Label-free immunoassay based on chemiluminescence-functionalized magnetic mesoporous nanoparticles for rapid detection of neuron-specific enolase.

Lung cancer, as one of the cancers with the highest morbidity and mortality rates in the world, requires accurate detection of its vital serum marker, neuron-specific enolase (NSE), which is a key challenge for early detection of lung cancer. However, traditional chemiluminescence immunoassay (CLIA) methods rely on labeled antibodies (Abs) and suffer from complex operations and high costs. In this work, a label-free CLIA based on CL-functionalized mesoporous magnetic nanoparticles (CuFeO@mSiO-Cys-Luminol-Au NPs) is developed for the rapid and sensitive detection of NSE.

Identification of Candidate Gene Networks Controlling Soluble Sugar Metabolism During L. Development by Integrated Analysis of Metabolic and Transcriptomic Analyses.

Soluble sugars are among the key components determining the flavor quality of rapeseed bolting. However, the potential regulatory network governing the biosynthesis of soluble sugars during the growth and development of rapeseed bolting remains largely unknown. In this study, the total soluble sugar and starch contents were measured at the seedling and bolting stages in 203 germplasms.

Glucokinase activators contribute to gastrointestinal disease risks through metabolic-immune interplay in the gut-liver axis: insights from a multi-omics study.

Aims: Glucokinase activators (GKAs) lower glucose by directly activating glucokinase (GK) or dissociating it from GK regulatory protein (GK-GKRP). Their long-term effects on gastrointestinal (GI) diseases remain unclear. This study explores how two approaches influence GI disorders through metabolic-immune interplay.

Development of a Broad-Spectrum Antigen-Capture ELISA Using Combined Anti-p26 Polyclonal and Monoclonal Antibodies for Detection of Equine Infectious Anemia Virus.

Equine Infectious Anemia Virus (EIAV) poses significant diagnostic challenges due to its genetic variability and the limitations of conventional nucleic acid detection methods. This study developed an antigen-capture, enzyme-linked immunosorbent assay (AC-ELISA) for the detection and quantification of the EIAV capsid protein p26. The assay utilized a monoclonal antibody (1G11) specific to the p26 protein as the capture antibody and a polyclonal antibody as the detection antibody, forming a highly specific and sensitive detection system.

Visualized chemiluminescence immunoassay for simultaneous detection of multiple lung cancer markers based on spatially resolved microfluidic paper chip device.

Lung cancer ranks as the most prevalent form of cancer. Early detection and diagnosis can lower death rates, and variations in tumor markers can help assess cancer risk. In this work, a glow-type chemiluminescent (CL) hydrogel SCG-Co-luminol was synthesized by a simple approach, and this hydrogel was integrated with a 3D microfluidic paper-based analytical device (3D μPAD) to develop a visual biosensor for identifying multiple lung cancer markers.

Clofoctol impairs the stemness of gastric cancer and induces TNF-mediated necroptosis by directly binding to RanBP2.

Gastric cancer stem cells (GCSCs) play a crucial role in the initiation, progression, recurrence and therapeutic resistance, contributing to a poor prognosis. Consequently, GCSCs are deemed to be a potential therapeutic target for gastric cancer (GC). Although β-catenin is a well-recognized therapeutic target for GC and several inhibitors have demonstrated potent anti-tumor effects, there is a dearth of therapeutic agents targeting β-catenin for clinical therapy.

Microbial interactions between Lactoplantibacillus plantarum and Rhodotorula mucilaginosa in the fermented fish juice system.

Lactic acid bacteria and yeasts play important roles in fermented fish products. The present study investigated the interaction between Lactoplantibacillus plantarum and Rhodotorula mucilaginosa in a fermented fish juice system. The results demonstrated that the co-culture of L.

Multidimensional Nanostructures Design of Poly-Imidazolium Salts Derived N-Doped Carbon Materials for Electromagnetic Microwave Absorption.

Poly-imidazolium salt (PIS) combines the merits of abundant nitrogen sources, excellent chemical and thermal stability and adjustable morphology, making it a promising precursor for Nitrogen-doped (N-doped) carbon material. Herein, multidimensional PIS are synthesized via a facile solvent-induced strategy. Then, PIS are converted into nanofibers, nanoribbons and microspheres composed of N-doped carbon.

PRMT5 deficiency in myeloid cells reprograms macrophages to enhance antitumor immunity and synergizes with anti-PD-L1 therapy.

Background: Arginine methyltransferase protein arginine methyltransferase 5 (PRMT5) plays a significant role in immune regulation, particularly within the tumor microenvironment (TME). Macrophages are crucial modulators of both innate and adaptive immune responses, and their differentiation into tumor-associated macrophages is critical in shaping the TME. Despite ongoing clinical trials of small molecule inhibitors of PRMT5 for cancer therapy, their effects on macrophages, a key component of the immune system, remain poorly understood.

Multidimensional Co-Design and Performance-Mechanism Study of Novel Graphdiyne Composites with Microwave Absorbing Structures.

Graphdiyne (GDY), an emerging member in the carbon material family, possesses abundant chemical bonds, extended conjugated systems, and superior charge carrier mobility, establishing it as a promising novel microwave absorption material. Capitalizing on these attributes, in this work, a flower-like GDY@CuO composite with a unique nanowall structure is prepared by a one-step microemulsion method. Remarkably, temperature-mediated enhancement of electron transport coupled with induced multipolarization synergistically boosts the microwave absorption performance.

Identification and Validation of Four Serum Biomarkers With Optimal Diagnostic and Prognostic Potential for Gastric Cancer Based on Machine Learning Algorithms.

Background: Gastric cancer (GC) is considered a highly heterogeneous disease, and currently, a comprehensive approach encompassing molecular data from various biological levels is lacking.

Methods: This study conducted different analyses, including the identification of differentially expressed genes (DEGs), weighted correlation networks (WGCNA), single-cell RNA sequencing (scRNA-seq), mRNA expression-based stemness index (mRNAsi), and multiCox analysis, utilizing data from Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases. Subsequently, the machine learning algorithms including least absolute shrinkage and selection operator (LASSO) regression and random forest (RF), combined with multiCox analysis were exploited to identify hub genes.

Age-related abnormalities in brain functional and molecular neuroimaging signatures in first-episode depression.

Abnormalities in resting-state brain activity have been demonstrated in depression patients with different ages, yet the age-related changes in dynamics of brain activity in depression are still limited. Here, we investigated the impacts of age on dynamics of brain activity and the molecular architecture. Resting-state functional magnetic resonance images were obtained from 138 first-episode depression patients and 120 healthy volunteers.

Transcription factor Ste12 regulates the spore maturation and proliferation of silkworm microsporidium Nosema bombycis.

Background: As the first identified microsporidium, Nosema bombycis can infect silkworm Bombyx mori to cause pebrine which brings huge economic losses to sericulture all over the world. The life cycle of Nosema bombycis is divided into three phases, including the infective phase, the proliferative phase and the sporogonic phase. The factor(s) that regulate spore maturation of Nosema bombycis is still unknown.

The Function of Two β-Ketoacyl-CoA Synthases on the Fatty Acid Composition.

Rapeseed ( L.) is one of the four major oilseed crops in the world and is rich in fatty acids. Changes in the fatty acid composition affect the quality of rapeseed.

Epidemiological features of uterine fibroid-associated imaging changes in Chinese women of reproductive age: a retrospective study.

Objectives: To investigate uterine fibroid (UF)-associated imaging changes, and their prevalence, incidence and potential risk factors in the Chinese population.

Design: This was a retrospective observational study using health examination data.

Setting: A physical examination centre in Nanchong, China, between October 2017 and December 2020.

Role of N6-methyladenosine methylation in nasopharyngeal carcinoma: current insights and future prospective.

Nasopharyngeal carcinoma (NPC) is a distinct type of head and neck squamous cell carcinoma prevalent in Southern China, Southeast Asia, and North Africa. Despite advances in treatment options, the prognosis for advanced NPC remains poor, underscoring the urgent need to explore its underlying mechanisms and develop novel therapeutic strategies. Epigenetic alterations have been shown to play a key role in NPC progression.

Equine lentivirus Gag protein degrades mitochondrial antiviral signaling protein via the E3 ubiquitin ligase Smurf1.

Equine infectious anemia virus (EIAV) and HIV-1 are both members of the genus and are similar in virological characters. EIAV is of great concern in the equine industry. Lentiviruses establish a complex interaction with the host cell to counteract the antiviral responses.

Dual-localization signals enhance mitochondrial targeted presentation of engineered proteins.

Effective delivery of engineered proteins into mitochondria is of great significance for developing efficient mitochondrial DNA editing tools and realizing accurate treatment of mitochondrial diseases. Here, the candidate genes, and , were engineered with different mitochondrial localization signal (MLS) sequences introduced at their up- or/and down-streams. The corresponding expression vectors for the engineered proteins were constructed respectively, and HEK293T cells were transfected with these vectors.

ALYREF recruits ELAVL1 to promote colorectal tumorigenesis via facilitating RNA m5C recognition and nuclear export.

ALYREF can recognize 5-methylcytosine (m5C) decoration throughout RNAs to regulate RNA metabolism. However, its implications in cancer and precise regulatory mechanisms remain largely elusive. Here, we demonstrated that ALYREF supported colorectal cancer (CRC) growth and migration.

Yield, cell structure and physiological and biochemical characteristics of rapeseed under waterlogging stress.

Rapeseed (Brassica napus L.) is a major oilseed crop in the middle and lower reaches of the Yangtze River in China. However, it is susceptible to waterlogging stress.

alleviates sleep deprivation-induced intestinal barrier dysfunction and neuroinflammation in mice.

Consuming probiotic products is a solution that people are willing to choose to augment health. As a global health hazard, sleep deprivation (SD) can cause both physical and mental diseases. The present study investigated the protective effects of (), a widely used probiotic, on a SD mouse model.

Metabolomic and transcriptomic analyses highlight metabolic regulatory networks of Salvia miltiorrhiza in response to replant disease.

Background: Salvia miltiorrhiza, a well-known traditional Chinese medicine, frequently suffers from replant diseases that adversely affect its quality and yield. To elucidate S. miltiorrhiza's metabolic adaptations to replant disease, we analyzed its metabolome and transcriptome, comparing normal and replant diseased plants for the first time.