Label-free immunoassay based on chemiluminescence-functionalized magnetic mesoporous nanoparticles for rapid detection of neuron-specific enolase.

Lung cancer, as one of the cancers with the highest morbidity and mortality rates in the world, requires accurate detection of its vital serum marker, neuron-specific enolase (NSE), which is a key challenge for early detection of lung cancer. However, traditional chemiluminescence immunoassay (CLIA) methods rely on labeled antibodies (Abs) and suffer from complex operations and high costs. In this work, a label-free CLIA based on CL-functionalized mesoporous magnetic nanoparticles (CuFeO@mSiO-Cys-Luminol-Au NPs) is developed for the rapid and sensitive detection of NSE.

Targeting HMGB1 modulates cancer-associated fibroblasts and enhances radiotherapy in lung adenocarcinoma.

Radio-resistance remains a major challenge in the effective treatment of lung cancer. Cancer-associated fibroblasts (CAFs), the predominant cellular components in solid tumors, play a crucial role in tumor treatment and resistance. Thus, understanding the interactions between CAFs and tumor cells is key to overcoming radio-resistance in lung cancer.

Association between hemoglobin albumin lymphocyte and platelet (HALP) score and psoriasis: Evidence from NHANES.

Background: The HALP (hemoglobin, albumin, lymphocyte, and platelet) score is a novel indicator that measures systemic inflammation and nutritional status. This study aimed to investigate the association between HALP score and psoriasis in US participants.

Methods: Data from the National Health and Nutrition Examination Survey (NHANES) (2003-2006, 2009-2014) were utilized.

Midterm outcomes of rheumatic mitral valve repair versus replacement in a dual-centre retrospective study.

Background: Rheumatic mitral valve disease remains a major global health challenge. Determining optimal surgical approaches is critical. This study aimed to identify key repairability factors and compare midterm outcomes of mitral repair versus replacement.

MPTP controls the release of mtDNA and induces endothelial cell PANoptosis in trichloroethylene-induced immune kidney injury.

Vascular endothelial cells (ECs) damage is closely related to kidney injury. Our previous research revealed the involvement of interferon regulatory factor 1 (IRF1)-mediated PANoptosis of renal ECs in trichloroethylene (TCE)-induced immune kidney injury. However, how IRF1 regulates ECs PANoptosis remains unclear.

ZnCe-LDO Nanozyme-Based Multifunctional Hydrogel Promotes Bone Regeneration by Inflammatory Macrophage Reprogramming and Piezo1 Activation.

Prolonged or excessive inflammation may lead to impaired vascularization and bone regeneration, hindering the normal repair process of bone tissue. Although the regulation of inflammation is crucial for promoting a conducive microenvironment for bone regeneration, individual anti-inflammatory interventions frequently are inadequate in facilitating effective bone repair. Here, a multifunctional hydrogel (GelMA-ZC-Yoda1) with multifaceted therapeutic strategy was designed by integrating Zinc/Cerium-layered double oxide nanozyme (ZnCe-LDO, with catalase-like activity) and Yoda1 (an activator of the mechanically sensitive Piezo1 ion channel) into photocurable GelMA hydrogel.

Interpretable machine learning model predicts 1-year inguinal hernia risk after robot-assisted radical prostatectomy.

Inguinal hernia represents a clinically significant yet underreported complication of robot-assisted radical prostatectomy (RARP) for localized prostate cancer, with a notably high incidence within the first postoperative year. Despite its adverse impact on quality of life and potential for severe sequelae, predictive tools for this outcome remain limited. To develop and validate the first machine learning (ML)-based clinical prediction model for inguinal hernia within 1 year after RARP, leveraging explainable artificial intelligence (AI) techniques for clinical interpretability.

Clinical characteristics of dyslipidemia in patients with primary biliary cholangitis: a single-center experience in China.

Aims: Dyslipidemia is frequently observed among individuals diagnosed with primary biliary cholangitis (PBC), though its specific characteristics remain incompletely defined. This study aimed to examine the lipid profile patterns and medical features of dyslipidemia in people suffering from PBC.

Methods: Following the classification criteria proposed by the National Lipid Association, dyslipidemia is classified on the basis of abnormal plasma concentrations of high-density lipoprotein cholesterol (HDL-C), total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C).

Multicenter Practice of Non/Minimized Fluoroscopy Ablation for Paroxysmal AF in China: The PAF-ICE Trial.

Background: Intracardiac echocardiography (ICE)-guided non/minimized-fluoroscopy catheter ablation for atrial fibrillation (AF) has been reported, but its effectiveness and safety still lack multicenter evidence.

Objectives: The authors sought to evaluate the effectiveness and safety of ICE-guided non/minimized-fluoroscopy catheter ablation compared with the traditional fluoroscopy-guided approach in patients with paroxysmal AF.

Methods: A total of 448 patients with paroxysmal AF, from 15 centers in China, were randomly assigned in a 1:1 ratio to a non/minimized-fluoroscopy group (n = 223) and a traditional approach group (n = 225).

Positive Metagenomic Next-Generation Sequencing of Renal Lavage Fluid Associates with Delayed Graft Function in Kidney Transplants from Donors After Circulatory Death: A Retrospective Study.

Background: Delayed graft function (DGF) is a major complication in kidney transplants from donation after circulatory death (DCD). This study assessed the association between metagenomic next-generation sequencing (mNGS) results and the occurrence of DGF during the perioperative period in DCD kidney transplant recipients.

Methods: We analyzed 191 DCD kidney transplant recipients in this single-center retrospective cohort study.

Evolutionary patterns and future perspectives of joint replacement in arthritis patients: a comprehensive analysis of findings over the past decades.

Purpose: This study aimed to comprehensively review the current research status and trends of joint replacement for arthritis patients worldwide.

Methods: Literature related to joint replacement for arthritis patients from 2004 to 2024 was extracted from the Web of Science Core Collection (WoSCC) database. A systematic qualitative and quantitative analysis of these publications was conducted.

Activation of Locus Coeruleus-Hippocampus Tyrosine Hydroxylase Projection Contributes to the Surgical Incision Pain-Induced Memory Consolidation Enhancement in Mice.

Aims: The mechanism underlying postoperative post-traumatic stress disorder (PTSD) remains unclear. However, studies have shown that acute postoperative pain is an independent risk factor for PTSD, which is also closely related to memory consolidation enhancement. Preoperative patients often experience unpleasant traumatic events, and postoperative pain usually occurs in the memory consolidation stage of these events.

Comprehensive pan-cancer analysis reveals ubiquitin-conjugating enzyme 2T as a potential biomarker for prognosis and cancer immunity.

Ubiquitin-conjugating enzyme 2T (UBE2T) constitutes a critical component of the ubiquitin-proteasome system and is involved in tumorigenesis. The gene has been extensively characterized. In the present study, comprehensive analyses using various databases and R-based tools revealed elevated expression across multiple tumor types, where its upregulation was shown to be associated with poor clinical outcomes and prognosis.

Effects of Dexmedetomidine on Postoperative Quality of Recovery and Electroencephalogram in Elderly Patients Undergoing Total Knee Arthroplasty: A Randomized Clinical Trial.

Purpose: Anesthesia management strategies in elderly patients undergoing total knee arthroplasty (TKA) are critical to the postoperative quality of recovery (QoR), and changes in electroencephalogram (EEG) associated with anesthesia drugs may play an important role in this process. This study aimed to determine the effects of different doses of dexmedetomidine on postoperative QoR in elderly TKA patients, and whether there is a correlation with specific EEG changes.

Methods: In this randomized controlled trial, elderly patients (aged ≥ 60 years) undergoing elective TKA were randomly allocated in a 1:1:1 ratio to 0.

TP53 drives neuronal ferroptosis by promoting KLHL4-mediated SLC7A11 ubiquitination after spinal cord injury.

Ferroptosis constitutes a pivotal pathological event following spinal cord injury and presents substantial challenges to the restoration of neurological function. Cystine-glutamate transporter SLC7A11 is essential for maintaining cellular redox homeostasis and resisting ferroptosis. However, the mechanisms underlying neuronal ferroptosis caused by SLC7A11 downregulation following spinal cord injury remain unclear.

Rosmarinic acid as a chemosensitizer in colorectal cancer: Targeting nuclear factor-kappa B pathway to overcome chemoresistance.

Colorectal cancer (CRC) is the third most common malignancy. However, the efficacy of current treatment strategies remains limited. In recent years, monomeric compounds from traditional Chinese medicine have received extensive attention in cancer therapy.

Effects of all-trans retinoic acid on glutamate receptor expression and dendritic spine density in the hypothalamus of rats.

Increasing evidence suggests that all-trans retinoic acid (ATRA) contributes to the pathogenesis of depression. Although numerous studies have emphasized the role of hippocampal glutamatergic transmission and dendritic spine abnormalities in ATRA-induced depression, it remains unclear whether changes in hypothalamic glutamatergic transmission and dendritic spine density also contribute to its development. This study investigates the effects of ATRA treatment on glutamate receptor expression and dendritic spine density in the hypothalamus of rats.

Deep learning-driven multi-hierarchical granularity integration for surgical scene understanding: experimental study.

Background: A comprehensive understanding of surgical scenes by computers is a crucial foundation for achieving intelligent surgical assistance and autonomous decision-making. Surgical scene information encompasses coarse-grained data reflecting the overall process and fine-grained details showcasing specific operations. This study aims to construct a standardized, full-grained annotation dataset for laparoscopic radical nephrectomy and develop a deep learning framework for multi-hierarchical granularity integration, providing support for clinical intelligent applications.

An electrochemical biosensor for breast cancer diagnosis based on RCA-enhanced metal-organic framework self-assembly.

Circular human epidermal receptor 2 RNA (circ-HER2) has been revealed to be overexpressed in HER2-positive breast cancer, which demonstrates the potential to serve as a potential prognostic biomarker for guiding the treatment of circ-HER2-positive breast cancer patients. But convenient, efficient and low-cost detection of circ-HER2 is rarely explored. Herein, we have fabricated an electrochemical biosensor for the accurate detection of circ-HER2 in breast cancer based on isothermal amplification-enhanced self-assembly of metal-organic frameworks (MOFs).

[Comparison of the efficacy of sigmoid sinus constriction plus cartilage reconstruction and sigmoid sinus return surgery in sigmoid sinus-related pulsatile tinnitus].

To investigate the efficacy of sigmoid sinus constriction plus cartilage reconstruction technique for sigmoid sinus related pulsatile tinnitus. The clinical data of 31 patients with Sigmoid Sinus Diverticulum（SSD） who underwent surgical treatment from January 2020 to December 2023 were retrospectively analyzed. The surgical methods include transmastoid sigmoid sinus constriction and cartilage reconstruction, transmastoid sigmoid sinus（bone wax） return surgery.

CFAP300 loss-of-function variant causes primary ciliary dyskinesia and male infertility via disrupting sperm flagellar assembly and acrosome formation.

Primary ciliary dyskinesia (PCD) is a genetically heterogeneous disorder characterized by impaired motility of cilia and flagella. Mutations in cilia- and flagella-associated protein 300 (CFAP300) are associated with human PCD and male infertility; however, the underlying pathogenic mechanisms remain poorly understood. In a consanguineous Chinese family, we identified a homozygous CFAP300 loss-of-function variant (c.

Lycium barbarum polysaccharides alleviate experimental autoimmune prostatitis by putrescine/TRAF/JAK/STAT-mediated Th17 differentiation.

Background: Due to its often refractory nature, chronic prostatitis (CP) is a prevalent disorder that notably impacts the quality of life of 20-40-year-old men worldwide. Lycium barbarum polysaccharides (LBPs) have a broad spectrum of biological activities, such as immune modulation and the ability to reduce blood glucose and lipid levels. However, whether they modulate the gut microbiota and metabolites to exert their anti-inflammatory effects remains to be explored.

Nano MgO loaded thermosensitive HPCH@HA hydrogel accelerates in situ bone repair through osteoimmunomodulation while enhancing angiogenesis and osteogenesis.

Bone defect repair is a complex physiological process, starting with early modulation by the inflammatory immune system, and involves multiple physiological events, including angiogenesis, osteogenic differentiation, and mineralization. Biomaterial can regulate inflammatory responses through relevant immune cells in the local immune microenvironment of the implant-bone interface which is a hot topic in the field of regenerative medicine. Currently, Mg regulates immune cells in the bone microenvironment to promote osteogenesis and angiogenesis mainly focuses on macrophages,but there is relatively little research on T cells.

Identification of proteomic markers of chronic prostatitis/chronic pelvic pain syndrome treated with melatonin using a tandem mass tag approach.

Background: Chronic prostatitis (CP)/chronic pelvic pain syndrome is the most common urological disorder in young and middle-aged men. A previous study showed that melatonin attenuates prostate inflammation through Sirt1-dependent suppression of the nonobese diabetic-like receptor thermal protein domain-associated protein 3 inflammasome in mouse models of experimental autoimmune prostatitis (EAP). However, the main differentially expressed proteins (DEPs) in melatonin-treated mice with EAP have not yet been fully identified.

Dual-ferroptosis induction-based microneedle patches for enhanced chemodynamic/photothermal combination therapy against triple-negative breast cancer.

Triple-negative breast cancer (TNBC) remains a refractory subtype of breast cancer due to its resistance to various therapeutic strategies. In this study, we introduce a "brake-release and accelerator-pressing" approach to engineer a microneedle patch embedded with copper-doped Prussian blue nanoparticles (Cu-PB) and the ferroptosis inducer sorafenib (SRF) for raised chemodynamic (CDT)/photothermal (PTT) combination therapy against TNBC. Upon transdermal insertion, the dissolving microneedles swiftly disintegrate and facilitate the release of SRF.