Au(III) Schiff base complexes as oxidoreductase inhibitors against carbapenem- and colistin-resistant Gram-negative bacteria via targeting redox active motifs.

Carbapenem- and colistin-resistant Gram-negative bacteria have become one of the most severe public health issues worldwide. The development of advanced antibacterial agents that can outpace microbial adaptation is imperative. The thioredoxin (Trx) and glutaredoxin (Grx) systems play important roles in maintaining redox homeostasis within Gram-negative bacterial cell membranes, with thioredoxin reductase (TrxR) and glutathione reductase (GR) being classical antibacterial targets.

Construction of discharge preparation service for patients undergoing cataract day surgery based on evidence and its empirical application.

Objective: Evidence-based development and empirical application of a discharge preparation service program for cataract day surgery patients.

Methods: Evidence-based construction of a discharge preparation service programme for cataract day surgery patients. Using the convenience sampling method, 84 patients undergoing cataract day surgery in July-August 2024 were divided into a control group and an intervention group; Comparing the level of readiness for hospital discharge, quality of discharge teaching, caregiver preparedness, patients' ability to self-care, complication rate and unplanned readmission rate 1 month post-discharge between the two groups.

Three tetraspanins, Tspan1, Tspan2A and Tspan3 from Litopenaeus vannamei involved into Vibrio parahaemolyticus infection.

Tetraspanins have essential roles in immune defense in eukaryotes. However, the research of tetraspanins in the immune system of crustaceans is relatively limited. Our previous transcriptome analysis found that the expression of three members of the tetraspanin family - Tspan1, Tspan2A and Tspan3 - was significantly changed in Litopenaeus vannamei after Vibrio parahaemolyticus infection.

Case report and literature review: novel TXNRD2 compound heterozygous variants in familial glucocorticoid deficiency type 5.

Familial glucocorticoid deficiency (FGD) is a rare autosomal recessive disorder characterized by isolated glucocorticoid deficiency. Mutations of , , , , and have been implicated in FGD pathogenesis. To date, only four families with associated familial Glucocorticoid Deficiency Type 5 (FGD5) have been reported worldwide.

Dual function of a zinc finger transcription factor PvBr-C in Penaeus vannamei.

The Broad-Complex (Br-C) is a pivotal transcription factor in arthropod development and metamorphosis, but its functional roles in crustaceans remain poorly understood. In this study, we identified and characterized a Br-C homolog (PvBr-C) in Penaeus vannamei through molecular cloning, phylogenetic analysis, and functional investigation. The 2343-bp cDNA encoded a 781-amino-acid protein containing conserved BTB_POZ and zinc finger domains, exhibiting high homology with the analogous sequences in decapod crustaceans.

Shrimp plasma calcyphosine enhances hemocyte immune responses by promoting glycolysis.

Calcyphosine, a highly conserved calcium-binding protein found in metazoans, has been recognized as a cell cycle regulator associated with poor cancer prognosis. However, its extracellular functions remain largely unknown. Here, we investigated its role as a plasma protein in the marine invertebrate Litopenaeus vannamei.

Establishing an algorithm for molecular genetic diagnostics in Chinese children with brachydactyly type E.

Background: Brachydactyly type E (BDE) is characterized by variable shortening of metacarpals or metatarsals, often involving phalanges. It may occur as an isolated anomaly or as part of congenital syndromes. With advancements in molecular diagnostic technologies, how genetic testing enhances the precise diagnosis of BDE remains unclear.

Enhanced removal of tetracycline and doxycycline in aquaculture pond sediments via synthetic bacterial consortium: Efficacy and ecological risk assessment.

Tetracycline antibiotics (TCs), extensively used in aquaculture and livestock production, pose significant ecological risks due to their accumulation in aquatic sediments. This study investigated the efficacy and ecological implications of bioaugmentation with a synthetic microbial consortium for the remediation of TCs-contaminated aquaculture pond sediment. Sediment microcosms were amended with tetracycline (TC) and doxycycline (DOX) at different dosages, with or without consortium inoculation.

Smart ultra-long-lasting sequentially triggerable and artfully implantable nozzle system for on-demand drug delivery for chronotherapy.

Conventional drug delivery methods for chronic disease often suffer from low potency and poor patient compliance, while current advanced devices face limitations because of bulkiness, frequent implantation needs, inflammation risk, and lack of precise control. To overcome these challenges, we developed the SUSTAIN-a smart, ultra-long-lasting, sequentially triggerable, and artfully implantable nozzle system. The SUSTAIN integrates an osmotic pressure-triggered module, an airflow-generated T-pipe (AGT), and a drug infusion pump (DIP) for controlled subcutaneous drug release.

Allostatic load partially mediates the association between number of stressful life events and cardiometabolic multimorbidity: A nationally representative cross sectional study from CHARLS.

Background: Stressful life events are independent psychological risk factor for diabetes and cardiovascular disease, yet studies on cardiometabolic multimorbidity (CMM) are scarce. This study aimed to examine the effects of stressful life events and their accumulation on the prevalence of CMM, as well as the mediating role of allostatic load in the association between accumulated stressful life events and CMM.

Methods: A total of 7493 participants from Wave 3 (2015) of the China Health and Retirement Longitudinal Study (CHARLS) were included.

Validation of the Chinese version of the financial toxicity scale in patients with wet age-related macular degeneration.

Objective: to confirm the validity of the Comprehensive Score for Financial Toxicity (COST) Scale in Chinese patients with wet age-related macular degeneration.

Methods: A tertiary hospital in Wuxi's ophthalmology outpatient clinic treated 217 patients with wet age-related macular degeneration (wAMD) were chosen using the convenience sample approach for a questionnaire survey between October 2023 and February 2024. The Chinese version of the COST and general patient information were included in the survey.

Circadian clocks and their role in kidney and eye diseases across organ systems.

Circadian rhythms, the body's intrinsic 24-h cycles, regulate essential physiological processes across multiple organ systems. Disruptions in these rhythms are increasingly recognized as significant contributors to chronic kidney disease and ocular diseases such as glaucoma, dry eye disease, and diabetic retinopathy. This review examines the interconnections between circadian rhythms in kidney and eye health, focusing on shared pathological pathways including oxidative stress, inflammation, and fibrosis.

Mannose-modified hemocyanin enhances pathogen endocytosis by crustacean hemocytes.

In crustaceans, hemolymph plasma contains more than 90% hemocyanin, whereas hemocytes have minimal levels, suggesting a regulated uptake mechanism. Here, we demonstrate that in Penaeus vannamei, hemocytes internalize plasma hemocyanin under normal conditions via phagocytosis, clathrin-mediated endocytosis, and micropinocytosis. This uptake is significantly enhanced during bacterial (Vibrio parahaemolyticus, Vibrio alginolyticus, Staphylococcus aureus, Streptococcus iniae) and viral (White spot syndrome virus) infections or upon stimulation with pathogen-associated molecular patterns.

Stochastic processes dominate the community assembly of ectomycorrhizal fungi associated with in Inner Mongolia, China.

The maintenance and driving mechanisms of microbial community structure have become important research focuses in microbial ecology. Therefore, clarifying the assembly of ectomycorrhizal (EM) fungal communities can provide a relevant basis for studying forest diversity, ecological diversity, and ecological evolution. is a typical EM dependent tree species with characteristics such as renewal ability and strong competitive adaptability, and it plays a crucial ecological function in Inner Mongolia.

Changes in gut microbiota and physiological functions of mud crab (Scylla paramamosain) in response to Vibrio parahaemolyticus infection.

The mud crab (Scylla paramamosain) is a major aquaculture species in southeastern China. However, intensifying farming has led to disease outbreaks, with Vibrio parahaemolyticus (Vp) infections causing high mortality and economic losses. Although gut microbiota is important for host health, its response to artificial Vp challenge in mud crabs remains poorly understood.

Self-Powered Algae-Integrated Wearable System for Oxygen Supplementation in Hypoxic Disease Treatment.

Hypoxia serves as a critical determinant in the advancement of various intractable pathological conditions including oncological disorders and hypovascular wounds, which may profoundly attenuate the efficacy of pharmacological interventions and substantially inhibit the physiological recovery processes. Consequently, in an effort to mitigate the inherent constraints of conventional methodologies (, exogenous oxygen delivery systems), a self-powered triboelectric nanogenerator (TENG)-based algae-integrated pliable and enveloped device (TAPED) operates as a wearable system to sustain oxygen generation. The TAPED system harnesses biomechanical energy generated through natural bodily movements to energize an integrated luminescent source, enabling controlled photosynthesis for sustained, on-demand oxygen production.

FLT3 ligand regulates expansion of regulatory T-cells induced by regulatory dendritic cells isolated from gut-associated lymphoid tissues through the Notch pathway.

Background: Regulatory dendritic cell (DCreg) subset exhibits a unique capacity for inducing immune tolerance among the variety subsets of dendritic cells (DCs) within gut-associated lymphoid tissues (GALTs). Fms-like tyrosine kinase 3 ligand (FLT3L) is involved in the differentiation of DCregs and the subsequent expansion of regulatory T-cells (Tregs) mediated by DCregs, though the precise mechanism remains poorly understood. This study aimed to explore the expansion mechanism of Treg induced by DCreg and the role of FLT3L in this process.

Kinetics of pIgR and IgM immune responses in snakehead (Channa argus) to inactivated Aeromonas hydrophila via immersion and intraperitoneal injection.

This research was intended to investigate the kinetics of polymeric immunoglobulin receptor (pIgR) and its ligand immunoglobulins (Ig) in snakehead (Channa argus) through different vaccine delivery methods. First, we employed Oxford Nanopore Technologies (ONT) sequencing technology to acquire the snakehead fish's complete transcriptome, and the full coding sequence (CDS) of pIgR was identified and isolated through transcriptome sequencing named ChpIgR. The CDS sequence spanned 1251 base pairs, translating into a protein that consists of 416 amino acids and has a molecular weight of around 45.

Rapid and sensitive detection of cell surface N-glycans in live cancer cells using a novel acid-activated concanavalin A nanosensor.

Glycans on the surface of cells play critical roles in various biological processes, including cell communication, the immune response, infection, development, and differentiation. The presence of glycans is closely related to cancer growth and metastasis. This study presents a biosensor based on an acid-activated nanoplatform for glycan detection on live cancer cells.

Professional benefits perception among pediatric nurses: a cross-sectional survey.

Background: The perception of professional benefits is a critical determinant of job stability and work efficacy among healthcare professionals. This perception can directly influence patient care quality, nurse job satisfaction and retention, as well as overall organizational efficiency. The current understanding of how pediatric nurses perceive their professional benefits is limited.

Maternal exposure to polystyrene nanoplastics induces sex-specific kidney injury in offspring.

Maternal exposure to polystyrene nanoplastics (PS-NPs) during pregnancy and lactation has been linked to adverse effects on offspring kidney development, with sex-specific outcomes. This study investigated the impact of maternal PS-NPs exposure on kidney weight, histology, transcriptomics, and functional pathways in offspring mice. Offspring exposed to PS-NPs exhibited significantly lower body weight (P < 0.

Z/Ce@hemin enzymes with enhanced peroxidase activity for monitoring and screening the oxidative stress models of Parkinson's disease.

Inspired by natural enzymes, artificial enzymes have garnered significant interest due to their simplicity of production, robustness under harsh conditions, and enhanced stability. This study introduces, for the first time, a novel Z/Ce@hemin composite enzyme, constructed by anchoring hemin onto zeolitic imidazolate framework-8 (ZIF-8)-encapsulated ceria (CeO) nanoparticles. This innovative design overcomes the challenges of hemin dimerization, enhances substrate affinity, and accelerates mass transport, leading to significantly improved peroxidase-like activity.

Shrimp Shapes a Nitrite Tolerance Trait via Regulating Autophagy and Apoptosis.

Nitrite is a significant toxic substance that causes acute toxicity in aquatic animals. However, the molecular mechanisms underlying nitrite tolerance remain largely unidentified. Here, we investigated the physiological and transcriptomic profiles of nitrite-sensitive and nitrite-tolerant families of the white shrimp under nitrite stress.

Effect of surgery on overall survival and cancer-specific survival in patients with primary HCC: A study based on PSM in the SEER cohort.

This study was designed to assess the effects of surgery method on overall survival (OS) and cancer-specific survival (CSS) in patients with hepatocellular carcinoma (HCC). This is a retrospective study. Patients diagnosed with primary HCC (N = 10,174) were identified from the Surveillance, Epidemiology, and End Results Database from 2010 to 2017 and categorized into surgical (N = 4950) and nonsurgical (N = 5224) groups.