Insight into the structural deterioration of biosynthesized holoferritin upon thermal treatment.

Holoferritin is considered a promising iron supplement, yet its preparation is challenging due to low extraction efficiencies from natural sources and the potential for structural damage during in vitro mineralization. This study reported the in vivo biosynthesis of a highly stable holoferritin (bs-holoFt) in Escherichia coli a high iron-loading capacity (1213 Fe atoms/protein) and systematically characterized the impact of heat treatments (70-100 °C) on the protein's multi-level structure and dual functions. Results showed a clear, temperature-dependent degradation pathway, initiated by the loss of α-helical content (decreased from 77.

Research on the construction method and characterization of neutralizing mouse-canine chimeric antibody against canine distemper virus.

Canine distemper (CD) is an acute infectious disease that poses significant health risks to canines. Neutralizing monoclonal antibody (mAb) therapy has demonstrated substantial efficacy in prevent CDV infection. However, immune rejection reactions prevent the use of mouse-derived mAbs in the prophylactic protection of CD.

Ultrabroadband Photosensitivity and Frequency-Mixing in Anisotropic Weyl Semimetal NbNiTe.

Broadband anisotropic photodetectors show great promise for polarization-sensitive imaging and multispectral optoelectronic systems yet face critical challenges in material anisotropy modulation and broadband sensitivity. Weyl semimetals exhibit giant optical anisotropy and tunable heterojunction band alignment, enabling high-performance anisotropic photodetection. Herein, ultrabroadband PDs based on the NbNiTe (niobium nickel telluride), enabled by antenna integration and heterostructure engineering, achieve high sensitivity from visible to Terahertz (THz).

Cisplatin-induced WWP1-eccDNA expression contributes to ovarian cancer resistance.

Background: Multidrug resistance remains a major obstacle in the treatment of ovarian cancer (OC) patients. Recent research has underscored the critical role of extrachromosomal circular DNA (eccDNA) in tumor initiation and progression. However, there is limited comprehensive understanding of the role eccDNA plays in tumor resistance.

Characterisation of tonoplast intrinsic proteins in birch: BpTIP1;3 confers drought tolerance by regulating the scavenging of reactive oxygen species.

Aquaporins (AQPs) are essential membrane channel proteins in plants, with tonoplast intrinsic proteins (TIPs) representing a subfamily of AQPs that play a crucial role in responses to abiotic stress. Here, we identified 10 BpTIP genes in birch (Betula platyphylla), distributed across seven chromosomes, all of which contain conserved major intrinsic protein domains. Polyethylene glycol-induced drought simulation induced the expression of all BpTIP genes except BpTIP5; 1.

Canopy gaps disturbance enhances ecosystem multifunctionality by increasing species and phylogenetic diversity in Picea schrenkiana forests.

Canopy gaps, as a common type of natural disturbance in forest ecosystems, drive biodiversity changes via niche differentiation, thereby effecting ecosystem multifunctionality (EMF). However, which dimension of biodiversity (e.g.

Exploring optimal serum selenium range for heart failure prevention: A U-shaped association and mediation by hepatic steatosis in US adults.

ObjectiveThe relationship between serum selenium levels and heart failure risk remains unclear. This study aimed to investigate the potential nonlinear association between serum selenium level and heart failure risk and explore whether hepatic steatosis and dyslipidemia mediate this relationship.MethodsData from 6969 adults in the National Health and Nutrition Examination Survey 2017-2020 cohort were analyzed.

Acid sphingomyelinase is a gatekeeper of placental labyrinthine architecture and function.

Sphingolipids are a class of bioactive signaling lipids that regulate an array of fundamental cellular processes, including cell survival, proliferation, and differentiation. Deficiency of acid sphingomyelinase-an enzyme of the sphingolipid metabolic pathway- has been previously implicated in human placental pathologies. We demonstrate that acid sphingomyelinase (Smpd1) is required for normal placental development in mouse, and its deficiency results in an intrauterine growth restriction phenotype.

How electronic health literacy influences physical activity behaviour among university students: A moderated mediation model.

Purpose: This study examines how eHealth literacy influences exercise behaviour among university students through a moderated mediation model. Specifically, peer relationships are positioned as mediators, while sleep quality moderates the effect of eHealth literacy on exercise behaviour among university students. The study explores the intricate interactions involved and elucidates how eHealth literacy affects exercise behaviour through multiple dimensions.

Mucosal Flap Reconstruction Enhances Survival in Salvage Endoscopic Nasopharyngectomy for Recurrent Nasopharyngeal Carcinoma: A Propensity-Matched Cohort Study.

Mucosal flap reconstruction can improve the long-term survival rate of patients with recurrent nasopharyngeal carcinoma. Mucosal flap reconstruction also increases the treatment-related risks of ear fullness and dysphagia. This matching study provides preliminary evidence for the use of mucosal flap reconstruction in salvage endoscopic nasopharyngectomy.

Targeting hematopoietic progenitor kinase 1 (HPK1) for tumor immunotherapy: advances in small molecule inhibitors.

Hematopoietic progenitor kinase 1 (HPK1), also known as MAP4K1, is a hematopoietic-specific serine/threonine kinase and a member of the MAP4K family of mammalian Ste20-associated protein kinases, which share a highly similar protein structure, and play important roles in the regulation of cell survival, cell migration, apoptosis and autophagy. HPK1 is a negative regulator of T-cell, B-cell and dendritic cell-mediated immune responses, and HPK1 kinase deficiency increases cytokine secretion and enhances T-cell signaling, viral clearance and tumor growth inhibition. Thus, HPK1 may be implicated in the development and progression of human malignant tumors and is a potent target for anti-tumor immunotherapy.

A water-energy-food-land nexus framework for multi-objective optimization and risk assessment integrating deep reinforcement learning and Copula-based modeling.

Climate change has intensified the interdependence within the water-energy-food-land nexus (WEFLN), and the resulting risk mechanism presents significant challenges to traditional resource management approaches. Current research faces challenges in quantifying the dependence structure of multi-resource shortage risks and lacks the capacity to perform synergistic optimization in high-dimensional decision spaces. This study proposes a Trinity framework for the WEFLN nexus, integrating R-vine Copula, Copula-based chance-constrained fuzzy multi-objective programming (CCFMOP), deep reinforcement learning, multi-objective evolutionary algorithm, and a coupling coordinated-gravity model (CCGM) to achieve a systematic process of risk identification-optimization decision-coordinated evaluation.

Menstrual blood-derived endometrial stem cells ameliorate neuroinflammation and apoptosis through JAK2/STAT3 signaling pathway in NPC1 mutant cell and mice.

Background: Niemann-Pick disease type C1 (NPC1) is caused by NPC1 gene mutations, resulting in Purkinje cell degeneration and death, glial cell activation, and progressive neurodegeneration. Menstrual blood-derived endometrial stem cells (MenSCs) have been explored as a promising tool for treating neurodegenerative diseases due to their wide range of sources, non-invasive nature, and regular collection methods.

Objectives: This study aims to investigate whether MenSCs can improve neuroinflammation and apoptosis in NPC1 mutant cell (Npc1 BV2 cell line) and mice (Npc1 mice), and explore their underlying mechanisms.

Apelin-13-Mediated Upregulation of METTL3 Ameliorates Alzheimer's Disease via Inhibiting Neuroinflammation Through m6A-Dependent Regulation of lncRNA BDNF-AS.

Apelin-13, a neuropeptide, has been recognized for its neuroprotective properties. Our previous study found apelin-13 improves cognitive function in Alzheimer's disease (AD) rats by inhibiting neuroinflammation through upregulation of BDNF/TrkB signaling pathway. However, the precise mechanism by which apelin-13 modulates BDNF remains unclear.

Parental Challenges in Raising Preschoolers With Attention-Deficit/Hyperactivity Disorder in Mainland China's First-Tier Cities: Qualitative Study Using Framework Analysis.

Background: First-tier cities in mainland China present unique challenges for raising children with attention-deficit/hyperactivity disorder (ADHD) due to rapid urbanization, intense academic pressure, and distinct sociocultural dynamics. While existing research has documented ADHD parenting challenges across various contexts, limited attention has been paid to the preschool period in Chinese metropolitan settings, where early intervention is crucial yet complicated by traditional values and evolving health care systems.

Objective: This study aimed to explore the comprehensive challenges faced by parents raising preschoolers with ADHD in these metropolitan contexts.

Diagnostic value of MR black-blood thrombus imaging, contrast-enhanced MRI, and noncontrast-enhanced MR venography in cerebral vein thrombosis: a systematic review and meta-analysis.

Objectives: The diagnosis of cerebral venous thrombosis (CVT) remains challenging due to limited consensus on the optimal imaging modality. MR black-blood thrombus imaging (MRBTI), contrast-enhanced MR imaging (CE-MRI), and noncontrast-enhanced MR venography (NCE-MRV) are promising modalities. This study aims to evaluate the performance of these modalities in diagnosing CVT.

Dietary protein sources in concentrate supplementation influence growth performance by manipulating gut microbiota and serum metabolites in suckling Donkey foals.

Background: Protein is a primary nutrient in concentrate supplementation for donkey foals, and the source of this protein significantly influences their growth and development. Milk-derived protein sources, such as milk powder, casein, and whey protein, are widely used in milk replacers for donkey foals due to their balanced nutritional profiles, high digestibility, and high bioavailability. However, the increasing costs of milk powder and whey protein have prompted researchers to explore alternative protein sources, with soy protein being a particularly promising option.

Modulating G6PD/PGD to overcome FSP1/DHODH-mediated ferroptosis defence: A novel oridonin derivative suppresses liver cancer.

Background: Hepatocellular carcinoma (HCC), a globally prevalent malignancy with high mortality rates, presents an unmet need for innovative effective therapies.

Purpose: This study aimed to explore the antitumour potential of compound XD, a novel oridonin derivative, on HCC and its underlying mechanism.

Experimental Approach: The antitumour effects of compound XD were investigated in several HCC cells lines and mice models.

Synergistic Enhancement of Anti-aging Effects on Human Umbilical Vein Endothelial Cells Treated With the Combination of Ferulic Acid and Rapamycin.

Introduction: Aging is a complex process involving cellular, genetic, metabolic, and mitochondrial changes. While significant progress has been made in understanding aging mechanisms and developing anti-aging drugs, single-drug treatments have limitations. This paper aims to investigate the synergistic effects of Ferulic acid (FA) and Rapamycin (Rapa) on anti-aging and to elucidate their underlying mechanisms, providing novel strategies for future anti-aging therapies.

Enhanced bi-branch deep learning network for in vivo hyperspectral imaging recognition of organs and tissues.

Hyperspectral imaging, as an emerging medical imaging technology, offers significant potential in biomedical research due to its ability in capturing rich spectral information. An enhanced bi-branch network integrating graph convolutional network (GCN) and transformer for hyperspectral image recognition of multiple organs and tissues was developed. GCN is able to extract regional information of same category adjacent to the target pixel, while transformer utilizes long-range dependencies to capture the boundary information.

FBXO42 promotes hepatocellular carcinoma progression via mediating p57Kip2 ubiquitination and degradation.

Background: Hepatocellular carcinoma (HCC), one of the most common malignancies, accounts for about 80% of all primary liver cancers. FBXO42 belongs to the F-box protein family and functions as a key component of the SCF (Skp1-Cullin-F-box) ubiquitin ligase complex, which is involved in the process of protein ubiquitination. The studies on the role of FBXO42 in different diseases are inadequate, especially in HCC.

The influence of physical activity on emotional management ability in college students: a chain mediating role of psychological resilience and health literacy.

Objective: This study aimed to investigate the impact of physical activity (PA) on emotional management ability (EMA) in college students and to analyze the chain mediating roles of psychological resilience (PR) and health literacy (HL) in the relationship between PA and EMA.

Methods: Demographic information was collected using a stratified, cluster, and multi-stage sampling method. Data on PA, PR, HL, and EMA among college students were collected using an online questionnaire.

Integrative speckle-tracking echocardiography for the early detection and prediction of cancer therapy-related cardiac dysfunction.

Background: The cancer therapy–related cardiac dysfunction (CTRCD) induced by anthracycline could involve the whole heart. However, the overall changes of strain parameters, including the atrial and right heart strains, myocardial work, along with the established left ventricular global longitudinal strain (GLS), during cancer therapy, remain unclear.

Methods: In this prospective cohort study, 83 women undergoing anthracycline treatment for breast cancer, underwent serial echocardiographic examinations at baseline (pre-chemotherapy, T0), after 1 to 4 cycles (T1-T4) and 3 months after treatment completion (T5).

A Pure Spin Current in Organic Materials with Ratchet Mechanism.

The magnetic ratchet experiment demonstrates that directional spin diffusion can occur even in the absence of a net driving field. Inspired by these experiments, we utilize the Marcus theory to simulate spin-dependent charge hopping within a magnetic ratchet potential. Our results show that directional spin diffusion occurs only when both spatial and temporal conditions are satisfied simultaneously.

Complement pathway: Risk molecular pattern of cardiovascular diseases in patients with diabetic nephropathy.

Background: Among individuals with diabetes mellitus, diabetic nephropathy (DN) is a common microvascular complication. As renal dysfunction progresses in DN patients, the risk of cardiovascular disease (CVD) significantly increases. The current effective treatment of DN and CVD demands identifying and managing their risk factors.