ZnCe-LDO Nanozyme-Based Multifunctional Hydrogel Promotes Bone Regeneration by Inflammatory Macrophage Reprogramming and Piezo1 Activation.

Prolonged or excessive inflammation may lead to impaired vascularization and bone regeneration, hindering the normal repair process of bone tissue. Although the regulation of inflammation is crucial for promoting a conducive microenvironment for bone regeneration, individual anti-inflammatory interventions frequently are inadequate in facilitating effective bone repair. Here, a multifunctional hydrogel (GelMA-ZC-Yoda1) with multifaceted therapeutic strategy was designed by integrating Zinc/Cerium-layered double oxide nanozyme (ZnCe-LDO, with catalase-like activity) and Yoda1 (an activator of the mechanically sensitive Piezo1 ion channel) into photocurable GelMA hydrogel.

Chelerythrine Inhibits TGF-β-Induced Epithelial-Mesenchymal Transition in A549 Cells via RRM2.

The mechanisms underlying the metastasis of non-small-cell lung cancer (NSCLC) have long been a focal point of medical research. The anti-tumor effects of chelerythrine (CHE) have been confirmed; however, its ability to inhibit tumor metastasis and the underlying mechanisms remain unknown. The aim of this study was to investigate the inhibitory effects and molecular mechanisms of CHE on transforming growth factor-beta (TGF-β)-induced epithelial-mesenchymal transition (EMT).

Developing a golgi-targeted fluorescent probe for in situ visualization of the dynamic changes of HS in cellular stress.

The Golgi apparatus, as a crucial component of the endomembrane system in eukaryotic cells, serves as a central hub for intracellular macromolecular transport, playing a key role in receiving, modifying, and transporting proteins and lipids. Its dysfunction is closely associated with the pathogenesis of various diseases. This study focuses on the pivotal role of the Golgi apparatus in cellular stress responses and its regulatory mechanisms, particularly the protective function of the gas signaling molecule hydrogen sulfide (HS) during Golgi stress.

A Robust and Orthogonal Far-Red Light Sensor for Gene Expression Control in .

Optogenetics has emerged as a powerful tool for regulating cellular processes due to its noninvasive nature and precise spatiotemporal control. Far-red light (FRL) has increasingly been used in the optogenetic control of mammalian cells due to its low toxicity and high tissue penetration. However, robust and orthogonal FRL sensors are lacking in bacteria.

De-starched Panax ginseng polysaccharide: Preparation, in vitro digestion, fermentation properties and the activating effect of the resultant products on RAW264.7 cells.

Panax ginseng C. A. Meyer (ginseng) neutral polysaccharides have been proven to be an immune enhancer, but their digestion and fermentation characteristics are unclear.

Structural characterization and immunostimulant activities of polysaccharides fractionated by gradient ethanol precipitation method from C. A. Meyer.

C. A. Meyer is a dual-purpose plant for medicine and food, its polysaccharide is considered as an immune enhancer.

Rapid and facile detection of largemouth bass ranavirus with CRISPR/Cas13a.

Largemouth bass ranavirus (LMBV) is an epidemic disease that seriously jeopardizes the culture of largemouth bass（Micropterus salmoides）, and it has a very high incidence in largemouth bass. Once an outbreak occurs, it may directly lead to the failure of the culture, resulting in substantial economic losses, but there is no effective vaccine or special effective drug yet. Consequently, it is important to establish an accurate, sensitive, convenient and specific detection approach for preventing LMBV infection.

[Spatial and temporal variation in normalized difference vegetation index of vegetation in Liaoning Province from the perspective of ecogeographic zoning].

Normalized difference vegetation index (NDVI) is an important indicator reflecting vegetation cover and growth status. It is of significance for regional ecological conservation and natural resource management to investigate its spatial and temporal variation trends and response to ecological factors. We divided Liaoning Province into three ecological geographical regions, including northwest agro-pastoral zone, central agricultural zone, and eastern agroforestry zone.

Order Matters When Using Two-Sided Messages to Influence Morally Based Attitudes.

Contrary to common beliefs, sometimes downplaying or even undermining one's case can enhance impact, especially for people with strong attitudes. Across four studies ( = 1,548), we demonstrate that the placement of the undermining information within a two-sided message matters. By manipulating message order within a two-sided message, Study 1 showed that the relative effectiveness of two- over one-sided messages for people with a moral attitude primarily occurred when the two-sided message acknowledged the recipient's side at the end rather than at the beginning of the message.

Rapid detection of grass carp reovirus type 1 using RPA-based test strips combined with CRISPR Cas13a system.

Introduction: Due to the existence of grass carp reovirus (GCRV), grass carp hemorrhagic disease occurs frequently, and its high pathogenicity and infectivity are great challenges to the aquaculture industry. As a highly pathogenic pathogen, the outbreak of hemorrhagic disease often causes tremendous economic losses. Therefore, it is important to rapidly and accurately detect GCRV on site to control timely.

A neutral heteropolysaccharide from Halenia elliptica D. Don: Extraction, structural characterization, antioxidant and antiaging activities.

Halenia elliptica D. Don (H. elliptica), which is also known as "heijicao" and "luanehuamao" in China, is recognised as a valuable Tibetan medicinal plant with polysaccharides as the main active ingredient.

Transdermal delivery of brucine-encapsulated liposomes significantly enhances anti-tumor outcomes in treating triple-negative breast cancer.

Triple-negative breast cancer (TNBC) is always the most challenging breast cancer subtype. Herein, brucine, encapsulated in peptide-modified liposomes, was proposed for treating TNBC by transdermal delivery. For the TD peptide-modified brucine-loaded liposome (Bru-TD-Lip) we developed, it presents high encapsulation efficiency of brucine and stability.

Structural characteristics of mixed pectin from ginseng berry and its anti-obesity effects by regulating the intestinal flora.

Ginseng berry is the mature berry of ginseng and its polysaccharide has hypolipidaemic effect, but its mechanism remains unclear. A pectin (GBPA) with a molecular weight of 3.53 × 10 Da was isolated from ginseng berry, it was mainly composed of Rha (25.

Bacteria-based multiplex system eradicates recurrent infections with drug-resistant bacteria via photothermal killing and protective immunity elicitation.

Background: The high mortality associated with drug-resistant bacterial infections is an intractable clinical problem resulting from the low susceptibility of these bacteria to antibiotics and the high incidence of recurrent infections.

Methods: Herein, a photosynthetic bacteria-based multiplex system (Rp@Al) composed of natural Rhodopseudomonas palustris (Rp) and Food and Drug Administration-approved aluminum (Al) adjuvant, was developed to combat drug-resistant bacterial infections and prevent their recurrence. We examined its photothermal performance and in vitro and in vivo antibacterial ability; revealed its protective immunomodulatory effect; verified its preventative effect on recurrent infections; and demonstrated the system's safety.

Dual-Functional Nanoplatform Based on Bimetallic Metal-Organic Frameworks for Synergistic Starvation and Chemodynamic Therapy.

Tumor microenvironment (TME)-responsive chemodynamic therapy (CDT) mediated by nanozymes has been extensively studied in oral squamous cell carcinoma. However, the low catalytic efficiency due to insufficient HO in the TME is still a major challenge for its clinical translation. Herein, we present an antitumor nanoplatform based on a Mn-Co organometallic framework material (MnCoMOF), which shows peroxidase-like (POD-like) activity, loaded with glucose oxidase (GOx@MnCoMOF), demonstrating the ability of HO self-supply and HO conversion to toxic hydroxyl radicals.

Structure and potential anti-fatigue mechanism of polysaccharides from Bupleurum chinense DC.

Two polysaccharides, BCP-1 and BCP-2, were obtained from Bupleurum chinense DC. by water extraction and ultrafiltration. BCP-1 (1.

Two-Sided Messages Promote Openness for a Variety of Deeply Entrenched Attitudes.

Prior research showed that people holding attitudes on relatively moral topics became more open to two- rather than one-sided messages as the moral basis of their attitudes increased. Across three studies ( = 963), we extend this finding to relatively non-moral topics by demonstrating that two-sided messages can encourage people with strong attitudes indexed by various non-moral attitude strength measures to be more open to contrary positions. Study 1 demonstrated this for four indicators of attitude strength (e.

Synergistic Microbicidal Effect of AUR and PEITC Against Skin Infection.

Given the increasing prevalence of antibiotic resistance, there is an urgent need to repurpose approved drugs with known pharmacology and toxicology as an alternative therapeutic strategy. We have reported that the sustained monotherapy of auranofin (AUR) inevitably resulted in reduced susceptibility or even the emergence of resistance to AUR in However, whether drug combination could increase antibacterial activity while preventing AUR resistance is still unknown. Here, we focused on the important role of AUR combined with phenethyl isothiocyanate (PEITC) in skin infection and determined the synergistic antimicrobial effect on by using checkerboard assays and time-kill kinetics analysis.

A novel hydrophobically associating water-soluble polymer used as constant rheology agent for cement slurry.

During the process of well cementing in deep water, the cement slurry experiences a wide range of temperature variation from low temperature at seabed to high temperature in downhole. The elevated temperature affects the rheology of cement slurry. The change of rheology of cement slurry could influence the safety of cementing operation.

Doxofylline Protects Gram-Negative Pathogens against Antibiotic-Mediated Killing.

Given the growing rate of Gram-negative bacterial infections, antibiotics are now frequently prescribed for various respiratory diseases. Doxofylline is a newer generation xanthine with both bronchodilating and anti-inflammatory activities, but its influence on antibiotics remains poorly understood. Here, we first report the discovery of doxofylline-induced high minimum inhibitory concentrations of antibiotics.

Gut Microbiota Contributes to Host Defense Against -Induced Liver Abscess.

Purpose: -induced liver abscess (KLA) is a type of pyogenic liver abscess (PLA), which is a distinct invasive syndrome that has been increasingly reported worldwide over the past two decades. The intestinal microbiota is increasingly recognized as an important modulator that can promote and maintain host immune homeostasis. However, its precise role in liver abscess is unknown.

Individual differences in three aspects of evaluation: The motives to have, learn, and express attitudes.

We report seven studies that introduce and validate two unique aspects of evaluation that supplement the original Need to Evaluate (NE) scale (Jarvis & Petty, 1996). Whereas the original scale focused on the tendency to have attitudes, the two new scales focus on the tendencies to learn and express attitudes. Although the new scales are correlated with the original scale and each other, each new scale is shown to be related in a unique way to other relevant scales, and most importantly, to predict different outcomes.

An iron oxide nanoparticle-based transdermal nanoplatform for dual-modal imaging-guided chemo-photothermal therapy of superficial tumors.

Transdermal delivery is an attractive strategy for treating superficial tumors. However, the applications of existing transdermal systems have been limited by low transdermal efficiency and poor therapeutic outcomes. Here, we develop a transdermal nanoplatform (+)T-SiDs, based on superparamagnetic iron oxide core, surface-modified with cationic lipids, transdermal enhanced peptide TD, and 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR), and loaded with doxorubicin.

Two-Sided Messages Promote Openness for Morally Based Attitudes.

This research demonstrates that two- versus one-sided counterattitudinal messages can encourage people with a strong moral basis for their attitudes to be more open to contrary positions. Studies 1A/B demonstrated that the interaction between moral basis and message sidedness was present not just for a controversial issue with balanced views in society but also for a topic with a majority opinion. In Study 2, the relative effectiveness of two- over one-sided messages for people with a moral attitude basis was shown to occur only when the two-sided message respectfully acknowledged the recipient's side.

Polysaccharide Improves LPS-Induced Senescence of RAW264.7 Cells by Regulating the NF-B Signaling Pathway.

Macrophages are important inflammatory cells that play a vital role in inflamm-aging. polysaccharide (BCP), an effective component of the herb, exerts multiple beneficial pharmacological effects, such as improving immunity and antioxidant activity. However, the effects of BCP on macrophage-aging and inflamm-aging are yet to be established.