A Versatile DNAzyme-Amplified Protease-Sensing Platform for Accurate Diagnosis of SARS-CoV-2 and Reliable Classification of Colorectal Cancer.

Peptide-based biosensors are widely used for in vitro detection of protease activity but often suffer from the limited sensitivity, poor accuracy, and incompatibility with point-of-care testing (POCT) devices. Herein, we developed a versatile deoxyribozyme (DNAzyme)-amplified protease-sensing (DP) platform that integrates the positively charged oligopeptides with a negatively charged DNAzyme biocatalyst for highly-sensitive protease detection. The system leverages the electrostatic peptide-DNAzyme interactions to inhibit DNAzyme catalytic activity, which is reactivated upon the protease-triggered peptide hydrolysis, thus enabling an efficient signal amplification via the successive cleavage of DNAzyme substrate.

Identifying determinants of viral hepatitis and liver cancer care in Michigan Asian American communities through multilevel engagement.

Background: In Michigan, Asian Americans are disproportionately infected with HBV and HCV. As many infections are first diagnosed when patients present with advanced liver disease or liver cancer, HBV and HCV screening, awareness, and early treatment are critical to improving outcomes.

Methods: Using a theory-informed approach, we administered a bi-level qualitative study to identify determinants of viral hepatitis and liver cancer care and treatment in Michigan Asian American communities.

Global burden and risk factors of type 2 diabetes mellitus from 1990 to 2021, with forecasts to 2050.

Background: Type 2 diabetes mellitus (T2DM) presents a substantial strain on global healthcare systems. This study seeks to offer robust scientific evidence for the effective prevention and management of T2DM globally through a comprehensive analysis of the disease's burden, trends, and risk factors from 1990 to 2021, as well as future trajectories from 2022 to 2050.

Methods: Data for this study were sourced from the 2021 Global Burden of Disease (GBD) study.

Leukocyte Count Is Better than LDL-C as Predictor of Novel Carotid Atherosclerosis.

this retrospective cohort study aimed to identify risk factors and establish cutoff values for the initial development of carotid atherosclerosis (CAS) in middle-aged adults. from an initial cohort of 3583 participants, we finally analyzed 1141 individuals. The observation group comprised subjects who developed CAS without concomitant fatty liver disease (FLD), using their last normal clinical measurements as predictors.

Electroacupuncture at Huantiao (GB30) and Zusanli (ST36) acupoints promotes peripheral nerve regeneration by enhancing energy metabolism in mice.

Background: Peripheral nerve injury (PNI) leads to substantial functional impairment, yet current therapies remain limited. Electroacupuncture (EA) is a promising intervention for PNI, but its mechanisms, particularly its role in modulating energy metabolism during nerve regeneration, are poorly understood.

Methods: A mouse PNI model was established by crushing the right sciatic nerve.

eHealth Literacy Interventions: Scoping Review.

Background: Electronic resources have become a predominant modality for health information dissemination in recent years. eHealth literacy (eHL) means individuals' competencies to effectively acquire and use health information from electronic sources. Enhancing eHL is thus essential to facilitate individuals' effective engagement with electronic resources and promote improved health management.

Unraveling the role of miRNAs in placental function: insights into trophoblast biology and pregnancy pathology.

MicroRNAs (miRNAs) are pivotal regulators of placental development and function, orchestrating critical processes such as trophoblast proliferation, differentiation, invasion, and vascular remodeling. By modulating diverse signaling pathways, these small non-coding RNAs help ensure proper placental formation and optimal maternal-fetal nutrients and gases exchange. Dysregulated miRNA expressions can profoundly affect placental biology, leading to complications such as preeclampsia, fetal growth restriction, and recurrent pregnancy loss.

Near-Infrared Light-Responsive Lubricating Coating with Polyaniline Microspheres for Long-Term Anti-Icing and High-Efficiency De-Icing.

Despite significant advances in anti-icing/de-icing coating in recent years, key issues such as lubricant leakage and high de-icing energy consumption still substantially restrict their long-term performance in cold environments. To address this, a long-lasting lubricating coating based on silicone oil-loaded polyaniline (PANI) microspheres is developed. Through innovative structural design and regulation, PANI microspheres achieved "three birds with one stone" effect: photothermal conversion, electrothermal conversion, and lubricating properties.

An LF-NMR Biosensor for the Detection of Circulating Tumor Cells Based on Target-Triggered Bioorthogonal Chemistry and Click Chemistry.

Circulating tumor cells (CTCs), crucial biomarkers for monitoring cancer metastasis and prognosis, present significant challenges for detection technologies due to their extremely low abundance in peripheral blood. This study introduces a low-field nuclear magnetic resonance (LF-NMR) biosensor leveraging the synergistic effect of biorthogonal chemistry and click chemistry for CTC detection by a sol-gel phase-transition strategy. Initially, CTCs were targeted and enriched using mucin 1-specific aptamers.

A novel framework for trajectory planning in robotic arm developed by integrating dynamical movement primitives with particle swarm optimization.

In human-robot collaboration, imitation learning and autonomous adaptation to new scenarios are pivotal concerns for robotic arms. To address these challenges, a novel framework (DMP-PSO) for trajectories planning in robotic arm is presented by integrating dynamical movement primitives (DMP) with particle swarm optimization (PSO) in this paper. Firstly, DMP is employed to learn and generalize motion trajectories.

Integrated photoelectrochemical/photothermal dual-mode biosensor with simultaneous dual-signal output for detection of bladder cancer cells HT-1376.

Here, a photoelectrochemical (PEC)/photothermal (PT) dual-mode biosensing platform with simultaneous output of response signals was developed for sensitive and accurate detection of bladder tumor cells HT-1376. The system utilized an infrared laser as a shared light source, with photocurrent and temperature measurements acquired through parallel PEC and PT detection channels. The HT-1376-specific aptamer (Apt) was covalently immobilized on aminated indium‑tin oxide (ITO) electrode via glutaraldehyde crosslinking between surface amino groups and amine-modified aptamer terminals, enabling selective target capture.

Evaluating the effectiveness of intelligent interaction technology in autism interventions: A meta-analysis based on trial assessment.

Purpose: This research aims to conduct a systematic review of the literature on the utilization of intelligent interaction technologies, including Extended Reality (XR) and robotic systems, within the context of autism interventions.

Method: This study commenced on June 16, 2022, and conducted a systematic search of publications from 2017 to 2024 using Boolean terms such as "Virtual Reality" AND "autism" AND "RCT" in PubMed, Web of Science, Cochrane Library, SpringerLink, and Embase. Two reviewers independently conducted research selection, data extraction, and quality assessment (using Cochrane Risk of Bias tool, RoB 2), and resolved differences through discussion.

NMDA Receptors Coordinate Metabolic Reprogramming and Mitophagy in Schwann Cells to Promote Peripheral Nerve Regeneration.

Schwann cells (SCs) are indispensable for peripheral nerve regeneration, yet the mechanisms enabling their metabolic adaptation to meet the energetic demands of axonal repair remain elusive. Here, we identify -methyl-D-aspartate (NMDA) receptors as central regulators of SC metabolic plasticity. In a mouse model of acute motor axonal neuropathy, nerve injury led to a marked decrease in NMDA receptor expression on SC.

METTL14 Promotes HK-2 Cell Injury in Diabetic Nephropathy Through the Regulation of THBS2 Expression in an m6A-YTHDF2-Dependent Manner.

To explore how methyltransferase like 14 (METTL14) regulates diabetic nephropathy (DN)-induced cell damage and analyze its underlying mechanisms. The gene expression data were obtained from the Gene Expression Omnibus (GEO) database and analyzed for differential expression using the DESeq2 package. The gene expression levels were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting.

Pangolin-type fluorescence imaging of circulating tumor cell based on functionalized 2D nanomaterial.

The isolation and detection of circulating tumor cell (CTC) is of great significance for clinical cancer diagnosis and personalized therapy. Herein, a novel pangolin-type fluorescence imaging strategy is proposed to recognize, isolate and image CTC in whole blood. By combining ferroferric oxide (FeO) nanoparticles modified with aptamer (Apt), poly[(9,9-dioctylfluorenyl-2,7-diyl)-alt-co-(1,4-benzo-{2,1',3}-thiadiazole)] (PFBT) and poly(styrene-co-maleic anhydride) (PSMA) formed in situ a large number of polymer dots (Pdots) on the surface of graphene oxide (GO), yielding a μm-sized 2D fluorescent probe Pdots@GO@FeO-Apt.

Flexible Double Bonds-Enhanced Photodynamic Therapy toward Antibacterial Resistance.

The relationship between the molecular structure and types of reactive oxygen species (ROS) generated from photosensitizers (PSs) must be understood for advancing photodynamic therapy (PDT). In this study, we synthesized , , and consisting of C-C, C═C, and C≡C bond linkers, respectively, between electron donor and acceptor moieties. Unlike and , containing a flexible C═C bond linker can absorb energy under white light irradiation, which enhances the swing of connected acceptor groups and leads to the formation of a noncoplanar structure.

Stereotactic infusion of rotenone into the SN induced a late-stage model of Parkinson's disease.

The establishment of an in vivo model for Parkinson's disease (PD) that accurately mimics late-stage clinical features is crucial for elucidating disease pathogenesis and developing novel therapeutic strategies. To establish this model, rotenone (ROT) was stereotactically infused into the substantia nigra (SN). Motor behavior in PD mice was assessed using the pole test and rotarod test.

Ternary Schottky-p-n heterojunction strategy for enhancing photothermal dry reforming of methane.

Breaking the trade-off between activity and stability in catalysts for dry reforming of methane has long remained a huge challenge. Here, we demonstrate a ternary Schottky-p-n (TSPN) heterojunction strategy based on Ni-NiO-SrNbO (NiO/SNO) for photothermal dry reforming of methane. This approach achieves a stable syngas production rate of 10.

The Burden of Nonmalignant Metabolic Dysfunction-Associated Steatotic Liver Disease in the Western Pacific Region: A Systematic Analysis From 1990 to 2021.

The burden of metabolic dysfunction-associated steatotic liver disease (MASLD) is increasing, yet there is little understanding in the Western Pacific Region (WPR). This study aims to report the latest MASLD burden in the WPR from 1990 to 2021. This study obtained nonmalignant and total MASLD incidence, prevalence, mortality, disability-adjusted life years (DALYs), years of life lost (YLLs), and years of healthy life lost due to disability (YLDs) from the Global Burden of Disease (GBD) database, and calculated age-standardized ratios (ASRs) for each indicator.

Synergistic bio-mechanical pretreatment and fed-batch semi-SSF strategy for enhanced cellulosic ethanol production from ramie straw.

The recalcitrant lignocellulosic matrix impedes biorefinery efficiency‌‌. This study developed a mechanical-biological pretreatment combined with fed-batch high-solids semi-simultaneous saccharification and fermentation (S-SSF) strategy for ethanol production from ramie straw. Results showed that dual fungal pretreatment with Pleurotus ostreatus (PO) and Ganoderma lucidum (GL) achieved selective lignin (59.

Biliary YB-1/GLI2 axis facilitates ductular reaction and promotes HSC activation via SPP1/integrin αvβ1 signaling during liver fibrogenesis.

Background And Aims: Emerging evidence suggests that ductular reactive cells (DRCs)-mediated ductular reaction (DR) accelerates the activation of HSCs and contributes to liver fibrogenesis. Previous studies implicated Y-box binding protein 1 (YB-1) in promoting DRC expansion. This study aims to investigate the mechanisms underlying YB-1-mediated DR and its role in HSC activation.

Epidemiology and prognosis of burn injuries in China: a meta-analysis.

Background: Burns are a significant cause of disability and death. Nonfatal burns have drastic impacts on survivors' lives. This study examines the epidemiological characteristics and prognostic factors of burn injuries of hospitalized patients in China.

[Clinical Characteristics and Prognostic Analysis of Peripheral T-Cell Lymphoma, Not Otherwise Specified].

Objective: To investigate the clinical characteristics and prognosis of peripheral T-cell lymphoma, not otherwise specified (PTCL-NOS).

Methods: Clinical data of 10 patients with PTCL-NOS in Gansu Provincial Hospital from May 2016 to June 2023 were collected. The treatment outcomes were evaluated, and the factors affecting prognosis were analyzed.

Targeting liposomes and an accelerated CRISPR system for selective imaging of miR-21 in cells.

This study demonstrates a liposome-targeted and accelerated CRISPR strategy for specific response of miR-21 within MCF-7 cells. Targeting was achieved with the help of modification of the MUC1 aptamer. MnO NS could be used as both a delivery vehicle and an accelerator for the CRISPR system, and finally to achieve spatially selective imaging of miR-21.

Serum syndecan-1 is related to severity and prognosis of COVID-19 patients: a perspective cohort study.

Introduction: Syndecan-1 is one of cell surface proteoglycans that mediates the connection between cytoskeleton and interstitial matrix. The previous studies have demonstrated that syndecan-1 exerts an essential role in several pulmonary diseases. However, there are few researches about the relation of serum syndecan-1 with corona virus disease 2019 (COVID-19).