Identification of pathogenic bacteria in saffron (Crocus sativus L.) by high-throughput sequencing and the evaluation of the antifungal properties of different plant essential oils.

Background: Saffron (Crocus sativus L.), a Chinese herbal medicine of significant medicinal value, is extensively cultivated worldwide. However, fungal pathogen-induced corm rot disease poses a major challenge to saffron cultivation, causing substantial economic losses and long-standing concern among researchers and industry professionals.

The Age-Male-Albumin-Bilirubin-Platelet Risk Score Predicts Postoperative Prognosis of Intrahepatic Cholangiocarcinoma: A Multicenter Retrospective Study.

Introduction: The age-male-albumin-bilirubin-platelets (AMAP) score serves as a specific model for assessing liver fibrosis among individuals with chronic hepatitis B. However, there remains ambiguity regarding its ability to reliably predict the long-term outcomes for patients diagnosed with intrahepatic cholangiocarcinoma (ICC) after undergoing radical surgical interventions.

Methods: This retrospective multicenter investigation included 681 participants diagnosed with ICC who had undergone radical surgical procedures.

Postoperative adjuvant hepatic arterial infusion chemotherapy with gemcitabine-cisplatin sequential capecitabine combined with PDL1 inhibitors in resected high-risk intrahepatic cholangiocarcinom: study protocol for a prospective, multicenter, single-arm, phase 2 trial (HgcCP trial).

Background: Although capecitabine is recommended for postoperative adjuvant treatment of biliary tract cancers (BTC), no studies have specifically focused on the postoperative adjuvant treatment of intrahepatic cholangiocarcinoma (ICC). In recent years, the combination of PD-L1 inhibitors and gemcitabine-cisplatin (GC) has demonstrated promising results in advanced BTC. The combination of GC, PD-L1 inhibitors, and capecitabine may be a potential adjuvant treatment for ICC.

Advances in AI-assisted quantification of dry eye indicators.

Dry eye disease (DED) is a multifactorial ocular surface disorder characterized by ocular discomfort, visual disturbances, and potential structural damage. The heterogeneous etiology and symptomatology of DED pose significant challenges for accurate diagnosis and effective treatment. In recent years, artificial intelligence (AI), particularly deep learning (DL), has shown substantial promise in improving the objectivity and efficiency of DED assessment.

Mobile genetic elements mediating antimicrobial resistance drive the evolutionary process of Clostridioides difficile ST37/RT017.

Background: () ST37/RT017 is one of the most prevalent genotypes, exhibiting resistance to multiple antimicrobial agents and widespread dissemination, particularly in East Asia. However, its evolutionary history and genetic adaptation remains limited. Here, we aimed to systematically assess the genetic diversity, key evolutionary events, and potential driving forces of ST37/RT017.

Association between carotenoids and weight-adjusted waist index a cross-sectional study based on the national health and nutrition examination survey 2017-2018.

Purpose: The weight-adjusted waist index (WWI) is a novel method for assessing abdominal obesity, offering greater accuracy than do body mass index and waist circumference in determining lean and fat mass. Carotenoids, known for their antioxidant properties, can effectively neutralize free radicals. We aimed to examine the relationship between serum carotenoid levels and WWI.

Intra-Arterial Tenecteplase After Successful Reperfusion in Large Vessel Occlusion Stroke: A Randomized Clinical Trial.

Importance: The optimal dose, safety, and efficacy of intra-arterial tenecteplase after successful reperfusion by endovascular thrombectomy for large vessel occlusion (LVO) is unknown.

Objective: To evaluate the dose-dependent adverse events and signals of efficacy of intra-arterial tenecteplase in LVO after successful reperfusion with thrombectomy, defined as an Extended Treatment in Cerebral Infarction score of 2b-3.

Design, Setting, And Participants: This open-label, blinded-outcome assessment trial, incorporating a 14 + 8 dose-escalation (phase 1b, nonrandomized) and dose-expansion (phase 2a, randomized) design, was conducted in China between 2023 and 2024, with follow-up continuing through November 2024.

Framework Nucleic Acid-Mediated DNAzyme Chaperoning for Sensitive Trace Metal Ion Mapping on Live Neuronal Cell Membranes.

The transient release of metal ions at neuronal cell membranes holds significant implications in neurophysiology and pathology. Consequently, there is a pressing need for sensitive methodologies capable of quantitatively mapping local metal ion concentrations in live cells, aiming to unravel potential mechanisms underlying neurological disorders. DNAzymes have emerged as versatile tools for metal ion detection in live cells, owing to their specificity and other inherent advantages.

A prospective longitudinal cohort study for quality of life in patients undergoing fourth level liver surgery.

Performing fourth-level liver surgery is an exceptionally demanding procedure, fraught with significant risks and technical complexities. Patients undergoing such operations face elevated surgical hazards, substantial physical trauma, prolonged recovery periods, and a multitude of factors that can profoundly impact their postoperative quality of life (QoL). Despite these challenges, there remains a notable gap in the literature regarding the long-term QoL outcomes for these individuals.

Pressure-Induced Structural Phase Transition in HoCeO Oxide.

The structural evolution of HoCeO under high pressure was systematically investigated using synchrotron X-ray diffraction (up to 31.5 GPa) and Raman spectroscopy (up to 41.7 GPa).

CD47-mediated tumor microenvironment remodeling: a central mechanism in immune evasion.

Immune evasion is a crucial strategy for tumor growth and survival, with the tumor microenvironment facilitating tumor immune evasion and cancer progression. CD47, a transmembrane protein highly expressed in various cancer cell types, interacts with its ligands SIRPα and TSP-1 to induce immune tolerance, enabling tumor cells to evade immune surveillance and phagocytosis by immune cells. Understanding the pathways driving CD47 signaling and related activation factors is essential.

Correction: ADHD and family life: A cross-sectional study of ADHD prevalence among pupils in China and factors associated with parental depression.

[This corrects the article DOI: 10.1371/journal.pone.

Studying the Coding Profiles of Somatic Stimulation on Cardiac-locked Neuronal Responses in the Rat Spinal Dorsal Horn.

Many studies have suggested that electroacupuncture may be beneficial in the treatment and prevention of cardiovascular disease. However, its mechanism remains poorly understood. The thoracic spinal dorsal horn (SDH) plays an important role in integrating and modulating somatic and visceral inputs, which may then influence cardiac control.

Ultra-low-dose Quantitative CT for Lumbar Bone Mineral Density Assessment: A Prospective Validation of Radiation Reduction and Diagnostic Accuracy.

Accurate assessment of bone mineral density (BMD) is essential for diagnosing osteoporosis, but conventional quantitative CT protocols are associated with relatively high radiation doses. This study aimed to evaluate the feasibility of a novel dual-parameter optimized ultra-low-dose CT (ULD-CT) protocol for quantitative BMD assessment in the lumbar spine, comparing its radiation dose, image quality, and diagnostic performance against standard-dose CT (SD-CT). In this prospective study, 245 patients undergoing lumbar surgery received paired pre- and postoperative CT scans: SD-CT (120kV/250mAs) and ULD-CT (100kV/30mAs).

Influence of portal hypertension-associated upper gastrointestinal bleeding and acute kidney injury on liver transplantation prognosis.

Background: Given that prioritization for liver transplantation (LT) is based primarily on the model for end-stage liver disease (MELD) scores, patients with lower MELD scores, who experience chronic anemia and recurrent hypotension due to gastrointestinal bleeding (GIB), tend to be marginalized.

Methods: A total of 581 patients with no evidence of acute kidney injury (AKI) or chronic kidney disease 2 months before LT constituted a retrospective cohort. Within this cohort, a nested case-control study was conducted that included 134 patients with preoperative GIB (GIB(+)) and 246 matched patients without preoperative GIB (GIB(-)).

Hemodynamic Impact of Cipepofol vs Propofol During Anesthesia Induction in Patients With Severe Aortic Stenosis: A Randomized Clinical Trial.

Importance: Postinduction hemodynamic instability is a frequent complication among patients with severe aortic stenosis (AS). Using cipepofol as the anesthesia agent may reduce the incidence and severity of hemodynamic instability.

Objective: To assess whether cipepofol outperforms propofol in maintaining postinduction hemodynamic stability in patients with AS.

Multiomics Studies on the Effects of High-Temperature Stress on Male Sterility in .

High-temperature (HT) stress has been recognized as one of the main factors restricting the normal growth and development of cotton and severely affects fiber quality and yield. To elucidate the regulatory mechanism of male sterility-related hormones in under HT stress, we explored candidate genes closely related to male sterility in . We studied the expression profiles of hormones and genes in the anthers of GB150 under HT stress by combining transcriptomic and metabolomic analyses.

Risk factors and prognosis of silent cerebral infarction after transcatheter aortic valve replacement.

The Valve Academic Research Consortium (VARC)-3 definition of silent cerebral infarction among neurologic events after transcatheter aortic valve replacement (TAVR) lacks clinical validation, yet its impact on postoperative in-hospital outcomes and long-term prognosis remains uncertain. This study aims to explore the perioperative related factors influencing the risk of SCI post-TAVR as defined by VARC-3 criteria, so as to identify high-risk individuals early and assess the effect of SCI on patient outcomes and one-year mortality following TAVR. This was a single-center study including 613 patients with severe aortic stenosis undergoing TAVR, with all data collected prospectively in a dedicated database.

Ferroptosis targeting offers a therapeutic target for septic cardiomyopathy.

Sepsis-induced cardiac dysfunction, usually termed sepsis-induced cardiomyopathy or septic cardiomyopathy(SCM), is developed in approximately 70 % of the patients with sepsis, making it is a major concern for sepsis patients. However, the pathogenesis of SCM remain incompletely understood. Ferroptosis, a newly identified mechanism of regulated cell death, characterized by a decline in antioxidant capacity, iron accumulation, and lipid peroxidation(LPO), is involved in sepsis and SCM.

Sirt5-mediated polarization and metabolic reprogramming of macrophage sustain brain function following ischemic stroke.

Ischemic stroke has become the leading cause of morbidity and mortality in adults. Reperfusion may initiate inflammatory response and cause damage to brain. Macrophage is supposed to be the major contributor of neuroinflammation and immune response.

Synthesis and Characterization of a Series of Biofriendly Fluoroether Betaines.

Fluoroether approach has been extensively employed as an alternative to conventional fluorinated surfactants; however, it faces challenges such as inadequate performance of short fluoroether chains and environmental contamination from long fluoroether chains. This study aims to address the conflict between environmental compatibility and the performance of fluoroether surfactants. The design of the surfactants is centered around the OC chain (CF(OCF)-), utilizing reduced fluorine content to achieve superior surface activity.

Traceability of septic shock caused by phocaeicola vulgatus: a rare case report.

Background: Phocaeicola vulgatus (P.vulgatus) is a member of Bacteroides fragilis Group(BFG). Septic shock caused by P.

Unveiling radiobiological traits and therapeutic responses of BRAF-mutant colorectal cancer via patient-derived organoids.

Background: Radiotherapy (RT) is an essential treatment for colorectal cancer (CRC), yet the factors influencing radiosensitivity remain unclear. In the quest to enhance the therapeutic efficacy in CRC, the interplay between genetic mutations and RT sensitivity has emerged as a pivotal yet enigmatic area.

Methods: We harness the fidelity of patient-derived organoids (PDOs) to dissect the molecular landscape of radiosensitivity, with a particular emphasis on BRAF mutations.

RBM39 promotes hepatocarcinogenesis by regulating RFX1's alternative splicing and subsequent activation of integrin signaling pathway.

Alternative splicing (AS) is crucial for tumor cells as it regulates protein expression and produces various protein isoforms, which can have diverse or even opposing roles in tumor growth and metastasis. Despite its significance, the role of AS and related splicing factors, particularly splicing-related messenger ribonucleoproteins (mRNPs), in hepatocarcinogenesis, is poorly understood. High-throughput transcriptome sequencing of HCC patients revealed that the spliceosome pathway might play a significant role in HCC development.

PTP-3 regulated by VB12 is important for ageing health in C. elegans.

Ageing is associated with cognitive decline, which is a significant factor in the development of dementia. Vitamin B12 (VB12) is crucial for maintaining proper nervous system function, as well as for protein, fat, and carbohydrate metabolism, and DNA synthesis. Moreover, it helps prevent serious health conditions such as pernicious anemia, neurodegenerative diseases, and Alzheimer's disease.