Impact of iron ore mining activities on the rhizosphere fungal communities of , , and L.

Iron ore mining has damaged the natural environment and affected the ecological balance of the surrounding areas. The purpose of this study was to investigate the effects of iron ore mining on fungal communities in the rhizosphere soils of plantain, peach, and wheat. The results revealed that, due to iron ore mining, the pH value of the soil in the mining area generally exhibited weak acidity, and the Fe and TP contents increased significantly ( < 0.

Explore the changes of metabolites in feces and serum of acute pancreatitis patients with different etiologies by LC-MS based metabolomics strategy.

Background: Acute pancreatitis (AP) is a common abdominal inflammatory disease, which is mainly caused by gallstones, hyperlipidemia, and so on. Previous studies have explored the changes of serum metabolites in patients with AP. However, whether different etiologies have distinct impacts on the fecal and serum metabolites of the AP patients is still in suspense.

A novel Bacillus velezensis strain with the ability to simultaneously biodegrade polystyrene microplastics and fungicide carbendazim.

In this study, a novel bacterial strain, M1, capable of degrading both polystyrene (PS) microplastics and carbendazim, was isolated from soil heavily contaminated with microplastics and fungicide carbendazim, and was identified and determined to be Bacillus velezensis. During the 60-day cultivation period, degradation experiments with single pollutants demonstrated that, at a concentration of 1000 mg/L, the strain achieved mass reduction efficiency of 10.8 ± 0.

Study on the degradation efficiency and mechanism of polystyrene microplastics by five kinds of edible fungi.

The harm caused by microplastics to the environment and human body has become a hot issue, and screening for microorganisms that potentially degrade microplastics is urgently needed. This study is the first to use Auricularia auricula, Pleurotus ostreatus, Ganoderma lucidum, Pleurotus pulmonarius and Pleurotus cornucopiae to degrade PS-MPs. After 50 d of degradation, the average degradation rate was approximately 7.

Assembly Mechanisms and Functional Adaptations of Soil Fungal Communities of Different Plant Rhizospheres in Ilmenite Mining Area.

This study investigated the mechanisms of structural assembly and functional adaptations of fungal communities in the rhizosphere soils of seven different plants grown in the ilmenite zone. We analyzed changes in the rhizosphere soil fungal communities using ITS sequencing. The results revealed that different plants affected the properties of the rhizosphere soil.

Three local plants adapt to ecological restoration of abandoned lead-zinc mines through assembly of rhizosphere bacterial communities.

Introduction: The plant restoration and ecological restoration of lead-zinc mines are very important.

Methods: In this study, we used three local plants to carry out ecological restoration of abandoned lead-zinc mining areas and detected the adaptive mechanisms of soil bacterial diversity and function during the ecological restoration of lead-zinc mines through 16S rRNA sequencing.

Results: The results revealed that lead-zinc mining significantly reduced the soil bacterial diversity, including the Shannon, Simpson, and observed species indices, whereas the planting of the three ecological restoration plants restored the soil microbial diversity to a certain extent, leading to increases in the Shannon index and Observed species indices.

Sources of Lipopeptides and Their Applications in Food and Human Health: A Review.

Lipopeptides (LPs) are widely sourced surface-active natural products with a wide range of functions and low toxicity, high potency, and good biodegradability. In this paper, we summarize, for the first time, the plant, animal, microbial, and synthetic sources of LPs. We also introduce the applications of LPs in food and human health, including (1) LPs can inhibit the growth of food microorganisms during production and preservation.

Profile of intestinal fungal microbiota in acute pancreatitis patients and healthy individuals.

Objective: The gut is involved in the development of acute pancreatitis (AP). Increased focus is being given to the role of gut microbiota in the pathogenesis of AP. Nevertheless, there is currently no available evidence regarding the composition of fungal microorganisms in the intestines of patients with AP.

[Characteristics of VOCs Emissions from Pesticide Use Sources Based on Pesticide Dosage Forms Analysis： A Case Study of Beijing].

To analyze the emission characteristics of VOCs from pesticide use sources in Beijing, the distribution of commonly used pesticides and dosage forms in Beijing was obtained through on-site research, and the VOC content of pesticides in different dosage forms was examined using laboratory testing methods. The emission factors of pesticide VOCs for localized dosage forms in Beijing were established, an inventory of pesticide use source VOCs was compiled, and the spatial and temporal distribution characteristics of pesticide use source VOCs were analyzed. The results indicated that ① Pesticide dosage forms were the main factors affecting the emission of VOCs from pesticides, and when accounting for VOC emissions from pesticide sources, it is necessary to know the types of pesticides and active ingredients in the target area and obtain information on pesticide dosage forms simultaneously.

Suppression of Wnt/β-catenin Signaling in PDAC via METTL16-mediated N6-methyladenosine Modification of DVL2.

Pancreatic cancer is a formidable cause of cancer-related deaths worldwide and has witnessed a more than twofold increase in incidence over the last 25 years. The most frequently occurring form of pancreatic cancer is pancreatic ductal adenocarcinoma (PDAC), accounting for the majority of pancreatic cancer cases. N6-methyladenosine (m6A), the most abundant transcript modification, has been implicated in the pathogenesis of numerous human cancers, including pancreatic cancer.

RELA-induced MiR-21 Exerts Oncogenic Effects on PDAC via Targeting of ARHGAP24.

Inflammation is one of the inducing factors of pancreatic ductal adenocarcinoma (PDAC), and microRNAs have been confirmed to be involved in the occurrence and development of PDAC. However, whether RELA, an inflammatory regulator, is involved in the regulation of PDAC by miRNA remains to be further studied. In the present study miR-21 was characterized and its upstream regulatory mechanism was investigated, as well as its functional effects and target genes in pancreatic ductal adenocarcinoma (PDAC).

The Wet Suction Technique Enhances the Diagnostic Efficacy and Aspirate Quality of EUS-FNA for Solid Lesions: A Multicenter Retrospective Study in China.

Goals: To comprehensively compare the wet suction technique with the conventional dry suction technique for endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) in solid lesions.

Background: Optimal suction techniques for EUS-FNA remain uncertain when approaching solid lesions.

Study: We performed a retrospective study of EUS-FNA at 3 medical centers in China.

Value of Crystal Vue technique in detecting the placenta accreta spectrum located in c-section scar area.

Aims: Excessive placental invasion is a life-threatening obstetric disease. Determining the extent of placental villi invasion prenatally is crucial for formulating a surgical plan for pregnant women. The objective of this study was to explore the diagnostic accuracy of the Crystal Vue technique combined with two-dimensional (2D) ultrasound in detecting the degree of placenta accreta spectrum (PAS) located in the C-section scar area.

Comparative assessment of the biocompatibility and degradation behavior of Zn-3Cu and JDBM alloys used for biliary surgery.

This study was designed to investigate the biocompatibility and the degradation behavior of a Zn-3Cu alloy, a Zn-3Cu coating alloy, a Mg-Nd-Zn-Zr (denoted as JDBM) alloy and a JDBM coating alloy to choose the optimal alloy for common bile duct (CBD) surgery. In the in vitro degradation experiments, we observed the surface morphology of the samples and determined the elements of the corrosion products. In the in vitro cytotoxicity experiments, the cell morphology and cytotoxicity were observed and tested.

Combined Real-Time Three-Dimensional Hysterosalpingo-Contrast Sonography with B Mode Hysterosalpingo-Contrast Sonography in the Evaluation of Fallopian Tube Patency in Patients Undergoing Infertility Investigations.

Objective: This prospective study aimed to investigate the use of real-time three-dimensional hysterosalpingo-contrast sonography (4D-HyCoSy), using contrast agent SonoVue, with B mode hysterosalpingo-contrast sonography (B mode-HyCoSy), to evaluate tubal patency and the wall of the Fallopian tubes in infertility patients.

Method: In total, we recruited 739 women with fertility requirements from the First Affiliated Hospital of Shantou Medical College between January 2017 and July 2018. All cases received 4D-HyCoSy using contrast agent SonoVue, immediately followed by the B mode-HyCoSy.

Applying WCACG modified process is beneficial on reduced door-to-balloon time of acute STEMI patients.

Background: Various systems have employed with the objective to reduce the time from emergency medical services contact to balloon inflammation for ST-elevation myocardial infraction (STEMI) patients. The WCACG message system was used to an alternative communication platform to improve confirmation of the diagnosis and movement to treatment, resulted in shorten the door-to-balloon (D-to-B) time for STEMI patients.

Methods: We collected 366 STEMI patients admitted at the Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Department of Cardiology, during the period from June 2013 to October 2015.

miR-21 promotes EGF-induced pancreatic cancer cell proliferation by targeting Spry2.

Pancreatic ductal adenocarcinoma (PDAC) is a highly malignant cancer that lacks effective targets for therapy. Alteration of epidermal growth factor (EGF) expression has been recognized as an essential molecular event in pancreatic carcinogenesis. Accumulating studies have demonstrated that miRNAs play critical roles in EGF signaling regulation, tumor initiation, cell proliferation and apoptosis.

The efficacy of temporary placement of nasobiliary drainage following endoscopic metal stenting to prevent post-ERCP cholangitis in patients with cholangiocarcinoma.

Background/aims: Although endoscopic metal biliary endoprosthesis (EMBE) is widely accepted as the most suitable drainage method for patients with unresectable malignant obstruction, uncontrolled post-procedural cholangitis is still a problem. We aimed to validate a new treatment modality to prevent post-ERCP cholangitis in patients with unresectable cholangiocarcinoma.

Patients And Methods: A total of 378 patients who were diagnosed with unresectable malignant biliary obstruction and underwent EMBE or temporary endoscopic nasobiliary drainage (ENBD) following EMBE placement, from January 2010 to July 2016, were enrolled in this retrospective study.

Dilation catheter-guided mini-forceps biopsy improves the diagnostic accuracy of malignant biliary strictures.

Background: Tissue sampling for biliary stricture is important for differential diagnosis and further treatment. The aim of this study was to assess a novel dilation catheter-guided mini-forceps biopsy (DCMB) method in the diagnosis of malignant biliary strictures.

Methods: 42 patients with malignant biliary stricture who underwent both brush cytology and DCMB during endoscopic retrograde cholangiopancreatography between October 2014 and November 2015 were retrospectively included.

Endoscopic ultrasonography-guided drainage combined with trans-duodenoscope cyclic irrigation technique for walled-off pancreatic necrosis.

Background: Endoscopic ultrasonography-guided drainage has been established as a good treatment modality in the management of walled-off pancreatic necrosis, but the unmanageable infection of postoperation is still a thorny problem due to the poor drainage ability for solid necrotic debris only through transmural stent and nasocystic catheter.

Aims: Introduce a novel therapeutic method, namely endoscopic ultrasonography-guided drainage combined with cyclic irrigation technique in managing patients with walled-off pancreatic necrosis.

Methods: 18 patients with severe acute pancreatitis complicated with walled-off pancreatic necrosis received treatment with endoscopic ultrasonography-guided drainage combined with cyclic irrigation were involved in this retrospective study.

Molecular cloning and functional characterization of a mouse gene upregulated by lipopolysaccharide treatment reveals alternative splicing.

Treatment of mouse cells with lipopolysaccharide (LPS) potently initiates an inflammatory response, but the underlying mechanisms are unclear. We therefore sought to characterize cDNA sequences of a new mouse LPS-responsive gene, and to evaluate the effects of MLrg. Full-length cDNAs were obtained from LPS-treated NIH3T3 cells.

Cloning and biological comparison of Restin, novel member of Mage superfamily.

In the present study, a new member of melanoma associated antigens (Mage), named Restin (219 amino acids), was identified from HL-60 cell induced by all-trans-retinoic acid (ATRA) by PCR-based subtractive hybridization. Bioinformatics analysis found this novel gene shares high homolog with Necdin (a neuronal growth suppressor, 49%). Both of them are basic proteins.

[Soluble expression, purification and bioactivity of recombinant human Era protein].

Aim: To prepare a soluble human Era(hEra) protein and to measure its bioactivity.

Methods: Human era cDNA gene from pUC19 plasmid was subcloned into the expression plasmid pMAL-p2x. pMAL-hEra was transducted to E.