Label-free immunoassay based on chemiluminescence-functionalized magnetic mesoporous nanoparticles for rapid detection of neuron-specific enolase.

Lung cancer, as one of the cancers with the highest morbidity and mortality rates in the world, requires accurate detection of its vital serum marker, neuron-specific enolase (NSE), which is a key challenge for early detection of lung cancer. However, traditional chemiluminescence immunoassay (CLIA) methods rely on labeled antibodies (Abs) and suffer from complex operations and high costs. In this work, a label-free CLIA based on CL-functionalized mesoporous magnetic nanoparticles (CuFeO@mSiO-Cys-Luminol-Au NPs) is developed for the rapid and sensitive detection of NSE.

Saliva-derived exosomes regulate fibroblast metabolic reprogramming in skin wound healing.

Background: Effective skin repair requires rapid wound closure accompanied by precise extracellular matrix (ECM) remodeling and balanced cellular metabolism. Saliva-derived exosomes (S-Exo) have emerged as promising therapeutic agents due to their rich bioactive components; however, their mechanisms in ECM remodeling and metabolic regulation remain unclear. This study aimed to elucidate how S-Exo modulate ECM turnover through metabolic reprogramming, particularly glycolysis, in human skin fibroblasts (HSFs), and identify critical exosomal molecules mediating these effects.

Expert consensus on management of instrument separation in root canal therapy.

Instrument separation is a critical complication during root canal therapy, impacting treatment success and long-term tooth preservation. The etiology of instrument separation is multifactorial, involving the intricate anatomy of the root canal system, instrument-related factors, and instrumentation techniques. Instrument separation can hinder thorough cleaning, shaping, and obturation of the root canal, posing challenges to successful treatment outcomes.

Dentinogenesis imperfecta type II dentin: nanostructural mechanics analysis.

Objectives: This study investigated the nanomechanical properties, microstructure, and composition of dentinogenesis imperfecta type II (DGI-II) peritubular dentin (PTD) and intertubular dentin (ITD) and examined the correlations between them.

Materials And Methods: Six samples from each of the normal and DGI-II groups were prepared by cutting the midcoronal dentin perpendicular to the dentin tubules. The number and morphology of the dentin tubules were then observed by scanning electron microscopy (SEM).

Visualized chemiluminescence immunoassay for simultaneous detection of multiple lung cancer markers based on spatially resolved microfluidic paper chip device.

Lung cancer ranks as the most prevalent form of cancer. Early detection and diagnosis can lower death rates, and variations in tumor markers can help assess cancer risk. In this work, a glow-type chemiluminescent (CL) hydrogel SCG-Co-luminol was synthesized by a simple approach, and this hydrogel was integrated with a 3D microfluidic paper-based analytical device (3D μPAD) to develop a visual biosensor for identifying multiple lung cancer markers.

Association between or gene polymorphism and tooth agenesis risk: A meta-analysis.

Tooth loss represents the most prevalent form of dental agenesis. Anterior tooth loss primarily impacts aesthetics and psychological well-being, whereas posterior tooth loss influences bone growth patterns and masticatory function. Prolonged tooth loss can significantly hinder subsequent restorative procedures.

Assessment of Physical Properties, Obturation Quality, and Cytocompatibility/Osteogenic Potential of KP-Root SP: A New Calcium Silicate-based Bio-ceramic Sealer.

Introduction: The study aims to evaluate the physical properties, obturation quality, biocompatibility, and osteogenic induction characteristics of KP-Root SP with iRoot SP.

Methods: The evaluation of flow, film thickness, radiopacity, and solubility was conducted according to ISO 6876 Dentistry-Root Canal Sealing Materials. Forty isolated premolars, after preparation, were numbered and randomly assigned into 2 groups, and root-filled using size-fitted gutta-percha master cones along with KP-Root SP or iRoot SP sealers.

Visual Antimicrobial Gold Nanocluster Hydrogel with Inflammation-Responsive and Time-Regulated Swelling/Degradation for Regenerative Endodontic Procedures.

Regenerative endodontic procedures (REPs) have become the primary therapy for immature permanent teeth. Double antibiotic (DA, ciprofloxacin, and metronidazole) is recommended as the intracanal medicament in REPs. However, it has several limitations, such as toxicity to stem cells, short-term antibacterial efficacy, and inability to be visualized radiographically.

Expert consensus on intentional tooth replantation.

Intentional tooth replantation (ITR) is an advanced treatment modality and the procedure of last resort for preserving teeth with inaccessible endodontic or resorptive lesions. ITR is defined as the deliberate extraction of a tooth; evaluation of the root surface, endodontic manipulation, and repair; and placement of the tooth back into its original socket. Case reports, case series, cohort studies, and randomized controlled trials have demonstrated the efficacy of ITR in the retention of natural teeth that are untreatable or difficult to manage with root canal treatment or endodontic microsurgery.

Expert consensus on pulpotomy in the management of mature permanent teeth with pulpitis.

Pulpotomy, which belongs to vital pulp therapy, has become a strategy for managing pulpitis in recent decades. This minimally invasive treatment reflects the recognition of preserving healthy dental pulp and optimizing long-term patient-centered outcomes. Pulpotomy is categorized into partial pulpotomy (PP), the removal of a partial segment of the coronal pulp tissue, and full pulpotomy (FP), the removal of whole coronal pulp, which is followed by applying the biomaterials onto the remaining pulp tissue and ultimately restoring the tooth.

The clinical characteristics and surgical strategy in infants with lingual thyroglossal duct cyst.

Objectives: Lingual Thyroglossal Duct Cysts (LTDCs) are a rare variant of thyroglossal duct Cyst (LDC). This study aimed to explore the efficacy of transoral excision of LTDC and evaluate the added benefit of concomitant management of laryngomalacia during the surgical intervention.

Methods: Infants with LTDCs were retrospectively collected from our department from January 2009 to January 2022.

Expert consensus on apical microsurgery.

Apical microsurgery is accurate and minimally invasive, produces few complications, and has a success rate of more than 90%. However, due to the lack of awareness and understanding of apical microsurgery by dental general practitioners and even endodontists, many clinical problems remain to be overcome. The consensus has gathered well-known domestic experts to hold a series of special discussions and reached the consensus.

Genetic analysis of patients with low-frequency non-syndromic hearing loss.

Low-frequency non-syndromic hearing loss (LFNSHL) is a rare auditory disorder affecting frequencies ≤ 2000 Hz. To elucidate its genetic basis, we conducted whole-exome sequencing on nine Chinese families (31 affected individuals) with LFNSHL. Four heterozygous pathogenic variants, including two novel variants, were identified in common LFNSHL-related genes (WFS1, DIAPH1) and less common genes (TNC, EYA4), achieving a 44% genetic diagnosis rate.

CircRNA-mediated heterogeneous ceRNA regulation mechanism in periodontitis and peri-implantitis.

Background: Performing a comprehensive study on the differential expression of mRNAs, miRNAs, and circRNAs in the context of peri-implantitis and periodontitis has beneficial advantages to identify unique molecular signatures and pathways that may contribute to our understanding of these conditions.

Methods: Gingival tissues from healthy individuals and peri-implantitis and periodontitis patients were obtained to identify differential expression genes (DEG) by Illumina HiSeq 2500 instrument. Differential expression analysis was conducted using R statistical software, with significance set at P < 0.

RNAi Screen Identifies AXL Inhibition Combined with Cannabinoid WIN55212-2 as a Potential Strategy for Cancer Treatment.

: Cannabinoids are commonly used as adjuvant cancer drugs to overcome numerous adverse side effects for patients. The aim of this study was to identify the target genes that show a synergistic anti-tumor role in combination with the cannabinoid WIN55212-2 in vitro and in vivo. : A human kinome RNAi library was used to screen the targeted gene that silencing plus WIN55212-2 treatment synergistically inhibited cancer cell growth in an INCELL Analyzer 2000.

Efficacy and safety of spleen aminopeptide oral solution for children with allergic rhinitis and adenoid hypertrophy: a randomised trial.

Background: The spleen aminopeptide oral solution (SAOS) is a solution containing a mixture of polypeptide amino acids and polynucleotides derived from healthy bovine spleen. This study aimed to establish a randomized controlled clinical trial to evaluate the efficacy and safety of SAOS for children with allergic rhinitis (AR) and adenoid hypertrophy (AH).

Methods: Children with AR and AH who visited the Otolaryngology Department of the Children's Hospital Affiliated with Fudan University between June 2022 and April 2023 were included.

Integrated analysis of transcriptome and proteome reveals a core set of genes involved in osteoblast under oxidative stress.

Osteoblasts dysfunction, induced by oxidative stress (OS), is a significant contributor to the pathogenesis of osteoporosis. However, the genes implicated in regulating osteoblast dysfunction remain unclear. Here, we employed the hydrogen peroxide (HO)-induced osteoblast dysfunction model to assess its impact on osteoblast phenotype and to conduct transcriptome and proteome analyses in osteoblasts under OS.

Role of the PGAM5-CypD mitochondrial pathway in methylglyoxal-induced bone loss in diabetic osteoporosis.

Diabetic osteoporosis (DOP) is a skeletal complication with a high rate of disability. It results in a great burden to the patient's family and society. Methylglyoxal (MG) is a toxic by-product of the glycolytic process that occurs during diabetic conditions.

Integration of host gene regulation and oral microbiome reveals the influences of smoking during the development of oral squamous cell carcinoma.

Objective: This study aims to investigate the regulation of host gene transcription and microbial changes during the development of oral squamous cell carcinoma (OSCC) associated with smoking.

Methods: The OSCC mouse model and smoking mouse model were established using 200 μg/mL 4-nitroquinoline-1-oxide (4NQO) in drinking water and exposure to cigarette smoke (four cigarettes per session, once a day, 5 days a week). Tongue tissues were harvested at 4 weeks and 16 weeks.

MiR-143-5p regulates the proangiogenic potential of human dental pulp stem cells by targeting HIF-1α/RORA under hypoxia: A laboratory investigation in pulp regeneration.

Aim: Angiogenesis is a key event in the successful healing of pulp injuries, and hypoxia is the main stimulator of pulpal angiogenesis. In this study, we investigated the effect of hypoxia on the proangiogenic potential of human dental pulp stem cells (hDPSCs) and the role of miR-143-5p in the process.

Methodology: Human dental pulp stem cells were isolated, cultured and characterized in vitro.