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To assess the modulation of on aroma compounds, non-volatile metabolites, and their potential correlation in Sichuan-style sausage, in this study, an isolated strain was used as starter cultures to prepare Sichuan-style sausages at different inoculum levels (10, 10, and 10 CFU/g). The results showed that fermented sausages exhibited reduced total plate counts and stronger antioxidant activity compared to control sausage. UHPLC-MS/MS metabolomics identified 1047 metabolites, of which 930 were characterized. Five metabolic pathways were significantly regulated by . GC-IMS identified 157 aroma compounds, with significant increases of all aromas except acids in -fermented sausages. Strong correlations were observed among physicochemical properties, common differential metabolites and differential aroma compounds (|r| > 0.8, < 0.05). Twenty-two common differential metabolites were screened likely contributing to aroma formation. This study provides theoretical support for application of in improving the quality of Sichuan-style sausage.
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http://dx.doi.org/10.1016/j.fochx.2025.102642 | DOI Listing |
Int Microbiol
September 2025
School of Basic Sciences, Technology and Engineering, National Open and Distance University, Pasto, Nariño, Colombia.
This study investigates the impact of a defined starter culture on the fermentation of cocoa beans and its influence on the production of volatile and non-volatile compounds related to sensory quality. A microbial consortium comprising Saccharomyces cerevisiae, Pichia kudriavzevii, Levilactobacillus brevis, and Acetobacter okinawensis was selected based on their enzymatic activity and acid regulation properties. Fermentation trials showed that the starter culture enhanced the synthesis of key volatile compounds, particularly esters and higher alcohols, such as 2-phenylethanol and 2-phenylethyl acetate, which contribute floral and fruity aromas.
View Article and Find Full Text PDFJ Agric Food Chem
September 2025
PhyMedExp - Inserm U1046 - CNRS UMR 9214, CHU Arnaud de Villeneuve Bâtiment Crastes de Paulet, 371 avenue du Doyen Gaston Giraud, Montpellier Cedex 05 34295, France.
Different precursors of volatile sulfur compounds (VSCs) are present in fermented beverages, such as wine and beer. Carbon-sulfur (CS) lyases are enzymes that play a crucial role in releasing aromas from these varietal thiol precursors. These enzymes are expressed by various organisms, including yeasts and bacteria, involved in fermentation processes during brewing and winemaking.
View Article and Find Full Text PDFFront Plant Sci
August 2025
College of Agriculture, South China Agricultural University, Guangzhou, China.
Tobacco ( L.) is well-known as an economic crop whose quality is evaluated according to its aroma quality. Researchers have found that selenium application can increase the aroma quality of tobacco, but until now, its mechanism is still unclear.
View Article and Find Full Text PDFFood Sci Biotechnol
October 2025
Engineering Research Center of Storage and Processing of Xinjiang Characteristic Fruits and Vegetables, Ministry of Education, School of Food Science, Shihezi University, Shihezi, 832000 Xinjiang China.
The current work aimed to investigate the effects of fermentation of , , and on the physicochemical, electronic sensory evaluation, and flavour characteristics of heat-sterilized tomato juice (HTJ). The results indicated that LAB fermentation significantly decreased the pH, sucrose, and glucose, and lactic acid was increased. E-nose and tongue analyses revealed that the response to organic sulfides, terpenoids, and sourness increased after LAB fermentation HS-SPME-GC-MS and OAV revealed that heat-sterilization resulted a significant loss of aroma compounds (38.
View Article and Find Full Text PDF3 Biotech
October 2025
Key Laboratory of Green Manufacturing and Biosynthesis of Food Bioactive Substances, China General Chamber of Commerce, School of Food and Health, Beijing Technology and Business University, Beijing, 100048 China.
Unlabelled: Soy whey, a tofu production byproduct rich in nutrients, is typically discarded. This study investigated five natural lactic acid bacteria (LAB) strains isolated from spontaneously fermented soy whey (FSW) for their fermentation performance in soy whey medium. The growth characteristics, total phenols and flavonoids compounds, antioxidant activities, and aroma profiles were evaluated.
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