Functional divergence of G protein γ subunits drives plant vigor and improvement through the CPK28-PIP1;2 pathway in tomato.

Maintaining robust plant vigor is essential for sustaining crop productivity, yet the precise roles and molecular underpinnings of G protein γ subunits in this process remain elusive. This study reveals that GGC1 is under selection during tomato domestication, and its mutants exhibit enhanced plant vigor, characterized by superior growth, increased yield, and improved fruit quality. In contrast, triple mutants gga1/ggb1/ggb2 display severely compromised vigor resembling slgb1 mutants lacking the Gβ subunit.

Medicaid Primary Care Utilization and Area-Level Social Vulnerability.

Importance: The concentration of poverty and multidimensional disadvantage has been shown to limit access to health care in these communities. There is a growing interest in using area-level socioeconomic indexes to address the unequal geographic distribution of health care resources. However, the association of area-level socioeconomic indexes with access to primary care-a key area in health policy-has not been determined.

Do Primary Care Providers' Medicaid Panels Represent the Communities They Serve?

Disparities in primary care access among Medicaid enrollees may be driven by differences in provider acceptance of Medicaid, yet the extent to which primary care provider (PCP) panels reflect the racial and ethnic diversity of local Medicaid populations is unknown. : To quantify the alignment between the racial/ethnic composition of PCP Medicaid panels and the underlying Medicaid population in their service areas. : We conducted a cross-sectional analysis of 2019 Transformed Medicaid Statistical Information System Analytic Files from 44 states focusing on non-Hispanic White (NHW), non-Hispanic Black (NHB), and Hispanic enrollees.

Identifying Opponent's Neuroticism Based on Behavior in Wargame.

Traditional neuroticism assessments primarily rely on self-report questionnaires, which can be difficult to implement in highly confrontational scenarios and are susceptible to subjective biases. To overcome these limitations, this study develops a machine learning-based approach using behavioral data to predict an opponent's neuroticism in competitive environments. We analyzed behavioral records from 167 participants on the MiaoSuan Wargame platform.

Synergistic chemotherapy and phototherapy co-delivery nanoparticle preparation and anti-triple negative breast cancer study.

In this study, the amphiphilic polymer HA-ANI, formed by grafting hyaluronic acid (HA) and 6-(2-nitroimidazolyl)hexylamine (ANI), was self-assembled in water to form nanoparticles (NPs), which were modified to obtain dual drug-loaded nanoparticles (P/I NPs) by dialysis loading of paclitaxel (PTX) and IR780. Electron microscopy and dynamic light scattering (DLS) results showed that the P/I NPs presented a near-spherical shape with a size of 221.10 ± 2.

METTL16 Promotes Lipid Metabolic Reprogramming and Colorectal Cancer Progression.

Lipid reprogramming represents a pivotal stage in tumor progression. N6-methyladenosine (m6A), the most prevalent RNA modification in eukaryotic cells, plays a significant role in colorectal cancer (CRC) development, though its specific involvement in lipid reprogramming remains unclear. Bioinformatics analysis of The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases revealed differential expression of METTL16 (M16), which was further validated through qRT-PCR and Western blotting in CRC tissues and cell lines.

Effects of total urethral suspension combined with posterior pelvic floor reconstruction on sexual function in women with stress urinary incontinence and concomitant vaginal laxity syndrome.

Objective: To assess the impact of total urethral suspension with posterior pelvic floor reconstruction on sexual function in women with stress urinary incontinence(SUI) and concomitant vaginal laxity syndrome (VLS), including partner satisfaction.

Methods: Clinical data from 150 pelvic floor dysfunction patients were collected at the First Affiliated Hospital of Kunming Medical University (March 2023-March 2024). Preoperative assessments included demographics, obstetric/surgical history, menopausal status, sexual activity and .

Evaluating the therapeutic effect of Moutan cortex-Gardeniae Fructus-Coptidis Rhizoma on C57BL/6 mice with diabetic cardiomyopathy based on HPLC-network pharmacology and in vivo animal experiments.

DCM is a serious complication of diabetes that eventually develops into heart failure and damages the health of human. Here, we study the therapeutic effect of MC-GF-CR on C57BL/6 mice with DCM. First, the aqueous extract powder of MC-GF-CR was characterized by HPLC for compositional analysis.

Asymmetric hand deformities with limited mobility.

A 52-year-old woman presented to the rheumatology department with a 6-year history of progressively limited hand mobility, bilateral joint pain, and worsening hand deformities. Examination revealed asymmetric deformities of both hands, more pronounced on the left (Fig, A), without joint tenderness, swelling, or Heberden's and Bouchard's nodes. Laboratory tests, including C-reactive protein, rheumatoid factor, anticyclic citrullinated peptide antibody, antinuclear antibody, antidouble-stranded DNA antibody, and anti-Smith antibody, revealed no abnormalities.

Preparation and functional characterization of buckwheat protein glycosylation products.

Buckwheat protein (BP) exhibits a nutritionally balanced amino acid profile and demonstrates multiple bioactive properties, although certain functional limitations restrict its broader application. This study systematically optimized the reaction conditions for both wet/dry-heat glycosylation between BP and polysaccharide (FEP), characterized the effects of these conjugation methods on BP's functional properties, and successfully employed the resulting bioconjugates for vitamin D₃ (VD) encapsulation. The data demonstrate that the wet glycosylation method achieved a degree of grafting (DG) of 17.

Challenges in Thirst Management for Intubated Patients in the ICU: A Qualitative Study of Healthcare Provider and Patient Perspectives.

Objective: This study aims to examine the challenges associated with thirst management among patients undergoing tracheal intubation in intensive care units (ICUs), and to inform the development of more effective clinical strategies.

Methods: A qualitative research design was employed using semi-structured interviews with 32 participants, including healthcare professionals and patients who had experienced tracheal intubation and associated thirst in the ICU. Interview content addressed themes such as the expression, recognition, and management of thirst.

Association between uric acid to high-density lipoprotein cholesterol ratio and moderate-to-severe perivascular spaces burden: a retrospective cross-sectional study.

Background: Perivascular spaces (PVS) are critical for waste clearance in the central nervous system and are implicated in various neurological disorders. The uric acid to high-density lipoprotein cholesterol ratio (UHR) is a novel inflammatory-metabolic marker, but its association with PVS burden remains unexplored. This study investigated the association between UHR and moderate-to-severe PVS burden.

Enumerating the Oncology Specialist Workforce in Medicaid: Applying a Triangulated Approach.

Objective: To develop a novel method for enumerating the oncology specialist workforce triangulating taxonomy codes, board certification data, and clinical diagnosis codes in Medicaid claims, and to describe oncology specialists' Medicaid participation, their patient panels, and ascertain the concentration of types of cancers they treated.

Study Setting And Design: We identified oncology specialists using multiple data sources and conducted an exploratory analysis of their patient panels using multi-state Medicaid claims data. We used cluster analysis of diagnosis code patterns in claims to accurately determine the concentration of cancers by site in oncologists' panels.

Multifunctional N-doped carbon dot- polyvinyl alcohol composite film/label enables real-time freshness monitoring and synergistic preservation for shrimp.

Conventional food packaging lacks active preservation and real-time monitoring. This study presents a novel polyvinyl alcohol -based film/label incorporating N-doped carbon dot, exhibiting enhanced pH responsiveness, potent antimicrobial/ antioxidant properties and biosafety (cell relative viability >90 %). The pH-sensitive label can capture the volatile amines from food spoilage, triggering its real-time dual-mode colorimetric/fluorescent signal changes with trimethylamine detection limit of 0.

Workforce Serving Pregnant and Postpartum Medicaid Enrollees at Community Health Centers, 2016 to 2021.

Introduction: Community Health Centers (CHCs) care for nearly a third of all pregnant Medicaid enrollees. Given that Medicaid covers 41% of childbirths, CHCs play a critical role in ensuring pregnant enrollees' access to perinatal services. Despite their importance, little is known about the CHC workforce serving these patients.

Engineering Xylose Metabolism and Coutilization with Glucose in for Efficient Microbial Cell Factories.

Xylose, an important sugar component of lignocellulosic biomass, is often inefficiently utilized due to limitations in its transport and metabolic pathways. This study aimed to enhance the xylose metabolism in through metabolic engineering and transcriptional regulation strategies. First, the xylose assimilation pathway was constructed by the heterologous expression of xylose isomerase (XylA) and xylulose kinase (XylB), which improved the strain's xylose metabolic capability.

An Olsalazine-Loaded Hollow Mesoporous Prussian Blue/Polydopamine Nanozyme Alleviates Anxiety/Depression-like Behavior and Enhances Brain Neural Activity in Colitis Mice.

Individuals diagnosed with inflammatory bowel disease (IBD) exhibit a markedly elevated prevalence of psychiatric disorders, particularly anxiety and depression. However, the underlying mechanisms contributing to the development of these psychiatric conditions in IBD patients remain poorly understood, and no effective therapeutic strategy has been definitively established. Recent advancements in nanotechnology-based interventions offer promising potential for restoring gut homeostasis, which may mitigate inflammation and oxidative stress associated with IBD.

Oral olsalazine/ellagic acid/ZnCl-modified in situ-forming hydrogels alleviates anxiety/depression-like symptoms and brain neural activity in colitis mice.

Inflammatory bowel disease (IBD) is a kind of chronic non-specific intestinal inflammatory disease, which accompanied by various abnormal psychological states, such as anxiety and depression. Nevertheless, the pathogenesis of IBD with depression is still unclear. Herein, we synthesized an enable a drinkable, liquid in situ-forming hydrogel, which form directly in the stomach through sequential ingestion of a crosslinker solution of CaCl and ε-poly-l-lysine (ε-PL), followed by a drug-containing polymer solution of sodium alginate (SA).

Sishen pill alleviates ulcerative colitis via the NLRP3/ASC/Caspase-1 signaling pathway: Comprehensive validation through UPLC-Q-TOF/MS, network pharmacology, molecular docking, and in vivo experiments.

Background: The "Sishen Pill" (SSP) is a traditional Chinese medicinal formulation traditionally employed in the treatment of diarrhea attributed to spleen-kidney yang deficiency, and it has exhibited notable clinical efficacy in managing ulcerative colitis (UC). Nevertheless, the bioactive compounds and the underlying mechanisms by which SSP exerts its therapeutic effects on UC remain inadequately elucidated.

Objectives: This study sought to systematically elucidate the bioactive constituents and the mechanism of action of SSP in the treatment of UC through the application of ultra-high-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS), network pharmacology, molecular docking, and animal experimentation.

Structural connectivity of hippocampus is not altered in patients with type 2 diabetes and without peripheral microangiopathy.

Functional hyper-connectivity in hippocampus has been recently observed in patients with an early stage of type 2 diabetes, however, the structural connectivity between hippocampus and other regions of the brain and its effects on neuropsychological performance are still unknown. Participants (82 patients with type 2 diabetes and without peripheral microvascular complications; 75 healthy controls) underwent detailed cognitive assessment and diffusion MRI. Probabilistic tractography based on a multifiber model was performed to investigate the fiber connections between hippocampus and other regions of the brain.

Association between cerebral small vessel disease and malnutrition risk: a retrospective cross-sectional study.

Introduction: Malnutrition is associated with increased morbidity and mortality from multiple diseases. However, the relationship between cerebral small vessel disease (CSVD) and malnutrition or malnutrition risk remains underexplored. This retrospective study investigated the association between malnutrition risk and CSVD, along with its common imaging markers.

BRD4 acts as a transcriptional repressor of RhoB to inhibit terminal erythropoiesis.

Background: Terminal erythropoiesis is a complex multistep process involving coordination of gene transcription and dramatic nuclear condensation, which leads to the expulsion of nuclei to generate reticulocytes. However, we lack a comprehensive understanding of the key transcriptional and epigenetic regulators involved.

Methods: We used a high-throughput small molecule screen in primary CD34-derived human erythroblasts to identify targets that promoted terminal erythropoiesis, and further confirmed the phenotype in different differentiation systems by inhibitors and shRNAs of different BRD4 isoforms.

Age-dependent effects of HS on post-traumatic stress disorder in adolescent and adult mice.

Background: Among people with post-traumatic stress disorder (PTSD), there is an increased prevalence of age-related diseases. However, the biological mechanisms underlying this phenomenon remain incompletely understood.

Methods: The expression of cystathionine β-synthase (CBS), one of the main enzymes for endogenous hydrogen sulfide (HS) production in the brain, is age-dependent.

The Association of Blood Cobalt Levels with Cardiovascular and Chronic Kidney Diseases: Mediating Role of Inflammatory Indicators (SII, NLR, PLR, NMLR, and LMR).

Cobalt is a prevalent environmental metal with known toxicological potential. Inflammation plays a key role in the pathophysiology of cardiovascular disease (CVD) and chronic kidney disease (CKD). However, the relationships between blood cobalt concentrations, inflammatory indicators, and their roles in CVD and CKD remain inadequately characterized.

Early cardiac involvement in patients with hyperthyroidism evaluated by cardiac magnetic resonance feature tracking.

Background: This study aimed to investigate the feasibility of quantitative assessment of myocardial injury in the early stages of the disease by myocardial strain in patients with hyperthyroidism.

Methods And Results: A total of 45 individuals in the hyperthyroid state with at least one index of cardiac involvement (e.g.