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Article Abstract
In this work, novel luminol functionalized nanoparticles (SnO@Luminol NPs) with mesoporous spherical structure were synthesized by a simple and green method, which exhibited good chemiluminescence (CL) intensity without any additional catalysts. In addition, the CL intensities generated by SnO@Luminol/HO system were linearly related to the logarithm values of HO concentrations, and CL mechanism of SnO@Luminol/HO system was investigated. On this basis, a sensitive CL method was proposed to determine glucose and uric acid. The fabricated CL method displayed wide linear ranges (glucose, 1.0 × 10 - 1.0 × 10 M; uric acid, 1.0 × 10 - 1.0 × 10 M) and low detection limits (glucose, 5.4 nM; uric acid, 6.8 nM). The proposed CL method achieved sensitive and specific glucose and uric acid detection in human serum specimens, and the measured results were consistent with the clinical diagnosis. Furthermore, as long as the product of reaction between enzyme and substrate is HO, this CL method can also be extended to other biological detection fields.
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| http://dx.doi.org/10.1016/j.aca.2022.340070 | DOI Listing |
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