Discovery of Zharp1-163 as a dual inhibitor of ferroptosis and necroptosis for the treatment of inflammatory disorders and kidney injury.

Dysregulation of cell death plays a critical role in the onset and progression of numerous human diseases. Distinct forms of regulated cell death, such as necroptosis and ferroptosis, have been implicated in the pathogenesis of various conditions, including neurodegenerative disorders and acute kidney injury. Strategies that concurrently target both necroptosis and ferroptosis present significant potential for improving therapeutic outcomes.

Comparative Analysis of Structural Characterisation and Gel Properties of Blended/Co-Precipitated Soy-Pea Dual-Protein.

This study proposed a pH-driven co-precipitation strategy to overcome the limitations of traditional physical blending in functional improvement of a dual-protein system. The results demonstrated that, in comparison with the soy-pea blended protein (SPBP), the soy-pea co-precipitated protein (SPCP) showed a decrease in α-helix and β-sheet content, accompanied by in an increase in random coil structure. SPCP exhibited decreased fluorescence intensity, smaller particle size (from 392.

Sensitive and Anti-Interference Al Sensing with a Luminescent Eu-MOF.

A luminescent lanthanide metal-organic framework, [(TD)Eu(HCO)(DMF)] (Eu-TD, TD/HBTDB = 4,4'-(benzo[][1,2,5] thiadiazole-4,7-diyl)dibenzoic acid), was synthesized via a solvothermal method by coordinating Eu ions with the strongly emissive ligand HBTDB. Single-crystal X-ray diffraction analysis reveals that the Eu-TD is in the hexagonal system with space group 622. Eu-TD demonstrated exceptional selectivity toward Al ions, exhibiting remarkable sensitivity with a low detection limit (limit of detection (LOD) = 0.

Brain functional network abnormalities in Parkinson's disease patients at different disease stages.

Background: Parkinson's disease (PD) is a neurodegenerative disorder with some progressive impairment and an unclear pathogenesis.

Purpose: This study aimed to use resting-state functional magnetic resonance imaging (rs-fMRI) and graph analysis approaches to compare changes in brain functional network topology in PD at different disease stages.

Materials And Methods: A total of 58 PD patients, comprising 29 early-stage PD (PD-E) and 29 middle-to-late stage PD (PD-M), and 29 age- and sex-matched healthy control (HC) participants, were recruited.

Immunotherapy and senolytics in head and neck squamous cell carcinoma: phase 2 trial results.

Recent advancements in cancer immunotherapy have improved patient outcomes, yet responses to immunotherapy remain moderate. Immunosenescence has been shown to contribute to the development and progression of various diseases; however, its specific role in solid tumors has not been fully delineated. Here we conducted a phase 2 clinical trial involving 51 patients with cancer undergoing neoadjuvant chemoimmunotherapy and applied single-cell RNA as well as TCR and BCR sequencing on tumor and blood samples to elucidate the immune cell perturbations.

B7-H3 and CSPG4-targeted CAR T cells as potent effectors in anaplastic thyroid cancer.

Background: Anaplastic thyroid cancer (ATC) is a rare and aggressive malignancy with poor survival and no available effective therapy. This unmet clinical need led us to investigate chimeric antigen receptor (CAR) T cells s as potential treatment option for this malignant disease. As target tumor antigens of our CAR T cell therapy, we selected the chondroitin sulfate proteoglycan 4 (CSPG4) and the B7-homolog 3 (B7-H3), as they are both highly and homogeneously expressed on different types of thyroid carcinoma cell lines and tissues, including ATC.

Research note: Phosphoproteomic analysis of macrophage immunity stimulated by egg white glycopeptide.

Egg white glycopeptides (EWG) are dietary peptides with immune regulatory activities; however, the underlying immune regulation mechanisms of EWG-mediated have not been fully elucidated. Phosphorylation represents a crucial protein modification in immune cell signaling. In this study, phosphoproteomic technology was employed to investigate alterations in macrophage phosphoprotein profiles in response to EWG stimulation (EWG group) using lipopolysaccharide stimulation as a positive control (LPS group), and bioinformatics approaches were used to analyze the associated signaling pathways.

Vitamin D deficiency in Hashimoto's thyroiditis: mechanisms, immune modulation, and therapeutic implications.

Background: To evaluate vitamin D's role in thyroid autoimmunity modulation, establish evidence-based supplementation protocols, and address surgical implications in Hashimoto's thyroiditis (HT) care. Vitamin D deficiency is prevalent in HT patients and correlates with accelerated autoimmune progression. This review synthesizes mechanistic insights and clinical implications of vitamin D repletion in HT management.

Soy protein isolate-acylated anthocyanins self-assembled nanoparticles enhanced bigels physicochemical properties: Effect of amphiphilic nanoparticles on the bigels system.

The amphiphilic nanoparticles prepared from soy protein isolate and acylated anthocyanins based on the Mannich reaction were innovatively incorporated into bigels. The bigels were prepared by beeswax oleogels and guar gum hydrogels, with systematic investigation of nanoparticles on the physical properties and stability. The results showed that the bigels belonged to hydrogels-in-organogels type, and the addition of amphiphilic nanoparticles acheived the dual effect of interfacial and morphological stability of the bigels.

Jianpi Qushi Heluo formula ameliorates podocytes injury related with ROS-mediated NLRP3 inflammasome activation in membranous nephropathy by promoting PINK1-dependent mitophagy.

Ethnopharmacological Relevance: Jianpi Qushi Heluo formula (JQHF) is an evidence-based herbal formula based on "Fangji Huangqi Decoction" in the classic of traditional Chinese medicine (TCM) synopsis of the Golden Chamber. Its effectiveness in reducing idiopathic membranous nephropathy (IMN) proteinuria, edema and other clinical symptoms and improving kidney injury has been confirmed by clinical trials and animal experiments. These results demonstrate the critical significance of reducing podocyte injury by regulating mitophagy through integrating ethnopharmacology.

Impact of iron ore mining activities on the rhizosphere fungal communities of , , and L.

Iron ore mining has damaged the natural environment and affected the ecological balance of the surrounding areas. The purpose of this study was to investigate the effects of iron ore mining on fungal communities in the rhizosphere soils of plantain, peach, and wheat. The results revealed that, due to iron ore mining, the pH value of the soil in the mining area generally exhibited weak acidity, and the Fe and TP contents increased significantly ( < 0.

Mature tertiary lymphoid structures linked to HPV status and anti-PD-1 based chemoimmunotherapy response in head and neck squamous cell carcinoma.

Mature tertiary lymphoid structures (TLSs) are immune aggregates associated with immune checkpoint blockade (ICB) responses in various cancers, yet their role in chemoimmunotherapy response in head and neck squamous cell carcinoma (HNSCC) remains unclear. By analyzing TCGA-HNSC transcriptomic data and pathology slides, we identified an immune subtype enriched in TLSs, predominantly in HPV-positive tumors, which correlated with favorable immunotherapy response. Single-cell and spatial transcriptomics further revealed distinct TLS compositions, with mature TLSs enriched in germinal center B cells, follicular helper T cells, and resident memory CD8 T cells, while immature TLSs contained FCRL4+ B cells and peripheral helper T cells.

Piperlongumine enhances the antitumor efficacy of PD-1 inhibitors by inducing immunogenic cell death in prostate cancer cells.

Background: In advanced prostate cancers, the immunologically "cold" tumor microenvironment inhibiting lymphocyte infiltration has been a significant barrier to the successful application of immune checkpoint inhibitor-based therapy. In other cancer types, immunogenic cell death inducers have been shown to restore sensitivity to checkpoint inhibitor-based therapy by promoting lymphocyte infiltration and activating naïve cytotoxic T lymphocytes through cross-presentation of neoantigens by dendritic cells. Here, we describe the ability of natural product and reactive oxygen species-inducer piperlongumine to induce immunogenic cell death in prostate cancer cells and synergize with PD-1 inhibitors in the treatment of mouse prostate cancer models.

Physicochemical properties, aroma compounds, non-volatile metabolites and their potential correlation in fermented Sichuan-style sausage.

To assess the modulation of on aroma compounds, non-volatile metabolites, and their potential correlation in Sichuan-style sausage, in this study, an isolated strain was used as starter cultures to prepare Sichuan-style sausages at different inoculum levels (10, 10, and 10 CFU/g). The results showed that fermented sausages exhibited reduced total plate counts and stronger antioxidant activity compared to control sausage. UHPLC-MS/MS metabolomics identified 1047 metabolites, of which 930 were characterized.

Overcoming resistance to immunotherapy by targeting CD38 in human tumor explants.

CD38, an ecto-enzyme involved in NAD catabolism, is highly expressed in exhausted CD8 T cells and has emerged as an attractive target to improve response to immune checkpoint blockade (ICB) by blunting T cell exhaustion. However, the precise role(s) and regulation of CD38 in exhausted T cells and the efficacy of CD38-directed therapeutic strategies in human cancer remain incompletely defined. Here, we show that CD38CD8 T cells are induced by chronic TCR activation and type I interferon stimulation and confirm their association with ICB resistance in human melanoma.

Rheological Characterization of Different Fat-Derived Products.

Background: Fat grafting has been widely used to correct soft-tissue volume loss and facial rejuvenation. Recent innovative advances have led to different types of fat-derived products. Although the composition of cells and extracellular matrix within different fat products has been reported, their physical and rheological properties are poorly defined.

Metabolomic Profiling and Antioxidant Capacity Changes in Longzi Black Barley During Germination.

Longzi black barley ( L. var. , LBB), a highland barley variant with superior nutritional properties, has gained increasing attention for its health-promoting benefits.

Overexpression of a (L.) Borkh WRKY Factor Gene Increased High Salinity Stress Tolerance in .

The WRKY transcription factor family is a significant family of plant transcription factors (TFs). Plant growth and development are often influenced by abiotic factors, such as salinity and low temperature. Numerous studies have demonstrated that WRKY TFs primarily influence plant responses to adversity.

High-resolution multiomics links nutrients and mixotrophy to toxicity in a harmful bloom of the haptophyte .

Harmful algal blooms (HABs) of the toxigenic haptophyte are known to cause fish mortalities and collateral ecosystem damage. The ichthyotoxic mechanisms are poorly understood but likely dependent on toxigenesis by polyketide synthases (PKSs). We hypothesize that induction of PKS activity facilitates mixotrophic behavior during nutrient-depleted bloom conditions.

Investigation of Meat Quality, Volatilome, and Fatty Acid Composition of Meat Parts from Liangshan Semi-Fine Wool Sheep.

Liangshan Semi-fine Wool Sheep (LSWS, ) are widely raised in Liangshan Yi Autonomous Prefecture, Sichuan, China. To provide a scientific basis for LSWS meat processing, our study investigated various parameters across six meat parts of LSWS including the neck, chuck roll, thin flank, outside flat, eye of round, and hind shank. Our findings revealed that thin flank displayed a higher pH compared to outside flat ( < 0.

A photosynthetic egg based oxygen-releasing platform to promote angiogenesis and tissue regeneration in diabetic wounds.

Diabetic wounds represent a longstanding global health challenge attributable to tissue hypoxia resulting from impaired microcirculation, which impedes crucial physiological processes essential for wound healing, such as cell proliferation and migration. Oxygen-releasing biomaterials present a novel avenue for tissue reoxygenation therapy, offering advantages over conventional hyperbaric oxygen therapy. Herein, we developed a microcosmic oxygen-releasing platform (MORP) named photosynthetic egg by utilizing egg white hydrogel with inherent bioactive factors for regenerative strength and electrostatic adsorbedbringing photosynthetic oxygen production.

Carbon components in organic amendments drive nitrogen metabolism in one-year-long anaerobic soil microcosms.

Introduction: Long-term studies on the dynamic changes in nitrogen metabolism and functional microbial communities under anaerobic conditions, particularly those driven by organic amendments, remain scarce.

Methods: We conducted a year-long anaerobic microcosm experiment using three organic amendments-aerobically fermented pig-manure digestate (ACM), compost (ACP) and straw powder (ACS)-alongside an inorganic fertilizer-only control (ACN).

Results: Temporal shifts revealed that organic amendments drove distinct nitrogen metabolism pathways.

Pilot-scale duckweed-CO wastewater bioremediation system: a synergistic approach for nutrient removal, carbon capture, and biomass production.

This study presents a pilot-scale duckweed-based wastewater treatment system enhanced with CO fertilization to improve nutrient removal, CO capture, and biomass productivity. Three strains of Landoltia punctata were cultivated continuously for five months. In spring, CO supplementation significantly increased the biomass yield and starch and protein productivity (by more than 12.

Turing Instability and Hopf Bifurcation in 2-D Coupled Cellular Neural Networks.

The dynamics of 2-D two-grid coupled cellular neural networks (CNNs) are considered. Assuming the boundary conditions of zero-flux type, the linearized model is analyzed by using the decoupling method, which is described as matrix operations, such as the Kronecker product and Kronecker sum. Then, the local stability of the zero equilibrium related to system parameters is studied.

Incidence and Risk Factors of Post-Lumbar Puncture Headache in Patients With Cognitive Impairment.

Background: Cognitive impairment is prevalent among the elderly population. Cerebrospinal fluid (CSF) biomarker tests are employed to facilitate timely and differential diagnosis of cognitive dysfunction.

Objective: To investigate incidence and risk factors of post-lumbar puncture headache (PLPH) in Chinese geriatric patients with cognitive impairment.