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Article Abstract
Background: spinal cord injury (SCI) causes irreversible motor and sensory deficits with limited effective treatments. Mesenchymal stromal cells (MSCs) exert therapeutic effects largely through extracellular vesicles (EVs). Preconditioning MSCs with a hydrogen sulfide (HS) donor enhance the therapeutic potential of EVs.
Objective: this study is aimed to develop a 3D-printed gelatin methacryloyl (GelMA) scaffold loaded with HS-preconditioned MSC-derived EVs (HS-EVs) to promote motor function recovery in SCI.
Methods: HS-EVs were isolated from NaHS (an HS donor)-preconditioned MSCs and incorporated into a 3D-printed GelMA scaffold (3D/GelMA/EVs). Scaffold mechanical properties and HS-EVs. The scaffold's therapeutic efficacy was evaluated in a rat SCI model.
Results: MiRNA microarray revealed miR-7a-5p as the most upregulated miRNA in HS-EVs. The 3D/GelMA/EVs scaffold exhibited an appropriate elastic modulus and porous structure, enabling sustained local EVs release. In vivo, the scaffold significantly improved motor function recovery in SCI rats.
Conclusion: these results indicated that HS-EVs provided an important therapeutic tool against SCI by miR-7a-5p and 3D/GelMA/EVs scaffolds were ideal biomaterials for the intervention of SCI.
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| http://dx.doi.org/10.1021/acsbiomaterials.5c00083 | DOI Listing |
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