Transcription Factor GmERFA Interacts With GmNFYA and Acts as a Negative Regulator of Seed Fatty Acid Accumulation in Soybean.

Soybean is one of the most important oilseed crops, and its seed oil content directly determines the economic value and industrial applicability worldwide. However, how soybean seed oil accumulation is regulated remains less understood. Here, through RNA-seq analysis and screening for the interacting proteins of a positive oil regulator GmNFYA, we identified an AP2/ERF-type transcription factor GmERFA, which acts as a negative regulator of oil accumulation.

Activation of Locus Coeruleus-Hippocampus Tyrosine Hydroxylase Projection Contributes to the Surgical Incision Pain-Induced Memory Consolidation Enhancement in Mice.

Aims: The mechanism underlying postoperative post-traumatic stress disorder (PTSD) remains unclear. However, studies have shown that acute postoperative pain is an independent risk factor for PTSD, which is also closely related to memory consolidation enhancement. Preoperative patients often experience unpleasant traumatic events, and postoperative pain usually occurs in the memory consolidation stage of these events.

Coordinated regulation of pH alkalinization by two transcription factors promotes fungal commensalism and pathogenicity.

As a clinically relevant opportunistic human fungal pathogen, Candida albicans is able to rapidly sense and adapt to changing microenvironments within the host, a process that is essential for its successful invasion and survival. Although studies have shown that the transcription factor Stp2 is the master regulator of environmental alkalinization, accumulating evidence supports a clear involvement of other participants in this adaptation process. Here, following a large-scale genetic screen, we identify the transcription factor Dal81 as an uncharacterized positive regulator of pH alkalinization in C.

The miR172a-ERF416/413 module regulates soybean seed traits.

Soybean (Glycine max) provides vegetable oils and proteins for human consumption. Its production depends on seeds and other production-related agronomic traits. How the seed traits are regulated in soybean remains largely unclear.

Reversing the "cold" tumor microenvironment: the role of neoantigen vaccines in prostate cancer.

Background: Prostate cancer (PCa) is a common malignancy with a "cold" tumor microenvironment (TME) that limits immunotherapy efficacy. Neoantigens from non-synonymous mutations are potential immunotherapeutic targets.

Methods: We identified the neoantigens map in the RM-1 murine prostate cancer model by the whole exome sequencing.

Assessment of long-term survival in childhood cancer patients using cancer registry data from eastern China: Period analysis outperforms traditional analysis.

This study systematically evaluated whether period analysis outperforms traditional cohort and complete analyses in estimating 5-year relative survival (RS) for childhood cancer patients using data from nine cancer registries in Taizhou, Eastern China (2009-2018). Analyses included patients under 15 years diagnosed with childhood cancers, with 5-year RS estimates from cohort, complete, and period methods compared against observed actual survival (2014-2018: 70.1%).

Machine learning-based prediction of preterm birth risk using methylation changes in neonatal cord blood CpG sites.

Background: Preterm birth, defined as delivery before 37 weeks of gestation, is a major cause of neonatal morbidity and mortality. DNA methylation changes at CpG sites have been associated with the risk of preterm birth.

Objective: This study aimed to identify differential CpG sites in cord blood and develop predictive machine learning models based on these methylation changes to assess preterm birth risk.

Oral olsalazine/ellagic acid/ZnCl-modified in situ-forming hydrogels alleviates anxiety/depression-like symptoms and brain neural activity in colitis mice.

Inflammatory bowel disease (IBD) is a kind of chronic non-specific intestinal inflammatory disease, which accompanied by various abnormal psychological states, such as anxiety and depression. Nevertheless, the pathogenesis of IBD with depression is still unclear. Herein, we synthesized an enable a drinkable, liquid in situ-forming hydrogel, which form directly in the stomach through sequential ingestion of a crosslinker solution of CaCl and ε-poly-l-lysine (ε-PL), followed by a drug-containing polymer solution of sodium alginate (SA).

Three-Dimensional-Printed Gelatin Methacryloyl Scaffold Loaded with Extracellular Vesicles Derived from HS Preconditioned Mesenchymal Stromal Cells Promotes Neuronal Regeneration in Rats with Spinal Cord Injury.

Background: spinal cord injury (SCI) causes irreversible motor and sensory deficits with limited effective treatments. Mesenchymal stromal cells (MSCs) exert therapeutic effects largely through extracellular vesicles (EVs). Preconditioning MSCs with a hydrogen sulfide (HS) donor enhance the therapeutic potential of EVs.

Single-cell sequencing and mass spectrometry imaging reveal the multicellular compartmentalization map of plant triterpenes: A ginsenoside in Panax ginseng example.

Revealing the multicellular compartmentalization map is crucial for plant breeding and metabolism regulation. Despite the importance of triterpenes as active compounds, there has been no report on their biosynthetic multicellular mapping. In this study, ginsenosides are used as an example to reveal the multicellular compartmentalization map of plant triterpenes.

Efficacy and safety of anti-PD-1 antibodies plus small molecule anti-angiogenic drugs and chemotherapy in gastric cancer peritoneal metastasis: a multicenter real-world study.

Background: Immunotherapy combined with chemotherapy has emerged as the first-line standard treatment for advanced gastric cancer. However, obvious survival benefits in patients with peritoneal metastatic gastric cancer remain elusive. The combination of anti-angiogenic agents and immunotherapy has shown synergistic effects.

Nebulization with differently sized 2% lidocaine atomized particles in awake tracheal intubation by video laryngoscopy: a proof-of-concept randomized trial.

Background: Optimization of the individual topicalization technique during awake tracheal intubation (ATI) is critical, but how to accomplish this is still unknown. We hypothesized that there would be a difference between nebulization with different sizes of 2% lidocaine atomized particles in ATI by video laryngoscopy.

Methods: A total of 230 patients scheduled for ATI were recruited from September 2024 through January 2025.

Human capital, technological progress and industrial restructuring.

Human capital and technological progress drive industrial restructuring toward rationalization and advancement, clarifying their theoretical interaction and examining the influence mechanism based on China's three major industries data from 2008 to 2022, employing the PVAR and Hansen threshold models. The study shows that: (1) technological progress and industrial restructuring are mutually causal, while technological progress and human capital, and human capital and industrial restructuring are not mutually causal. (2) Within the research cycle, human capital has a 'fast then slow then smooth' influence on industrial structural adjustment, while technological progress has a 'V' influence on industrial structural adjustment, and technological progress has a 'U' influence on human capital.

The transcription factor LpWRKY65 enhances embryogenic capacity through reactive oxygen species scavenging during somatic embryogenesis of larch.

Somatic embryogenesis is a powerful system for studying embryo development and scaling up the production of elite genetic material. Somatic embryogenesis has been well established in Larix principis-rupprechtii, a Chinese larch species dominant in the world's largest man-made forest. However, genotype-dependent embryogenic variations hinder large-scale forestry, and the molecular mechanisms remain unclear.

Patient-derived organoids inform pharmacogenomic vulnerabilities in liver cancer.

Background & Aims: Patient-derived tumor organoids (PDTOs) are a reliable model for preclinical and translational studies. Despite positive retrospective correlations with patient response, challenges such as culture success, cost, standardization, and time constraints hinder their clinical utility in precision medicine. Here, we optimize PDTO establishment using growth factor-reduced media (GF-) to mitigate these challenges and (1) identify somatic variant indicators that can improve the therapeutic index of existing FDA-approved drugs against hepatocellular carcinoma (HCC), (2) elucidate synthetic lethal candidates against undruggable HCC driver mutations, and (3) assess the feasibility of PDTOs in personalized therapy.

Single- and Multi-Locus GWAS Unravels Novel Genomic Regions Related to Low-Phosphate Stress in Cotton Seedlings.

Phosphorus (P) is an essential nutrient for plant growth, and low-phosphorus (LP) stress significantly limits cotton productivity. Here, we conducted single- and multi-locus genome-wide association studies (GWASs) on four LP-related traits using 419 upland cotton ( L.) accessions genotyped with 2.

Homeobox C6 is Up-Regulated and Affects the Pathogenesis of Endometriosis.

Endometriosis is one of the most common, difficult, and complicated gynecological disorders. The present study investigated the expression of HOXC6 in endometriosis and described its possible role in its pathogenesis. Ectopic and eutopic endometrial samples from 21 patients with endometriosis and a control endometrium from 15 women without endometriosis were collected.

Enhanced removal of tetracycline and doxycycline in aquaculture pond sediments via synthetic bacterial consortium: Efficacy and ecological risk assessment.

Tetracycline antibiotics (TCs), extensively used in aquaculture and livestock production, pose significant ecological risks due to their accumulation in aquatic sediments. This study investigated the efficacy and ecological implications of bioaugmentation with a synthetic microbial consortium for the remediation of TCs-contaminated aquaculture pond sediment. Sediment microcosms were amended with tetracycline (TC) and doxycycline (DOX) at different dosages, with or without consortium inoculation.

Fine structural design of 3βHSD1 inhibitors for prostate cancer therapy.

Prostate cancer is a global health challenge, particularly for patients resistant to the second-generation anti-androgen receptor pathway inhibitors. The steroidogenic enzyme 3β-hydroxysteroid dehydrogenase type 1 (3βHSD1) has emerged as a promising therapeutic target and the corresponding inhibitors, biochanin-A (BCA) and its derivatives, suppress tumor growth in preclinical models and patients. However, the poor oral bioavailability of BCA hinders its clinical application.

Characterization of a small non-coding RNA S612 in .

Small regulatory RNAs (sRNAs) are non-coding RNA molecules that fine-tune various cellular processes and respond to various environmental stimuli. In , the regulatory mechanisms and specific targets of several sRNAs remain largely unknown. In this study, we identified and characterized S612 as a self-terminating sRNA in .

Dynamic changes in intestinal microbiota mediate mechanical hyperalgesia in surgical menopause model: a potential mechanism of DRG neuroinflammation.

The role of the intestinal microbiota in hyperalgesia in ovariectomized mice remains unclear. This study aimed to investigate pain behavior and dynamic changes in the intestinal microbiota and the levels of related metabolites in a model of surgical menopause and to verify the hypothesis that the intestinal microbiota mediates the occurrence and persistence of hyperalgesia through neuroinflammation. An ovariectomy (OVX) model was constructed to assess the intestinal microbiota composition, the levels of related metabolites, and inflammation levels in the spinal dorsal root ganglion (DRG).

Sex Hormone Levels and Pain Thresholds in the Luteal Phase of Healthy Women.

Objective: To explore the relationship between the level of endogenous sex hormones in the luteal phase and the pain threshold of healthy subjects, and to find the influencing factors of the change in female pain threshold.

Methods: Sixty-eight unmarried and nulliparous women, aged 22-28 years old, had regular menstruation with a cycle of 26-30 days, did not smoke, had no pain, no drug abuse, no gynecological problems, and had not participated in other clinical trials in the past 3 months. Blood was collected for analysis of sex hormone concentrations including follicle-stimulating hormone (FSH), luteinizing hormone (LH), and four different pain thresholds were measured after abdominal ultrasound confirmed that the menstrual cycle was in the luteal phase.

Artificial intelligence-driven internet of things-based green supply chain for carbon reduction in sustainable manufacturing.

Recent advancements in sustainable business practices and information technology trends have led to the rise of green smart supply chains. These green sustainable supply chains are a novel approach that involves information technology to enhance the operational standards throughout various industries. The integration of artificial intelligence and the Internet of Things has transformed these sectors by utilizing intelligent supply chain practices, improving efficiency and significantly reducing environmental impacts.

B7-H3 and c-MET in advanced prostate cancer: exploring possibilities of novel bi-specific drug development.

Purpose: Prostate cancer (PCa) is a serious malignancy worldwide with the fifth highest mortality rate among tumors in males, and there are unmet clinical needs for PCa treatment. In this work, we aim to explore the expression and overlap between B7 Homolog 3 protein (B7-H3) and cell-surface receptor c-mesenchymal-epithelial transition factor (c-MET), to provide insight on B7-H3 and c-MET bi-specific ADC drugs development for advanced PCa.

Patients And Methods: In this retrospective cross-sectional study, formalin-fixed paraffin-embedded (FFPE) samples were analyzed from 135 male patients with advanced PCa at Hubei Cancer Hospital between 2019 and 2023.

Effects of enzymes originating from on the performance, organ index, serum biochemical indices, and intestinal health homeostasis of broiler chicken.

To study the effect of combined enzymes from on growth performance, organ index, serum biochemical indices, and gut microbiota in broiler chickens, an experiment was conducted with 648 one-day-old AA broilers. The broilers were randomly divided into six groups. The control group was fed a corn-soybean meal basal diet, and the experimental groups were supplemented with different enzyme combinations.