion with multifunctionality.

Nitric oxide-driven copper homeostasis and osteogenesis in cranial defect regeneration using l-arginine-loaded HKUST-1.

Copper plays a multifaceted role in bone tissue engineering, including regulating osteogenic and chondrogenic differentiation, exhibiting antibacterial activity, and enhancing mechanical properties. However, excessive copper induces a distinctive form of cell death, termed cuproptosis which compromises the biocompatibility of copper-based biomaterials. Cuproptosis relies on oxidative phosphorylation and can be inhibited by the Warburg effect, which is the characteristic of aerobic glycolysis in tumor cells.

trans-Configured Ligands Boost Spin Crossover to Room Temperature in Mononuclear Fe(II) Complexes.

Seven new mononuclear complexes [Fe(aqin)(NCS)] (1), [Fe(aqin)(NCSe)] (2), [Fe(aqin)(NCBH)] (3), [Fe(2-pic)(NCS)] (4), [Fe(2-pic)(NCSe)] (5), [Fe(2-pic)(NCBH)] (6), [Fe(tpa)(NCBH)] (7), [aqin = 8-aminoquinoline, 2-pic = 2-picolylamine, tpa = tris(2-pyridylmethyl)amine)] are investigated. Structurally, except for 3, which affords a trans-configuration, all the complexes adopt a cis-configuration. Their propensity to crystallize without any solvent molecules facilitates the study of the influence of the "pure" ligand on spin-crossover (SCO) characteristics.

Base-Mediated Substrate-Dependent [2 + 3] and [3 + 3] Annulation of Bisnucleophiles: Enabling the Modular Synthesis of Functionalized Spirocyclopentane Oxindoles and Tetrahydro-α-carbolines.

Herein, we represented two facile and efficient approaches for the synthesis of functionalized spirocyclopentane oxindoles and tetrahydro-α-carbolines from different bisnucleophiles with 3-iodo-2-iodomethyl-1-propene via [2 + 3] and [3 + 3] annulation. These methods exhibit good functional group tolerances, simple conditions, easily available start materials, and moderate to good yields.

Kinetic and quantitative analysis of [F]SMBT-1 PET imaging for monoamine oxidase B.

Background And Objective: In neuroinflammation, activated astrocytes, called reactive astrocytes, highly express monoamine oxidase B (MAO-B). [F]SMBT-1 is a novel PET tracer developed for imaging neuroinflammation, with highly selective binding to MAO-B. The quantification method for [F]SMBT-1 PET imaging has not been established, although some human studies using [F]SMBT-1 PET imaging have already been conducted.

A novel peptide 66CTG stabilizes Myc proto-oncogene protein to promote triple-negative breast cancer growth.

Triple-negative breast cancer (TNBC) is the most malignant subtype of breast cancer that lacks reliable targets for diagnosis and therapy. Non-coding RNA (ncRNA)-encoded products hold promise for addressing this unmet need. By analyzing the reported ribosomal RNA sequencing data, combined with the TCGA, ORFfinder, SmProt databases, we identified CDKN2B-AS1, a TNBC-upregulated lncRNA encoding a 66-amino-acid peptide via CUG-initiated translation.

is a novel functional gene associated with lipid metabolism in BXD recombinant inbred population.

Aim: The dysregulation of hepatic lipid metabolism is closely associated with dyslipidemia. Previous research suggested that Hepatic may play a role in regulating lipid metabolism. This research aims to elucidate the function of MORC4 in hepatic lipid metabolism, thereby improving the understanding of the molecular mechanisms underlying lipid metabolism disorders.

Survival and Morphological Changes of Spores Co-Exposed to Antibiotics and Simulated Gastrointestinal Fluids: Implications for Antibiotic Stewardship.

Probiotics are often advised to be taken separately from antibiotics due to their sensitivity to antibiotic activity and gastrointestinal (GI) stress. However, spores, as next-generation probiotics, may withstand concurrent use with antibiotics due to their unique structural adaptations. This study systematically evaluated the survival rates and morphological changes of spores exposed to 10 clinically relevant antibiotics in simulated gastric/intestinal fluids, exploring their feasibility for co-administration with antibiotics.

Increased maternal exercise of moderate intensity improves pregnancy outcomes of gestational diabetes mellitus patients through maintaining the balance of the gut microbiota.

Background: Exercise therapy can reduce insulin resistance during pregnancy and improve glucose tolerance in women with gestational diabetes mellitus (GDM), leading to better pregnancy outcomes. This study aimed to investigate the effects of different exercise levels on GDM from the perspective of gut microbiota.

Methods: Ninety patients with GDM were enrolled and divided into two groups: the L ( = 50) and the M ( = 40) groups.

Characterization and Functional Analysis of a Novel Fungal Immunomodulatory Protein Gene from in B16-F10 Mouse Melanoma Cells.

, a newly identified species from the Tibetan Plateau, has been mainly studied for its polysaccharides and triterpenoids, with no prior reports on fungal immunomodulatory proteins (FIPs). This study explores the biological activity of FIP-gle2, cloned from and expressed in . The effects and mechanisms of recombinant FIP-gle2 (rFIP-gle2) on cell activity and melanin synthesis in mouse melanoma B16-F10 cells were investigated in vitro.

Multifactorial Analysis of Trueness in Computer-Assisted Implant Surgery: A Retrospective Study.

Objectives: To investigate the clinically acceptable deviation threshold and the factors associated with trueness across three computer-assisted implant surgery (CAIS) approaches: static CAIS (s-CAIS), dynamic CAIS (d-CAIS), and robotic CAIS (r-CAIS).

Materials And Methods: A total of 314 implants in 194 patients were retrospectively included, with preoperative and postoperative CBCT scans collected. Outcome measures included platform, apex, and angular deviations, which were assessed by registering postoperative CBCT scans to preoperative implant plans using a jawbone matching technique.

Study on the scale dependence of the spatial distribution pattern of ecosystem service value - a case study of Gangu County, China.

This article aims to screen out the critical and optimal scales of the total value and spatial differentiation of ecosystem service value (ESV) to improve the accuracy and reliability of the ESV assessment results. On the basis of LULC data form 2000 to 2005, 2010, 2015, and 2020, we analyzed and explored the variation in the total value of ESVs and its critical scales in Gangu County by using the equivalent factor method and nonparametric tests. The optimal grid scale for the spatial differentiation of ESV is determined with the help of the method of coefficient of variation and Moran index.

Supplementing with Vitamin D during Pregnancy Reduces Inflammation and Prevents Autism-Related Behaviors in Offspring Caused by Maternal Immune Activation.

Autism spectrum disorder (ASD), a neurodevelopmental disorder of unknown etiology with limited treatment options, has emerged as a significant public health concern. Studies have demonstrated that prenatal vitamin D deficiency is a risk factor for ASD development in offspring; however, the underlying mechanism remains unclear. In this project, vitamin D was administered orally to pregnant mice with/without the subsequent administration of polyriboinosinic polyribocytidylic acid (Poly(I:C)), which induced the maternal immune activation (MIA).

Effectiveness of Restoration Strategies for Posterior Missing Teeth With Dental Implants: A Retrospective Study.

Aim: This study aimed to assess the effectiveness of a treatment strategy involving only implant restoration of the first molars (M1s) in cases where both M1s and second molars (M2s) lost.

Materials And Methods: A retrospective study design compared two groups: one underwent simultaneous implant restoration of both M1s and M2s (Group 1), while the other underwent restoration of only M1s (Group 2). Statistical models were used to evaluate implant survival, complication-free survival rates, cumulative treatment costs, peri-implant conditions, and patient-reported outcomes (PROMs).

Naringenin Attenuates Ox-LDL-induced Injury in HUVECs by Regulating Lipophagy Partly through the SOCE Pathway.

Objective: Naringenin (NAR) is a naturally occurring tiny molecule that has a significant role in lipid metabolism. However, the molecular mechanism by which NAR is involved in lipid metabolism to protect ECs is not clear. This study aims to investigate the effect of NAR on autophagy in oxidized low-density lipoprotein (ox-LDL)-treated human umbilical vein endothelial cells (HUVECs) and its potential molecular mechanisms.

Self-assembled TMB-CuO nanosheets for dual-mode colorimetric and NIR-II photothermal detection of uranyl ion.

Background: The development of highly sensitive and selective point-of-care testing (POCT) techniques using optical methods for uranyl ion (UO) is essential for the effective monitoring of acidic uranium contamination. colorimetric methods have gained significant attention for their rapid response times, ease of use, and cost-effectiveness in the quantitative analysis of uranyl ions.

Results: Herein, this study presents a self-assembled nanosheet composed of 3,3,5,5-tetramethylbenzidine (TMB) and CuO, designed for the dual-mode colorimetric and photothermal detection of UO.

AuCu@CuO Aerogels with HO/O Self-Supplying and Quadruple Enzyme-Like Activity for MRSA-Infected Diabetic Wound Management.

Diabetic wound healing presents serious clinical challenges due to the unique wound microenvironment characterized by hyperglycemia, bacterial infection, excessive oxidative stress, and hypoxia. Herein, a copper peroxide (CuO)-coated AuCu bimetallic aerogel is developed that exhibits quadruple enzyme-mimicking activity and HO/O self-supplying to modulate the complex microenvironment of methicillin-resistant staphylococcus aureus (MRSA)-infected diabetic wounds. The AuCu@CuO aerogels demonstrate favorable photothermal properties and mimic four enzyme-like activities: peroxidase-like activity for producing toxic reactive oxygen species; catalase-like activity for decomposing HO to release O to relieve oxidative stress and hypoxia; glucose oxidase-like activity for reducing excessive blood glucose and glutathione peroxidase-like activity for balancing abnormal glutathione level.

Mechanism Analysis of Amphotericin B Controlling Postharvest Gray Mold in Table Grapes.

Gray mold, caused by , is the primary factor contributing to postharvest losses in table grape fruit. In this study, we have identified amphotericin B (AMB), a natural compound originating from , as a promising agent in managing postharvest gray mold in table grapes. In vitro experiments demonstrated that 0.

Spatial regulation of chlorophyll degradation in kiwifruit: AcNAC2-AcSGR1/2 cascades mediate rapid de-greening in the inner pericarp.

Changes in skin colour, as a visual cue for fruit ripeness, are important physiological markers in many crops including tomato, banana and grape. In kiwifruit, the skin remains brown during ripening, but de-greening of the pericarp occurs to reveal accumulated carotenoids and anthocyanins in gold- and red-fleshed cultivars. In this study, analysis of the inner and outer pericarp of Actinidia chinensis 'Hongyang' revealed faster chlorophyll degradation in the inner pericarp, compared with the outer pericarp.