Coordinated regulation of pH alkalinization by two transcription factors promotes fungal commensalism and pathogenicity.

As a clinically relevant opportunistic human fungal pathogen, Candida albicans is able to rapidly sense and adapt to changing microenvironments within the host, a process that is essential for its successful invasion and survival. Although studies have shown that the transcription factor Stp2 is the master regulator of environmental alkalinization, accumulating evidence supports a clear involvement of other participants in this adaptation process. Here, following a large-scale genetic screen, we identify the transcription factor Dal81 as an uncharacterized positive regulator of pH alkalinization in C.

Photocatalytic Aminative Suzuki-Miyaura C(sp)-N-C(sp) Cross-Coupling.

This study introduces a photoinduced Suzuki-Miyaura C(sp)-C(sp) coupling protocol in which the introduction of a nitrene reagent efficiently redirects the conventional C(sp)-C(sp) linkage toward a C(sp)-N-C(sp)-bridged architecture. A total of 432 reactions were conducted using a high-throughput screening strategy to identify the optimal parameters. This strategy exhibits mild conditions, convenient operation, and good functional group tolerance, meanwhile establishing a solid foundation for further developing novel catalytic systems of cross-coupling reactions.

A retrospective analysis of hemodynamic stability in TAVR and TTVR under general anesthesia: implications for sedation in TTVR.

Background: Transcatheter aortic valve replacement (TAVR) is routinely performed under general anesthesia (GA) or sedation. However, there were few studies focused on the anesthesia method when transcatheter tricuspid valve replacement (TTVR) was performed. This study aimed to investigate whether TTVR procedures can be performed effectively under sedation.

Dehydrotumulosic acid: a potential glucocorticoid receptor agonist with anti-coxsackievirus activity.

Background: Coxsackievirus infections cause a range of diseases, from hand-foot-and-mouth disease to severe conditions such as myocarditis and meningoencephalitis. High mutation rates and serotype diversity complicate clinical management, as no approved vaccines or therapies exist. Triterpenoids, natural compounds with antiviral and immunomodulatory effects, offer potential as novel treatments.

Cardiovascular safety of propranolol oral solution in infantile hemangiomas: a disproportionality analysis based on the FAERS database.

This study aims to explore the potential association between propranolol and cardiovascular adverse events (AEs) through real-world evidence. Reports from the FDA Adverse Event Reporting System (FAERS) database spanning from January 2014 to September 2024, identifying propranolol oral solution as the primary suspected (PS) drug, were analyzed. Disproportionality analysis employed four key metrics: Reporting Odds Ratio (ROR), Proportional Reporting Ratio (PRR), Bayesian Confidence Propagation Neural Network (BCPNN), and Multi-item Gamma-Poisson Shrinkage (MGPS) to identify positive signals of cardiovascular AEs potentially associated with propranolol oral solution.

Emission Characteristics of Evaporative Intermediate-Volatility Organic Compounds from In-Use Gasoline Vehicles.

The evaporation of intermediate-volatility organic compounds (IVOCs) from vehicle emissions substantially contributes to secondary organic aerosol (SOA) and ozone (O) formation. However, the limited characterization of evaporative IVOCs from in-use vehicles leads to uncertainties in assessing their atmospheric impact. Herein, aided by two-dimensional gas chromatography-mass spectrometry and flame ionization detectors (2D-GC-MS/FID), the highly resolved composition of IVOCs from in-use light-duty gasoline vehicles (LDGVs) are comprehensively characterized, revealing that the proportion of unresolved complex mixtures (UCMs) significantly decreased to 2.

Adaptive morphological changes link to poor clinical outcomes by conferring echinocandin tolerance in Candida tropicalis.

Antibiotic tolerance, by which susceptible bacteria survive at high bactericide doses, is known to cause treatment failure in clinical practice. However, the impact of antifungal tolerance on clinical outcomes remains poorly understood. Here, we observed that candidemia cases caused by echinocandin-tolerant Candida tropicalis exhibited higher mortality rates during caspofungin treatment by conducting a comprehensive seven-year retrospective analysis.

Thermosensitive microemulsion gel incorporating nano-ZnO and black soybean tar improves treatment adherence and alleviates psoriasis-like skin disease.

Tar has been used as a natural product in the treatment of psoriasis for thousands of years and has achieved good clinical therapeutic effects. This study aimed to develop black soybean tar (BST)-zinc oxide (ZnO) composite thermosensitive microemulsion gels (BST-ZnO-MBGs) to improve the properties of BST, increase patient compliance, and enhance drug treatment effects. BST-ZnO20/500/3000-MBGs containing different sizes of ZnO were prepared by high pressure homogenization technology, and the rheology, zinc ion release and transdermal mechanism of the gels were studied.

Targeting epigenetic regulators to overcome drug resistance in the emerging human fungal pathogen Candida auris.

The rise of drug-resistant fungal species, such as Candida auris, poses a serious threat to global health, with mortality rates exceeding 40% and resistance rates surpassing 90%. The limited arsenal of effective antifungal agents underscores the urgent need for novel strategies. Here, we systematically evaluate the role of histone H3 post-translational modifications in C.

Facilitators and barriers in managing older chronic heart failure patients in community health care centers: a qualitative study of medical personnel's perspectives using the socio-ecological model.

Background: Community health care centers (CHCs) plays a crucial role in ensuring timely diagnosis and effective management of congestive chronic heart failure (CHF) in older patients. Understanding the current status of CHF management in CHCs can therefore be effective in reducing the disease burden of CHF.

Objectives: This study evaluates the current state of CHF services in community healthcare facilities and identifies key facilitators and obstacles faced by medical personnel in China.

Associations between five indicators of epigenetic age acceleration and all-cause and cause-specific mortality among US adults aged 50 years and older.

Background: Although DNA methylation age estimators (DNAmAges) are reliable tools for predicting aging, their effectiveness in predicting mortality risk has not been fully validated. This study compared the predictive utility of five different DNAmAges (HorvathAge, HannumAge, PhenoAgeAge, GrimAge and GrimAge2) for all-cause and cause-specific mortality among adults aged ≥ 50 years.

Methods: We screened 1966 participants adults aged ≥ 50 from the National Health and Nutrition Examination Survey (1999-2002) and linked them to the National Death Index to obtain cause and status of death.

Causal relationship between graves' disease and mental disorders: A bidirectional Mendelian randomization study.

Objective: Many patients with Graves' disease (GD) also suffer from mental disorders in clinical practice, but their causal relationship remains unclear. This study aims to investigate the causal relationship between GD and common mental disorders using a bidirectional Mendelian randomization (MR)approach.

Methods: We derived genome-wide association study (GWAS) data for common mental disorders, including major depressive disorder (MDD), anxiety disorders, bipolar disorder, and attention-deficit/hyperactivity disorder (ADHD), from the Psychiatric Genomics Consortium consortium.

Strategies for Reducing the Homogenization Phenomenon in the Screening of Key Components in Network Pharmacology Based on the Detectability of Components.

Background: The ingredients of traditional Chinese medicine (TCM) are complex and diverse and are often identified using the TCM systems pharmacology (TCMSP) database. However, the ingredients in this database exhibit significant homogeneity, which limits the comprehensive understanding of TCM's ingredient diversity and biological activity. Consequently, liquid chromatography-mass spectrometry (LC-MS) has become a key tool for the precise identification of TCM components.

Multi-axis robotic forceps with decoupled pneumatic actuation and force sensing for cochlear implantation.

Delicate manual microsurgeries rely on sufficient hands-on experience for safe manipulations. Automated surgical devices can enhance the effectiveness, but developing high-resolution, multi-axis force-sensing devices for micro operations remains challenging. In this study, a 6-axis force-sensing pneumatic forceps with a serial-parallel robotic platform for cochlear implantation is developed.

Multiomics analysis of O-GlcNAcylation in podocytes of diabetic kidney disease.

Aim: To investigate the role of O-GlcNAc transferase (OGT)-mediated protein O-GlcNAcylation in podocyte injury during the progression of diabetic kidney disease (DKD).

Materials And Methods: Proteomic and O-glycoproteomic analyses were conducted on high glucose (HG)-stimulated podocytes with OGT knockdown. Differentially expressed proteins and O-GlcNAcylated peptides/proteins were identified, and functional enrichment (GO, KEGG, COG/KOG) and motif analysis (motif-x) were performed using bioinformatics analysis.

Highly Sensitive and Interference-Free Detection of Multiple Drug Molecules in Serum Using Dual-Modified SERS Substrates Combined with AI Algorithm Analysis.

Surface-enhanced Raman spectroscopy (SERS) technology has shown broad potential in drug concentration detection, but its application in blood drug monitoring faces significant challenges. The primary difficulty lies in overcoming the interference caused by various biomolecules present in serum, which can severely obscure the SERS signals of target drug molecules. Traditional enhancement substrates are often limited to detecting single drugs and are prone to interference, making the label-free detection of multiple drugs particularly challenging.

Sparse wavefield reconstruction based on Physics-Informed neural networks.

In recent years, the widespread application of laser ultrasonic (LU) devices for obtaining internal material information has been observed. However, this approach demands a significant amount of time to acquire complete wavefield data. Hence, there is a necessity to reduce the acquisition time.

Comparison of the efficacy and adverse effects of long pulsed 1064 nm Nd: YAG laser and sclerotherapy in the treatment of pyogenic granuloma in children: a retrospective study.

Pyogenic granuloma (PG) is benign vascular lesions of the skin and mucous membranes that often involve the skin and mucous membranes, which often trouble patients due to its frequent bleeding. The traditional treatment is surgical removal, but its bleeding, pain, and trauma have led doctors to look for more minimally invasive methods. Between June 1, 2022 to March 1, 2024, we retrospectively analyzed 72 children with PG who were seen and treated in our department for long pulsed 1064 nm Nd: YAG laser (Gentle Nd: YAG laser) and sclerotherapy, respectively.

Historically Polluted Area Increases Human Exposure Risks to Polychlorinated Naphthalenes through Waterfowl Egg Consumption.

Polychlorinated naphthalenes (PCNs), which are ubiquitous in the environment, are listed as persistent organic pollutants under the Stockholm Convention. Poultry can be exposed to PCNs via feed and breeding environments, leading to PCNs accumulation in eggs. However, information on PCNs in eggs from waterfowl raised in contaminated regions is scarce.

Study on chemical composition, anti-inflammatory activity and quality control of the branch bark of Morus alba L.

In this study, the chemical constituents of branch bark and root bark of Morus alba L. were first qualitatively analyzed by UPLC-Q/TOF-MS. A total of 40 compounds were identified, including 20 flavonoids, 3 stilbenes, 1 phenolic acid, 1 alkaloid, 5 arylbenzofurans and 10 Diels-Alder adducts.

A new therapy for microcystic lymphatic malformations: the combination of intralesional laser thermolysis and percutaneous sclerotherapy.

Background: Over the past few decades, percutaneous sclerotherapy has been proven to be efficacy in treating macrocystic lymphatic malformations (LMs). Unfortunately, there still remains challenging in the treatment of microcystic LMs given their size. We introduce the intralesional laser thermolysis (ILT) technique, a novel enhancement technique for the traditional percutaneous sclerotherapy in the treatment of microcystic LMs.

Efficacy and safety of propranolol tablets vs. oral solution in neonates with severe infantile hemangiomas: a retrospective study in China.

The objective of this study was to evaluate the efficacy and safety of propranolol hydrochloride tablets and oral solution in neonates with severe IHs. A retrospective cohort study included 184 consecutive neonates diagnosed with severe IHs and treated with propranolol from January 2016 to June 2023. Of these, 126 patients received propranolol tablets, and 58 received propranolol oral solution.

Flux-Screened Copper/Electric Dual-Catalyzed Chemo- and Enantioselective Ullmann-Type C-C Coupling Reactions.

This study introduces an innovative copper/electric dual-catalyzed approach to Ullmann coupling reactions. Our research delineates a series of chemoselective cross-couplings among various halogenated aromatics and enantioselective couplings involving halogenated aryl aldehydes. We employed a flux screening technique to refine the reaction parameters, which is rarely reported in the field of electrochemical synthesis.