Application and efficacy of a self-made minimally invasive transverse cross-link in percutaneous pedicle screw surgery.

To design a minimally invasive transverse cross-link for use in percutaneous pedicle screw fixation surgery and to explore and observe its clinical application value. A retrospective analysis of the clinical data of 50 patients with thoracolumbar burst fractures treated with percutaneous pedicle screws was conducted. A self-made, minimally invasive transverse cross-link was implanted during surgery.

Investigation on the Interfacial Delamination of Glass Substrate Packaging Using Cohesive Zone Models.

This study aligns with the development trend of glass substrate packaging. The research aims to analyze the delamination of the substrate-adhesive layer-chip trilayer structure in packaging through experimental testing to obtain interface strength parameters. Subsequently, an iterative process combining experiments and simulations was applied to establish a cohesive zone model characterizing crack initiation and propagation.

Copper-overload promotes ferroptosis in cervical cancer cells by upregulating HMOX1 expression.

Cuproptosis is a newly defined regulated cell death model and is considered as a potential approach for cancer treatment. We have previously found that cervical cancer cells have the capability of anti-ferroptosis. A recent study has reported that elesclomol (ES) is able to induce copper-dependent ferroptosis.

Analysis of healing status and influencing factors of iatrogenic atrial septal defect in patients with atrial fibrillation after cryoballoon ablation.

Background: Iatrogenic atrial septal defect (IASD) may occur after cryoballoon ablation (CBA) for atrial fibrillation (AF) patients. This study aimed to investigate the incidence and healing status of IASD after CBA, and to analyze the risk factors and clinical significance associated with the occurrence of IASD.

Methods And Results: Patients with non-valvular AF who underwent CBA in Fujian Provincial Hospital from 2021 to 2023 were continuously enrolled.

Biomechanical effects of transforaminal endoscopic lumbar discectomy combined with spinal dynamic stabilization system use on adjacent segments: a finite element analysis.

Objective: To assess the biomechanical effect of transforaminal endoscopic lumbar discectomy combined with spinal dynamic stabilization system (TELD-SDSS) on the adjacent segments using three-dimensional finite element (3D-FE) analysis, providing reference data for the optimization of surgical approaches for giant lumbar disc herniation (GLDH).

Methods: A 3D-FE model of L3-S1 was constructed based on imaging data and validated against well-established in-vitro biomechanical data. Surgical FE models representing TELD, TELD-SDSS, and posterior lumbar interbody fusion (PLIF) were reconstructed.

Ultra-low-dose Quantitative CT for Lumbar Bone Mineral Density Assessment: A Prospective Validation of Radiation Reduction and Diagnostic Accuracy.

Accurate assessment of bone mineral density (BMD) is essential for diagnosing osteoporosis, but conventional quantitative CT protocols are associated with relatively high radiation doses. This study aimed to evaluate the feasibility of a novel dual-parameter optimized ultra-low-dose CT (ULD-CT) protocol for quantitative BMD assessment in the lumbar spine, comparing its radiation dose, image quality, and diagnostic performance against standard-dose CT (SD-CT). In this prospective study, 245 patients undergoing lumbar surgery received paired pre- and postoperative CT scans: SD-CT (120kV/250mAs) and ULD-CT (100kV/30mAs).

[Effect of the sequence of intermediate instrumentation and distraction-reduction of the injured vertebra on the treatment of thoracolumbar burst fractures with high rate of spinal canal encroachment].

Objective: To investigate the effect of the sequence of intermediate instrumentation and distraction-reduction of the injured vertebra on the surgical efficacy of short-segment percutaneous pedicle screw fixation for thoracolumbar burst fractures with high rate of spinal canal encroachment.

Methods: From January 2016 to January 2022, 38 patients with thoracolumbar burst fractures with high rate of spinal canal encroachment (spinal canal encroachment rate >40%, complete posterior longitudinal ligament, no flipping bone block in the posterior marginal of the vertebra) without spinal cord injury who were were treated with short-segment percutaneous pedicle screw fixation were retrospectively analyzed. During the operation, 18 cases were used distraction-reduction first and then intermediate instrumentation on injured vertebral and sequential distraction-reduction again(the distraction-reduction first group) including 8 females and 10 males with a mean age of 46.

Cardiac FGF23 Increases Intracellular Calcium in Atrial Myocytes and the Susceptibility to Atrial Fibrillation Decreased in FGF23 MyHC Mice.

Exogenous fibroblast growth factor (FGF) 23 is closely associated with atrial fibrillation (AF) and is able to alter the cardiac electrophysiological activity by increasing intracellular calcium. While its arrhythmogenic mechanism remains unclear, this study aims to investigate the electrophysiological effects of cardiac FGF23 on intracellular calcium in atrial cells and its underlying mechanism. The incidence of AF was significantly decreased in FGF23MyHC mice compared to Cre mice.

PLIN2: a potential prognostic markers of early-stage atypical endometrial hyperplasia.

Objective: In the background of endometrial hyperplasia triggered by obesity and estrogen, could the perilipin 2 (PLIN2) serve as a possible prognostic marker for early atypical endometrial hyperplasia (AEH)?

Methods: A retrospective study examined blood lipid levels in endometrial cancer (EC) or AEH patients. An AEH mice model was established administrating with estradiol and/or high-fat (HF) diet. Hematoxylin and eosin staining were employed to assess pathological changes in the endometrium.

Pharmacologically targeting fatty acid synthase-mediated de novo lipogenesis alleviates osteolytic bone loss by directly inhibiting osteoclastogenesis through suppression of STAT3 palmitoylation and ROS signaling.

Aberrant increases in osteoclast formation and/or activity are the underlying cause of bone loss in a variety of osteolytic diseases. Fatty acid synthase (Fasn)-mediated de novo lipogenesis (DNL) is one of the major lipid metabolic pathways and has been shown to play critical roles in diverse physiological and pathological processes. However, little is known about its role in osteoclastogenesis.

Post-evaluation model for 500 kV substation engineering projects using the fuzzy comprehensive evaluation method.

To accurately assess the economic benefits and expected outcomes of investing in a 500 kV power transmission and substation project, it is essential to conduct a comprehensive economic evaluation of the entire system. This paper develops a post-evaluation model based on the fuzzy comprehensive evaluation (FCE) method tailored for 500 kV transmission and substation projects. Using this model, the implementation process is thoroughly evaluated.

A combined proteomic and metabolomic analysis of the early aborted embryonic tissues with maternal COVID-19 infection.

COVID-19 still spreads worldwide, and repeated infections are hard to avoid. Maternal infection during pregnancy is associated with adverse maternal and neonatal outcomes. Our study used a multi-omics profiling method to explore the proteome and metabolome alteration in early embryonic development after COVID-19 infection.

Vascular Cell Adhesion Molecule 1 in atrial fibrillation.

Objective: Assessing whether serum Vascular Cell Adhesion Molecule 1(VCAM-1) concentration in the left atrial appendage(LAA) and the expression of VCAM-1 in LAA tissues are associated with the risk of atrial fibrillation-related stroke.

Method: Blood samples were collected from atrial fibrillation(AF) patients scheduled for catheter ablation of AF, both from within the LAA and peripheral veins(PV). The serum concentration of VCAM-1 was quantitatively analyzed using ELISA.

Concurrent assessment of time-to-isolation and temperature at 30 s as an innovative metric for predicting the persistence of pulmonary vein isolation using second-generation cryoballoons.

Background: We attempted to establish correlations between intraoperative variables such as time-to-isolation (TTI) and temperature (T) at the 30-second mark, and the sustained efficacy of pulmonary vein isolation.

Methods: One hundred patients underwent repeat procedures subsequent to their index ablation. Five time intervals were delineated based on TTI metrics of 30, 35, 40, 45, and 60 s during the initial procedure.

Three-dimensional modeling of 500 kV transmission lines by airborne LiDAR.

This study aims to enhance the accuracy of three-dimensional (3D) modeling in the planning, design, operation management, and risk assessment of 500 kV transmission lines. Addressing the limitations of traditional two-dimensional (2D) or low-precision 3D models, a new method based on airborne Light Detection and Ranging (LiDAR) technology is proposed. Initially, high-density, high-precision 3D point cloud data are collected using airborne LiDAR technology.

Calcium-mediated DAD in membrane potentials and triggered activity in atrial myocytes of ETV1 MyHC mice.

The E-twenty-six variant 1 (ETV1)-dependent transcriptome plays an important role in atrial electrical and structural remodelling and the occurrence of atrial fibrillation (AF), but the underlying mechanism of ETV1 in AF is unclear. In this study, cardiomyocyte-specific ETV1 knockout (ETV1MyHC, ETV1-CKO) mice were constructed to observe the susceptibility to AF and the underlying mechanism in AF associated with ETV1-CKO mice. AF susceptibility was examined by intraesophageal burst pacing, induction of AF was increased obviously in ETV1-CKO mice than WT mice.

Harnessing microbial antigens as cancer antigens: a promising avenue for cancer immunotherapy.

Immunotherapy has revolutionized cancer treatment by leveraging the immune system's innate capabilities to combat malignancies. Despite the promise of tumor antigens in stimulating anti-tumor immune responses, their clinical utility is hampered by limitations in eliciting robust and durable immune reactions, exacerbated by tumor heterogeneity and immune evasion mechanisms. Recent insights into the immunogenic properties of host homologous microbial antigens have sparked interest in their potential for augmenting anti-tumor immunity while minimizing off-target effects.

Maternal androgen exposure induces intergenerational effects via paternal inheritance.

Polycystic ovary syndrome (PCOS) is a condition resulting from the interaction between environmental factors and hereditary components, profoundly affecting offspring development. Although the etiology of this disease remains unclear, aberrant in utero androgen exposure is considered one of the pivotal pathogenic factors. Herein, we demonstrate the intergenerational inheritance of PCOS-like phenotypes in F2 female offspring through F1 males caused by maternal testosterone exposure in F0 mice.

Oxysterol 25-hydroxycholesterol activation of ferritinophagy inhibits the development of squamous intraepithelial lesion of cervix in HPV-positive patients.

Squamous intraepithelial lesion of cervix (SIL) in human papillomavirus (HPV)-positive patient often undergoes a silent and long-course development, and most of them with high-grade transit to cervical squamous cell carcinoma (CSCC). The oxysterol 25-hydroxycholesterol (25-HC) is associated with HPV inhibition, autophagy and cholesterol synthesis, however, its function in this long process of SIL development remain unclear. In this study, we demonstrate that 25-HC generation is inhibited through HSIL-to-CSCC transition.

Piperlongumine, a -derived amide alkaloid, protects mice from ovariectomy-induced osteoporosis by inhibiting osteoclastogenesis suppression of p38 and JNK signaling.

Postmenopausal osteoporosis (PMOP) is a metabolic bone disease that results from overproduction and hyperactivation of osteoclasts caused by insufficient estrogen in women after menopause. Current therapeutic strategies are mainly focused on treating PMOP patients who have already developed severe bone loss or even osteoporotic fractures. Obviously, a better strategy is to prevent PMOP from occurring in the first place.

Intervertebral disc-intrinsic Hedgehog signaling maintains disc cell phenotypes and prevents disc degeneration through both cell autonomous and non-autonomous mechanisms.

Intervertebral disc degeneration is closely related to abnormal phenotypic changes in disc cells. However, the mechanism by which disc cell phenotypes are maintained remains poorly understood. Here, Hedgehog-responsive cells were found to be specifically localized in the inner annulus fibrosus and cartilaginous endplate of postnatal discs, likely activated by Indian Hedgehog.

Gaucher disease in a patient with membranoproliferative glomerulonephritis: case report.

Background: Gaucher disease (GD) is a rare autosomal recessive inherited, lysosomal storage disoder that involves liver, spleen, lung, bone, bone marrow even central nervous. However, GD associated membranoproliferative glomerulonephritis (MPGN) is seldom reported.

Case Presentation: Here we described a case of 35-year-old man suffering from GD with hepatosplenomegaly, ascites, bone destruction, myelofibrosis and MPGN.