POU6F1 promote lumbar motor circuit reorganization following spinal cord injury.

Spinal cord injury (SCI) causes irreversible motor deficits due to disrupted lumbar circuitry. However, transcriptional mechanisms in distal lumbar circuits are poorly understood. We identify POU6F1 as a critical transcriptional regulator in spinal lumbar segment (SLS, L3-L5) motor circuit regeneration.

Health literacy model integrating health education, health behaviors, self-rated health, and socioeconomic status in the Chinese population.

Health literacy is crucial for influencing health behaviors and outcomes; however, its development in China has been hindered by the lack of effective measurement tools and conceptual models. This study aimed to evaluate health literacy among Chinese residents using the HLS-Q47 and construct a health literacy model. A cross-sectional survey was conducted in Guangzhou from February to June 2023 involving 4047 participants.

Associations of serum iron and its status change with mortality risk: prospective findings from the MJ cohort.

Previous studies on serum iron levels and mortality risk have yielded inconsistent findings based on single-point measurements. How serum iron levels and their longitudinal changes influence all-cause and cause-specific mortality remains unknown. This study investigated associations between baseline serum iron levels, their longitudinal changes, and all-cause and cause-specific mortality in a prospective cohort.

Salidroside ameliorates abnormalities in electrophysiological indices induced by perfusion of the heart with low-potassium solutions.

Malignant arrhythmias related to hypokalemia are a key risk factor for cardiac arrest, but the specific mechanism remains unclear. In this study, using electrophysiological mapping and transcriptomics techniques, the effects of hypokalemia and paclitaxel (SAL) on isolated rat hearts were investigated. Hypokalemia (3.

Noncanonical terpene cyclases for the biosynthesis of diterpenoids regulating chlamydospore formation in plant-associated Trichoderma.

Terpene cyclases catalyze exquisite and complicated cyclization reactions to generate diverse terpenoid skeletons. Trichoderma fungi are important biocontrol agents, characteristic of producing complex bioactive tetracyclic diterpenoids named harzianes and trichodermanins, but their biosynthesis and biological functions have long been enigmatic. Here we identify TriDTCs, an unprecedented family of terpene cyclases in Trichoderma, responsible for cyclizing geranylgeranyl diphosphate (GGPP) into major diterpenes harzianol I and wickerol A, via heterologous expressions, gene deletion, and in vitro assays.

Dynamically monitoring pneumonia severity scores to predict the prognosis in patients with community-acquired pneumonia: an international, multicenter cohort study.

Background: Traditional pneumonia severity scores are widely used at treatment initiation to stratify disease severity and predict prognosis. Although they can be reassessed during treatment to evaluate disease progression, their predictive accuracy remains suboptimal. To address this limitation, we introduce a novel machine learning algorithm that quantifies continuous disease progression, enhancing real-time evaluation of treatment efficacy and outcome prediction in community-acquired pneumonia (CAP).

MicroRNA participates in embryo implantation by modulating endometrial tolerance in sows during peri-implantation period.

MicroRNAs (miRNAs) are a class of non-coding RNA. MiRNAs affect physiological processes by regulating messenger RNA (mRNA) translation of target genes. The peri-implantation period is the period with the most loss of pig embryos, during which the endometrium provides support for embryo selectivity.

Electrophysiological consequences of acute mobocertinib exposure in isolated rat and guinea-pig hearts and transfected cell lines.

Background: Mobocertinib is a first-in-class oral epidermal growth factor receptor (EGFR) tyrosine-kinase inhibitor approved for NSCLC with EGFR exon 20 insertions. Clinical trials indicate a signal for QT prolongation and heart failure, but the underlying mechanisms remain undefined. We used ex-vivo hearts and transfected cell lines to determine direct, acute electrophysiological consequences of mobocertinib in animal models.

Non-coding genetic elements of lung cancer identified using whole genome sequencing in 13,722 Chinese.

A substantial portion of lung cancer-associated genetic elements in East Asian populations remains unidentified, underscoring the need for large-scale genome-wide studies, particularly on non-coding regulation. We conducted a whole genome sequencing (WGS)-based genome-wide scan in 13,722 Chinese individuals to identify regulatory elements associated with lung cancer. We verified common-variant-based loci by meta-analysis across the available East Asian studies.

Associations of air pollution with semen quality and male infertility: A large-scale multicenter study in China.

Air pollutants have been linked to adverse effects on male reproductive health. However, large-scale multicenter studies on the association of air pollutants with male infertility and semen quality remain scarce. Here, we retrospectively collected data from 25,682 men at three reproductive centers in eastern China from 2015 to 2022 to investigate the relationships between air pollution exposure, semen quality and male infertility risk at different stages of sperm development, and whether semen quality mediates the relationship between air pollutants and male infertility risk.

Multifunctional litchi husk nanocellulose-reinforced bidirectional polymeric hydrogel for sequential therapy of infected wound.

The poor mechanical strength and singular therapeutic function of conventional hydrogel dressings have significantly constrained their clinical applicability for treating infected wounds. Consequently, the development of intelligent non-antibiotic dressings that exhibit both high mechanical strength and multiple synergistic therapeutic mechanisms represents a significant challenge. To address this, a "double polymerity-wide crosslinking" strategy was proposed in this work.

Development and Validation of a Brain Aging Biomarker in Middle-Aged and Older Adults: Deep Learning Approach.

Background: Precise assessment of brain aging is crucial for early detection of neurodegenerative disorders and aiding clinical practice. Existing magnetic resonance imaging (MRI)-based methods excel in this task, but they still have room for improvement in capturing local morphological variations across brain regions and preserving the inherent neurobiological topological structures.

Objective: To develop and validate a deep learning framework incorporating both connectivity and complexity for accurate brain aging estimation, facilitating early identification of neurodegenerative diseases.

Development and validation of deep learning- and ensemble learning-based biological ages in the NHANES study.

Introduction: Conventional machine learning (ML) approaches for constructing biological age (BA) have predominantly relied on blood-based markers, limiting their scope. This study aims to develop and validate novel ML-based BA models using a comprehensive set of clinical, behavioral, and socioeconomic factors and evaluate their predictive performance for mortality.

Methods: We analyzed data from 24,985 participants in the National Health and Nutrition Examination Survey (NHANES) from 1999 to 2010, with follow-up extending to 31 December 2019, or until death or loss to follow-up.

Bibliometrics and Visualization Analysis of Three Obligate Organohalide Respiring Bacteria Genera: A Systematic Review.

Organohalide-respiring bacteria (OHRB) facilitate the reductive dehalogenation of toxic halogenated compounds in the environment, which supports their growth and proliferation. Research conducted on OHRB has achieved notable advancements. However, given the intricacy of the ecosystem and the methodologies employed for microbial isolation, numerous constraints persist.

Whole Genome Sequence Analysis Revealed Novel Subjective Cognitive Decline-associated Genes in 10,763 Chinese.

Subjective cognitive decline (SCD) is widely regarded as a potential preclinical stage of Alzheimer's disease (AD), yet its genetic basis remains poorly understood. To address this gap, we investigated genetic biomarkers associated with SCD using whole-genome sequencing (WGS) in 10,763 Chinese participants from the Healthy Zhejiang One Million People Cohort (HOPE Cohort). The discovery stage included 9284 samples, with 1479 samples used for validation.

confined construction of BiVO/pg-CNdirect-heterostructures and its efficient degradation and photodynamic sterilization of rhodamine under visible light.

Photocatalytic degradation is increasingly recognized as a highly effective approach for the removal of organic pollutants and pathogenic microorganisms from wastewater. Nevertheless, conventional unit catalysts often fall short of practical requirements, primarily due to their limited efficiency in photoinduced electron-hole transfer and the scarcity of active sites. In this work, three-dimensional porous material pg-CNwas synthesized utilizing the hard template method, employing dendritic mesoporous silica as the templating agent.

Novel subtypes of metabolic associated steatotic liver disease linked to clinical outcomes: implications for precision medicine.

Background & Aims: Although metabolic dysfunction-associated steatotic liver disease (MASLD) is associated with high multimorbidity and mortality, existing classification systems and risk prediction models largely ignore the heterogeneity of MASLD. Improved subtype definition could improve prediction of outcomes and inform new precision treatment strategies.

Methods: We analyzed individuals with MASLD from population-based electronic health record resource from UK Biobank (n = 125,197) and Health examinee dataset of Nanfang Hospital (n = 995).

Exploring the impact of neutrophils on lung adenocarcinoma using Mendelian randomization and transcriptomic study.

Tumor immune microenvironment plays a crucial role in determining the prognosis of lung adenocarcinoma (LUAD), with the interaction of immune cells within this microenvironment contributing to a poorer prognosis. We sought to investigate the causal relationship and underlying biological mechanisms between immune cell characteristics and LUAD to offer new insights for enhancing treatment strategies. We evaluated the association between immune cell characteristics and LUAD using Mendelian randomization (MR) analysis based on genome-wide association studies summary statistics.

Lifestyles Modify the Biological Aging Process: Evidence from Multiple Cohorts.

The impact of lifestyle factors on biological aging is typically assessed one time at enrollment in epidemiological studies, with few studies examining their impact over time. In this prospective study with 143,823 individuals from the Taiwan MJ cohort, we investigate the association between healthy lifestyle scores (HLS) and annualized changes in biological age (ΔMDAge/year), replicated with Phenotypic Age and UK Biobank data. Compared to individuals with HLS of 0, increasing HLS was associated with decreased level of ΔMDAge/year, with the adjusted betas (95%CI) were -0.

The silkworm: A promising invertebrate diabetes model for natural drug discovery.

As an economically important insect, the silkworm () occupies a central position in the silk industry. Its unique physiological characteristics make it a potential model animal for research on disease modeling and drug screening. The aim of this review was to explore the feasibility of the silkworm as a model animal for diabetes and to evaluate the potential application of this model in new drug discovery.

Circadian gene BMAL1 ameliorates renal ischaemia-reperfusion injury in diabetic mice by enhancing mitophagy via the HIF-1/BNIP3 pathway.

Diabetic kidneys are particularly vulnerable to ischemia/reperfusion injury (I/RI). Although previous research has suggested that the circadian gene brain and muscle ARNT-like 1 (BMAL1) plays a role in regulating renal function, the exact functions and mechanisms of BMAL1 in diabetic renal I/RI remain elusive. In this study, bilateral renal artery ligation and release were performed in non-diabetic (db/+) and diabetic (db/db) mice.

ALKBH5-Mediated MA Demethylation of G3BP1 Attenuates Ferroptosis Via Cytoplasmic Retention of YBX1/p53 in Diabetic Myocardial Ischemia-Reperfusion Injury.

The overexpression of ALKBH5 alleviates damage to cardiomyocytes and tissues, decreases the expression of SLC7A11, and inhibits the expression of p53 and ferroptosis. ALKBH5 induces ferroptosis in the HH/R model by mutating the 3'-UTR of G3BP1 mRNA and mA sites at positions 142 and 173. Mutations of sites 142 and 173 only partially affect the levels of p53, SLC7A11 and ferroptosis.

Lidocaine as a Potential Therapeutic Agent in Colorectal Cancer: A Study of Gene Expression and Prognosis.

Background: Colorectal cancer (CRC) is a significant contributor to cancer-related mortality globally. Despite the availability of treatments such as surgery, chemotherapy, and radiotherapy, these interventions are often accompanied by severe side effects and suboptimal patient outcomes. Recent studies have suggested that lidocaine, a widely used local anesthetic, may possess anti-tumor properties in various cancer types.

Corrigendum to "Predicting all-cause mortality and premature death using interpretable machine learning among a middle-aged and elderly Chinese population" [Heliyon Volume , Issue 17, September 15 2024, Article e36878].

[This corrects the article DOI: 10.1016/j.heliyon.

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants.

Bud-sport mutations are pivotal for enhancing fruit quality traits in horticultural crops. Clarifying the metabolic differences between pears and their red bud-sport variants can help the selection of high-quality red pear resources, but there are fewer related studies. In this study, we investigated how bud-sport modulates flavonoid metabolic networks and affects fruit phenotypic differentiation.