Label-free immunoassay based on chemiluminescence-functionalized magnetic mesoporous nanoparticles for rapid detection of neuron-specific enolase.

Lung cancer, as one of the cancers with the highest morbidity and mortality rates in the world, requires accurate detection of its vital serum marker, neuron-specific enolase (NSE), which is a key challenge for early detection of lung cancer. However, traditional chemiluminescence immunoassay (CLIA) methods rely on labeled antibodies (Abs) and suffer from complex operations and high costs. In this work, a label-free CLIA based on CL-functionalized mesoporous magnetic nanoparticles (CuFeO@mSiO-Cys-Luminol-Au NPs) is developed for the rapid and sensitive detection of NSE.

Butyrate derived from intratumoral microbiome facilitates pulmonary metastasis in osteosarcoma.

Osteosarcoma, the predominant primary malignant bone tumor, has a dismal prognosis due to pulmonary metastasis, necessitating innovative strategies for early prediction and targeted therapy. While microbial metabolites such as butyrate are increasingly recognized as cancer modulators, their mechanistic involvement in osteosarcoma metastasis remains undefined. Here, we integrated multiomics integration of 16S rRNA sequencing and circulating microbial DNA (cmDNA) profiling to investigate intratumoral microbiome dynamics in osteosarcoma pulmonary metastasis.

Comparison of flipped classroom and lecture-based models in a radiology residency training program.

Background: The flipped classroom (FC) model is an effective teaching method that flips traditional teaching process dominated by teachers to students. Few studies have applied the FC model to the radiology residency training.

Objective: This study analyzed the learning ability, learning efficiency and satisfaction with teaching quality of an FC group of residents (n = 67) and compared the findings with those of a lecture-based (LB) teaching group (n = 60) at the Affiliated Hospital of Anhui Medical University.

Development of Orally Bioavailable FTO Inhibitors with Potent Antileukemia Efficacy.

N-Methyladenosine (mA), the most prevalent mRNA modification, undergoes dynamic regulation mediated by the demethylase fat mass and obesity-associated protein (FTO), which is aberrantly overexpressed in acute myeloid leukemia (AML) and drives leukemogenesis. Based on the structure-guided optimization of our previously reported FTO inhibitor and fluorescein, we developed , a tricyclic benzoic acid derivative with potent FTO inhibitory activity and improved pharmacokinetic properties. exerts a robust antiproliferative effect on AML cells by suppressing oncogenic FTO signaling.

Cross-Trait Cross-Genome Cross-Organ Analysis of Gastrointestinal Disorders and Depression.

Background: The aim of this study was to explore the shared genetic architecture between inflammatory bowel disease and depression and other upper gastrointestinal dysfunctions and inflammatory diseases, and to identify shared risk loci, potential key tissues, and associated genetic mechanisms to study.

Methods: Based on pooled data from a large-scale genome-wide association study (GWAS), we observed a genetic correlation between inflammatory bowel disease and depression and other upper gastrointestinal tract dysfunctions and inflammatory disorders and performed cross-trait pleiotropy analyses to detect shared pleiotropic loci and genes. In addition, we performed a series of functional annotation and tissue-specific analyses to determine the impact of pleiotropic genes.

lncRNA FENDRR Predicts Adverse Prognosis and Regulates the Development of Esophageal Squamous Cell Carcinoma Through Negatively Modulating miR-495-3p.

Background/Aims: Esophageal squamous cell carcinoma (ESCC) is a major subtype of esophageal carcinoma and is highly prevalent in China. Identification of effective biomarkers could benefit ESCC management and therefore improve clinical outcomes. Evaluating the expression and significance of long non-coding RNA Fetal-lethal non-coding developmental regulatory RNA (FENDRR) in ESCC aims to provide a biomarker candidate for ESCC.

Visualized chemiluminescence immunoassay for simultaneous detection of multiple lung cancer markers based on spatially resolved microfluidic paper chip device.

Lung cancer ranks as the most prevalent form of cancer. Early detection and diagnosis can lower death rates, and variations in tumor markers can help assess cancer risk. In this work, a glow-type chemiluminescent (CL) hydrogel SCG-Co-luminol was synthesized by a simple approach, and this hydrogel was integrated with a 3D microfluidic paper-based analytical device (3D μPAD) to develop a visual biosensor for identifying multiple lung cancer markers.

Bortezomib induces cell apoptosis and increases the efficacy of αPD-1 in BCR::ABL T315I mutation CML by targeting UBE2Q1.

The BCR:ABL T315I mutation presents a significant challenge in the current management of Chronic Myeloid Leukemia (CML), highlighting the need to identify novel targets and drugs. In our study, we observed the elevated expression of UBE2Q1 in KBM5-T315I cells compared to KBM5 cells, where it interacted with DDX3, regulating its ubiquitination. Furthermore, we found that Bortezomib (BTZ) targeted UBE2Q1, reducing its protein level expression.

Application of Dynamic [F]FDG PET/CT Multiparametric Imaging Leads to an Improved Differentiation of Benign and Malignant Lung Lesions.

Purpose: To evaluate the potential of whole-body dynamic (WBD) 2-deoxy-2-[F]fluoro-D-glucose positron emission tomography/computed tomography ([F]FDG PET/CT) multiparametric imaging in the differential diagnosis between benign and malignant lung lesions.

Procedures: We retrospectively analyzed WBD PET/CT scans from patients with lung lesions performed between April 2020 and March 2023. Multiparametric images including standardized uptake value (SUV), metabolic rate (MR) and distribution volume (DV) were visually interpreted and compared.

GL-V9 induce apoptosis of CML cells via MAPK signaling pathway.

GL-V9, a derivative of wogonin, has shown potent antitumor effects in various cancers, yet its impact on chronic myeloid leukemia (CML) remains unexplored. In this study, we found that GL-V9 significantly decreased the viability of CML cells. Annexin V/PI staining demonstrated that GL-V9 induced apoptosis in a concentration-dependent manner.

Self-Assemble Silk Fibroin Microcapsules for Cartilage Regeneration through Gene Delivery and Immune Regulation.

Effective treatments for cartilage defects are currently lacking. Gene delivery using proper delivery systems has shown great potential in cartilage regeneration. However, the inflammatory microenvironment generated by the defected cartilage severely affects the system's delivery efficiency.

Improving the upper atmospheric temperature accuracy of the ground-based instrument by eliminating noise ways.

In view of the special properties of the upper atmosphere at the altitude of 80-120 km, a ground-based passive remote sensing instrument ground-based airglow volume emission rate and temperature imaging interferometer (GBAVTII) is built to detect the atmospheric temperature used (0-1) spectral line of night airglow at the altitude of 94 km. In the process of photographing the upper atmosphere airglow with the GBAVTII, the stray light (white noise) such as moonlight, city lights, and starlight will be affected. In this paper, the theoretical expression of denoising is derived based on the rotational line temperature measurement of diatomic (0-1) airglow.

Value of whole-body dynamic F-FMISO PET/CT Patlak multi-parameter imaging for evaluating the early radiosensitizing effect of oleanolic acid on C6 rat gliomas.

The purpose of this study was to investigate the value of tumour-to-muscle (T/M) ratios and Patlak Ki images extracted from whole-body dynamic F-fluoromisonidazole (FMISO) PET/CT Patlak multi-parameter imaging for evaluating the early radiosensitizing effect of oleanolic acid (OA). Twenty-four rats with C6 gliomas were divided into 4 groups and treated with OA (group B), radiotherapy (group C), both (group D) or neither (group A). Whole-body dynamic F-FMISO PET/CT scans were performed for 120 min before treatment and 24 h following the treatment course.

Madecassic Acid Ameliorates the Progression of Osteoarthritis: An in vitro and in vivo Study.

Purpose: Osteoarthritis (OA) places a significant burden on society and finance, and there is presently no effective treatment besides late replacement surgery and symptomatic relief. The therapy of OA requires additional research. Madecassic acid (MA) is the first native triterpenoid compound extracted from Centella asiatica, which has a variety of anti-inflammatory effects.

A case report of old injury of lateral collateral ligament of knee joint combined with injury of common peroneal nerve.

Since common peroneal nerve is easy be injured because of superficial position of caput fibulae, less surrounding soft tissue and poor mobility, injury of common peroneal nerve is a problem worth discussing in the field of trauma orthopedics. Common peroneal nerve injury often causes foot prolapse, inability in dorsiflexion and eversion, sensory disturbance of anterolateral side of the lower leg and dorsum of foot. In this article, a case of old injury of lateral collateral ligament of knee joint combined with an avulsion fracture of fibular head resulting in injury of common peroneal nerve was reported and repaired by surgery with good effects.

F-FDG Micro PET/CT imaging to evaluate the effect of BRCA1 knockdown on MDA-MB231 breast cancer cell radiosensitivity.

Objective: Radioresistance of tumor cells is a major factor associated with failure of radiotherapy (RT). This study aimed to investigate the effect of BRCA1 knockdown on MDA-MB231 breast cancer cell radiosensitivity.

Materials And Methods: Short hairpin RNA (shRNA) was used to knockdown BRCA1 gene in MDA-MB231 cells.

Luminol functionalized tin dioxide nanoparticles with catalytic effect for sensitive detection of glucose and uric acid.

In this work, novel luminol functionalized nanoparticles (SnO@Luminol NPs) with mesoporous spherical structure were synthesized by a simple and green method, which exhibited good chemiluminescence (CL) intensity without any additional catalysts. In addition, the CL intensities generated by SnO@Luminol/HO system were linearly related to the logarithm values of HO concentrations, and CL mechanism of SnO@Luminol/HO system was investigated. On this basis, a sensitive CL method was proposed to determine glucose and uric acid.

Design, synthesis, and in vivo evaluation of GO-SWL-Ahx-K-SWL.

In order to acquire both expanded binding ability with the EphA2 receptor and superior drug delivery capacity, we designed and synthesized the modified GO-SWL-Ahx-K-SWL conjugate as a potential targeted therapeutic drug for non-small cell lung cancer (NSCLC). Various characterization methods have confirmed that the conjugate is consistent with the theoretical peptide. The cytotoxicity test results showed that the conjugate was slightly more toxic to A549 cells than in 3 T3 cells, and the toxicity increased in a concentration-dependent manner.

Oleanolic acid combined with olaparib enhances radiosensitization in triple negative breast cancer and hypoxia imaging with F-FETNIM micro PET/CT.

The heterogeneity of triple negative breast cancer (TNBC) results in the worst prognosis among breast cancer types, making its treatment strategy very challenging. A recent study showed that oleanolic acid (OA) has a radiosensitizing effect on tumor cells, but it does not show a good clinical effect when used alone in radiotherapy. The cytotoxicity of radiotherapy can be enhanced by modulating DNA repair, so new treatment options are being investigated to inhibit DNA repair pathways and sensitize tumors to radiation.

A novel polydopamine-modified metal organic frameworks catalyst with enhanced catalytic performance for efficient degradation of sulfamethoxazole in wastewater.

In this study, a novel polydopamine (PDA)-modified metal organic frameworks (MOFs) catalyst (MIL/PDA) was successfully fabricated to activate persulfate (PS) for the degradation of sulfamethoxazole (SMX) in wastewater. The experimental results indicated that PDA-modified catalyst exhibited superior catalytic performance and enhanced the degradation of SMX (91.5%) compared to pure MOFs.

Application of Intravoxel Incoherent Motion Diffusion-Weighted Imaging in Predicting and Monitoring Early Efficacy of Anti-Angiogenic Therapy in the C6 Glioma Rat Model.

Objective: To investigate the feasibility of intravoxel incoherent motion (IVIM) diffusion-weighted imaging (DWI) in evaluating early effects of anti-angiogenic therapy in the C6 glioma rat model.

Methods: Twenty-six rats of the C6 glioma model were randomly divided into a treatment group (received bevacizumab) and a control group (physiological saline). IVIM-DWI was performed on days 0, 1, 3, 5, and 7 after anti-angiogenic therapy and tumor growth and IVIM-DWI parameters were dynamically observed.

Electrocatalytic oxidation of ciprofloxacin by Co-Ce-Zr/γ-AlO three-dimensional particle electrode.

In this work, Co-Ce-Zr/γ-AlO3 particle electrodes were prepared for the efficient degradation of ciprofloxacin (CIP). Co-Ce-Zr/γ-AlO particle electrodes were analyzed with a scanning electron microscope (SEM), X-Ray Diffraction (XRD), X-Ray Fluorescence Spectrometer (XRF), X-ray photoelectron spectroscopy (XPS), and energy-dispersive X-ray spectroscopy (EDS). According to the results, significant amounts of CoO, CeO, and ZrO were formed on the Co-Ce-Zr/γ-AlO particle electrodes.

Characteristics of Very Low Frequency Sound Propagation in Full Waveguides of Shallow Water.

This work is concerned with the characteristics of very low frequency sound propagation (VLF, ≤100 Hz) in the shallow marine environment. Under these conditions, the classical hypothesis of considering the sea bottom as a fluid environment is no longer appropriate, and the sound propagation characteristics at the sea bottom should be also considered. Hence, based on the finite element method (FEM), and setting the sea bottom as an elastic medium, a proposed model which unifies the sea water and sea bottom is established, and the propagation characteristics in full waveguides of shallow water can be synchronously discussed.