A patient with severe fever with thrombocytopenia syndrome complicated with intracranial and pulmonary aspergillosis.

Some patients with severe fever with thrombocytopenia syndrome (SFTS) develop complications including viral encephalitis and fungal infections, with a mortality rate reaching 44.7 %. In this case, the patient was admitted on Day 5 of illness, with symptoms of apathy and a delayed response.

Effects of Gender Differences in 677C > T and Homocysteine Level on the Occurrence of Adverse Pregnancy Outcomes.

The MTHFR 677C > T polymorphism in women has been associated with an increased risk of deep venous thrombosis and adverse pregnancy outcomes (APOs). However, research concerning its effects in men remains limited. This study examined 662 adults with a history of pregnancies affected by chromosomal abnormalities (CAs: 343 females and 319 males), 137 adults with a history of pregnancies affected by cleft lip and palate (CLP: 71 females and 66 males), and 133 adults with a history of biochemical pregnancies (BPs: 65 females and 68 males), forming three case groups.

One-Pot Isothermal Nucleic Acid Amplification Assisted CRISPR/Cas Detection Technology: Challenges, Strategies, and Perspectives.

The cutting-edge CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat)/Cas (CRISPR-associated proteins) system, as an emerging molecular diagnostic technique, is driving revolutionary developments in the detection field due to its high specificity and efficiency. However, the CRISPR-based assays typically require the combination with an additional pre-amplification step based on isothermal nucleic acid amplification to meet the requirements of clinical diagnosis, which brings issues including complicated operation and the risk of aerosol contamination. To address these challenges, one-pot CRISPR platforms are emerging as an attractive solution to streamline workflows, enabling rapid, cost-effective, and high-sensitivity diagnostics.

Auditory evoked potential wave VI as an objective indicator of sedation depth in neonates undergoing chloral hydrate sedation: a double-blind randomized controlled study.

Background: Neonatal sedation depth monitoring is critical yet depends on the subjective Ramsay scale when used and lacks objective biomarkers. Although auditory evoked potential (AEP) wave VI disappearance is linked to reduced consciousness, its use for neonatal sedation monitoring remains underexplored. We aimed to determine whether wave VI could function as an objective indicator of sedation levels in neonates.

Effects of Region, Processing, and Their Interaction on the Elemental Profiles of Pu-Erh Tea.

Elemental contents are effective fingerprints for Pu-erh tea's geographical traceability, which is crucial for consumer protection and sustainable development. Region and processing methods are key factors influencing the tea's elemental fingerprint. This study analyzed 28 elements in Pu-erh tea samples from three Yunnan production regions subjected to different processing stages in the year of 2023.

Discrimination of different varieties of rice in Wuchang area based on -nose and HS-SPME-GC-O-MS.

This study aimed to discriminate between different varieties of rice from the Wuchang region using a combination of electronic nose (-nose), headspace solid-phase microextraction-gas chromatography-olfactometry-mass spectrometry (HS-SPME-GC-O-MS), and chemometric analysis. Six rice varieties (Wuyoudao No. 4 (WC54), Longyang No.

Identification of Differential Metabolites in Chronic Suppurative Otitis Media With Non-Targeted and Targeted Metabolomics Approach.

Chronic suppurative otitis media (CSOM) is a leading cause of hearing loss and otorrhea, and when associated with cholesteatoma, it can pose a serious threat to patients' lives. This study aims to identify differences in tissue metabolites between patients with CSOM, both with and without cholesteatoma. Metabolomic profiles were measured in tissue samples from 42 surgically treated CSOM patients (35 with cholesteatoma, 7 without cholesteatoma).

Predictive value and ranking of writhing and fidgety movements for cerebral palsy: A meta-analysis based on the Superiority Index.

Background: This meta-analysis assessed the diagnostic value of writhing and fidgety general movements (GMs) for predicting cerebral palsy.

Methods: We searched PubMed, EMBASE, Cochrane Library, CINAHL, and Web of Science for cohort or case-control studies evaluating GMs. We calculated the superiority index (S), sensitivity, specificity, likelihood ratios, diagnostic odds ratio (DOR), and area under the curve (AUC) using STATA.

EAT-Lancet diet and risk of metabolic dysfunction-associated steatotic liver disease and other liver chronic diseases: a large prospective cohort study in the UK Biobank.

Background And Aim: As a newly recommended healthy dietary blueprint, the EAT-Lancet diet emphasizes both environmental sustainability and human health. However, its impact on chronic liver diseases remains unclear. This study examined the influence of the EAT-Lancet diet on the risk of metabolic dysfunction-associated steatotic liver disease (MASLD) and other chronic liver diseases.

Impact of Genetic Ancestry on Genomics and Survival Outcomes in T-cell Acute Lymphoblastic Leukemia.

Unlabelled: The influence of genetic ancestry on genomics in T-cell acute lymphoblastic leukemia (T-ALL) has not been fully explored. We examined the impact of genetic ancestry on multiomic alterations, survival outcomes, and risk stratification. Among 1,309 children and young adults with T-ALL treated on the Children's Oncology Group trial AALL0434, the prognostic value of five commonly altered T-ALL genes varied by ancestry-including NOTCH1, which was associated with superior overall survival for patients of European ancestry but was nonprognostic among patients of African ancestry.

Correlation of serum miR-203a-3p with the severity of neonates with necrotizing enterocolitis and analysis of its molecular mechanism.

Objectives: The objective of this study was to examine the role of microRNA-203a-3p (miR-203a-3p) in the pathogenesis of necrotizing enterocolitis (NEC).

Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) and receiver operating characteristic (ROC) curve analysis were employed to evaluate the relative abundances of miR-203a-3p as well as its diagnostic capacity. Logistic regression was applied to search for influential risk factors associated with NEC in neonates.

Insights into the molecular response mechanisms of fasting stress and refeeding in channel catfish (Ictalurus punctatus) through transcriptome and histological analysis.

Throughout their life cycles, many fish alternate between periods of fasting and feeding due to the temporal and geographical variability of food availability in aquatic environments. The ability to adapt to fasting and restore internal balance after refeeding is fundamental to the long-term survival and symbiotic relationships of aquatic species. However, our understanding of the mechanisms by which the fish adapt to fasting and refeeding remains limited.

Association of the triglyceride-glucose index with risk of depression and anxiety: A prospective cohort study.

Background: The relationship between the triglyceride glucose (TyG) index and depression/anxiety remains unclear. This study prospectively examines whether elevated TyG index increases depression and anxiety risk.

Methods: Our analysis included 383,621 individuals from the UK Biobank who were free of depressive and anxiety disorders at enrollment.

Sea buckthorn flavonoids and their derivatives: potential natural compounds for the treatment of diabetic cardiomyopathy.

Diabetic cardiomyopathy (DCM) is one of the most common complications of diabetes, characterized by high morbidity and disability rates, and can lead to heart failure. However, specific therapeutic agents for DCM are currently lacking. Natural compounds derived from traditional Chinese medicine have demonstrated potential in alleviating DCM through multiple mechanisms.

Pancreatic extracellular volume fraction based on dual-energy computed tomography iodine maps: association with diabetes.

Background: Pancreatic fibrosis is closely related to blood glucose levels in diabetes, and pancreatic extracellular volume fraction (fECV) can quantify pancreatic fibrosis. The aim of this study was to investigate the correlation between pancreatic fECV measured by dual-energy computed tomography (DECT) iodine maps and hemoglobin A1c (HbA1c), and the feasibility of fECV in evaluating the progression of diabetes.

Methods: This retrospective cross-sectional study included 213 patients who underwent abdominal contrast-enhanced DECT examinations; they were divided into two groups: non-diabetes (Group ND) and diabetes (Group D).

Molecular Characterization and Feeding-Associated Expression Dynamics of the Period Gene Family in Channel Catfish ().

Rhythms, regulated by core clock genes like the period () gene family, are crucial for maintaining physiological processes in animals. In teleost fish, including channel catfish (), these genes have evolved distinct functions. However, the evolutionary characteristics and functional roles of period genes, particularly in response to environmental cues such as feeding, remain unclear.

Drug loaded hydrogel nanoparticles with long retention in tumor and multi drug co delivery for melanoma treatment.

This study aims to address the issue of immune clearance and inaccurate targeting of drug-loaded nanoparticles during intravenous injection, which may lead to partial drug release into non target tissues or organs, damage to normal tissues and low drug utilization. Herein, multi-drug co-delivery nanoparticles with intratumoral long retention capability were developed for melanoma treatment. This study adopted a dual drug loading strategy of curcumin and dexamethasone and designed a drug-loaded nano-system based on polyglutamic acid and caffeic acid.

Early Outcomes of Chimney Commando Procedure In Redo Double Valve Replacement: A Retrospective Cohort Study.

Objective: To assess the early outcomes of the Chimney Commando (CC) procedure in patients who underwent redo double valve replacement (DVR) with and without CC.

Methods: A retrospective cohort study of 194 patients undergoing redo double valve replacement (DVR) at Wuhan Asia Heart Hospital from December 2019 to December 2024. 101 patients were in CC+DVR group and 93 in isolated DVR group.

Highly efficient photoelectrochemical biosensor for the evaluation of bisphenol A-induced microRNA-21 expression alterations.

Herein, we developed a photoelectrochemical (PEC) biosensor integrating SrTiO and SnO materials with a dumbbell DNA mediated target recycling system to quantify microRNA-21 levels under bisphenol A (BPA) exposure. The engineered SrTiO/SnO photoelectrode demonstrated enhanced charge separation efficiency due to band alignment, yielding a remarkable 33-fold photocurrent enhancement compared to that of pure SrTiO. In addition, a dumbbell DNA-based signal amplification strategy was designed and the target microRNA-21 was converted to DNA nanospheres, which can "switch-on" the PEC signal by loading the signal mediator methylene blue.

Comparative analysis of pectic polysaccharides isolated from various thinned young red-fleshed kiwifruits: Structural properties and biological functions.

To utilize thinned young kiwifruit for potential industrial applications, this study compared structural properties and bioactivities of pectic polysaccharides isolated from three thinned young red-fleshed kiwifruit cultivars, including cv. 'Donghong', 'Hongshi', and 'Hongyang'. The results demonstrated that these young kiwifruit byproducts were rich in pectic polysaccharides, with extraction yields of 90.

Association between obesity indexes and chronic kidney disease risk: a double-cohort prospective study in the Binhai and UK Biobank.

Introduction: The prevalence of overweight and obesity has increased worldwide, leading to growing concern regarding the impact of visceral adipose deposition on renal function. The aim of this study was to evaluate the predictive value of 10 obesity indexes for the risk of chronic kidney disease (CKD) in both Chinese populations and Western.

Methods: The Tianjin Chronic Kidney Disease Study (Binhai, primary cohort) included 126,109 participants, while 358,918 adults from the U.

Topography-driven variability in atmospheric deposition and soil distribution of cadmium, lead and zinc in a mountainous agricultural area.

Topography can significantly influence the atmospheric deposition of heavy metals on the surface soil and further shape their spatial distribution. This study was carried out in Northwestern Guizhou Province, a key agricultural region contaminated with cadmium (Cd), lead (Pb), and zinc (Zn) in agricultural soil. In this study, geographical detectors were employed to quantitatively assess the impact of topography on the spatial distribution of Cd, Pb, and Zn in surface soils.

BVDV non-structural protein 4B promoted autophagy by regulating p62/LC3-II molecule.

Bovine viral diarrhea virus (BVDV) causes bovine viral diarrhea-mucosal disease that is characterized by diarrhea, fever, leukopenia, and propagation disorders in host animals. Autophagy acts an important regulatory role during virial infection. However, the molecular mechanisms that BVDV induces autophagy remain poorly defined.

Gold-catalyzed azidation, cyanation, and alkynylation of aryl iodides from readily available TMS-N, TMS-CN, and alkynyl-TMS.

We have developed a unified protocol for gold-catalyzed azidation, cyanation, and alkynylation of aryl iodides from readily available silane-based nucleophiles (Nu-TMS) such as TMS-N, TMS-CN, and alkynyl-TMS. The key step is the activation of Nu-TMS by a suitable silver activator to generate reactive Ag-Nu intermediates. The reaction is based on readily available starting materials (eliminating the need for toxic reagents such as NaN and KCN), a broad substrate scope, and straightforward conditions (no requirement for air- or water-free conditions).